Amino acid dipepetide frequency for Halobiforma lacisalsi AJ5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.022AlaAla: 13.022 ± 0.161	0.685AlaCys: 0.685 ± 0.025	10.317AlaAsp: 10.317 ± 0.126	8.871AlaGlu: 8.871 ± 0.1	3.868AlaPhe: 3.868 ± 0.063	9.269AlaGly: 9.269 ± 0.107	1.648AlaHis: 1.648 ± 0.034	5.084AlaIle: 5.084 ± 0.067	1.631AlaLys: 1.631 ± 0.039	9.894AlaLeu: 9.894 ± 0.124	1.937AlaMet: 1.937 ± 0.046	2.272AlaAsn: 2.272 ± 0.044	3.627AlaPro: 3.627 ± 0.068	1.817AlaGln: 1.817 ± 0.042	5.873AlaArg: 5.873 ± 0.065	5.154AlaSer: 5.154 ± 0.067	6.981AlaThr: 6.981 ± 0.096	10.439AlaVal: 10.439 ± 0.133	1.038AlaTrp: 1.038 ± 0.033	2.625AlaTyr: 2.625 ± 0.053	0.002AlaXaa: 0.002 ± 0.002
Cys	0.535CysAla: 0.535 ± 0.02	0.099CysCys: 0.099 ± 0.009	0.589CysAsp: 0.589 ± 0.023	0.633CysGlu: 0.633 ± 0.026	0.186CysPhe: 0.186 ± 0.015	0.838CysGly: 0.838 ± 0.035	0.197CysHis: 0.197 ± 0.014	0.247CysIle: 0.247 ± 0.015	0.121CysLys: 0.121 ± 0.01	0.59CysLeu: 0.59 ± 0.022	0.105CysMet: 0.105 ± 0.011	0.181CysAsn: 0.181 ± 0.012	0.553CysPro: 0.553 ± 0.026	0.159CysGln: 0.159 ± 0.012	0.54CysArg: 0.54 ± 0.025	0.426CysSer: 0.426 ± 0.022	0.443CysThr: 0.443 ± 0.022	0.45CysVal: 0.45 ± 0.02	0.098CysTrp: 0.098 ± 0.01	0.211CysTyr: 0.211 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	9.863AspAla: 9.863 ± 0.118	0.695AspCys: 0.695 ± 0.031	8.798AspAsp: 8.798 ± 0.118	9.103AspGlu: 9.103 ± 0.117	1.777AspPhe: 1.777 ± 0.04	9.151AspGly: 9.151 ± 0.114	1.965AspHis: 1.965 ± 0.047	2.317AspIle: 2.317 ± 0.054	0.781AspLys: 0.781 ± 0.028	7.845AspLeu: 7.845 ± 0.095	1.117AspMet: 1.117 ± 0.03	1.163AspAsn: 1.163 ± 0.034	5.244AspPro: 5.244 ± 0.087	1.622AspGln: 1.622 ± 0.037	7.687AspArg: 7.687 ± 0.085	3.82AspSer: 3.82 ± 0.061	3.791AspThr: 3.791 ± 0.06	8.186AspVal: 8.186 ± 0.097	1.021AspTrp: 1.021 ± 0.03	1.881AspTyr: 1.881 ± 0.044	0.002AspXaa: 0.002 ± 0.001
Glu	9.5GluAla: 9.5 ± 0.108	0.567GluCys: 0.567 ± 0.024	7.179GluAsp: 7.179 ± 0.106	8.517GluGlu: 8.517 ± 0.138	3.298GluPhe: 3.298 ± 0.054	6.576GluGly: 6.576 ± 0.087	2.07GluHis: 2.07 ± 0.047	4.539GluIle: 4.539 ± 0.07	1.884GluLys: 1.884 ± 0.053	7.961GluLeu: 7.961 ± 0.087	1.9GluMet: 1.9 ± 0.042	2.643GluAsn: 2.643 ± 0.055	4.958GluPro: 4.958 ± 0.089	2.602GluGln: 2.602 ± 0.054	8.806GluArg: 8.806 ± 0.115	5.795GluSer: 5.795 ± 0.074	7.297GluThr: 7.297 ± 0.088	6.159GluVal: 6.159 ± 0.08	1.132GluTrp: 1.132 ± 0.031	3.077GluTyr: 3.077 ± 0.057	0.001GluXaa: 0.001 ± 0.001
Phe	3.551PheAla: 3.551 ± 0.057	0.27PheCys: 0.27 ± 0.016	3.313PheAsp: 3.313 ± 0.049	3.226PheGlu: 3.226 ± 0.059	1.005PhePhe: 1.005 ± 0.028	3.069PheGly: 3.069 ± 0.051	0.603PheHis: 0.603 ± 0.024	0.919PheIle: 0.919 ± 0.031	0.424PheLys: 0.424 ± 0.02	2.833PheLeu: 2.833 ± 0.056	0.511PheMet: 0.511 ± 0.022	0.614PheAsn: 0.614 ± 0.022	1.344PhePro: 1.344 ± 0.032	0.704PheGln: 0.704 ± 0.026	1.822PheArg: 1.822 ± 0.042	1.556PheSer: 1.556 ± 0.035	1.786PheThr: 1.786 ± 0.042	3.317PheVal: 3.317 ± 0.064	0.391PheTrp: 0.391 ± 0.018	0.837PheTyr: 0.837 ± 0.026	0.002PheXaa: 0.002 ± 0.001
Gly	7.699GlyAla: 7.699 ± 0.112	0.72GlyCys: 0.72 ± 0.028	7.623GlyAsp: 7.623 ± 0.099	7.612GlyGlu: 7.612 ± 0.102	3.048GlyPhe: 3.048 ± 0.048	7.018GlyGly: 7.018 ± 0.112	1.64GlyHis: 1.64 ± 0.039	4.344GlyIle: 4.344 ± 0.068	1.713GlyLys: 1.713 ± 0.043	7.272GlyLeu: 7.272 ± 0.114	1.694GlyMet: 1.694 ± 0.037	1.985GlyAsn: 1.985 ± 0.048	3.517GlyPro: 3.517 ± 0.059	1.842GlyGln: 1.842 ± 0.047	5.208GlyArg: 5.208 ± 0.075	5.344GlySer: 5.344 ± 0.072	6.312GlyThr: 6.312 ± 0.071	7.257GlyVal: 7.257 ± 0.081	1.097GlyTrp: 1.097 ± 0.028	2.738GlyTyr: 2.738 ± 0.046	0.006GlyXaa: 0.006 ± 0.002
His	1.93HisAla: 1.93 ± 0.042	0.222HisCys: 0.222 ± 0.013	1.787HisAsp: 1.787 ± 0.038	1.828HisGlu: 1.828 ± 0.043	0.524HisPhe: 0.524 ± 0.022	1.942HisGly: 1.942 ± 0.04	0.472HisHis: 0.472 ± 0.021	0.549HisIle: 0.549 ± 0.021	0.255HisLys: 0.255 ± 0.015	1.647HisLeu: 1.647 ± 0.04	0.253HisMet: 0.253 ± 0.015	0.402HisAsn: 0.402 ± 0.019	1.234HisPro: 1.234 ± 0.035	0.399HisGln: 0.399 ± 0.022	1.274HisArg: 1.274 ± 0.032	0.794HisSer: 0.794 ± 0.03	0.95HisThr: 0.95 ± 0.029	1.868HisVal: 1.868 ± 0.045	0.223HisTrp: 0.223 ± 0.014	0.543HisTyr: 0.543 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	4.877IleAla: 4.877 ± 0.071	0.261IleCys: 0.261 ± 0.015	4.454IleAsp: 4.454 ± 0.065	4.461IleGlu: 4.461 ± 0.069	0.972IlePhe: 0.972 ± 0.031	3.958IleGly: 3.958 ± 0.069	0.766IleHis: 0.766 ± 0.031	1.131IleIle: 1.131 ± 0.035	0.612IleLys: 0.612 ± 0.023	2.83IleLeu: 2.83 ± 0.059	0.483IleMet: 0.483 ± 0.023	0.904IleAsn: 0.904 ± 0.029	1.879IlePro: 1.879 ± 0.039	0.954IleGln: 0.954 ± 0.029	2.446IleArg: 2.446 ± 0.043	2.016IleSer: 2.016 ± 0.041	2.319IleThr: 2.319 ± 0.051	4.146IleVal: 4.146 ± 0.067	0.324IleTrp: 0.324 ± 0.015	0.949IleTyr: 0.949 ± 0.03	0.001IleXaa: 0.001 ± 0.001
Lys	1.592LysAla: 1.592 ± 0.041	0.105LysCys: 0.105 ± 0.011	0.977LysAsp: 0.977 ± 0.031	1.248LysGlu: 1.248 ± 0.043	0.463LysPhe: 0.463 ± 0.02	1.239LysGly: 1.239 ± 0.035	0.42LysHis: 0.42 ± 0.019	0.744LysIle: 0.744 ± 0.024	0.488LysLys: 0.488 ± 0.024	1.506LysLeu: 1.506 ± 0.039	0.323LysMet: 0.323 ± 0.019	0.483LysAsn: 0.483 ± 0.022	0.854LysPro: 0.854 ± 0.029	0.565LysGln: 0.565 ± 0.021	1.565LysArg: 1.565 ± 0.039	1.004LysSer: 1.004 ± 0.033	1.201LysThr: 1.201 ± 0.033	1.034LysVal: 1.034 ± 0.03	0.198LysTrp: 0.198 ± 0.013	0.493LysTyr: 0.493 ± 0.021	0.002LysXaa: 0.002 ± 0.001
Leu	10.551LeuAla: 10.551 ± 0.118	0.62LeuCys: 0.62 ± 0.027	5.602LeuAsp: 5.602 ± 0.071	12.758LeuGlu: 12.758 ± 0.12	3.046LeuPhe: 3.046 ± 0.06	7.137LeuGly: 7.137 ± 0.089	1.362LeuHis: 1.362 ± 0.034	2.923LeuIle: 2.923 ± 0.065	1.273LeuLys: 1.273 ± 0.035	8.447LeuLeu: 8.447 ± 0.126	1.137LeuMet: 1.137 ± 0.033	1.471LeuAsn: 1.471 ± 0.038	4.062LeuPro: 4.062 ± 0.06	1.848LeuGln: 1.848 ± 0.04	5.261LeuArg: 5.261 ± 0.07	5.223LeuSer: 5.223 ± 0.075	4.822LeuThr: 4.822 ± 0.066	7.942LeuVal: 7.942 ± 0.1	0.869LeuTrp: 0.869 ± 0.028	2.192LeuTyr: 2.192 ± 0.051	0.003LeuXaa: 0.003 ± 0.002
Met	1.768MetAla: 1.768 ± 0.04	0.108MetCys: 0.108 ± 0.009	1.149MetAsp: 1.149 ± 0.033	1.114MetGlu: 1.114 ± 0.03	0.472MetPhe: 0.472 ± 0.023	1.349MetGly: 1.349 ± 0.037	0.337MetHis: 0.337 ± 0.018	0.708MetIle: 0.708 ± 0.024	0.394MetLys: 0.394 ± 0.018	1.446MetLeu: 1.446 ± 0.037	0.284MetMet: 0.284 ± 0.015	0.551MetAsn: 0.551 ± 0.021	0.763MetPro: 0.763 ± 0.025	0.445MetGln: 0.445 ± 0.02	0.995MetArg: 0.995 ± 0.03	1.373MetSer: 1.373 ± 0.032	1.537MetThr: 1.537 ± 0.035	1.194MetVal: 1.194 ± 0.032	0.157MetTrp: 0.157 ± 0.012	0.395MetTyr: 0.395 ± 0.017	0.001MetXaa: 0.001 ± 0.001
Asn	2.509AsnAla: 2.509 ± 0.049	0.21AsnCys: 0.21 ± 0.014	1.718AsnAsp: 1.718 ± 0.042	1.896AsnGlu: 1.896 ± 0.044	0.577AsnPhe: 0.577 ± 0.022	2.06AsnGly: 2.06 ± 0.052	0.469AsnHis: 0.469 ± 0.021	0.713AsnIle: 0.713 ± 0.027	0.371AsnLys: 0.371 ± 0.019	1.789AsnLeu: 1.789 ± 0.039	0.363AsnMet: 0.363 ± 0.017	0.412AsnAsn: 0.412 ± 0.02	1.425AsnPro: 1.425 ± 0.038	0.525AsnGln: 0.525 ± 0.024	1.656AsnArg: 1.656 ± 0.034	0.832AsnSer: 0.832 ± 0.025	1.119AsnThr: 1.119 ± 0.034	2.18AsnVal: 2.18 ± 0.044	0.323AsnTrp: 0.323 ± 0.018	0.616AsnTyr: 0.616 ± 0.023	0.001AsnXaa: 0.001 ± 0.001
Pro	4.734ProAla: 4.734 ± 0.07	0.253ProCys: 0.253 ± 0.015	5.308ProAsp: 5.308 ± 0.079	5.026ProGlu: 5.026 ± 0.078	1.591ProPhe: 1.591 ± 0.041	3.99ProGly: 3.99 ± 0.064	0.844ProHis: 0.844 ± 0.027	2.131ProIle: 2.131 ± 0.043	0.751ProLys: 0.751 ± 0.026	3.82ProLeu: 3.82 ± 0.058	0.831ProMet: 0.831 ± 0.028	1.161ProAsn: 1.161 ± 0.029	2.143ProPro: 2.143 ± 0.05	0.897ProGln: 0.897 ± 0.031	2.423ProArg: 2.423 ± 0.046	2.663ProSer: 2.663 ± 0.051	3.138ProThr: 3.138 ± 0.064	4.122ProVal: 4.122 ± 0.06	0.524ProTrp: 0.524 ± 0.022	1.08ProTyr: 1.08 ± 0.029	0.002ProXaa: 0.002 ± 0.001
Gln	2.039GlnAla: 2.039 ± 0.039	0.151GlnCys: 0.151 ± 0.01	1.314GlnAsp: 1.314 ± 0.036	1.829GlnGlu: 1.829 ± 0.051	0.929GlnPhe: 0.929 ± 0.024	1.552GlnGly: 1.552 ± 0.042	0.464GlnHis: 0.464 ± 0.019	0.921GlnIle: 0.921 ± 0.025	0.467GlnLys: 0.467 ± 0.021	2.174GlnLeu: 2.174 ± 0.042	0.426GlnMet: 0.426 ± 0.019	0.582GlnAsn: 0.582 ± 0.025	1.104GlnPro: 1.104 ± 0.034	0.882GlnGln: 0.882 ± 0.035	1.906GlnArg: 1.906 ± 0.044	1.359GlnSer: 1.359 ± 0.033	1.28GlnThr: 1.28 ± 0.034	1.737GlnVal: 1.737 ± 0.033	0.313GlnTrp: 0.313 ± 0.018	0.757GlnTyr: 0.757 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	6.206ArgAla: 6.206 ± 0.071	0.499ArgCys: 0.499 ± 0.025	5.267ArgAsp: 5.267 ± 0.083	7.374ArgGlu: 7.374 ± 0.088	2.32ArgPhe: 2.32 ± 0.038	4.547ArgGly: 4.547 ± 0.058	1.211ArgHis: 1.211 ± 0.03	3.47ArgIle: 3.47 ± 0.054	1.433ArgLys: 1.433 ± 0.038	6.402ArgLeu: 6.402 ± 0.079	1.288ArgMet: 1.288 ± 0.034	1.679ArgAsn: 1.679 ± 0.042	2.788ArgPro: 2.788 ± 0.054	1.702ArgGln: 1.702 ± 0.041	5.49ArgArg: 5.49 ± 0.083	4.057ArgSer: 4.057 ± 0.064	4.43ArgThr: 4.43 ± 0.07	5.049ArgVal: 5.049 ± 0.068	0.795ArgTrp: 0.795 ± 0.03	1.97ArgTyr: 1.97 ± 0.046	0.005ArgXaa: 0.005 ± 0.002
Ser	5.065SerAla: 5.065 ± 0.076	0.332SerCys: 0.332 ± 0.018	4.714SerAsp: 4.714 ± 0.069	4.549SerGlu: 4.549 ± 0.071	1.775SerPhe: 1.775 ± 0.035	5.181SerGly: 5.181 ± 0.068	0.982SerHis: 0.982 ± 0.025	2.562SerIle: 2.562 ± 0.047	1.074SerLys: 1.074 ± 0.033	4.688SerLeu: 4.688 ± 0.063	1.073SerMet: 1.073 ± 0.033	1.302SerAsn: 1.302 ± 0.034	2.642SerPro: 2.642 ± 0.053	1.261SerGln: 1.261 ± 0.033	3.416SerArg: 3.416 ± 0.052	3.383SerSer: 3.383 ± 0.069	3.421SerThr: 3.421 ± 0.055	4.697SerVal: 4.697 ± 0.065	0.608SerTrp: 0.608 ± 0.025	1.428SerTyr: 1.428 ± 0.034	0.002SerXaa: 0.002 ± 0.001
Thr	6.823ThrAla: 6.823 ± 0.086	0.401ThrCys: 0.401 ± 0.018	5.95ThrAsp: 5.95 ± 0.081	5.027ThrGlu: 5.027 ± 0.076	2.063ThrPhe: 2.063 ± 0.044	5.886ThrGly: 5.886 ± 0.081	1.11ThrHis: 1.11 ± 0.03	3.167ThrIle: 3.167 ± 0.06	0.96ThrLys: 0.96 ± 0.027	5.599ThrLeu: 5.599 ± 0.067	1.015ThrMet: 1.015 ± 0.03	1.407ThrAsn: 1.407 ± 0.038	3.074ThrPro: 3.074 ± 0.052	1.222ThrGln: 1.222 ± 0.036	3.21ThrArg: 3.21 ± 0.05	2.764ThrSer: 2.764 ± 0.056	4.056ThrThr: 4.056 ± 0.068	6.921ThrVal: 6.921 ± 0.095	0.612ThrTrp: 0.612 ± 0.026	1.681ThrTyr: 1.681 ± 0.038	0.001ThrXaa: 0.001 ± 0.001
Val	10.137ValAla: 10.137 ± 0.135	0.645ValCys: 0.645 ± 0.025	8.199ValAsp: 8.199 ± 0.097	7.814ValGlu: 7.814 ± 0.093	2.856ValPhe: 2.856 ± 0.06	7.741ValGly: 7.741 ± 0.106	1.659ValHis: 1.659 ± 0.042	3.059ValIle: 3.059 ± 0.058	1.233ValLys: 1.233 ± 0.032	8.117ValLeu: 8.117 ± 0.091	1.246ValMet: 1.246 ± 0.031	1.68ValAsn: 1.68 ± 0.038	4.508ValPro: 4.508 ± 0.064	1.784ValGln: 1.784 ± 0.037	5.526ValArg: 5.526 ± 0.08	4.867ValSer: 4.867 ± 0.062	5.889ValThr: 5.889 ± 0.084	8.909ValVal: 8.909 ± 0.119	0.832ValTrp: 0.832 ± 0.027	2.109ValTyr: 2.109 ± 0.043	0.002ValXaa: 0.002 ± 0.001
Trp	0.9TrpAla: 0.9 ± 0.032	0.094TrpCys: 0.094 ± 0.009	0.768TrpAsp: 0.768 ± 0.027	0.886TrpGlu: 0.886 ± 0.031	0.46TrpPhe: 0.46 ± 0.022	0.793TrpGly: 0.793 ± 0.027	0.26TrpHis: 0.26 ± 0.015	0.558TrpIle: 0.558 ± 0.02	0.25TrpLys: 0.25 ± 0.013	1.235TrpLeu: 1.235 ± 0.035	0.204TrpMet: 0.204 ± 0.012	0.35TrpAsn: 0.35 ± 0.018	0.447TrpPro: 0.447 ± 0.022	0.323TrpGln: 0.323 ± 0.016	0.83TrpArg: 0.83 ± 0.03	0.586TrpSer: 0.586 ± 0.022	0.799TrpThr: 0.799 ± 0.027	0.785TrpVal: 0.785 ± 0.026	0.219TrpTrp: 0.219 ± 0.016	0.37TrpTyr: 0.37 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.748TyrAla: 2.748 ± 0.048	0.264TyrCys: 0.264 ± 0.016	2.802TyrAsp: 2.802 ± 0.049	2.685TyrGlu: 2.685 ± 0.055	0.868TyrPhe: 0.868 ± 0.026	2.392TyrGly: 2.392 ± 0.048	0.619TyrHis: 0.619 ± 0.021	0.656TyrIle: 0.656 ± 0.024	0.385TyrLys: 0.385 ± 0.017	2.45TyrLeu: 2.45 ± 0.053	0.347TyrMet: 0.347 ± 0.016	0.522TyrAsn: 0.522 ± 0.021	1.26TyrPro: 1.26 ± 0.034	0.632TyrGln: 0.632 ± 0.024	2.048TyrArg: 2.048 ± 0.042	1.137TyrSer: 1.137 ± 0.028	1.379TyrThr: 1.379 ± 0.035	2.417TyrVal: 2.417 ± 0.047	0.341TyrTrp: 0.341 ± 0.02	0.862TyrTyr: 0.862 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.004XaaGlu: 0.004 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.002XaaLys: 0.002 ± 0.001	0.003XaaLeu: 0.003 ± 0.002	0.002XaaMet: 0.002 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.004XaaPro: 0.004 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4151 proteins (1212350 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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