Porcine faeces associated circular DNA molecule-1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MGI1|A0A168MGI1_9VIRU ATP-dependent helicase Rep OS=Porcine faeces associated circular DNA molecule-1 OX=1843772 PE=3 SV=1
MM1 pKa = 7.47TPTPKK6 pKa = 10.09KK7 pKa = 10.39AYY9 pKa = 10.37CFTLNNYY16 pKa = 8.39TDD18 pKa = 4.1AEE20 pKa = 4.51VARR23 pKa = 11.84VHH25 pKa = 5.63GTCRR29 pKa = 11.84DD30 pKa = 3.42LSQFAIFGYY39 pKa = 10.2EE40 pKa = 3.91VGEE43 pKa = 4.04QGTPHH48 pKa = 5.89LQGYY52 pKa = 9.32IRR54 pKa = 11.84FAKK57 pKa = 9.86SYY59 pKa = 10.51RR60 pKa = 11.84FATIKK65 pKa = 10.75DD66 pKa = 3.45RR67 pKa = 11.84LLPRR71 pKa = 11.84CHH73 pKa = 7.54IEE75 pKa = 3.97VANGSARR82 pKa = 11.84QNRR85 pKa = 11.84EE86 pKa = 3.72YY87 pKa = 10.51CSKK90 pKa = 10.55SGRR93 pKa = 11.84FEE95 pKa = 4.39EE96 pKa = 5.57FGSCPDD102 pKa = 3.14EE103 pKa = 4.54PEE105 pKa = 3.81RR106 pKa = 11.84KK107 pKa = 7.74TRR109 pKa = 11.84DD110 pKa = 3.02EE111 pKa = 3.97LARR114 pKa = 11.84EE115 pKa = 4.56FVRR118 pKa = 11.84EE119 pKa = 4.02AGDD122 pKa = 3.11GRR124 pKa = 11.84RR125 pKa = 11.84GVRR128 pKa = 11.84RR129 pKa = 11.84FAEE132 pKa = 4.42SNPGTFYY139 pKa = 11.33YY140 pKa = 10.59SGAQLLRR147 pKa = 11.84NYY149 pKa = 10.84LSIQPAEE156 pKa = 4.04PRR158 pKa = 11.84PEE160 pKa = 3.69VRR162 pKa = 11.84VRR164 pKa = 11.84WFHH167 pKa = 6.1GLPGVGKK174 pKa = 9.53SRR176 pKa = 11.84RR177 pKa = 11.84AHH179 pKa = 6.09EE180 pKa = 4.7EE181 pKa = 3.61LPEE184 pKa = 5.1AYY186 pKa = 10.16VKK188 pKa = 10.54EE189 pKa = 4.48PRR191 pKa = 11.84TKK193 pKa = 8.57WWNNYY198 pKa = 8.99FGEE201 pKa = 4.43KK202 pKa = 10.25DD203 pKa = 3.71VIIDD207 pKa = 3.65DD208 pKa = 4.81FGPLGIDD215 pKa = 3.07INHH218 pKa = 7.43LLRR221 pKa = 11.84WFDD224 pKa = 3.4RR225 pKa = 11.84YY226 pKa = 11.13KK227 pKa = 11.08CFVEE231 pKa = 4.34YY232 pKa = 10.31KK233 pKa = 10.58GGMLPLVADD242 pKa = 3.85NFIVTSNFHH251 pKa = 6.77PYY253 pKa = 10.81DD254 pKa = 3.39VFKK257 pKa = 11.06DD258 pKa = 3.81KK259 pKa = 11.24EE260 pKa = 4.66GVPHH264 pKa = 6.79PQTEE268 pKa = 4.0ALLRR272 pKa = 11.84RR273 pKa = 11.84VVITLMEE280 pKa = 4.18

Molecular weight: 32.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MGI1|A0A168MGI1_9VIRU ATP-dependent helicase Rep OS=Porcine faeces associated circular DNA molecule-1 OX=1843772 PE=3 SV=1
MM1 pKa = 7.47TPTPKK6 pKa = 10.09KK7 pKa = 10.39AYY9 pKa = 10.37CFTLNNYY16 pKa = 8.39TDD18 pKa = 4.1AEE20 pKa = 4.51VARR23 pKa = 11.84VHH25 pKa = 5.63GTCRR29 pKa = 11.84DD30 pKa = 3.42LSQFAIFGYY39 pKa = 10.2EE40 pKa = 3.91VGEE43 pKa = 4.04QGTPHH48 pKa = 5.89LQGYY52 pKa = 9.32IRR54 pKa = 11.84FAKK57 pKa = 9.86SYY59 pKa = 10.51RR60 pKa = 11.84FATIKK65 pKa = 10.75DD66 pKa = 3.45RR67 pKa = 11.84LLPRR71 pKa = 11.84CHH73 pKa = 7.54IEE75 pKa = 3.97VANGSARR82 pKa = 11.84QNRR85 pKa = 11.84EE86 pKa = 3.72YY87 pKa = 10.51CSKK90 pKa = 10.55SGRR93 pKa = 11.84FEE95 pKa = 4.39EE96 pKa = 5.57FGSCPDD102 pKa = 3.14EE103 pKa = 4.54PEE105 pKa = 3.81RR106 pKa = 11.84KK107 pKa = 7.74TRR109 pKa = 11.84DD110 pKa = 3.02EE111 pKa = 3.97LARR114 pKa = 11.84EE115 pKa = 4.56FVRR118 pKa = 11.84EE119 pKa = 4.02AGDD122 pKa = 3.11GRR124 pKa = 11.84RR125 pKa = 11.84GVRR128 pKa = 11.84RR129 pKa = 11.84FAEE132 pKa = 4.42SNPGTFYY139 pKa = 11.33YY140 pKa = 10.59SGAQLLRR147 pKa = 11.84NYY149 pKa = 10.84LSIQPAEE156 pKa = 4.04PRR158 pKa = 11.84PEE160 pKa = 3.69VRR162 pKa = 11.84VRR164 pKa = 11.84WFHH167 pKa = 6.1GLPGVGKK174 pKa = 9.53SRR176 pKa = 11.84RR177 pKa = 11.84AHH179 pKa = 6.09EE180 pKa = 4.7EE181 pKa = 3.61LPEE184 pKa = 5.1AYY186 pKa = 10.16VKK188 pKa = 10.54EE189 pKa = 4.48PRR191 pKa = 11.84TKK193 pKa = 8.57WWNNYY198 pKa = 8.99FGEE201 pKa = 4.43KK202 pKa = 10.25DD203 pKa = 3.71VIIDD207 pKa = 3.65DD208 pKa = 4.81FGPLGIDD215 pKa = 3.07INHH218 pKa = 7.43LLRR221 pKa = 11.84WFDD224 pKa = 3.4RR225 pKa = 11.84YY226 pKa = 11.13KK227 pKa = 11.08CFVEE231 pKa = 4.34YY232 pKa = 10.31KK233 pKa = 10.58GGMLPLVADD242 pKa = 3.85NFIVTSNFHH251 pKa = 6.77PYY253 pKa = 10.81DD254 pKa = 3.39VFKK257 pKa = 11.06DD258 pKa = 3.81KK259 pKa = 11.24EE260 pKa = 4.66GVPHH264 pKa = 6.79PQTEE268 pKa = 4.0ALLRR272 pKa = 11.84RR273 pKa = 11.84VVITLMEE280 pKa = 4.18

Molecular weight: 32.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

280

280

280

280.0

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.071 ± 0.0

2.143 ± 0.0

5.0 ± 0.0

8.571 ± 0.0

6.429 ± 0.0

7.857 ± 0.0

2.857 ± 0.0

3.929 ± 0.0

5.0 ± 0.0

6.786 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.071 ± 0.0

3.929 ± 0.0

6.429 ± 0.0

2.5 ± 0.0

10.0 ± 0.0

3.929 ± 0.0

4.643 ± 0.0

6.429 ± 0.0

1.429 ± 0.0

5.0 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski