Rhodanobacter sp. 115

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Rhodanobacter; unclassified Rhodanobacter

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4VSA3|I4VSA3_9GAMM Putative mercury resistance protein OS=Rhodanobacter sp. 115 OX=1162282 GN=UU5_15118 PE=4 SV=1
MM1 pKa = 7.51SCDD4 pKa = 4.17DD5 pKa = 4.11DD6 pKa = 4.74AGCVGEE12 pKa = 6.1DD13 pKa = 3.91FLPDD17 pKa = 3.65CFTFDD22 pKa = 3.55GVAGLGALDD31 pKa = 4.0EE32 pKa = 4.55AAGMLMPGMLLMSIGAVLLDD52 pKa = 4.38AGTLCALAASGSASHH67 pKa = 6.42SAASSAEE74 pKa = 3.93RR75 pKa = 11.84FKK77 pKa = 11.5

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4WMD2|I4WMD2_9GAMM Uncharacterized protein (Fragment) OS=Rhodanobacter sp. 115 OX=1162282 GN=UU5_02267 PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84QPGSGRR12 pKa = 11.84VRR14 pKa = 11.84GINLPRR20 pKa = 11.84LTIMIRR26 pKa = 11.84KK27 pKa = 8.76LAIATLLVAGVALSGSVFAQSAAAAPQAATHH58 pKa = 6.29SMTATKK64 pKa = 8.06TTAKK68 pKa = 9.47HH69 pKa = 4.66HH70 pKa = 6.14HH71 pKa = 6.4AKK73 pKa = 9.9KK74 pKa = 9.31HH75 pKa = 5.85HH76 pKa = 5.71KK77 pKa = 9.1HH78 pKa = 4.94HH79 pKa = 6.91HH80 pKa = 5.52KK81 pKa = 10.84AHH83 pKa = 6.31AKK85 pKa = 9.66KK86 pKa = 10.36AATTAAAGGKK96 pKa = 9.29

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3918

0

3918

1152151

16

2884

294.1

32.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.456 ± 0.052

0.911 ± 0.012

5.751 ± 0.028

4.839 ± 0.043

3.391 ± 0.024

8.407 ± 0.043

2.826 ± 0.021

4.201 ± 0.026

3.046 ± 0.034

10.904 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.019

2.599 ± 0.028

5.351 ± 0.025

3.8 ± 0.025

7.276 ± 0.05

5.366 ± 0.025

5.115 ± 0.028

7.339 ± 0.037

1.634 ± 0.02

2.449 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski