Lactococcus phage 53801

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BKP2|A0A1P8BKP2_9CAUD Antirepressor OS=Lactococcus phage 53801 OX=1868849 GN=DS53801_07 PE=4 SV=1
MM1 pKa = 7.52GMSCYY6 pKa = 9.51MICPNCGKK14 pKa = 8.6EE15 pKa = 4.12TIGRR19 pKa = 11.84YY20 pKa = 8.08ATDD23 pKa = 4.18DD24 pKa = 4.04SLCGEE29 pKa = 4.73CSDD32 pKa = 5.19NSSNTDD38 pKa = 3.08KK39 pKa = 11.44LSVEE43 pKa = 4.25KK44 pKa = 10.72LQEE47 pKa = 3.93QLNTAKK53 pKa = 10.71KK54 pKa = 9.47ALAEE58 pKa = 3.84IAEE61 pKa = 4.16ILNDD65 pKa = 5.07KK66 pKa = 10.25IHH68 pKa = 6.6SCDD71 pKa = 3.48NFCEE75 pKa = 4.29FDD77 pKa = 3.42GSLNMYY83 pKa = 10.06SVEE86 pKa = 4.23DD87 pKa = 3.79VQSVIDD93 pKa = 3.99SSLAAIGGDD102 pKa = 3.15RR103 pKa = 4.17

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BKN3|A0A1P8BKN3_9CAUD Uncharacterized protein OS=Lactococcus phage 53801 OX=1868849 GN=DS53801_03 PE=4 SV=1
MM1 pKa = 7.28GVNRR5 pKa = 11.84KK6 pKa = 8.96CPKK9 pKa = 10.0CGSTHH14 pKa = 5.31VQLSSQKK21 pKa = 11.15SKK23 pKa = 10.38MGWGCLGMIFFGIFYY38 pKa = 10.25FMHH41 pKa = 7.98IFMKK45 pKa = 9.92WAVGLMLFIFVDD57 pKa = 3.91WYY59 pKa = 9.61MAIIKK64 pKa = 9.54AAQNKK69 pKa = 8.78GYY71 pKa = 9.77VWRR74 pKa = 11.84SKK76 pKa = 10.35KK77 pKa = 9.32WFSGKK82 pKa = 9.72KK83 pKa = 6.66QTFFCHH89 pKa = 6.95DD90 pKa = 3.66CGNNFSAA97 pKa = 5.58

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11314

38

961

213.5

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.496 ± 0.414

0.734 ± 0.124

6.381 ± 0.191

6.797 ± 0.519

4.49 ± 0.24

6.762 ± 0.507

1.423 ± 0.166

6.947 ± 0.285

8.061 ± 0.423

7.654 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.166

6.355 ± 0.226

2.607 ± 0.184

4.472 ± 0.267

3.624 ± 0.21

7.0 ± 0.284

6.488 ± 0.405

5.94 ± 0.248

1.432 ± 0.117

3.792 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski