Sewage-associated circular DNA virus-17

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGL4|A0A0B4UGL4_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-17 OX=1592084 PE=4 SV=1
MM1 pKa = 7.52TPTPKK6 pKa = 10.13KK7 pKa = 10.44SYY9 pKa = 10.84CFTLNNYY16 pKa = 8.54TEE18 pKa = 5.12DD19 pKa = 3.29EE20 pKa = 4.15LAAIRR25 pKa = 11.84GVCSTLAQYY34 pKa = 11.0AIVGRR39 pKa = 11.84EE40 pKa = 3.8VGEE43 pKa = 3.74QGTRR47 pKa = 11.84HH48 pKa = 4.85LQGYY52 pKa = 9.51IRR54 pKa = 11.84FTRR57 pKa = 11.84AYY59 pKa = 9.56RR60 pKa = 11.84FPTIKK65 pKa = 10.27DD66 pKa = 3.12RR67 pKa = 11.84YY68 pKa = 9.88LPRR71 pKa = 11.84CHH73 pKa = 7.59IEE75 pKa = 3.81VSRR78 pKa = 11.84GSPRR82 pKa = 11.84QNRR85 pKa = 11.84DD86 pKa = 2.93YY87 pKa = 10.87CSKK90 pKa = 10.9DD91 pKa = 3.31GEE93 pKa = 4.01FDD95 pKa = 3.45EE96 pKa = 5.47YY97 pKa = 11.79GEE99 pKa = 4.33LPEE102 pKa = 5.82DD103 pKa = 3.63RR104 pKa = 11.84ASSSRR109 pKa = 11.84EE110 pKa = 3.43DD111 pKa = 3.5LARR114 pKa = 11.84EE115 pKa = 4.38FARR118 pKa = 11.84DD119 pKa = 3.46ATDD122 pKa = 2.78GRR124 pKa = 11.84GGVRR128 pKa = 11.84RR129 pKa = 11.84FAEE132 pKa = 4.61SNPGTYY138 pKa = 10.07YY139 pKa = 10.86FSGHH143 pKa = 5.65NLLRR147 pKa = 11.84NHH149 pKa = 7.26WALQAPTARR158 pKa = 11.84PGITVDD164 pKa = 3.22WMHH167 pKa = 6.83GLPGVGKK174 pKa = 9.61SRR176 pKa = 11.84RR177 pKa = 11.84AHH179 pKa = 5.87EE180 pKa = 4.65RR181 pKa = 11.84LPDD184 pKa = 3.85AYY186 pKa = 9.91IKK188 pKa = 10.49DD189 pKa = 4.08PRR191 pKa = 11.84TKK193 pKa = 8.56WWNGYY198 pKa = 9.2FGEE201 pKa = 4.6KK202 pKa = 9.89EE203 pKa = 4.18VIIDD207 pKa = 3.68DD208 pKa = 4.93FGPQGIDD215 pKa = 2.87INHH218 pKa = 7.3LLRR221 pKa = 11.84WFDD224 pKa = 3.52RR225 pKa = 11.84YY226 pKa = 10.96KK227 pKa = 10.58CTVEE231 pKa = 4.05YY232 pKa = 10.09KK233 pKa = 10.64GGMMPLMAEE242 pKa = 4.19TFIVTSNYY250 pKa = 9.37HH251 pKa = 5.4PRR253 pKa = 11.84EE254 pKa = 3.86VFQTGRR260 pKa = 11.84STDD263 pKa = 3.48QQDD266 pKa = 3.07NTMYY270 pKa = 10.95DD271 pKa = 3.71HH272 pKa = 7.14PQLPALMRR280 pKa = 11.84RR281 pKa = 11.84INIIHH286 pKa = 5.5MQYY289 pKa = 9.7IIEE292 pKa = 4.38YY293 pKa = 9.97ISRR296 pKa = 11.84CMDD299 pKa = 3.44ACNEE303 pKa = 3.52ASEE306 pKa = 4.3IVKK309 pKa = 10.17EE310 pKa = 4.08IIKK313 pKa = 10.34KK314 pKa = 9.41IMRR317 pKa = 11.84RR318 pKa = 11.84VPAPGAEE325 pKa = 4.0IAA327 pKa = 4.37

Molecular weight: 37.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGL4|A0A0B4UGL4_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-17 OX=1592084 PE=4 SV=1
MM1 pKa = 7.35AFKK4 pKa = 10.42RR5 pKa = 11.84KK6 pKa = 9.77RR7 pKa = 11.84IMARR11 pKa = 11.84RR12 pKa = 11.84SGFKK16 pKa = 9.8KK17 pKa = 10.05RR18 pKa = 11.84RR19 pKa = 11.84ISRR22 pKa = 11.84KK23 pKa = 9.26SRR25 pKa = 11.84FARR28 pKa = 11.84KK29 pKa = 9.37KK30 pKa = 9.01RR31 pKa = 11.84GSGTRR36 pKa = 11.84SWTQGGSRR44 pKa = 11.84AMDD47 pKa = 3.04TRR49 pKa = 11.84FRR51 pKa = 11.84SRR53 pKa = 11.84KK54 pKa = 9.13LLNSGWRR61 pKa = 11.84RR62 pKa = 11.84HH63 pKa = 5.39LWGQTLHH70 pKa = 6.98LSPSRR75 pKa = 11.84SALPVPIVQTSGTILGKK92 pKa = 10.8GSAVLYY98 pKa = 9.98WPTFNNAAPSAGNAFWTTAGGLNEE122 pKa = 4.64LDD124 pKa = 4.11AGVTAPTFTSEE135 pKa = 3.71NLIIRR140 pKa = 11.84GGRR143 pKa = 11.84IGITLTQLGSVSDD156 pKa = 4.71DD157 pKa = 2.95IGITVYY163 pKa = 10.08TLAMAKK169 pKa = 8.33NTATGRR175 pKa = 11.84LVTPVPWGVSIDD187 pKa = 3.84SAPDD191 pKa = 3.26FAEE194 pKa = 4.2LGKK197 pKa = 10.42ILDD200 pKa = 3.9RR201 pKa = 11.84KK202 pKa = 10.4DD203 pKa = 3.98YY204 pKa = 11.45IMDD207 pKa = 4.0ANFRR211 pKa = 11.84AVTIEE216 pKa = 4.83RR217 pKa = 11.84RR218 pKa = 11.84LKK220 pKa = 9.51CQKK223 pKa = 10.21IDD225 pKa = 3.29MDD227 pKa = 3.7QYY229 pKa = 11.19SIQGGQQIAYY239 pKa = 9.74CVVTTNLTSTTNVTTNFLIYY259 pKa = 10.21HH260 pKa = 6.89DD261 pKa = 5.25LSFSNTAA268 pKa = 3.46

Molecular weight: 29.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

595

268

327

297.5

33.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.395 ± 0.459

1.513 ± 0.432

5.378 ± 0.508

4.538 ± 1.718

3.866 ± 0.135

8.235 ± 0.406

2.185 ± 0.601

6.891 ± 0.098

4.538 ± 0.387

6.387 ± 0.817

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.254

4.034 ± 0.25

4.706 ± 0.76

3.361 ± 0.002

9.748 ± 0.237

6.387 ± 1.238

7.899 ± 1.438

4.37 ± 0.271

1.849 ± 0.22

4.034 ± 1.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski