Anoxybacillus vitaminiphilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anoxybacillus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327YG94|A0A327YG94_9BACI Ribonuclease BN (tRNA processing enzyme) OS=Anoxybacillus vitaminiphilus OX=581036 GN=B0I26_106125 PE=4 SV=1
MM1 pKa = 8.01ADD3 pKa = 3.16NVLDD7 pKa = 3.89VVVDD11 pKa = 3.85SVLDD15 pKa = 3.75VVVDD19 pKa = 3.98SVLDD23 pKa = 3.75AVVDD27 pKa = 4.15SVLDD31 pKa = 3.86SVSDD35 pKa = 3.73NVLDD39 pKa = 4.11VVLTALAASIAGVVVLEE56 pKa = 4.45EE57 pKa = 4.36VSSSLAQKK65 pKa = 10.8GDD67 pKa = 3.58YY68 pKa = 10.2LRR70 pKa = 11.84RR71 pKa = 11.84DD72 pKa = 4.21CPCIICCRR80 pKa = 11.84GPLYY84 pKa = 11.01KK85 pKa = 10.65NN86 pKa = 3.38

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327Y6W1|A0A327Y6W1_9BACI Restriction endonuclease OS=Anoxybacillus vitaminiphilus OX=581036 GN=B0I26_11524 PE=4 SV=1
RR1 pKa = 8.17VWIPKK6 pKa = 9.46QRR8 pKa = 11.84TSFLAHH14 pKa = 6.82PPRR17 pKa = 11.84NGRR20 pKa = 11.84EE21 pKa = 3.66ICHH24 pKa = 6.31PRR26 pKa = 11.84LLVMNQYY33 pKa = 10.72LYY35 pKa = 11.12SILQKK40 pKa = 9.91HH41 pKa = 5.04QKK43 pKa = 9.53SRR45 pKa = 11.84PSFSTTLLSFLYY57 pKa = 9.98IRR59 pKa = 11.84EE60 pKa = 3.89KK61 pKa = 10.69RR62 pKa = 11.84AILAPFCFKK71 pKa = 10.61CDD73 pKa = 3.42NKK75 pKa = 10.44LWSYY79 pKa = 10.31HH80 pKa = 6.54KK81 pKa = 10.09PPCHH85 pKa = 6.32QKK87 pKa = 10.62RR88 pKa = 11.84EE89 pKa = 4.16FLLTMKK95 pKa = 7.73TTPSNPQIIPKK106 pKa = 9.22PDD108 pKa = 3.4SPNVVPSNPSEE119 pKa = 4.02SRR121 pKa = 11.84LGKK124 pKa = 10.1RR125 pKa = 11.84SPNMVSRR132 pKa = 11.84FVQNIDD138 pKa = 2.99RR139 pKa = 4.5

Molecular weight: 16.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3513

0

3513

988767

26

2035

281.5

31.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.238 ± 0.046

0.777 ± 0.012

4.689 ± 0.031

7.919 ± 0.049

4.535 ± 0.035

6.569 ± 0.042

2.222 ± 0.021

7.806 ± 0.038

7.282 ± 0.041

9.787 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.021

4.109 ± 0.029

3.675 ± 0.025

3.944 ± 0.03

4.453 ± 0.031

5.323 ± 0.029

5.153 ± 0.03

7.096 ± 0.038

1.015 ± 0.015

3.661 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski