Streptococcus phage Javan110

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ATD4|A0A4D6ATD4_9CAUD Transcriptional regulator OS=Streptococcus phage Javan110 OX=2547981 GN=Javan110_0027 PE=4 SV=1
MM1 pKa = 7.78SIEE4 pKa = 4.02SSIAEE9 pKa = 4.28MRR11 pKa = 11.84CLAAIPVHH19 pKa = 7.25YY20 pKa = 10.98DD21 pKa = 2.61MGDD24 pKa = 3.2RR25 pKa = 11.84YY26 pKa = 11.26GNDD29 pKa = 3.18ADD31 pKa = 4.18RR32 pKa = 11.84DD33 pKa = 3.77GRR35 pKa = 11.84VEE37 pKa = 4.12YY38 pKa = 10.27DD39 pKa = 3.29CSSAVSKK46 pKa = 11.08AVGLSLNNNTEE57 pKa = 4.33TLKK60 pKa = 11.34SNLPTIGYY68 pKa = 8.34NCVYY72 pKa = 10.54DD73 pKa = 4.22GVDD76 pKa = 3.15GSFDD80 pKa = 3.57AQVGDD85 pKa = 3.82VVIWGPRR92 pKa = 11.84DD93 pKa = 3.39MSSSLGAFGHH103 pKa = 4.94VVIMTSPTTMIHH115 pKa = 6.36CNYY118 pKa = 9.79GYY120 pKa = 10.92DD121 pKa = 3.41GVTEE125 pKa = 3.95NDD127 pKa = 3.45YY128 pKa = 10.97NQIWEE133 pKa = 4.12INGRR137 pKa = 11.84PRR139 pKa = 11.84EE140 pKa = 3.96TVYY143 pKa = 11.04RR144 pKa = 11.84LNAQAQAAPAPQPQASNRR162 pKa = 11.84LKK164 pKa = 10.53VYY166 pKa = 9.88QVNDD170 pKa = 3.5LQFVNGIWQVKK181 pKa = 10.0CDD183 pKa = 4.25DD184 pKa = 4.13LCPVEE189 pKa = 5.99FDD191 pKa = 2.98WTQNGIACEE200 pKa = 4.86DD201 pKa = 3.53IDD203 pKa = 4.38ITDD206 pKa = 3.59ANGNVISDD214 pKa = 3.78QEE216 pKa = 4.61TKK218 pKa = 10.04VGSYY222 pKa = 9.12FVINPDD228 pKa = 3.28KK229 pKa = 11.1VVSDD233 pKa = 3.61GLGAYY238 pKa = 10.04GSGGYY243 pKa = 7.41YY244 pKa = 8.55WRR246 pKa = 11.84HH247 pKa = 3.77VTLAASGQIWLSVWDD262 pKa = 4.34VNHH265 pKa = 7.12LLYY268 pKa = 11.02GG269 pKa = 3.89

Molecular weight: 29.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ATH8|A0A4D6ATH8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan110 OX=2547981 GN=Javan110_0043 PE=4 SV=1
MM1 pKa = 7.54ISKK4 pKa = 9.44KK5 pKa = 10.2QYY7 pKa = 8.34MVSWCITTLIYY18 pKa = 10.53LIIGGTFAFWNRR30 pKa = 11.84NLRR33 pKa = 11.84IEE35 pKa = 4.17NKK37 pKa = 9.56RR38 pKa = 11.84LKK40 pKa = 9.39EE41 pKa = 3.92NQVIIYY47 pKa = 10.13RR48 pKa = 11.84VDD50 pKa = 3.12NAGAAMIGKK59 pKa = 8.26ITEE62 pKa = 3.97KK63 pKa = 10.69KK64 pKa = 10.02VIQGHH69 pKa = 4.42YY70 pKa = 8.57TVTAGAYY77 pKa = 9.73GKK79 pKa = 10.45FLVTEE84 pKa = 3.78EE85 pKa = 4.8HH86 pKa = 5.36YY87 pKa = 11.18QKK89 pKa = 10.64IKK91 pKa = 11.03VGDD94 pKa = 4.83DD95 pKa = 2.82IPEE98 pKa = 4.0FLKK101 pKa = 11.11GRR103 pKa = 11.84GNN105 pKa = 3.46

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

9451

43

1022

192.9

22.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.359 ± 0.537

0.603 ± 0.13

6.158 ± 0.376

7.692 ± 0.516

4.433 ± 0.301

5.756 ± 0.351

1.566 ± 0.19

7.671 ± 0.387

8.867 ± 0.406

8.2 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.231

6.105 ± 0.321

2.846 ± 0.198

3.851 ± 0.187

4.296 ± 0.507

5.819 ± 0.346

5.798 ± 0.439

6.095 ± 0.304

1.259 ± 0.168

3.83 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski