Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Pelotomaculum; Pelotomaculum thermopropionicum

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2884 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5D4P7|A5D4P7_PELTS Secreted protein OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=PTH_0613 PE=4 SV=1
MM1 pKa = 7.26VLLALIIGGLNIEE14 pKa = 4.34IQSGGIFNYY23 pKa = 10.85GEE25 pKa = 3.92IALNCAKK32 pKa = 10.85SNIHH36 pKa = 6.13QEE38 pKa = 3.32ADD40 pKa = 3.25ANSFCVGDD48 pKa = 3.72GNLFYY53 pKa = 10.32GTTDD57 pKa = 3.28QPSVSVNGIITDD69 pKa = 4.31IIDD72 pKa = 3.48GDD74 pKa = 4.06GARR77 pKa = 11.84TILPP81 pKa = 3.9

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5D0X0|A5D0X0_PELTS Fe2+ transport system protein A OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=FeoA PE=4 SV=1
MM1 pKa = 7.68PKK3 pKa = 9.97IKK5 pKa = 8.1THH7 pKa = 6.4RR8 pKa = 11.84GAAKK12 pKa = 10.19RR13 pKa = 11.84FKK15 pKa = 9.43QTAGGKK21 pKa = 8.39WKK23 pKa = 10.31GSHH26 pKa = 6.53AFHH29 pKa = 6.8SHH31 pKa = 5.62ILGKK35 pKa = 8.86KK36 pKa = 4.34TAKK39 pKa = 10.01RR40 pKa = 11.84KK41 pKa = 9.25RR42 pKa = 11.84NLRR45 pKa = 11.84KK46 pKa = 8.81VTVISMADD54 pKa = 3.07ARR56 pKa = 11.84RR57 pKa = 11.84LKK59 pKa = 10.63RR60 pKa = 11.84LLPP63 pKa = 4.21

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2884

0

2884

856099

30

2071

296.8

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.532 ± 0.06

1.269 ± 0.019

4.603 ± 0.028

7.23 ± 0.047

3.888 ± 0.034

8.51 ± 0.046

1.682 ± 0.022

6.165 ± 0.038

5.637 ± 0.04

10.294 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.023

3.318 ± 0.028

4.609 ± 0.036

3.004 ± 0.027

6.485 ± 0.043

5.02 ± 0.033

4.508 ± 0.033

7.802 ± 0.039

0.983 ± 0.016

3.074 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski