Sewage-associated gemycircularvirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus sewopo2; Sewage derived gemykibivirus 2

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL77|A0A0A7CL77_9VIRU Capsid protein OS=Sewage-associated gemycircularvirus 2 OX=1519407 PE=4 SV=1
MM1 pKa = 7.63PNAVTWKK8 pKa = 9.7LRR10 pKa = 11.84YY11 pKa = 10.17GIVTYY16 pKa = 9.59AQCSDD21 pKa = 3.65LDD23 pKa = 3.53PWKK26 pKa = 10.71VVDD29 pKa = 3.8MFATISAEE37 pKa = 4.06CIIGRR42 pKa = 11.84EE43 pKa = 3.95YY44 pKa = 11.1HH45 pKa = 6.53EE46 pKa = 5.79DD47 pKa = 3.46GGIHH51 pKa = 6.01LHH53 pKa = 6.45CFVDD57 pKa = 4.47FGRR60 pKa = 11.84IFRR63 pKa = 11.84SRR65 pKa = 11.84RR66 pKa = 11.84VDD68 pKa = 3.31VFDD71 pKa = 4.17VEE73 pKa = 4.89GRR75 pKa = 11.84HH76 pKa = 6.44PNVQHH81 pKa = 6.45VGRR84 pKa = 11.84TPWVAYY90 pKa = 9.42DD91 pKa = 3.46YY92 pKa = 10.52CIKK95 pKa = 10.8EE96 pKa = 4.07GDD98 pKa = 4.06VVAGAAEE105 pKa = 4.07RR106 pKa = 11.84PKK108 pKa = 10.43EE109 pKa = 3.94RR110 pKa = 11.84RR111 pKa = 11.84VGLSKK116 pKa = 10.44PDD118 pKa = 4.19EE119 pKa = 4.01IWTEE123 pKa = 3.62IMAATDD129 pKa = 3.53RR130 pKa = 11.84EE131 pKa = 4.63TFFYY135 pKa = 10.63LCQQLAPRR143 pKa = 11.84SLCLSFNSITAFAEE157 pKa = 3.47WKK159 pKa = 10.28YY160 pKa = 10.87RR161 pKa = 11.84VDD163 pKa = 3.58PEE165 pKa = 4.73PYY167 pKa = 8.73QHH169 pKa = 7.43PDD171 pKa = 3.32GIEE174 pKa = 3.77FLEE177 pKa = 4.32DD178 pKa = 2.99RR179 pKa = 11.84VGEE182 pKa = 3.87LHH184 pKa = 6.92RR185 pKa = 11.84WAEE188 pKa = 4.08ANLGLRR194 pKa = 11.84TGEE197 pKa = 4.05RR198 pKa = 11.84ARR200 pKa = 11.84SLVLIGPTRR209 pKa = 11.84VGKK212 pKa = 7.32TLWARR217 pKa = 11.84SLGNHH222 pKa = 7.12AYY224 pKa = 10.41FGGLFSLDD232 pKa = 3.43EE233 pKa = 4.11SLKK236 pKa = 9.9DD237 pKa = 3.17VEE239 pKa = 4.49YY240 pKa = 11.05AVFDD244 pKa = 4.09DD245 pKa = 4.2MMGGLEE251 pKa = 4.37FFHH254 pKa = 7.34SYY256 pKa = 10.65KK257 pKa = 10.45FWLGAQKK264 pKa = 10.36QFYY267 pKa = 8.95ATDD270 pKa = 3.38KK271 pKa = 10.64YY272 pKa = 10.71KK273 pKa = 10.99GKK275 pKa = 10.52RR276 pKa = 11.84LVDD279 pKa = 3.34WGRR282 pKa = 11.84PCIYY286 pKa = 10.01CHH288 pKa = 6.66NKK290 pKa = 10.08DD291 pKa = 3.57PRR293 pKa = 11.84LDD295 pKa = 3.67PKK297 pKa = 11.36ADD299 pKa = 3.67IDD301 pKa = 3.93WLEE304 pKa = 4.25GNCDD308 pKa = 4.82FITVEE313 pKa = 5.07DD314 pKa = 4.0SLLVV318 pKa = 3.6

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL77|A0A0A7CL77_9VIRU Capsid protein OS=Sewage-associated gemycircularvirus 2 OX=1519407 PE=4 SV=1
MM1 pKa = 7.66ALRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84LRR8 pKa = 11.84RR9 pKa = 11.84PTARR13 pKa = 11.84VRR15 pKa = 11.84PSRR18 pKa = 11.84ASGFRR23 pKa = 11.84RR24 pKa = 11.84VARR27 pKa = 11.84GRR29 pKa = 11.84RR30 pKa = 11.84SRR32 pKa = 11.84FTRR35 pKa = 11.84SRR37 pKa = 11.84RR38 pKa = 11.84IRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84PMTNRR47 pKa = 11.84RR48 pKa = 11.84ILNVSSKK55 pKa = 10.86KK56 pKa = 10.48KK57 pKa = 9.95VDD59 pKa = 3.06TMMPLVIGNDD69 pKa = 3.85GVGTEE74 pKa = 4.49GPLLVTPAEE83 pKa = 4.45PGQGFNSLYY92 pKa = 10.17IPSARR97 pKa = 11.84DD98 pKa = 3.45YY99 pKa = 10.91YY100 pKa = 10.04NTNSSHH106 pKa = 6.11ARR108 pKa = 11.84SAEE111 pKa = 4.1TIFVRR116 pKa = 11.84GYY118 pKa = 9.64KK119 pKa = 9.88EE120 pKa = 3.87RR121 pKa = 11.84VNVTVSGGATWTWRR135 pKa = 11.84RR136 pKa = 11.84TVFCMKK142 pKa = 10.71GDD144 pKa = 3.86MLRR147 pKa = 11.84DD148 pKa = 3.11AWTDD152 pKa = 3.14NSQPPQYY159 pKa = 11.01DD160 pKa = 3.28SLGPMAGQTPTRR172 pKa = 11.84SIGPMDD178 pKa = 5.01TILADD183 pKa = 4.0VIRR186 pKa = 11.84GLLYY190 pKa = 10.63RR191 pKa = 11.84GVQGSDD197 pKa = 2.2WYY199 pKa = 10.59DD200 pKa = 3.81PYY202 pKa = 10.65TAPIDD207 pKa = 3.84TTRR210 pKa = 11.84VTLLRR215 pKa = 11.84DD216 pKa = 3.26QVITINPTNDD226 pKa = 2.86SGRR229 pKa = 11.84TRR231 pKa = 11.84MYY233 pKa = 10.75RR234 pKa = 11.84FWQPINKK241 pKa = 8.77NLAYY245 pKa = 10.03QSYY248 pKa = 9.78EE249 pKa = 3.85NDD251 pKa = 3.07EE252 pKa = 4.54STVSGAYY259 pKa = 8.88SVRR262 pKa = 11.84SKK264 pKa = 10.97PGIGDD269 pKa = 3.61IYY271 pKa = 10.72IFDD274 pKa = 3.56QVRR277 pKa = 11.84RR278 pKa = 11.84EE279 pKa = 4.02TGAATASFRR288 pKa = 11.84FSPEE292 pKa = 3.2GTFYY296 pKa = 9.87WHH298 pKa = 6.63EE299 pKa = 4.02RR300 pKa = 3.52

Molecular weight: 34.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

820

202

318

273.3

31.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.707 ± 0.316

1.951 ± 0.703

6.951 ± 0.639

5.732 ± 1.189

4.756 ± 0.625

7.805 ± 0.119

2.317 ± 0.716

5.122 ± 0.256

3.537 ± 0.607

6.463 ± 0.825

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.073 ± 0.402

2.927 ± 0.61

5.244 ± 0.498

2.561 ± 0.205

10.122 ± 1.716

5.366 ± 1.143

6.098 ± 1.293

7.073 ± 0.34

2.683 ± 0.303

4.512 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski