Velvet tobacco mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2IZN1|E2IZN1_9VIRU N-terminal protein OS=Velvet tobacco mottle virus OX=12473 PE=4 SV=1
MM1 pKa = 7.71PSIDD5 pKa = 3.78VEE7 pKa = 4.43VEE9 pKa = 4.3KK10 pKa = 10.64ILHH13 pKa = 5.77LSNRR17 pKa = 11.84KK18 pKa = 8.79RR19 pKa = 11.84LRR21 pKa = 11.84SVCVARR27 pKa = 11.84KK28 pKa = 8.33KK29 pKa = 9.37TFVDD33 pKa = 4.34KK34 pKa = 10.72ISEE37 pKa = 4.32GFVCTLYY44 pKa = 11.2GEE46 pKa = 4.41YY47 pKa = 10.77DD48 pKa = 3.29HH49 pKa = 7.6DD50 pKa = 4.56YY51 pKa = 8.2VTSVHH56 pKa = 5.63LHH58 pKa = 5.87IVCSCGRR65 pKa = 11.84AFFDD69 pKa = 3.88FVEE72 pKa = 5.1FKK74 pKa = 10.59DD75 pKa = 3.18IKK77 pKa = 11.01LRR79 pKa = 11.84DD80 pKa = 3.68FQYY83 pKa = 10.89QSQCICPTRR92 pKa = 11.84NWSTVAYY99 pKa = 9.68TDD101 pKa = 3.91FTTVNCQVEE110 pKa = 4.48GCDD113 pKa = 3.33YY114 pKa = 11.44CEE116 pKa = 5.1GIEE119 pKa = 4.43SDD121 pKa = 3.74SDD123 pKa = 3.78SDD125 pKa = 3.89SEE127 pKa = 4.73AIIEE131 pKa = 4.1EE132 pKa = 4.18FLQKK136 pKa = 10.43FSEE139 pKa = 4.31VGISGSSSSPQTNN152 pKa = 2.6

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2IZN3|E2IZN3_9VIRU Capsid protein OS=Velvet tobacco mottle virus OX=12473 GN=CP PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.89KK4 pKa = 8.99LTKK7 pKa = 10.61NQVKK11 pKa = 10.2QMIQATLPKK20 pKa = 9.12EE21 pKa = 3.99QTSARR26 pKa = 11.84SRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84STQQGQSSTVMAPMAGAVIYY54 pKa = 9.99RR55 pKa = 11.84KK56 pKa = 9.99RR57 pKa = 11.84PMLINGRR64 pKa = 11.84SGVTVRR70 pKa = 11.84HH71 pKa = 5.6SEE73 pKa = 4.14VVLVVQSGTTNFSATSVTLAPNTFTWLAVQGSLYY107 pKa = 10.9SKK109 pKa = 9.65WRR111 pKa = 11.84WISLRR116 pKa = 11.84ATYY119 pKa = 10.55VPEE122 pKa = 4.34TASTTPGTVAMGFQYY137 pKa = 11.3DD138 pKa = 3.87NTDD141 pKa = 3.32VLPTGTAGMSSLHH154 pKa = 6.12GFVSGAPWSGFSGSKK169 pKa = 10.34LLAEE173 pKa = 4.93SPTTPIPAGAIATRR187 pKa = 11.84LDD189 pKa = 3.7CQNFGLKK196 pKa = 8.85WYY198 pKa = 9.3QYY200 pKa = 10.84KK201 pKa = 10.45SVIPAGDD208 pKa = 3.31SGNIYY213 pKa = 10.21IPAQLIVGTLGTGSTLRR230 pKa = 11.84YY231 pKa = 10.0GEE233 pKa = 3.94VHH235 pKa = 5.53IQYY238 pKa = 9.46EE239 pKa = 4.2IEE241 pKa = 4.77FIEE244 pKa = 4.24PLPPSVNTLRR254 pKa = 11.84DD255 pKa = 3.04IYY257 pKa = 11.42SRR259 pKa = 11.84MILSSASDD267 pKa = 3.34EE268 pKa = 4.36RR269 pKa = 11.84KK270 pKa = 10.29GEE272 pKa = 4.08KK273 pKa = 9.92SSQTLTDD280 pKa = 3.22RR281 pKa = 4.51

Molecular weight: 30.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1553

152

572

388.3

43.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.504 ± 0.681

2.318 ± 0.88

4.701 ± 0.768

6.89 ± 0.875

3.67 ± 0.598

6.439 ± 0.449

1.674 ± 0.243

4.25 ± 0.574

5.666 ± 0.631

8.693 ± 0.836

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.338

3.348 ± 0.287

5.473 ± 0.712

3.67 ± 0.387

6.182 ± 0.559

11.011 ± 0.602

5.409 ± 1.107

6.954 ± 1.14

1.996 ± 0.436

2.962 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski