Sphaeroforma arctica JP610

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Ichthyosporea; Ichthyophonida; Sphaeroforma; Sphaeroforma arctica

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0G0V7|A0A0L0G0V7_9EUKA Uncharacterized protein OS=Sphaeroforma arctica JP610 OX=667725 GN=SARC_05018 PE=4 SV=1
MM1 pKa = 7.75WKK3 pKa = 10.57GDD5 pKa = 3.99LNSEE9 pKa = 4.38EE10 pKa = 4.45LSHH13 pKa = 7.18AISACPSDD21 pKa = 4.93DD22 pKa = 3.16VRR24 pKa = 11.84LYY26 pKa = 10.11TGHH29 pKa = 7.8DD30 pKa = 4.81DD31 pKa = 3.54ITPLPSTVCLISEE44 pKa = 4.65PGSLTTSNAALYY56 pKa = 10.6ASTTIDD62 pKa = 3.96HH63 pKa = 7.7DD64 pKa = 5.07DD65 pKa = 3.73IPIDD69 pKa = 4.09SYY71 pKa = 11.97AKK73 pKa = 10.15SPPSSGSPDD82 pKa = 3.54PLATLHH88 pKa = 5.92STPSLEE94 pKa = 4.48GSSIMLTDD102 pKa = 4.97DD103 pKa = 3.44SDD105 pKa = 5.3ADD107 pKa = 3.92FSPSEE112 pKa = 4.03FVGNDD117 pKa = 3.27DD118 pKa = 4.14

Molecular weight: 12.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0FJS8|A0A0L0FJS8_9EUKA PKS_ER domain-containing protein OS=Sphaeroforma arctica JP610 OX=667725 GN=SARC_10495 PE=4 SV=1
GG1 pKa = 7.32PPQHH5 pKa = 7.1RR6 pKa = 11.84NGPPGQSFSPGPTSGPSGPPQGPPGQHH33 pKa = 6.22RR34 pKa = 11.84GSTGSPATPFAQNGPRR50 pKa = 11.84GPPGPSGPPGQQGQGPGHH68 pKa = 7.15PSMGPMSAPNAPGQGTPVRR87 pKa = 11.84GPNGQVLGPMGARR100 pKa = 11.84PNGPPGAVGLLLVSVV115 pKa = 4.5

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18207

442

18649

5628458

29

5930

301.8

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.206 ± 0.02

1.8 ± 0.022

6.143 ± 0.018

6.053 ± 0.024

3.053 ± 0.017

6.717 ± 0.022

2.774 ± 0.014

4.487 ± 0.017

5.179 ± 0.022

7.987 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.011

4.341 ± 0.013

4.842 ± 0.021

4.249 ± 0.027

5.608 ± 0.023

8.208 ± 0.023

7.043 ± 0.025

6.819 ± 0.02

1.008 ± 0.007

2.843 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski