Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago; Ustilago maydis

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4P999|Q4P999_USTMA Chromosome 8 whole genome shotgun sequence OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=eff1-4 PE=4 SV=1
MM1 pKa = 7.52PVSFYY6 pKa = 11.44DD7 pKa = 4.02EE8 pKa = 4.71IEE10 pKa = 4.52LEE12 pKa = 4.16DD13 pKa = 3.82MSYY16 pKa = 11.68DD17 pKa = 3.59DD18 pKa = 4.66EE19 pKa = 6.7KK20 pKa = 11.54DD21 pKa = 3.58VFHH24 pKa = 6.56YY25 pKa = 8.54PCPCGDD31 pKa = 3.15RR32 pKa = 11.84FEE34 pKa = 4.45ITRR37 pKa = 11.84QQLKK41 pKa = 10.26DD42 pKa = 3.44AEE44 pKa = 4.35DD45 pKa = 3.55VARR48 pKa = 11.84CPSCSLIIRR57 pKa = 11.84VVFDD61 pKa = 5.13PIDD64 pKa = 4.03FEE66 pKa = 7.64DD67 pKa = 4.62GDD69 pKa = 3.99EE70 pKa = 5.32DD71 pKa = 3.82EE72 pKa = 6.01APVNTIDD79 pKa = 3.95VASQAVAAAAA89 pKa = 3.72

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1DUI7|A0A0D1DUI7_USTMA Kinesin motor domain-containing protein OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=UMAG_04524 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 8.88KK8 pKa = 8.94RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 8.14RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.51MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6788

18

6806

4006347

20

5431

588.6

64.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.559 ± 0.028

1.068 ± 0.009

5.796 ± 0.021

5.344 ± 0.025

3.236 ± 0.017

6.444 ± 0.029

2.627 ± 0.014

4.164 ± 0.02

4.404 ± 0.024

8.967 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.926 ± 0.009

3.364 ± 0.015

5.864 ± 0.028

4.44 ± 0.02

6.376 ± 0.022

10.1 ± 0.043

6.01 ± 0.015

5.867 ± 0.02

1.187 ± 0.009

2.255 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski