Amino acid dipepetide frequency for Pseudomonas phage Ps56

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.556AlaAla: 17.556 ± 2.778
1.469AlaCys: 1.469 ± 0.398
6.651AlaAsp: 6.651 ± 0.537
8.507AlaGlu: 8.507 ± 0.832
3.944AlaPhe: 3.944 ± 0.647
10.131AlaGly: 10.131 ± 1.106
1.16AlaHis: 1.16 ± 0.307
4.872AlaIle: 4.872 ± 0.487
5.104AlaLys: 5.104 ± 0.712
13.457AlaLeu: 13.457 ± 1.117
3.635AlaMet: 3.635 ± 0.561
3.016AlaAsn: 3.016 ± 0.54
5.491AlaPro: 5.491 ± 0.708
6.265AlaGln: 6.265 ± 0.794
7.425AlaArg: 7.425 ± 0.826
6.806AlaSer: 6.806 ± 0.685
5.878AlaThr: 5.878 ± 0.718
6.651AlaVal: 6.651 ± 0.776
2.707AlaTrp: 2.707 ± 0.4
2.398AlaTyr: 2.398 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.851CysAla: 0.851 ± 0.3
0.309CysCys: 0.309 ± 0.151
0.773CysAsp: 0.773 ± 0.237
0.619CysGlu: 0.619 ± 0.198
0.387CysPhe: 0.387 ± 0.203
0.773CysGly: 0.773 ± 0.262
0.387CysHis: 0.387 ± 0.214
0.155CysIle: 0.155 ± 0.111
0.387CysLys: 0.387 ± 0.188
0.464CysLeu: 0.464 ± 0.201
0.077CysMet: 0.077 ± 0.08
0.387CysAsn: 0.387 ± 0.171
0.619CysPro: 0.619 ± 0.207
0.387CysGln: 0.387 ± 0.181
0.773CysArg: 0.773 ± 0.301
0.851CysSer: 0.851 ± 0.271
0.541CysThr: 0.541 ± 0.218
0.619CysVal: 0.619 ± 0.244
0.077CysTrp: 0.077 ± 0.076
0.232CysTyr: 0.232 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
6.883AspAla: 6.883 ± 0.805
0.232AspCys: 0.232 ± 0.135
3.171AspAsp: 3.171 ± 0.473
4.563AspGlu: 4.563 ± 0.546
2.398AspPhe: 2.398 ± 0.396
6.187AspGly: 6.187 ± 0.74
1.083AspHis: 1.083 ± 0.357
2.475AspIle: 2.475 ± 0.437
1.392AspLys: 1.392 ± 0.303
5.182AspLeu: 5.182 ± 0.543
1.237AspMet: 1.237 ± 0.294
1.624AspAsn: 1.624 ± 0.306
3.016AspPro: 3.016 ± 0.518
3.79AspGln: 3.79 ± 0.548
3.635AspArg: 3.635 ± 0.63
2.398AspSer: 2.398 ± 0.342
3.248AspThr: 3.248 ± 0.394
4.563AspVal: 4.563 ± 0.468
0.619AspTrp: 0.619 ± 0.17
1.392AspTyr: 1.392 ± 0.319
0.0AspXaa: 0.0 ± 0.0
Glu
7.425GluAla: 7.425 ± 1.107
0.696GluCys: 0.696 ± 0.248
3.79GluAsp: 3.79 ± 0.496
4.331GluGlu: 4.331 ± 0.58
1.701GluPhe: 1.701 ± 0.328
2.32GluGly: 2.32 ± 0.349
1.624GluHis: 1.624 ± 0.417
3.016GluIle: 3.016 ± 0.451
3.558GluLys: 3.558 ± 0.61
7.038GluLeu: 7.038 ± 0.733
1.547GluMet: 1.547 ± 0.363
1.933GluAsn: 1.933 ± 0.415
1.779GluPro: 1.779 ± 0.416
4.95GluGln: 4.95 ± 0.694
6.342GluArg: 6.342 ± 0.884
3.403GluSer: 3.403 ± 0.638
1.856GluThr: 1.856 ± 0.375
3.48GluVal: 3.48 ± 0.584
1.315GluTrp: 1.315 ± 0.307
1.701GluTyr: 1.701 ± 0.289
0.0GluXaa: 0.0 ± 0.0
Phe
2.707PheAla: 2.707 ± 0.423
0.232PheCys: 0.232 ± 0.134
2.784PheAsp: 2.784 ± 0.552
1.701PheGlu: 1.701 ± 0.348
0.773PhePhe: 0.773 ± 0.246
3.171PheGly: 3.171 ± 0.409
0.464PheHis: 0.464 ± 0.146
1.237PheIle: 1.237 ± 0.295
0.773PheLys: 0.773 ± 0.241
2.63PheLeu: 2.63 ± 0.373
0.696PheMet: 0.696 ± 0.21
1.469PheAsn: 1.469 ± 0.344
0.696PhePro: 0.696 ± 0.186
1.315PheGln: 1.315 ± 0.355
2.398PheArg: 2.398 ± 0.48
1.779PheSer: 1.779 ± 0.348
1.701PheThr: 1.701 ± 0.37
1.392PheVal: 1.392 ± 0.303
0.464PheTrp: 0.464 ± 0.175
0.464PheTyr: 0.464 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
8.121GlyAla: 8.121 ± 1.279
1.005GlyCys: 1.005 ± 0.279
4.408GlyAsp: 4.408 ± 0.659
4.254GlyGlu: 4.254 ± 0.473
2.243GlyPhe: 2.243 ± 0.295
5.027GlyGly: 5.027 ± 0.615
1.547GlyHis: 1.547 ± 0.334
3.248GlyIle: 3.248 ± 0.409
4.563GlyLys: 4.563 ± 0.642
7.657GlyLeu: 7.657 ± 0.747
2.32GlyMet: 2.32 ± 0.395
3.094GlyAsn: 3.094 ± 0.491
2.475GlyPro: 2.475 ± 0.471
4.563GlyGln: 4.563 ± 0.629
4.563GlyArg: 4.563 ± 0.658
3.403GlySer: 3.403 ± 0.528
3.635GlyThr: 3.635 ± 0.535
5.336GlyVal: 5.336 ± 0.573
2.011GlyTrp: 2.011 ± 0.449
2.552GlyTyr: 2.552 ± 0.448
0.0GlyXaa: 0.0 ± 0.0
His
2.088HisAla: 2.088 ± 0.401
0.155HisCys: 0.155 ± 0.102
0.696HisAsp: 0.696 ± 0.201
1.083HisGlu: 1.083 ± 0.273
0.387HisPhe: 0.387 ± 0.166
1.701HisGly: 1.701 ± 0.405
0.309HisHis: 0.309 ± 0.141
0.928HisIle: 0.928 ± 0.226
0.696HisLys: 0.696 ± 0.205
2.088HisLeu: 2.088 ± 0.507
0.155HisMet: 0.155 ± 0.11
0.619HisAsn: 0.619 ± 0.222
1.083HisPro: 1.083 ± 0.332
1.547HisGln: 1.547 ± 0.516
1.315HisArg: 1.315 ± 0.326
1.392HisSer: 1.392 ± 0.376
0.541HisThr: 0.541 ± 0.2
1.237HisVal: 1.237 ± 0.297
0.155HisTrp: 0.155 ± 0.089
0.155HisTyr: 0.155 ± 0.115
0.0HisXaa: 0.0 ± 0.0
Ile
5.491IleAla: 5.491 ± 0.722
0.464IleCys: 0.464 ± 0.181
3.403IleAsp: 3.403 ± 0.454
4.176IleGlu: 4.176 ± 0.547
0.696IlePhe: 0.696 ± 0.24
2.784IleGly: 2.784 ± 0.503
1.005IleHis: 1.005 ± 0.254
1.083IleIle: 1.083 ± 0.355
1.315IleLys: 1.315 ± 0.257
3.558IleLeu: 3.558 ± 0.469
0.387IleMet: 0.387 ± 0.187
1.547IleAsn: 1.547 ± 0.42
2.088IlePro: 2.088 ± 0.399
1.547IleGln: 1.547 ± 0.314
2.862IleArg: 2.862 ± 0.411
2.011IleSer: 2.011 ± 0.578
3.403IleThr: 3.403 ± 0.625
1.856IleVal: 1.856 ± 0.398
0.541IleTrp: 0.541 ± 0.202
1.005IleTyr: 1.005 ± 0.323
0.0IleXaa: 0.0 ± 0.0
Lys
5.491LysAla: 5.491 ± 0.835
0.309LysCys: 0.309 ± 0.185
2.088LysAsp: 2.088 ± 0.424
2.32LysGlu: 2.32 ± 0.482
0.851LysPhe: 0.851 ± 0.265
2.862LysGly: 2.862 ± 0.531
0.619LysHis: 0.619 ± 0.161
1.392LysIle: 1.392 ± 0.348
1.701LysLys: 1.701 ± 0.335
3.944LysLeu: 3.944 ± 0.592
0.387LysMet: 0.387 ± 0.172
1.083LysAsn: 1.083 ± 0.275
2.63LysPro: 2.63 ± 0.576
1.547LysGln: 1.547 ± 0.397
4.408LysArg: 4.408 ± 0.634
2.475LysSer: 2.475 ± 0.449
2.475LysThr: 2.475 ± 0.456
2.32LysVal: 2.32 ± 0.393
0.387LysTrp: 0.387 ± 0.163
0.696LysTyr: 0.696 ± 0.201
0.0LysXaa: 0.0 ± 0.0
Leu
12.761LeuAla: 12.761 ± 0.997
0.928LeuCys: 0.928 ± 0.273
7.347LeuAsp: 7.347 ± 0.733
5.491LeuGlu: 5.491 ± 0.622
2.398LeuPhe: 2.398 ± 0.435
7.27LeuGly: 7.27 ± 0.755
2.011LeuHis: 2.011 ± 0.341
3.48LeuIle: 3.48 ± 0.58
3.712LeuLys: 3.712 ± 0.622
8.507LeuLeu: 8.507 ± 0.803
2.088LeuMet: 2.088 ± 0.379
3.016LeuAsn: 3.016 ± 0.488
5.104LeuPro: 5.104 ± 0.53
5.414LeuGln: 5.414 ± 0.765
8.043LeuArg: 8.043 ± 0.955
5.8LeuSer: 5.8 ± 0.722
6.419LeuThr: 6.419 ± 0.754
7.657LeuVal: 7.657 ± 0.737
0.773LeuTrp: 0.773 ± 0.226
2.398LeuTyr: 2.398 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
3.326MetAla: 3.326 ± 0.458
0.0MetCys: 0.0 ± 0.0
1.237MetAsp: 1.237 ± 0.325
1.701MetGlu: 1.701 ± 0.364
0.387MetPhe: 0.387 ± 0.153
2.011MetGly: 2.011 ± 0.372
0.464MetHis: 0.464 ± 0.231
1.392MetIle: 1.392 ± 0.365
1.083MetLys: 1.083 ± 0.324
1.624MetLeu: 1.624 ± 0.343
0.155MetMet: 0.155 ± 0.095
0.696MetAsn: 0.696 ± 0.211
0.928MetPro: 0.928 ± 0.271
1.083MetGln: 1.083 ± 0.273
1.392MetArg: 1.392 ± 0.295
1.779MetSer: 1.779 ± 0.342
1.083MetThr: 1.083 ± 0.273
1.005MetVal: 1.005 ± 0.26
0.232MetTrp: 0.232 ± 0.126
0.077MetTyr: 0.077 ± 0.064
0.0MetXaa: 0.0 ± 0.0
Asn
3.558AsnAla: 3.558 ± 0.476
0.077AsnCys: 0.077 ± 0.08
1.083AsnAsp: 1.083 ± 0.271
1.701AsnGlu: 1.701 ± 0.386
0.696AsnPhe: 0.696 ± 0.224
3.326AsnGly: 3.326 ± 0.733
0.696AsnHis: 0.696 ± 0.224
0.928AsnIle: 0.928 ± 0.308
0.928AsnLys: 0.928 ± 0.282
3.79AsnLeu: 3.79 ± 0.565
0.387AsnMet: 0.387 ± 0.137
0.696AsnAsn: 0.696 ± 0.228
1.701AsnPro: 1.701 ± 0.362
1.779AsnGln: 1.779 ± 0.403
2.166AsnArg: 2.166 ± 0.382
1.624AsnSer: 1.624 ± 0.376
1.469AsnThr: 1.469 ± 0.405
1.315AsnVal: 1.315 ± 0.25
0.541AsnTrp: 0.541 ± 0.21
1.237AsnTyr: 1.237 ± 0.306
0.0AsnXaa: 0.0 ± 0.0
Pro
7.27ProAla: 7.27 ± 0.748
0.309ProCys: 0.309 ± 0.155
3.48ProAsp: 3.48 ± 0.598
2.707ProGlu: 2.707 ± 0.383
1.469ProPhe: 1.469 ± 0.319
3.944ProGly: 3.944 ± 0.837
0.387ProHis: 0.387 ± 0.196
1.624ProIle: 1.624 ± 0.326
1.547ProLys: 1.547 ± 0.341
4.022ProLeu: 4.022 ± 0.608
1.315ProMet: 1.315 ± 0.309
1.237ProAsn: 1.237 ± 0.254
2.32ProPro: 2.32 ± 0.521
1.624ProGln: 1.624 ± 0.358
3.867ProArg: 3.867 ± 0.521
2.784ProSer: 2.784 ± 0.416
2.707ProThr: 2.707 ± 0.419
2.939ProVal: 2.939 ± 0.591
0.851ProTrp: 0.851 ± 0.251
2.011ProTyr: 2.011 ± 0.403
0.0ProXaa: 0.0 ± 0.0
Gln
7.038GlnAla: 7.038 ± 0.99
0.464GlnCys: 0.464 ± 0.2
2.32GlnAsp: 2.32 ± 0.457
3.326GlnGlu: 3.326 ± 0.615
2.166GlnPhe: 2.166 ± 0.434
2.707GlnGly: 2.707 ± 0.475
0.773GlnHis: 0.773 ± 0.25
1.933GlnIle: 1.933 ± 0.266
1.779GlnLys: 1.779 ± 0.525
6.961GlnLeu: 6.961 ± 0.799
0.928GlnMet: 0.928 ± 0.303
0.928GlnAsn: 0.928 ± 0.302
3.326GlnPro: 3.326 ± 0.496
3.403GlnGln: 3.403 ± 0.618
3.48GlnArg: 3.48 ± 0.48
2.552GlnSer: 2.552 ± 0.372
2.166GlnThr: 2.166 ± 0.376
3.79GlnVal: 3.79 ± 0.468
0.696GlnTrp: 0.696 ± 0.192
1.16GlnTyr: 1.16 ± 0.367
0.0GlnXaa: 0.0 ± 0.0
Arg
6.961ArgAla: 6.961 ± 0.803
0.696ArgCys: 0.696 ± 0.24
4.718ArgAsp: 4.718 ± 0.483
4.486ArgGlu: 4.486 ± 0.636
1.856ArgPhe: 1.856 ± 0.354
4.872ArgGly: 4.872 ± 0.707
2.243ArgHis: 2.243 ± 0.454
3.944ArgIle: 3.944 ± 0.489
3.248ArgLys: 3.248 ± 0.702
8.817ArgLeu: 8.817 ± 0.877
1.315ArgMet: 1.315 ± 0.326
1.701ArgAsn: 1.701 ± 0.439
2.475ArgPro: 2.475 ± 0.485
3.867ArgGln: 3.867 ± 0.68
6.419ArgArg: 6.419 ± 0.794
4.872ArgSer: 4.872 ± 0.584
2.243ArgThr: 2.243 ± 0.388
4.486ArgVal: 4.486 ± 0.605
1.315ArgTrp: 1.315 ± 0.334
2.398ArgTyr: 2.398 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
7.966SerAla: 7.966 ± 0.881
0.387SerCys: 0.387 ± 0.175
3.016SerAsp: 3.016 ± 0.464
3.403SerGlu: 3.403 ± 0.446
2.088SerPhe: 2.088 ± 0.351
4.64SerGly: 4.64 ± 0.557
0.619SerHis: 0.619 ± 0.187
2.398SerIle: 2.398 ± 0.377
2.32SerLys: 2.32 ± 0.545
5.336SerLeu: 5.336 ± 0.545
1.392SerMet: 1.392 ± 0.331
1.779SerAsn: 1.779 ± 0.302
2.939SerPro: 2.939 ± 0.565
2.011SerGln: 2.011 ± 0.48
3.403SerArg: 3.403 ± 0.584
3.171SerSer: 3.171 ± 0.523
3.635SerThr: 3.635 ± 0.441
3.171SerVal: 3.171 ± 0.502
1.237SerTrp: 1.237 ± 0.386
1.547SerTyr: 1.547 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
6.806ThrAla: 6.806 ± 0.823
0.696ThrCys: 0.696 ± 0.3
2.475ThrAsp: 2.475 ± 0.395
2.63ThrGlu: 2.63 ± 0.467
1.547ThrPhe: 1.547 ± 0.345
5.646ThrGly: 5.646 ± 0.914
1.005ThrHis: 1.005 ± 0.277
1.469ThrIle: 1.469 ± 0.341
1.701ThrLys: 1.701 ± 0.397
4.95ThrLeu: 4.95 ± 0.767
0.928ThrMet: 0.928 ± 0.241
1.856ThrAsn: 1.856 ± 0.338
3.635ThrPro: 3.635 ± 0.604
1.779ThrGln: 1.779 ± 0.405
2.939ThrArg: 2.939 ± 0.432
3.326ThrSer: 3.326 ± 0.449
3.016ThrThr: 3.016 ± 0.637
4.022ThrVal: 4.022 ± 0.719
0.928ThrTrp: 0.928 ± 0.3
1.469ThrTyr: 1.469 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
6.729ValAla: 6.729 ± 0.755
0.619ValCys: 0.619 ± 0.212
4.099ValAsp: 4.099 ± 0.5
4.64ValGlu: 4.64 ± 0.708
1.469ValPhe: 1.469 ± 0.299
3.79ValGly: 3.79 ± 0.573
1.16ValHis: 1.16 ± 0.298
3.558ValIle: 3.558 ± 0.693
2.552ValLys: 2.552 ± 0.391
6.11ValLeu: 6.11 ± 0.694
1.16ValMet: 1.16 ± 0.265
1.392ValAsn: 1.392 ± 0.293
3.79ValPro: 3.79 ± 0.488
2.784ValGln: 2.784 ± 0.466
4.022ValArg: 4.022 ± 0.575
3.558ValSer: 3.558 ± 0.512
4.331ValThr: 4.331 ± 0.575
4.872ValVal: 4.872 ± 0.777
1.237ValTrp: 1.237 ± 0.294
1.624ValTyr: 1.624 ± 0.537
0.0ValXaa: 0.0 ± 0.0
Trp
1.469TrpAla: 1.469 ± 0.329
0.309TrpCys: 0.309 ± 0.144
0.464TrpAsp: 0.464 ± 0.176
0.309TrpGlu: 0.309 ± 0.16
0.773TrpPhe: 0.773 ± 0.196
0.928TrpGly: 0.928 ± 0.295
0.232TrpHis: 0.232 ± 0.133
1.005TrpIle: 1.005 ± 0.285
0.464TrpLys: 0.464 ± 0.155
2.166TrpLeu: 2.166 ± 0.329
0.696TrpMet: 0.696 ± 0.236
0.464TrpAsn: 0.464 ± 0.177
0.541TrpPro: 0.541 ± 0.23
1.16TrpGln: 1.16 ± 0.314
1.392TrpArg: 1.392 ± 0.257
1.005TrpSer: 1.005 ± 0.276
1.315TrpThr: 1.315 ± 0.357
1.469TrpVal: 1.469 ± 0.301
0.464TrpTrp: 0.464 ± 0.259
0.232TrpTyr: 0.232 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.707TyrAla: 2.707 ± 0.477
0.309TyrCys: 0.309 ± 0.153
1.083TyrAsp: 1.083 ± 0.286
1.856TyrGlu: 1.856 ± 0.362
0.619TyrPhe: 0.619 ± 0.209
1.933TyrGly: 1.933 ± 0.395
0.541TyrHis: 0.541 ± 0.28
1.083TyrIle: 1.083 ± 0.314
1.16TyrLys: 1.16 ± 0.281
2.32TyrLeu: 2.32 ± 0.365
0.773TyrMet: 0.773 ± 0.249
1.16TyrAsn: 1.16 ± 0.278
1.779TyrPro: 1.779 ± 0.464
1.083TyrGln: 1.083 ± 0.278
2.088TyrArg: 2.088 ± 0.366
1.469TyrSer: 1.469 ± 0.408
1.16TyrThr: 1.16 ± 0.288
1.315TyrVal: 1.315 ± 0.351
0.309TyrTrp: 0.309 ± 0.137
0.309TyrTyr: 0.309 ± 0.136
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12931 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski