Silicibacter phage DSS3-P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8DCV7|G8DCV7_9CAUD DNA methyltransferase OS=Silicibacter phage DSS3-P1 OX=754060 GN=SDSG_00072 PE=3 SV=1
MM1 pKa = 7.46SNTYY5 pKa = 8.72TVAVVEE11 pKa = 4.9GSTPLPPCYY20 pKa = 9.19PDD22 pKa = 3.63PLLYY26 pKa = 10.62VVTVSDD32 pKa = 3.7PHH34 pKa = 8.21DD35 pKa = 4.06SEE37 pKa = 6.28SILDD41 pKa = 3.7QVAEE45 pKa = 4.07ARR47 pKa = 11.84ALEE50 pKa = 4.07LLGEE54 pKa = 4.49PEE56 pKa = 5.59DD57 pKa = 4.07YY58 pKa = 10.73TPQTFDD64 pKa = 3.39ALRR67 pKa = 11.84AGLRR71 pKa = 11.84LLFVLAGDD79 pKa = 4.45VYY81 pKa = 10.73PVADD85 pKa = 4.78FRR87 pKa = 11.84QSS89 pKa = 2.73

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8DCQ9|G8DCQ9_9CAUD Uncharacterized protein OS=Silicibacter phage DSS3-P1 OX=754060 GN=SDSG_00023 PE=4 SV=1
MM1 pKa = 7.53NLPDD5 pKa = 4.36TNPKK9 pKa = 8.7TRR11 pKa = 11.84FGMAKK16 pKa = 9.21PGIYY20 pKa = 10.11AIPPTALLHH29 pKa = 6.65CGAAMHH35 pKa = 6.51NGCEE39 pKa = 4.67KK40 pKa = 11.02YY41 pKa = 10.87GLTNWRR47 pKa = 11.84EE48 pKa = 4.25HH49 pKa = 5.82EE50 pKa = 4.43VSASVYY56 pKa = 8.31YY57 pKa = 10.57NAAFRR62 pKa = 11.84HH63 pKa = 5.37LAAWWDD69 pKa = 3.84GEE71 pKa = 4.59RR72 pKa = 11.84EE73 pKa = 4.22AEE75 pKa = 4.25DD76 pKa = 3.6SGVHH80 pKa = 5.84HH81 pKa = 7.46LGHH84 pKa = 6.81VMACCAVLLDD94 pKa = 4.27AEE96 pKa = 4.68HH97 pKa = 6.37MGKK100 pKa = 9.71LRR102 pKa = 11.84DD103 pKa = 4.35DD104 pKa = 3.86RR105 pKa = 11.84PAIPGPFSFAVAEE118 pKa = 4.2MTKK121 pKa = 10.08PQAEE125 pKa = 4.32EE126 pKa = 5.15GKK128 pKa = 7.95QTWGGFCQDD137 pKa = 2.83IAASAEE143 pKa = 3.85AAFAKK148 pKa = 10.59HH149 pKa = 5.81EE150 pKa = 4.34ADD152 pKa = 3.57EE153 pKa = 4.12QLEE156 pKa = 4.13ADD158 pKa = 4.33CEE160 pKa = 4.29EE161 pKa = 3.86IALRR165 pKa = 11.84VLSTGSLDD173 pKa = 3.5DD174 pKa = 3.87AKK176 pKa = 11.19AVIRR180 pKa = 11.84AYY182 pKa = 9.74LTGGGTTRR190 pKa = 11.84KK191 pKa = 9.64ISGRR195 pKa = 11.84LLATPSIGKK204 pKa = 8.45MSRR207 pKa = 11.84PRR209 pKa = 11.84STLSRR214 pKa = 11.84RR215 pKa = 11.84PRR217 pKa = 11.84TPVSPLGSTQARR229 pKa = 11.84APEE232 pKa = 3.89PAMTGMLSSRR242 pKa = 11.84SSRR245 pKa = 11.84RR246 pKa = 11.84LPTLNWSRR254 pKa = 11.84ASGRR258 pKa = 11.84SWIKK262 pKa = 9.7TGPDD266 pKa = 3.16GSRR269 pKa = 11.84SLRR272 pKa = 11.84FSATTSVPSGSRR284 pKa = 11.84PTVRR288 pKa = 11.84TTGPGSRR295 pKa = 11.84STSDD299 pKa = 2.82ARR301 pKa = 11.84PRR303 pKa = 11.84DD304 pKa = 3.79STHH307 pKa = 7.1RR308 pKa = 11.84PPSWTMPSAWPVSSSEE324 pKa = 3.98PPDD327 pKa = 2.98RR328 pKa = 11.84CRR330 pKa = 11.84PLTPDD335 pKa = 2.83SPRR338 pKa = 11.84PPRR341 pKa = 11.84IAGCVAPAPSACRR354 pKa = 11.84TRR356 pKa = 11.84SRR358 pKa = 11.84KK359 pKa = 9.13RR360 pKa = 11.84APASSPPRR368 pKa = 11.84GPWRR372 pKa = 11.84TTSGRR377 pKa = 11.84CAWFSAWSRR386 pKa = 11.84RR387 pKa = 11.84ISWDD391 pKa = 3.09RR392 pKa = 11.84KK393 pKa = 7.67SEE395 pKa = 4.14RR396 pKa = 11.84TVSPSPSRR404 pKa = 11.84TRR406 pKa = 11.84WWRR409 pKa = 11.84RR410 pKa = 2.92

Molecular weight: 44.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

17487

54

1744

224.2

24.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.62 ± 0.628

0.858 ± 0.11

6.496 ± 0.255

7.005 ± 0.272

3.362 ± 0.162

8.12 ± 0.276

2.047 ± 0.196

4.34 ± 0.177

3.923 ± 0.318

8.595 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.162

3.162 ± 0.165

5.227 ± 0.431

3.38 ± 0.288

7.566 ± 0.32

5.267 ± 0.228

5.999 ± 0.283

6.473 ± 0.194

1.698 ± 0.185

2.533 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski