Amino acid dipepetide frequency for Mycobacterium phage Sumter

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.949AlaAla: 11.949 ± 1.143
0.678AlaCys: 0.678 ± 0.203
6.221AlaAsp: 6.221 ± 0.529
6.344AlaGlu: 6.344 ± 0.752
3.141AlaPhe: 3.141 ± 0.472
8.315AlaGly: 8.315 ± 1.044
1.601AlaHis: 1.601 ± 0.386
3.881AlaIle: 3.881 ± 0.545
4.312AlaLys: 4.312 ± 0.554
8.685AlaLeu: 8.685 ± 0.921
2.587AlaMet: 2.587 ± 0.383
2.587AlaAsn: 2.587 ± 0.399
4.681AlaPro: 4.681 ± 0.712
2.833AlaGln: 2.833 ± 0.499
5.913AlaArg: 5.913 ± 0.528
4.62AlaSer: 4.62 ± 0.498
6.591AlaThr: 6.591 ± 0.613
8.254AlaVal: 8.254 ± 0.76
1.54AlaTrp: 1.54 ± 0.327
2.464AlaTyr: 2.464 ± 0.342
0.0AlaXaa: 0.0 ± 0.0
Cys
0.678CysAla: 0.678 ± 0.215
0.062CysCys: 0.062 ± 0.048
0.678CysAsp: 0.678 ± 0.206
0.554CysGlu: 0.554 ± 0.171
0.185CysPhe: 0.185 ± 0.104
0.616CysGly: 0.616 ± 0.204
0.185CysHis: 0.185 ± 0.081
0.185CysIle: 0.185 ± 0.113
0.308CysLys: 0.308 ± 0.132
0.493CysLeu: 0.493 ± 0.172
0.123CysMet: 0.123 ± 0.088
0.37CysAsn: 0.37 ± 0.142
0.308CysPro: 0.308 ± 0.137
0.246CysGln: 0.246 ± 0.118
0.308CysArg: 0.308 ± 0.117
0.616CysSer: 0.616 ± 0.173
0.308CysThr: 0.308 ± 0.115
0.246CysVal: 0.246 ± 0.123
0.123CysTrp: 0.123 ± 0.078
0.308CysTyr: 0.308 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
6.344AspAla: 6.344 ± 0.597
0.739AspCys: 0.739 ± 0.225
5.174AspAsp: 5.174 ± 0.491
3.819AspGlu: 3.819 ± 0.512
2.033AspPhe: 2.033 ± 0.324
6.283AspGly: 6.283 ± 0.674
1.109AspHis: 1.109 ± 0.26
2.649AspIle: 2.649 ± 0.441
2.587AspLys: 2.587 ± 0.396
6.96AspLeu: 6.96 ± 0.81
1.109AspMet: 1.109 ± 0.252
1.971AspAsn: 1.971 ± 0.315
4.866AspPro: 4.866 ± 0.593
1.478AspGln: 1.478 ± 0.34
4.25AspArg: 4.25 ± 0.488
2.895AspSer: 2.895 ± 0.412
3.942AspThr: 3.942 ± 0.444
4.62AspVal: 4.62 ± 0.503
1.848AspTrp: 1.848 ± 0.356
2.217AspTyr: 2.217 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
6.344GluAla: 6.344 ± 0.759
0.37GluCys: 0.37 ± 0.166
5.297GluAsp: 5.297 ± 0.572
5.112GluGlu: 5.112 ± 0.567
2.094GluPhe: 2.094 ± 0.374
4.25GluGly: 4.25 ± 0.536
1.294GluHis: 1.294 ± 0.326
3.634GluIle: 3.634 ± 0.524
2.71GluLys: 2.71 ± 0.412
6.714GluLeu: 6.714 ± 0.65
1.54GluMet: 1.54 ± 0.282
1.725GluAsn: 1.725 ± 0.406
2.525GluPro: 2.525 ± 0.388
2.833GluGln: 2.833 ± 0.411
3.634GluArg: 3.634 ± 0.611
3.573GluSer: 3.573 ± 0.44
4.127GluThr: 4.127 ± 0.551
5.297GluVal: 5.297 ± 0.718
1.478GluTrp: 1.478 ± 0.319
2.464GluTyr: 2.464 ± 0.474
0.0GluXaa: 0.0 ± 0.0
Phe
2.402PheAla: 2.402 ± 0.389
0.37PheCys: 0.37 ± 0.19
2.525PheAsp: 2.525 ± 0.372
2.033PheGlu: 2.033 ± 0.342
0.739PhePhe: 0.739 ± 0.204
3.511PheGly: 3.511 ± 0.521
0.493PheHis: 0.493 ± 0.195
1.478PheIle: 1.478 ± 0.301
1.17PheLys: 1.17 ± 0.278
2.156PheLeu: 2.156 ± 0.422
0.616PheMet: 0.616 ± 0.2
1.417PheAsn: 1.417 ± 0.339
1.54PhePro: 1.54 ± 0.305
0.801PheGln: 0.801 ± 0.18
1.848PheArg: 1.848 ± 0.373
1.786PheSer: 1.786 ± 0.279
2.156PheThr: 2.156 ± 0.33
1.971PheVal: 1.971 ± 0.378
0.554PheTrp: 0.554 ± 0.185
0.924PheTyr: 0.924 ± 0.26
0.0PheXaa: 0.0 ± 0.0
Gly
7.268GlyAla: 7.268 ± 1.099
0.678GlyCys: 0.678 ± 0.216
6.16GlyAsp: 6.16 ± 0.593
4.866GlyGlu: 4.866 ± 0.494
2.71GlyPhe: 2.71 ± 0.518
9.917GlyGly: 9.917 ± 3.253
2.033GlyHis: 2.033 ± 0.406
4.496GlyIle: 4.496 ± 0.669
3.819GlyLys: 3.819 ± 0.525
8.069GlyLeu: 8.069 ± 0.771
1.971GlyMet: 1.971 ± 0.333
3.511GlyAsn: 3.511 ± 0.465
4.004GlyPro: 4.004 ± 0.554
2.464GlyGln: 2.464 ± 0.411
5.112GlyArg: 5.112 ± 0.669
6.406GlySer: 6.406 ± 1.194
5.051GlyThr: 5.051 ± 0.739
5.544GlyVal: 5.544 ± 0.661
2.71GlyTrp: 2.71 ± 0.38
2.833GlyTyr: 2.833 ± 0.45
0.0GlyXaa: 0.0 ± 0.0
His
1.478HisAla: 1.478 ± 0.31
0.062HisCys: 0.062 ± 0.056
1.232HisAsp: 1.232 ± 0.239
1.601HisGlu: 1.601 ± 0.367
0.554HisPhe: 0.554 ± 0.207
1.478HisGly: 1.478 ± 0.345
0.616HisHis: 0.616 ± 0.22
0.739HisIle: 0.739 ± 0.187
0.986HisLys: 0.986 ± 0.302
1.478HisLeu: 1.478 ± 0.337
0.062HisMet: 0.062 ± 0.065
0.246HisAsn: 0.246 ± 0.117
1.294HisPro: 1.294 ± 0.262
0.924HisGln: 0.924 ± 0.228
1.417HisArg: 1.417 ± 0.332
0.554HisSer: 0.554 ± 0.17
0.924HisThr: 0.924 ± 0.248
1.601HisVal: 1.601 ± 0.322
0.493HisTrp: 0.493 ± 0.161
0.739HisTyr: 0.739 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
6.036IleAla: 6.036 ± 0.478
0.246IleCys: 0.246 ± 0.116
3.634IleAsp: 3.634 ± 0.459
3.634IleGlu: 3.634 ± 0.455
0.801IlePhe: 0.801 ± 0.206
3.819IleGly: 3.819 ± 0.515
0.924IleHis: 0.924 ± 0.261
1.725IleIle: 1.725 ± 0.306
1.848IleLys: 1.848 ± 0.367
3.141IleLeu: 3.141 ± 0.436
0.801IleMet: 0.801 ± 0.201
1.971IleAsn: 1.971 ± 0.327
2.957IlePro: 2.957 ± 0.426
1.232IleGln: 1.232 ± 0.256
3.203IleArg: 3.203 ± 0.502
3.326IleSer: 3.326 ± 0.423
3.757IleThr: 3.757 ± 0.472
2.833IleVal: 2.833 ± 0.553
0.739IleTrp: 0.739 ± 0.216
1.848IleTyr: 1.848 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
3.388LysAla: 3.388 ± 0.479
0.185LysCys: 0.185 ± 0.108
2.279LysAsp: 2.279 ± 0.445
2.341LysGlu: 2.341 ± 0.423
1.663LysPhe: 1.663 ± 0.271
2.402LysGly: 2.402 ± 0.388
0.986LysHis: 0.986 ± 0.215
2.402LysIle: 2.402 ± 0.432
2.094LysLys: 2.094 ± 0.483
3.573LysLeu: 3.573 ± 0.498
1.294LysMet: 1.294 ± 0.239
1.663LysAsn: 1.663 ± 0.309
2.71LysPro: 2.71 ± 0.46
1.725LysGln: 1.725 ± 0.386
3.265LysArg: 3.265 ± 0.488
2.587LysSer: 2.587 ± 0.422
2.525LysThr: 2.525 ± 0.481
3.388LysVal: 3.388 ± 0.538
0.801LysTrp: 0.801 ± 0.218
1.232LysTyr: 1.232 ± 0.339
0.0LysXaa: 0.0 ± 0.0
Leu
9.178LeuAla: 9.178 ± 0.677
0.246LeuCys: 0.246 ± 0.115
6.344LeuAsp: 6.344 ± 0.556
5.667LeuGlu: 5.667 ± 0.618
2.464LeuPhe: 2.464 ± 0.419
7.576LeuGly: 7.576 ± 0.721
1.417LeuHis: 1.417 ± 0.303
4.188LeuIle: 4.188 ± 0.599
4.188LeuLys: 4.188 ± 0.568
6.468LeuLeu: 6.468 ± 0.705
1.54LeuMet: 1.54 ± 0.264
2.649LeuAsn: 2.649 ± 0.446
5.42LeuPro: 5.42 ± 0.557
2.833LeuGln: 2.833 ± 0.552
6.406LeuArg: 6.406 ± 0.58
6.098LeuSer: 6.098 ± 0.464
5.852LeuThr: 5.852 ± 0.445
4.496LeuVal: 4.496 ± 0.673
0.862LeuTrp: 0.862 ± 0.285
2.402LeuTyr: 2.402 ± 0.455
0.0LeuXaa: 0.0 ± 0.0
Met
2.772MetAla: 2.772 ± 0.411
0.0MetCys: 0.0 ± 0.0
0.986MetAsp: 0.986 ± 0.22
1.601MetGlu: 1.601 ± 0.288
0.493MetPhe: 0.493 ± 0.15
1.355MetGly: 1.355 ± 0.302
0.185MetHis: 0.185 ± 0.108
0.616MetIle: 0.616 ± 0.224
1.232MetLys: 1.232 ± 0.257
1.232MetLeu: 1.232 ± 0.288
0.062MetMet: 0.062 ± 0.052
1.109MetAsn: 1.109 ± 0.261
0.862MetPro: 0.862 ± 0.234
0.554MetGln: 0.554 ± 0.173
1.478MetArg: 1.478 ± 0.319
2.341MetSer: 2.341 ± 0.414
1.909MetThr: 1.909 ± 0.282
0.986MetVal: 0.986 ± 0.234
0.308MetTrp: 0.308 ± 0.129
0.37MetTyr: 0.37 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.388AsnAla: 3.388 ± 0.523
0.062AsnCys: 0.062 ± 0.058
1.909AsnAsp: 1.909 ± 0.393
1.971AsnGlu: 1.971 ± 0.346
0.862AsnPhe: 0.862 ± 0.222
4.312AsnGly: 4.312 ± 0.973
0.678AsnHis: 0.678 ± 0.203
1.725AsnIle: 1.725 ± 0.311
0.739AsnLys: 0.739 ± 0.218
2.464AsnLeu: 2.464 ± 0.35
0.616AsnMet: 0.616 ± 0.153
0.801AsnAsn: 0.801 ± 0.187
2.772AsnPro: 2.772 ± 0.355
1.294AsnGln: 1.294 ± 0.375
1.478AsnArg: 1.478 ± 0.363
1.971AsnSer: 1.971 ± 0.448
1.909AsnThr: 1.909 ± 0.341
2.525AsnVal: 2.525 ± 0.398
0.801AsnTrp: 0.801 ± 0.197
1.17AsnTyr: 1.17 ± 0.271
0.0AsnXaa: 0.0 ± 0.0
Pro
4.989ProAla: 4.989 ± 0.544
0.308ProCys: 0.308 ± 0.134
4.127ProAsp: 4.127 ± 0.44
4.065ProGlu: 4.065 ± 0.568
2.279ProPhe: 2.279 ± 0.393
4.866ProGly: 4.866 ± 0.593
0.678ProHis: 0.678 ± 0.196
2.402ProIle: 2.402 ± 0.372
2.094ProLys: 2.094 ± 0.321
4.065ProLeu: 4.065 ± 0.507
0.862ProMet: 0.862 ± 0.228
1.478ProAsn: 1.478 ± 0.267
2.525ProPro: 2.525 ± 0.426
1.417ProGln: 1.417 ± 0.31
2.525ProArg: 2.525 ± 0.455
3.449ProSer: 3.449 ± 0.482
3.819ProThr: 3.819 ± 0.594
4.004ProVal: 4.004 ± 0.559
0.801ProTrp: 0.801 ± 0.296
1.54ProTyr: 1.54 ± 0.384
0.0ProXaa: 0.0 ± 0.0
Gln
3.018GlnAla: 3.018 ± 0.474
0.123GlnCys: 0.123 ± 0.089
1.232GlnAsp: 1.232 ± 0.359
1.971GlnGlu: 1.971 ± 0.342
1.109GlnPhe: 1.109 ± 0.227
2.525GlnGly: 2.525 ± 0.382
0.616GlnHis: 0.616 ± 0.156
2.833GlnIle: 2.833 ± 0.487
1.17GlnLys: 1.17 ± 0.243
3.573GlnLeu: 3.573 ± 0.538
0.924GlnMet: 0.924 ± 0.266
0.493GlnAsn: 0.493 ± 0.174
1.909GlnPro: 1.909 ± 0.295
1.663GlnGln: 1.663 ± 0.331
1.786GlnArg: 1.786 ± 0.363
2.033GlnSer: 2.033 ± 0.331
1.54GlnThr: 1.54 ± 0.342
2.341GlnVal: 2.341 ± 0.312
0.554GlnTrp: 0.554 ± 0.186
0.554GlnTyr: 0.554 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
5.79ArgAla: 5.79 ± 0.587
0.924ArgCys: 0.924 ± 0.284
3.265ArgAsp: 3.265 ± 0.451
4.743ArgGlu: 4.743 ± 0.709
1.848ArgPhe: 1.848 ± 0.358
5.297ArgGly: 5.297 ± 0.746
1.047ArgHis: 1.047 ± 0.245
3.265ArgIle: 3.265 ± 0.496
3.449ArgLys: 3.449 ± 0.564
6.344ArgLeu: 6.344 ± 0.741
2.094ArgMet: 2.094 ± 0.369
2.279ArgAsn: 2.279 ± 0.466
2.033ArgPro: 2.033 ± 0.338
1.971ArgGln: 1.971 ± 0.337
5.728ArgArg: 5.728 ± 0.754
3.696ArgSer: 3.696 ± 0.462
2.71ArgThr: 2.71 ± 0.417
5.051ArgVal: 5.051 ± 0.629
1.355ArgTrp: 1.355 ± 0.334
1.725ArgTyr: 1.725 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
6.283SerAla: 6.283 ± 0.708
0.616SerCys: 0.616 ± 0.215
3.388SerAsp: 3.388 ± 0.508
4.065SerGlu: 4.065 ± 0.472
1.786SerPhe: 1.786 ± 0.388
6.899SerGly: 6.899 ± 1.444
1.355SerHis: 1.355 ± 0.269
2.957SerIle: 2.957 ± 0.443
2.525SerLys: 2.525 ± 0.437
4.989SerLeu: 4.989 ± 0.536
1.54SerMet: 1.54 ± 0.301
2.71SerAsn: 2.71 ± 0.503
2.833SerPro: 2.833 ± 0.449
1.786SerGln: 1.786 ± 0.256
3.388SerArg: 3.388 ± 0.424
3.265SerSer: 3.265 ± 0.733
2.957SerThr: 2.957 ± 0.45
3.942SerVal: 3.942 ± 0.481
1.355SerTrp: 1.355 ± 0.367
1.355SerTyr: 1.355 ± 0.282
0.0SerXaa: 0.0 ± 0.0
Thr
5.728ThrAla: 5.728 ± 0.552
0.246ThrCys: 0.246 ± 0.142
4.312ThrAsp: 4.312 ± 0.535
4.373ThrGlu: 4.373 ± 0.474
2.094ThrPhe: 2.094 ± 0.381
6.899ThrGly: 6.899 ± 0.727
0.678ThrHis: 0.678 ± 0.227
2.649ThrIle: 2.649 ± 0.582
2.464ThrLys: 2.464 ± 0.304
5.975ThrLeu: 5.975 ± 0.651
0.739ThrMet: 0.739 ± 0.186
1.725ThrAsn: 1.725 ± 0.368
3.449ThrPro: 3.449 ± 0.548
1.848ThrGln: 1.848 ± 0.317
3.265ThrArg: 3.265 ± 0.52
3.573ThrSer: 3.573 ± 0.518
4.312ThrThr: 4.312 ± 0.567
5.79ThrVal: 5.79 ± 0.641
0.986ThrTrp: 0.986 ± 0.242
1.725ThrTyr: 1.725 ± 0.374
0.0ThrXaa: 0.0 ± 0.0
Val
6.098ValAla: 6.098 ± 0.694
0.431ValCys: 0.431 ± 0.162
5.42ValAsp: 5.42 ± 0.61
5.174ValGlu: 5.174 ± 0.535
2.402ValPhe: 2.402 ± 0.391
5.051ValGly: 5.051 ± 0.66
1.54ValHis: 1.54 ± 0.239
3.757ValIle: 3.757 ± 0.678
3.08ValLys: 3.08 ± 0.42
5.482ValLeu: 5.482 ± 0.604
1.109ValMet: 1.109 ± 0.31
2.71ValAsn: 2.71 ± 0.423
3.696ValPro: 3.696 ± 0.481
2.217ValGln: 2.217 ± 0.382
5.728ValArg: 5.728 ± 0.678
4.373ValSer: 4.373 ± 0.456
4.804ValThr: 4.804 ± 0.507
4.435ValVal: 4.435 ± 0.656
1.478ValTrp: 1.478 ± 0.308
2.279ValTyr: 2.279 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
1.478TrpAla: 1.478 ± 0.323
0.308TrpCys: 0.308 ± 0.119
1.54TrpAsp: 1.54 ± 0.316
0.801TrpGlu: 0.801 ± 0.205
0.801TrpPhe: 0.801 ± 0.214
1.786TrpGly: 1.786 ± 0.37
0.37TrpHis: 0.37 ± 0.188
1.232TrpIle: 1.232 ± 0.25
0.431TrpLys: 0.431 ± 0.229
1.725TrpLeu: 1.725 ± 0.299
0.246TrpMet: 0.246 ± 0.112
0.739TrpAsn: 0.739 ± 0.224
0.678TrpPro: 0.678 ± 0.237
0.739TrpGln: 0.739 ± 0.215
1.294TrpArg: 1.294 ± 0.276
1.109TrpSer: 1.109 ± 0.239
1.601TrpThr: 1.601 ± 0.38
2.033TrpVal: 2.033 ± 0.31
0.616TrpTrp: 0.616 ± 0.256
0.493TrpTyr: 0.493 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.587TyrAla: 2.587 ± 0.412
0.246TyrCys: 0.246 ± 0.164
1.232TyrAsp: 1.232 ± 0.31
2.279TyrGlu: 2.279 ± 0.342
0.431TyrPhe: 0.431 ± 0.124
2.464TyrGly: 2.464 ± 0.338
0.678TyrHis: 0.678 ± 0.226
1.663TyrIle: 1.663 ± 0.393
1.294TyrLys: 1.294 ± 0.219
2.71TyrLeu: 2.71 ± 0.415
0.554TyrMet: 0.554 ± 0.172
1.294TyrAsn: 1.294 ± 0.284
1.232TyrPro: 1.232 ± 0.269
1.109TyrGln: 1.109 ± 0.244
2.587TyrArg: 2.587 ± 0.371
1.725TyrSer: 1.725 ± 0.376
2.033TyrThr: 2.033 ± 0.372
1.909TyrVal: 1.909 ± 0.382
0.616TyrTrp: 0.616 ± 0.209
0.616TyrTyr: 0.616 ± 0.191
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (16236 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski