Mycobacterium phage SuperGrey

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GPM9|A0A1J0GPM9_9CAUD Uncharacterized protein OS=Mycobacterium phage SuperGrey OX=1913038 GN=SEA_SUPERGREY_34 PE=4 SV=1
MM1 pKa = 7.24MSADD5 pKa = 3.89PVRR8 pKa = 11.84GAIQASLDD16 pKa = 3.5AMGDD20 pKa = 3.44GWQVAHH26 pKa = 5.73YY27 pKa = 9.08VVVVGLEE34 pKa = 4.17RR35 pKa = 11.84IDD37 pKa = 4.1GDD39 pKa = 4.67RR40 pKa = 11.84MDD42 pKa = 5.5LGATTVITPIGQAGYY57 pKa = 8.35VTDD60 pKa = 4.2GLVNRR65 pKa = 11.84YY66 pKa = 7.35WDD68 pKa = 3.73EE69 pKa = 4.28SSGEE73 pKa = 3.97

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GPT8|A0A1J0GPT8_9CAUD Uncharacterized protein OS=Mycobacterium phage SuperGrey OX=1913038 GN=SEA_SUPERGREY_97 PE=4 SV=1
MM1 pKa = 7.26VHH3 pKa = 6.58ISGTIFGMSSYY14 pKa = 10.09RR15 pKa = 11.84VEE17 pKa = 4.38IEE19 pKa = 3.64TSAAKK24 pKa = 9.98QIQRR28 pKa = 11.84LQRR31 pKa = 11.84SEE33 pKa = 3.93QKK35 pKa = 10.42RR36 pKa = 11.84VMVAITALADD46 pKa = 3.41DD47 pKa = 5.03PRR49 pKa = 11.84PHH51 pKa = 7.07GCTKK55 pKa = 10.92LSGTTDD61 pKa = 3.25AYY63 pKa = 10.48RR64 pKa = 11.84IRR66 pKa = 11.84VGNFRR71 pKa = 11.84VVYY74 pKa = 10.62VIDD77 pKa = 5.16DD78 pKa = 3.77GLHH81 pKa = 5.33IVNVTRR87 pKa = 11.84VGHH90 pKa = 5.57RR91 pKa = 11.84RR92 pKa = 11.84EE93 pKa = 4.0VYY95 pKa = 10.06KK96 pKa = 10.83RR97 pKa = 3.3

Molecular weight: 11.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

19206

30

1215

186.5

20.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.648 ± 0.405

1.182 ± 0.159

6.534 ± 0.227

5.967 ± 0.363

3.004 ± 0.185

8.81 ± 0.742

2.343 ± 0.175

4.353 ± 0.209

3.327 ± 0.184

7.394 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.123

3.223 ± 0.18

5.915 ± 0.219

3.468 ± 0.195

6.935 ± 0.412

5.951 ± 0.245

6.571 ± 0.258

7.315 ± 0.241

2.374 ± 0.153

2.416 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski