Amino acid dipepetide frequency for Mycobacterium phage KayaCho

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.976AlaAla: 16.976 ± 1.599
0.844AlaCys: 0.844 ± 0.184
8.266AlaAsp: 8.266 ± 0.614
10.621AlaGlu: 10.621 ± 0.77
2.666AlaPhe: 2.666 ± 0.309
10.044AlaGly: 10.044 ± 1.237
1.955AlaHis: 1.955 ± 0.385
4.755AlaIle: 4.755 ± 0.501
3.377AlaLys: 3.377 ± 0.391
11.288AlaLeu: 11.288 ± 1.069
3.155AlaMet: 3.155 ± 0.359
3.111AlaAsn: 3.111 ± 0.448
8.133AlaPro: 8.133 ± 0.693
5.333AlaGln: 5.333 ± 0.494
8.755AlaArg: 8.755 ± 0.735
6.355AlaSer: 6.355 ± 0.715
7.733AlaThr: 7.733 ± 0.722
8.799AlaVal: 8.799 ± 0.654
2.222AlaTrp: 2.222 ± 0.359
2.444AlaTyr: 2.444 ± 0.344
0.0AlaXaa: 0.0 ± 0.0
Cys
1.289CysAla: 1.289 ± 0.313
0.178CysCys: 0.178 ± 0.115
0.889CysAsp: 0.889 ± 0.285
0.267CysGlu: 0.267 ± 0.1
0.4CysPhe: 0.4 ± 0.152
1.511CysGly: 1.511 ± 0.333
0.222CysHis: 0.222 ± 0.105
0.222CysIle: 0.222 ± 0.113
0.0CysLys: 0.0 ± 0.0
0.267CysLeu: 0.267 ± 0.147
0.222CysMet: 0.222 ± 0.099
0.178CysAsn: 0.178 ± 0.096
1.2CysPro: 1.2 ± 0.413
0.444CysGln: 0.444 ± 0.18
1.067CysArg: 1.067 ± 0.315
0.489CysSer: 0.489 ± 0.166
0.4CysThr: 0.4 ± 0.159
0.489CysVal: 0.489 ± 0.169
0.222CysTrp: 0.222 ± 0.116
0.089CysTyr: 0.089 ± 0.056
0.0CysXaa: 0.0 ± 0.0
Asp
7.822AspAla: 7.822 ± 0.559
0.933AspCys: 0.933 ± 0.313
4.266AspAsp: 4.266 ± 0.459
4.044AspGlu: 4.044 ± 0.646
1.289AspPhe: 1.289 ± 0.243
6.044AspGly: 6.044 ± 0.61
1.555AspHis: 1.555 ± 0.325
2.533AspIle: 2.533 ± 0.324
1.467AspLys: 1.467 ± 0.258
4.711AspLeu: 4.711 ± 0.45
1.378AspMet: 1.378 ± 0.294
1.244AspAsn: 1.244 ± 0.239
5.155AspPro: 5.155 ± 0.501
3.066AspGln: 3.066 ± 0.551
4.4AspArg: 4.4 ± 0.531
2.444AspSer: 2.444 ± 0.298
3.822AspThr: 3.822 ± 0.482
4.444AspVal: 4.444 ± 0.412
1.644AspTrp: 1.644 ± 0.315
1.378AspTyr: 1.378 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
8.088GluAla: 8.088 ± 0.688
0.933GluCys: 0.933 ± 0.261
2.844GluAsp: 2.844 ± 0.415
2.978GluGlu: 2.978 ± 0.372
1.778GluPhe: 1.778 ± 0.242
5.111GluGly: 5.111 ± 0.558
0.889GluHis: 0.889 ± 0.252
2.933GluIle: 2.933 ± 0.423
1.155GluLys: 1.155 ± 0.244
5.999GluLeu: 5.999 ± 0.56
1.333GluMet: 1.333 ± 0.233
1.289GluAsn: 1.289 ± 0.295
4.311GluPro: 4.311 ± 0.568
2.533GluGln: 2.533 ± 0.39
3.955GluArg: 3.955 ± 0.497
2.444GluSer: 2.444 ± 0.272
4.4GluThr: 4.4 ± 0.414
5.066GluVal: 5.066 ± 0.53
1.378GluTrp: 1.378 ± 0.247
1.778GluTyr: 1.778 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.666PheAla: 2.666 ± 0.388
0.222PheCys: 0.222 ± 0.095
2.666PheAsp: 2.666 ± 0.38
1.244PheGlu: 1.244 ± 0.261
0.444PhePhe: 0.444 ± 0.15
2.889PheGly: 2.889 ± 0.45
0.622PheHis: 0.622 ± 0.187
1.067PheIle: 1.067 ± 0.274
0.8PheLys: 0.8 ± 0.182
1.911PheLeu: 1.911 ± 0.341
0.267PheMet: 0.267 ± 0.138
0.711PheAsn: 0.711 ± 0.18
1.022PhePro: 1.022 ± 0.272
0.933PheGln: 0.933 ± 0.225
1.733PheArg: 1.733 ± 0.329
1.067PheSer: 1.067 ± 0.241
1.644PheThr: 1.644 ± 0.276
2.266PheVal: 2.266 ± 0.348
0.356PheTrp: 0.356 ± 0.117
0.267PheTyr: 0.267 ± 0.123
0.0PheXaa: 0.0 ± 0.0
Gly
10.666GlyAla: 10.666 ± 1.337
0.844GlyCys: 0.844 ± 0.259
5.911GlyAsp: 5.911 ± 0.476
5.022GlyGlu: 5.022 ± 0.457
2.355GlyPhe: 2.355 ± 0.468
10.977GlyGly: 10.977 ± 2.042
1.644GlyHis: 1.644 ± 0.344
3.511GlyIle: 3.511 ± 0.37
2.622GlyLys: 2.622 ± 0.27
8.888GlyLeu: 8.888 ± 1.15
2.0GlyMet: 2.0 ± 0.298
3.289GlyAsn: 3.289 ± 0.497
3.511GlyPro: 3.511 ± 0.386
4.311GlyGln: 4.311 ± 0.475
6.488GlyArg: 6.488 ± 0.6
4.933GlySer: 4.933 ± 0.513
5.733GlyThr: 5.733 ± 0.711
6.666GlyVal: 6.666 ± 0.638
2.178GlyTrp: 2.178 ± 0.361
2.666GlyTyr: 2.666 ± 0.381
0.0GlyXaa: 0.0 ± 0.0
His
1.867HisAla: 1.867 ± 0.281
0.222HisCys: 0.222 ± 0.127
0.978HisAsp: 0.978 ± 0.235
0.533HisGlu: 0.533 ± 0.176
0.489HisPhe: 0.489 ± 0.162
1.555HisGly: 1.555 ± 0.293
0.578HisHis: 0.578 ± 0.179
0.667HisIle: 0.667 ± 0.178
0.356HisLys: 0.356 ± 0.137
1.467HisLeu: 1.467 ± 0.261
0.533HisMet: 0.533 ± 0.145
0.4HisAsn: 0.4 ± 0.145
1.555HisPro: 1.555 ± 0.311
0.578HisGln: 0.578 ± 0.144
2.044HisArg: 2.044 ± 0.335
0.4HisSer: 0.4 ± 0.123
1.422HisThr: 1.422 ± 0.303
1.067HisVal: 1.067 ± 0.23
0.356HisTrp: 0.356 ± 0.143
0.444HisTyr: 0.444 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
5.022IleAla: 5.022 ± 0.428
0.178IleCys: 0.178 ± 0.092
3.377IleAsp: 3.377 ± 0.363
3.289IleGlu: 3.289 ± 0.419
0.755IlePhe: 0.755 ± 0.159
4.089IleGly: 4.089 ± 0.521
0.622IleHis: 0.622 ± 0.233
1.955IleIle: 1.955 ± 0.29
1.378IleLys: 1.378 ± 0.311
2.489IleLeu: 2.489 ± 0.314
0.444IleMet: 0.444 ± 0.135
1.511IleAsn: 1.511 ± 0.272
2.889IlePro: 2.889 ± 0.468
1.244IleGln: 1.244 ± 0.259
2.755IleArg: 2.755 ± 0.352
2.355IleSer: 2.355 ± 0.333
3.511IleThr: 3.511 ± 0.431
3.377IleVal: 3.377 ± 0.331
0.267IleTrp: 0.267 ± 0.121
0.844IleTyr: 0.844 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
3.644LysAla: 3.644 ± 0.467
0.222LysCys: 0.222 ± 0.107
1.555LysAsp: 1.555 ± 0.359
1.244LysGlu: 1.244 ± 0.291
0.578LysPhe: 0.578 ± 0.173
2.4LysGly: 2.4 ± 0.341
0.267LysHis: 0.267 ± 0.109
1.511LysIle: 1.511 ± 0.319
0.8LysLys: 0.8 ± 0.182
2.4LysLeu: 2.4 ± 0.378
0.4LysMet: 0.4 ± 0.122
0.889LysAsn: 0.889 ± 0.145
1.289LysPro: 1.289 ± 0.302
0.844LysGln: 0.844 ± 0.223
1.6LysArg: 1.6 ± 0.225
1.555LysSer: 1.555 ± 0.269
2.355LysThr: 2.355 ± 0.304
1.867LysVal: 1.867 ± 0.265
0.578LysTrp: 0.578 ± 0.171
0.444LysTyr: 0.444 ± 0.16
0.0LysXaa: 0.0 ± 0.0
Leu
11.821LeuAla: 11.821 ± 0.84
0.578LeuCys: 0.578 ± 0.212
4.666LeuAsp: 4.666 ± 0.423
4.222LeuGlu: 4.222 ± 0.399
1.689LeuPhe: 1.689 ± 0.297
8.266LeuGly: 8.266 ± 0.998
1.333LeuHis: 1.333 ± 0.211
4.177LeuIle: 4.177 ± 0.458
1.867LeuLys: 1.867 ± 0.295
5.999LeuLeu: 5.999 ± 0.564
2.0LeuMet: 2.0 ± 0.306
1.778LeuAsn: 1.778 ± 0.345
5.6LeuPro: 5.6 ± 0.707
2.444LeuGln: 2.444 ± 0.418
4.533LeuArg: 4.533 ± 0.547
4.222LeuSer: 4.222 ± 0.386
7.244LeuThr: 7.244 ± 0.597
5.111LeuVal: 5.111 ± 0.476
1.2LeuTrp: 1.2 ± 0.263
1.733LeuTyr: 1.733 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
3.022MetAla: 3.022 ± 0.386
0.356MetCys: 0.356 ± 0.135
1.467MetAsp: 1.467 ± 0.248
0.755MetGlu: 0.755 ± 0.168
0.667MetPhe: 0.667 ± 0.165
1.822MetGly: 1.822 ± 0.316
0.356MetHis: 0.356 ± 0.143
0.978MetIle: 0.978 ± 0.214
0.667MetLys: 0.667 ± 0.19
1.289MetLeu: 1.289 ± 0.233
0.4MetMet: 0.4 ± 0.142
0.444MetAsn: 0.444 ± 0.124
1.6MetPro: 1.6 ± 0.222
0.533MetGln: 0.533 ± 0.161
1.511MetArg: 1.511 ± 0.217
1.555MetSer: 1.555 ± 0.203
2.933MetThr: 2.933 ± 0.323
1.111MetVal: 1.111 ± 0.228
0.267MetTrp: 0.267 ± 0.115
0.489MetTyr: 0.489 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
3.511AsnAla: 3.511 ± 0.466
0.311AsnCys: 0.311 ± 0.111
2.0AsnAsp: 2.0 ± 0.28
1.333AsnGlu: 1.333 ± 0.336
0.622AsnPhe: 0.622 ± 0.139
3.733AsnGly: 3.733 ± 0.461
0.667AsnHis: 0.667 ± 0.189
0.978AsnIle: 0.978 ± 0.281
0.711AsnLys: 0.711 ± 0.152
1.911AsnLeu: 1.911 ± 0.308
0.711AsnMet: 0.711 ± 0.175
0.711AsnAsn: 0.711 ± 0.187
2.0AsnPro: 2.0 ± 0.268
0.578AsnGln: 0.578 ± 0.168
1.333AsnArg: 1.333 ± 0.311
1.067AsnSer: 1.067 ± 0.219
2.0AsnThr: 2.0 ± 0.262
1.689AsnVal: 1.689 ± 0.281
0.444AsnTrp: 0.444 ± 0.126
0.755AsnTyr: 0.755 ± 0.16
0.0AsnXaa: 0.0 ± 0.0
Pro
7.377ProAla: 7.377 ± 0.665
0.489ProCys: 0.489 ± 0.168
5.022ProAsp: 5.022 ± 0.526
5.688ProGlu: 5.688 ± 0.765
1.911ProPhe: 1.911 ± 0.302
6.711ProGly: 6.711 ± 0.616
0.844ProHis: 0.844 ± 0.212
2.711ProIle: 2.711 ± 0.312
1.778ProLys: 1.778 ± 0.254
3.822ProLeu: 3.822 ± 0.396
1.378ProMet: 1.378 ± 0.311
1.689ProAsn: 1.689 ± 0.277
5.022ProPro: 5.022 ± 0.596
1.378ProGln: 1.378 ± 0.252
3.689ProArg: 3.689 ± 0.451
3.289ProSer: 3.289 ± 0.489
4.222ProThr: 4.222 ± 0.399
5.822ProVal: 5.822 ± 0.479
1.244ProTrp: 1.244 ± 0.245
0.844ProTyr: 0.844 ± 0.195
0.0ProXaa: 0.0 ± 0.0
Gln
5.2GlnAla: 5.2 ± 0.531
0.444GlnCys: 0.444 ± 0.176
1.022GlnAsp: 1.022 ± 0.191
1.111GlnGlu: 1.111 ± 0.256
1.022GlnPhe: 1.022 ± 0.247
2.889GlnGly: 2.889 ± 0.385
0.489GlnHis: 0.489 ± 0.125
2.089GlnIle: 2.089 ± 0.331
0.578GlnLys: 0.578 ± 0.181
3.955GlnLeu: 3.955 ± 0.49
0.933GlnMet: 0.933 ± 0.198
1.022GlnAsn: 1.022 ± 0.268
2.311GlnPro: 2.311 ± 0.373
0.889GlnGln: 0.889 ± 0.227
2.8GlnArg: 2.8 ± 0.403
1.111GlnSer: 1.111 ± 0.24
2.311GlnThr: 2.311 ± 0.305
3.333GlnVal: 3.333 ± 0.392
0.489GlnTrp: 0.489 ± 0.188
0.8GlnTyr: 0.8 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
8.177ArgAla: 8.177 ± 0.725
0.933ArgCys: 0.933 ± 0.288
4.622ArgAsp: 4.622 ± 0.453
4.133ArgGlu: 4.133 ± 0.458
1.778ArgPhe: 1.778 ± 0.237
4.844ArgGly: 4.844 ± 0.501
1.422ArgHis: 1.422 ± 0.292
2.044ArgIle: 2.044 ± 0.32
2.089ArgLys: 2.089 ± 0.286
6.266ArgLeu: 6.266 ± 0.608
2.0ArgMet: 2.0 ± 0.326
1.555ArgAsn: 1.555 ± 0.224
4.311ArgPro: 4.311 ± 0.596
2.355ArgGln: 2.355 ± 0.322
5.955ArgArg: 5.955 ± 0.771
3.733ArgSer: 3.733 ± 0.371
3.377ArgThr: 3.377 ± 0.393
5.288ArgVal: 5.288 ± 0.746
1.689ArgTrp: 1.689 ± 0.331
1.644ArgTyr: 1.644 ± 0.323
0.0ArgXaa: 0.0 ± 0.0
Ser
6.755SerAla: 6.755 ± 0.453
0.356SerCys: 0.356 ± 0.133
2.266SerAsp: 2.266 ± 0.354
2.489SerGlu: 2.489 ± 0.462
0.889SerPhe: 0.889 ± 0.245
5.377SerGly: 5.377 ± 0.659
0.755SerHis: 0.755 ± 0.189
2.089SerIle: 2.089 ± 0.303
1.511SerLys: 1.511 ± 0.269
3.822SerLeu: 3.822 ± 0.474
0.889SerMet: 0.889 ± 0.188
1.289SerAsn: 1.289 ± 0.215
3.377SerPro: 3.377 ± 0.447
1.911SerGln: 1.911 ± 0.342
3.289SerArg: 3.289 ± 0.377
2.311SerSer: 2.311 ± 0.369
3.333SerThr: 3.333 ± 0.319
4.622SerVal: 4.622 ± 0.456
0.978SerTrp: 0.978 ± 0.222
1.022SerTyr: 1.022 ± 0.303
0.0SerXaa: 0.0 ± 0.0
Thr
9.732ThrAla: 9.732 ± 0.748
0.578ThrCys: 0.578 ± 0.172
4.8ThrAsp: 4.8 ± 0.587
5.155ThrGlu: 5.155 ± 0.576
2.089ThrPhe: 2.089 ± 0.332
6.977ThrGly: 6.977 ± 0.524
1.067ThrHis: 1.067 ± 0.202
2.444ThrIle: 2.444 ± 0.302
2.4ThrLys: 2.4 ± 0.309
5.288ThrLeu: 5.288 ± 0.505
1.422ThrMet: 1.422 ± 0.266
1.6ThrAsn: 1.6 ± 0.249
4.844ThrPro: 4.844 ± 0.723
1.822ThrGln: 1.822 ± 0.249
4.577ThrArg: 4.577 ± 0.456
3.733ThrSer: 3.733 ± 0.436
4.044ThrThr: 4.044 ± 0.508
4.844ThrVal: 4.844 ± 0.467
2.266ThrTrp: 2.266 ± 0.354
1.289ThrTyr: 1.289 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
8.133ValAla: 8.133 ± 0.757
0.8ValCys: 0.8 ± 0.241
4.577ValAsp: 4.577 ± 0.495
4.888ValGlu: 4.888 ± 0.37
1.867ValPhe: 1.867 ± 0.294
5.866ValGly: 5.866 ± 0.609
1.289ValHis: 1.289 ± 0.248
4.089ValIle: 4.089 ± 0.394
1.911ValLys: 1.911 ± 0.257
5.333ValLeu: 5.333 ± 0.479
1.733ValMet: 1.733 ± 0.277
3.022ValAsn: 3.022 ± 0.481
4.355ValPro: 4.355 ± 0.433
2.266ValGln: 2.266 ± 0.306
4.266ValArg: 4.266 ± 0.435
4.133ValSer: 4.133 ± 0.431
7.333ValThr: 7.333 ± 0.809
5.066ValVal: 5.066 ± 0.599
1.155ValTrp: 1.155 ± 0.241
1.467ValTyr: 1.467 ± 0.243
0.0ValXaa: 0.0 ± 0.0
Trp
2.133TrpAla: 2.133 ± 0.376
0.444TrpCys: 0.444 ± 0.155
1.111TrpAsp: 1.111 ± 0.24
0.889TrpGlu: 0.889 ± 0.21
0.978TrpPhe: 0.978 ± 0.297
0.889TrpGly: 0.889 ± 0.179
0.578TrpHis: 0.578 ± 0.187
0.933TrpIle: 0.933 ± 0.186
0.444TrpLys: 0.444 ± 0.119
1.689TrpLeu: 1.689 ± 0.278
0.444TrpMet: 0.444 ± 0.143
0.755TrpAsn: 0.755 ± 0.196
1.333TrpPro: 1.333 ± 0.263
0.533TrpGln: 0.533 ± 0.193
1.467TrpArg: 1.467 ± 0.304
1.2TrpSer: 1.2 ± 0.245
1.778TrpThr: 1.778 ± 0.297
0.889TrpVal: 0.889 ± 0.222
0.578TrpTrp: 0.578 ± 0.193
0.711TrpTyr: 0.711 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.933TyrAla: 2.933 ± 0.337
0.178TyrCys: 0.178 ± 0.093
1.333TyrAsp: 1.333 ± 0.22
1.289TyrGlu: 1.289 ± 0.231
0.533TyrPhe: 0.533 ± 0.167
2.222TyrGly: 2.222 ± 0.398
0.356TyrHis: 0.356 ± 0.141
0.667TyrIle: 0.667 ± 0.18
0.533TyrLys: 0.533 ± 0.143
1.689TyrLeu: 1.689 ± 0.258
0.533TyrMet: 0.533 ± 0.125
0.578TyrAsn: 0.578 ± 0.204
1.155TyrPro: 1.155 ± 0.27
0.8TyrGln: 0.8 ± 0.171
2.0TyrArg: 2.0 ± 0.374
0.933TyrSer: 0.933 ± 0.239
1.378TyrThr: 1.378 ± 0.264
1.733TyrVal: 1.733 ± 0.288
0.267TyrTrp: 0.267 ± 0.115
0.533TyrTyr: 0.533 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (22503 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski