Klebsormidium nitens (Green alga) (Ulothrix nitens)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1I0G7|A0A1Y1I0G7_KLENI Rhodanese domain-containing protein OS=Klebsormidium nitens OX=105231 GN=KFL_001490030 PE=4 SV=1
MM1 pKa = 7.76AGPSPLLEE9 pKa = 5.06DD10 pKa = 4.47LDD12 pKa = 3.92VDD14 pKa = 5.07IIADD18 pKa = 3.82EE19 pKa = 4.73CQDD22 pKa = 3.71SQCIPDD28 pKa = 3.65GMEE31 pKa = 3.91EE32 pKa = 4.46VVDD35 pKa = 4.27CCMSSSGAEE44 pKa = 3.52ADD46 pKa = 3.62FDD48 pKa = 4.99RR49 pKa = 11.84MVGALEE55 pKa = 5.9DD56 pKa = 4.61IITDD60 pKa = 3.43ADD62 pKa = 3.62FEE64 pKa = 4.28EE65 pKa = 5.07AQTSFCAAHH74 pKa = 6.21CAVFEE79 pKa = 5.14DD80 pKa = 3.83SDD82 pKa = 4.18EE83 pKa = 4.4NKK85 pKa = 10.5LEE87 pKa = 3.83YY88 pKa = 9.84MEE90 pKa = 6.04LFQQYY95 pKa = 10.26VDD97 pKa = 3.77VLDD100 pKa = 4.07GVIAARR106 pKa = 11.84LAEE109 pKa = 4.61ALPDD113 pKa = 3.93LPLHH117 pKa = 7.03DD118 pKa = 5.24FLHH121 pKa = 5.94QLSEE125 pKa = 4.13HH126 pKa = 6.63GEE128 pKa = 4.1AEE130 pKa = 3.88GDD132 pKa = 3.7VFDD135 pKa = 6.84FLMTLCDD142 pKa = 4.11FQAFKK147 pKa = 11.49DD148 pKa = 3.73LMLSYY153 pKa = 10.1KK154 pKa = 9.79QQAAGAVPSLQLLVQPMPLLTDD176 pKa = 3.74EE177 pKa = 5.23DD178 pKa = 4.54ASGDD182 pKa = 3.77ARR184 pKa = 11.84PDD186 pKa = 3.63LDD188 pKa = 3.37THH190 pKa = 6.33LQISPIRR197 pKa = 11.84RR198 pKa = 11.84QLAALTT204 pKa = 3.97

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1IM25|A0A1Y1IM25_KLENI Isoform of A0A1Y1IMB5 Cdc2-related protein kinase (Fragment) OS=Klebsormidium nitens OX=105231 GN=KFL_008880010 PE=4 SV=1
TT1 pKa = 7.26RR2 pKa = 11.84SSGRR6 pKa = 11.84RR7 pKa = 11.84SGARR11 pKa = 11.84KK12 pKa = 8.52AAARR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84PRR20 pKa = 11.84RR21 pKa = 11.84AGWRR25 pKa = 11.84RR26 pKa = 3.04

Molecular weight: 3.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16171

80

16251

8760576

9

7617

539.1

58.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.629 ± 0.029

1.451 ± 0.01

5.121 ± 0.012

7.207 ± 0.027

3.286 ± 0.013

8.45 ± 0.022

2.03 ± 0.008

3.311 ± 0.013

4.773 ± 0.018

9.327 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.84 ± 0.008

2.825 ± 0.011

6.118 ± 0.018

4.03 ± 0.013

6.919 ± 0.017

7.425 ± 0.018

5.083 ± 0.016

6.775 ± 0.013

1.305 ± 0.006

2.094 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski