Amino acid dipepetide frequency for Pseudomonas sp. K2W31S-8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.946AlaAla: 14.946 ± 0.149
1.306AlaCys: 1.306 ± 0.035
6.183AlaAsp: 6.183 ± 0.08
8.149AlaGlu: 8.149 ± 0.095
3.849AlaPhe: 3.849 ± 0.062
10.069AlaGly: 10.069 ± 0.101
2.287AlaHis: 2.287 ± 0.036
5.172AlaIle: 5.172 ± 0.066
4.019AlaLys: 4.019 ± 0.07
14.636AlaLeu: 14.636 ± 0.145
2.839AlaMet: 2.839 ± 0.044
3.0AlaAsn: 3.0 ± 0.061
5.147AlaPro: 5.147 ± 0.065
5.538AlaGln: 5.538 ± 0.067
7.565AlaArg: 7.565 ± 0.092
6.247AlaSer: 6.247 ± 0.071
4.777AlaThr: 4.777 ± 0.077
7.748AlaVal: 7.748 ± 0.081
1.718AlaTrp: 1.718 ± 0.043
2.631AlaTyr: 2.631 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
1.256CysAla: 1.256 ± 0.035
0.146CysCys: 0.146 ± 0.012
0.51CysAsp: 0.51 ± 0.022
0.529CysGlu: 0.529 ± 0.021
0.342CysPhe: 0.342 ± 0.016
1.021CysGly: 1.021 ± 0.027
0.285CysHis: 0.285 ± 0.017
0.413CysIle: 0.413 ± 0.017
0.263CysLys: 0.263 ± 0.013
1.181CysLeu: 1.181 ± 0.031
0.19CysMet: 0.19 ± 0.013
0.254CysAsn: 0.254 ± 0.013
0.549CysPro: 0.549 ± 0.021
0.434CysGln: 0.434 ± 0.016
0.679CysArg: 0.679 ± 0.024
0.575CysSer: 0.575 ± 0.02
0.463CysThr: 0.463 ± 0.021
0.689CysVal: 0.689 ± 0.022
0.139CysTrp: 0.139 ± 0.01
0.25CysTyr: 0.25 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
5.744AspAla: 5.744 ± 0.087
0.567AspCys: 0.567 ± 0.021
2.792AspAsp: 2.792 ± 0.057
3.479AspGlu: 3.479 ± 0.057
2.087AspPhe: 2.087 ± 0.041
4.721AspGly: 4.721 ± 0.087
1.055AspHis: 1.055 ± 0.028
2.457AspIle: 2.457 ± 0.044
1.895AspLys: 1.895 ± 0.043
5.804AspLeu: 5.804 ± 0.072
1.095AspMet: 1.095 ± 0.033
1.595AspAsn: 1.595 ± 0.057
2.767AspPro: 2.767 ± 0.048
2.057AspGln: 2.057 ± 0.034
2.871AspArg: 2.871 ± 0.045
2.995AspSer: 2.995 ± 0.057
2.2AspThr: 2.2 ± 0.06
3.28AspVal: 3.28 ± 0.063
1.019AspTrp: 1.019 ± 0.032
1.813AspTyr: 1.813 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.945GluAla: 6.945 ± 0.081
0.445GluCys: 0.445 ± 0.022
2.407GluAsp: 2.407 ± 0.045
3.254GluGlu: 3.254 ± 0.059
1.903GluPhe: 1.903 ± 0.04
3.974GluGly: 3.974 ± 0.055
1.607GluHis: 1.607 ± 0.034
2.692GluIle: 2.692 ± 0.048
1.838GluLys: 1.838 ± 0.043
7.949GluLeu: 7.949 ± 0.081
1.245GluMet: 1.245 ± 0.03
1.357GluAsn: 1.357 ± 0.035
2.479GluPro: 2.479 ± 0.046
3.925GluGln: 3.925 ± 0.07
5.164GluArg: 5.164 ± 0.076
2.556GluSer: 2.556 ± 0.043
2.353GluThr: 2.353 ± 0.046
4.404GluVal: 4.404 ± 0.065
0.706GluTrp: 0.706 ± 0.02
1.174GluTyr: 1.174 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.379PheAla: 4.379 ± 0.061
0.464PheCys: 0.464 ± 0.016
2.387PheAsp: 2.387 ± 0.04
1.963PheGlu: 1.963 ± 0.041
1.401PhePhe: 1.401 ± 0.035
3.365PheGly: 3.365 ± 0.058
0.764PheHis: 0.764 ± 0.024
1.79PheIle: 1.79 ± 0.039
1.207PheLys: 1.207 ± 0.033
3.266PheLeu: 3.266 ± 0.06
0.778PheMet: 0.778 ± 0.023
1.239PheAsn: 1.239 ± 0.03
1.442PhePro: 1.442 ± 0.032
1.297PheGln: 1.297 ± 0.031
1.91PheArg: 1.91 ± 0.038
2.389PheSer: 2.389 ± 0.045
1.799PheThr: 1.799 ± 0.038
2.423PheVal: 2.423 ± 0.05
0.531PheTrp: 0.531 ± 0.02
1.062PheTyr: 1.062 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
8.113GlyAla: 8.113 ± 0.092
0.992GlyCys: 0.992 ± 0.028
4.128GlyAsp: 4.128 ± 0.078
5.188GlyGlu: 5.188 ± 0.064
3.323GlyPhe: 3.323 ± 0.055
6.667GlyGly: 6.667 ± 0.12
1.896GlyHis: 1.896 ± 0.036
4.06GlyIle: 4.06 ± 0.062
3.328GlyLys: 3.328 ± 0.06
9.797GlyLeu: 9.797 ± 0.1
2.14GlyMet: 2.14 ± 0.046
2.474GlyAsn: 2.474 ± 0.109
2.74GlyPro: 2.74 ± 0.045
4.033GlyGln: 4.033 ± 0.073
5.266GlyArg: 5.266 ± 0.069
4.787GlySer: 4.787 ± 0.069
3.749GlyThr: 3.749 ± 0.085
6.069GlyVal: 6.069 ± 0.066
1.338GlyTrp: 1.338 ± 0.032
2.446GlyTyr: 2.446 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.384HisAla: 2.384 ± 0.044
0.365HisCys: 0.365 ± 0.016
1.147HisAsp: 1.147 ± 0.029
1.225HisGlu: 1.225 ± 0.031
0.986HisPhe: 0.986 ± 0.026
2.073HisGly: 2.073 ± 0.042
0.592HisHis: 0.592 ± 0.019
0.973HisIle: 0.973 ± 0.031
0.639HisLys: 0.639 ± 0.021
2.668HisLeu: 2.668 ± 0.046
0.479HisMet: 0.479 ± 0.018
0.665HisAsn: 0.665 ± 0.023
1.449HisPro: 1.449 ± 0.032
0.898HisGln: 0.898 ± 0.029
1.307HisArg: 1.307 ± 0.032
1.246HisSer: 1.246 ± 0.031
0.941HisThr: 0.941 ± 0.025
1.273HisVal: 1.273 ± 0.034
0.44HisTrp: 0.44 ± 0.02
0.735HisTyr: 0.735 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.783IleAla: 5.783 ± 0.066
0.481IleCys: 0.481 ± 0.018
3.054IleAsp: 3.054 ± 0.051
3.101IleGlu: 3.101 ± 0.048
1.316IlePhe: 1.316 ± 0.034
4.503IleGly: 4.503 ± 0.061
0.934IleHis: 0.934 ± 0.026
1.768IleIle: 1.768 ± 0.042
1.523IleLys: 1.523 ± 0.041
4.02IleLeu: 4.02 ± 0.053
0.728IleMet: 0.728 ± 0.024
1.566IleAsn: 1.566 ± 0.034
2.198IlePro: 2.198 ± 0.044
1.594IleGln: 1.594 ± 0.034
2.833IleArg: 2.833 ± 0.049
2.708IleSer: 2.708 ± 0.051
2.239IleThr: 2.239 ± 0.044
2.965IleVal: 2.965 ± 0.047
0.446IleTrp: 0.446 ± 0.019
1.066IleTyr: 1.066 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.927LysAla: 3.927 ± 0.064
0.197LysCys: 0.197 ± 0.012
1.576LysAsp: 1.576 ± 0.038
1.547LysGlu: 1.547 ± 0.037
0.858LysPhe: 0.858 ± 0.03
2.42LysGly: 2.42 ± 0.043
0.733LysHis: 0.733 ± 0.023
1.518LysIle: 1.518 ± 0.037
1.169LysLys: 1.169 ± 0.039
3.796LysLeu: 3.796 ± 0.05
0.641LysMet: 0.641 ± 0.023
0.903LysAsn: 0.903 ± 0.029
2.029LysPro: 2.029 ± 0.057
1.624LysGln: 1.624 ± 0.034
2.382LysArg: 2.382 ± 0.043
1.736LysSer: 1.736 ± 0.039
1.691LysThr: 1.691 ± 0.043
2.595LysVal: 2.595 ± 0.047
0.319LysTrp: 0.319 ± 0.016
0.704LysTyr: 0.704 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
16.112LeuAla: 16.112 ± 0.14
1.311LeuCys: 1.311 ± 0.033
6.984LeuAsp: 6.984 ± 0.065
6.698LeuGlu: 6.698 ± 0.089
4.276LeuPhe: 4.276 ± 0.066
9.935LeuGly: 9.935 ± 0.098
2.594LeuHis: 2.594 ± 0.043
5.407LeuIle: 5.407 ± 0.069
4.3LeuLys: 4.3 ± 0.062
15.05LeuLeu: 15.05 ± 0.177
2.327LeuMet: 2.327 ± 0.042
3.558LeuAsn: 3.558 ± 0.053
6.583LeuPro: 6.583 ± 0.07
4.968LeuGln: 4.968 ± 0.079
7.904LeuArg: 7.904 ± 0.085
7.147LeuSer: 7.147 ± 0.078
5.467LeuThr: 5.467 ± 0.068
8.027LeuVal: 8.027 ± 0.079
1.508LeuTrp: 1.508 ± 0.036
2.67LeuTyr: 2.67 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.484MetAla: 2.484 ± 0.045
0.162MetCys: 0.162 ± 0.01
0.994MetAsp: 0.994 ± 0.028
0.878MetGlu: 0.878 ± 0.027
0.621MetPhe: 0.621 ± 0.022
1.559MetGly: 1.559 ± 0.039
0.503MetHis: 0.503 ± 0.02
0.966MetIle: 0.966 ± 0.022
0.767MetLys: 0.767 ± 0.024
2.546MetLeu: 2.546 ± 0.041
0.432MetMet: 0.432 ± 0.017
0.774MetAsn: 0.774 ± 0.023
1.245MetPro: 1.245 ± 0.028
1.003MetGln: 1.003 ± 0.023
1.483MetArg: 1.483 ± 0.032
1.624MetSer: 1.624 ± 0.03
1.253MetThr: 1.253 ± 0.031
1.379MetVal: 1.379 ± 0.035
0.175MetTrp: 0.175 ± 0.01
0.371MetTyr: 0.371 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.038AsnAla: 3.038 ± 0.056
0.293AsnCys: 0.293 ± 0.015
1.5AsnAsp: 1.5 ± 0.065
1.292AsnGlu: 1.292 ± 0.034
1.093AsnPhe: 1.093 ± 0.048
2.539AsnGly: 2.539 ± 0.07
0.604AsnHis: 0.604 ± 0.025
1.29AsnIle: 1.29 ± 0.035
0.846AsnLys: 0.846 ± 0.027
3.46AsnLeu: 3.46 ± 0.054
0.54AsnMet: 0.54 ± 0.019
0.907AsnAsn: 0.907 ± 0.031
2.018AsnPro: 2.018 ± 0.043
1.24AsnGln: 1.24 ± 0.033
1.788AsnArg: 1.788 ± 0.035
1.523AsnSer: 1.523 ± 0.037
1.285AsnThr: 1.285 ± 0.035
1.801AsnVal: 1.801 ± 0.038
0.432AsnTrp: 0.432 ± 0.019
0.812AsnTyr: 0.812 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
6.292ProAla: 6.292 ± 0.091
0.363ProCys: 0.363 ± 0.019
2.592ProAsp: 2.592 ± 0.043
3.221ProGlu: 3.221 ± 0.054
1.781ProPhe: 1.781 ± 0.033
4.122ProGly: 4.122 ± 0.056
1.088ProHis: 1.088 ± 0.031
1.859ProIle: 1.859 ± 0.036
1.501ProLys: 1.501 ± 0.036
6.214ProLeu: 6.214 ± 0.068
1.035ProMet: 1.035 ± 0.028
1.319ProAsn: 1.319 ± 0.028
2.08ProPro: 2.08 ± 0.045
2.542ProGln: 2.542 ± 0.045
2.78ProArg: 2.78 ± 0.054
2.664ProSer: 2.664 ± 0.049
2.137ProThr: 2.137 ± 0.037
3.636ProVal: 3.636 ± 0.06
0.736ProTrp: 0.736 ± 0.024
1.195ProTyr: 1.195 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
6.441GlnAla: 6.441 ± 0.084
0.309GlnCys: 0.309 ± 0.016
1.885GlnAsp: 1.885 ± 0.038
1.982GlnGlu: 1.982 ± 0.039
1.454GlnPhe: 1.454 ± 0.031
3.596GlnGly: 3.596 ± 0.046
1.19GlnHis: 1.19 ± 0.033
2.003GlnIle: 2.003 ± 0.039
1.129GlnLys: 1.129 ± 0.026
6.189GlnLeu: 6.189 ± 0.087
0.946GlnMet: 0.946 ± 0.028
1.015GlnAsn: 1.015 ± 0.025
2.726GlnPro: 2.726 ± 0.046
2.921GlnGln: 2.921 ± 0.066
3.922GlnArg: 3.922 ± 0.066
2.178GlnSer: 2.178 ± 0.044
1.826GlnThr: 1.826 ± 0.036
3.605GlnVal: 3.605 ± 0.061
0.662GlnTrp: 0.662 ± 0.024
1.013GlnTyr: 1.013 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
6.734ArgAla: 6.734 ± 0.087
0.631ArgCys: 0.631 ± 0.019
3.543ArgAsp: 3.543 ± 0.053
4.419ArgGlu: 4.419 ± 0.058
2.784ArgPhe: 2.784 ± 0.047
4.343ArgGly: 4.343 ± 0.063
1.755ArgHis: 1.755 ± 0.038
3.152ArgIle: 3.152 ± 0.053
1.918ArgLys: 1.918 ± 0.036
9.187ArgLeu: 9.187 ± 0.095
1.541ArgMet: 1.541 ± 0.032
1.774ArgAsn: 1.774 ± 0.032
2.837ArgPro: 2.837 ± 0.05
3.58ArgGln: 3.58 ± 0.063
4.605ArgArg: 4.605 ± 0.067
3.366ArgSer: 3.366 ± 0.057
2.539ArgThr: 2.539 ± 0.05
4.587ArgVal: 4.587 ± 0.069
1.1ArgTrp: 1.1 ± 0.028
2.11ArgTyr: 2.11 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.385SerAla: 6.385 ± 0.075
0.509SerCys: 0.509 ± 0.02
2.629SerAsp: 2.629 ± 0.056
3.047SerGlu: 3.047 ± 0.056
2.136SerPhe: 2.136 ± 0.043
5.344SerGly: 5.344 ± 0.087
1.29SerHis: 1.29 ± 0.031
2.495SerIle: 2.495 ± 0.045
1.683SerLys: 1.683 ± 0.041
7.204SerLeu: 7.204 ± 0.087
1.237SerMet: 1.237 ± 0.028
1.555SerAsn: 1.555 ± 0.033
2.603SerPro: 2.603 ± 0.048
2.349SerGln: 2.349 ± 0.047
3.533SerArg: 3.533 ± 0.059
3.2SerSer: 3.2 ± 0.051
2.554SerThr: 2.554 ± 0.045
3.6SerVal: 3.6 ± 0.06
0.798SerTrp: 0.798 ± 0.021
1.451SerTyr: 1.451 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.023ThrAla: 5.023 ± 0.075
0.445ThrCys: 0.445 ± 0.021
2.156ThrAsp: 2.156 ± 0.054
2.211ThrGlu: 2.211 ± 0.042
1.626ThrPhe: 1.626 ± 0.033
4.001ThrGly: 4.001 ± 0.08
0.968ThrHis: 0.968 ± 0.029
1.778ThrIle: 1.778 ± 0.05
1.023ThrLys: 1.023 ± 0.029
6.208ThrLeu: 6.208 ± 0.078
0.718ThrMet: 0.718 ± 0.021
1.074ThrAsn: 1.074 ± 0.038
2.982ThrPro: 2.982 ± 0.048
1.776ThrGln: 1.776 ± 0.038
2.847ThrArg: 2.847 ± 0.046
2.406ThrSer: 2.406 ± 0.045
2.153ThrThr: 2.153 ± 0.054
3.198ThrVal: 3.198 ± 0.06
0.664ThrTrp: 0.664 ± 0.024
1.148ThrTyr: 1.148 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
7.906ValAla: 7.906 ± 0.089
0.739ValCys: 0.739 ± 0.025
3.842ValAsp: 3.842 ± 0.059
4.503ValGlu: 4.503 ± 0.057
2.478ValPhe: 2.478 ± 0.046
5.206ValGly: 5.206 ± 0.074
1.372ValHis: 1.372 ± 0.033
3.496ValIle: 3.496 ± 0.057
2.234ValLys: 2.234 ± 0.042
8.185ValLeu: 8.185 ± 0.084
1.564ValMet: 1.564 ± 0.033
2.113ValAsn: 2.113 ± 0.043
3.255ValPro: 3.255 ± 0.049
2.788ValGln: 2.788 ± 0.045
4.443ValArg: 4.443 ± 0.054
3.941ValSer: 3.941 ± 0.051
3.311ValThr: 3.311 ± 0.055
5.059ValVal: 5.059 ± 0.07
0.813ValTrp: 0.813 ± 0.024
1.549ValTyr: 1.549 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.261TrpAla: 1.261 ± 0.03
0.155TrpCys: 0.155 ± 0.011
0.593TrpAsp: 0.593 ± 0.024
0.567TrpGlu: 0.567 ± 0.019
0.532TrpPhe: 0.532 ± 0.019
0.91TrpGly: 0.91 ± 0.027
0.398TrpHis: 0.398 ± 0.016
0.598TrpIle: 0.598 ± 0.02
0.399TrpLys: 0.399 ± 0.015
2.343TrpLeu: 2.343 ± 0.048
0.316TrpMet: 0.316 ± 0.013
0.453TrpAsn: 0.453 ± 0.02
0.675TrpPro: 0.675 ± 0.023
1.011TrpGln: 1.011 ± 0.032
1.154TrpArg: 1.154 ± 0.032
0.795TrpSer: 0.795 ± 0.024
0.574TrpThr: 0.574 ± 0.02
0.88TrpVal: 0.88 ± 0.026
0.236TrpTrp: 0.236 ± 0.011
0.343TrpTyr: 0.343 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.672TyrAla: 2.672 ± 0.047
0.289TyrCys: 0.289 ± 0.014
1.39TyrAsp: 1.39 ± 0.038
1.124TyrGlu: 1.124 ± 0.027
1.011TyrPhe: 1.011 ± 0.031
2.163TyrGly: 2.163 ± 0.046
0.57TyrHis: 0.57 ± 0.021
0.938TyrIle: 0.938 ± 0.025
0.683TyrLys: 0.683 ± 0.022
3.121TyrLeu: 3.121 ± 0.052
0.428TyrMet: 0.428 ± 0.017
0.708TyrAsn: 0.708 ± 0.024
1.353TyrPro: 1.353 ± 0.032
1.414TyrGln: 1.414 ± 0.036
2.068TyrArg: 2.068 ± 0.04
1.52TyrSer: 1.52 ± 0.036
1.095TyrThr: 1.095 ± 0.036
1.58TyrVal: 1.58 ± 0.032
0.413TyrTrp: 0.413 ± 0.016
0.739TyrTyr: 0.739 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4303 proteins (1412926 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski