Trifolium-associated circular DNA virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4U8W1|A0A0B4U8W1_9VIRU Uncharacterized protein OS=Trifolium-associated circular DNA virus 1 OX=1590173 PE=4 SV=1
MM1 pKa = 7.84ARR3 pKa = 11.84QQHH6 pKa = 4.52QLEE9 pKa = 4.42RR10 pKa = 11.84SADD13 pKa = 3.68LQRR16 pKa = 11.84TNGTTTGSSQHH27 pKa = 6.66DD28 pKa = 3.95EE29 pKa = 4.09VRR31 pKa = 11.84GEE33 pKa = 3.98QTALRR38 pKa = 11.84SNDD41 pKa = 3.58SQHH44 pKa = 6.12VEE46 pKa = 3.91PGRR49 pKa = 11.84GHH51 pKa = 6.23NALRR55 pKa = 11.84LCDD58 pKa = 3.85EE59 pKa = 4.54VGCRR63 pKa = 11.84PEE65 pKa = 4.44PSTATEE71 pKa = 4.47PNPQSGGPAGGLEE84 pKa = 3.99EE85 pKa = 4.44RR86 pKa = 11.84SGGGEE91 pKa = 4.0DD92 pKa = 3.74SRR94 pKa = 11.84GRR96 pKa = 11.84LPGSDD101 pKa = 3.75YY102 pKa = 11.23DD103 pKa = 3.6QQRR106 pKa = 11.84WHH108 pKa = 6.91HH109 pKa = 6.1PVPLVSILQALQSCEE124 pKa = 3.77GHH126 pKa = 5.93SQDD129 pKa = 4.0TLVGGSTPPPCYY141 pKa = 10.1SPPSEE146 pKa = 4.17HH147 pKa = 6.22VRR149 pKa = 11.84HH150 pKa = 6.15TGGQDD155 pKa = 3.25RR156 pKa = 11.84EE157 pKa = 4.07HH158 pKa = 6.73SRR160 pKa = 11.84YY161 pKa = 9.87YY162 pKa = 11.02ADD164 pKa = 3.13QLEE167 pKa = 4.56YY168 pKa = 10.79QRR170 pKa = 11.84DD171 pKa = 3.17IHH173 pKa = 6.11PWINGLYY180 pKa = 8.83DD181 pKa = 3.33TGSQWEE187 pKa = 4.01YY188 pKa = 9.6RR189 pKa = 11.84QQSGRR194 pKa = 11.84GDD196 pKa = 3.59QQSDD200 pKa = 3.73RR201 pKa = 11.84DD202 pKa = 3.81DD203 pKa = 4.02SSCFGCSYY211 pKa = 11.38KK212 pKa = 10.57DD213 pKa = 3.08ICILFSVHH221 pKa = 6.33SGASPTRR228 pKa = 11.84QLL230 pKa = 3.75

Molecular weight: 25.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4U8Q6|A0A0B4U8Q6_9VIRU Replication-associated protein OS=Trifolium-associated circular DNA virus 1 OX=1590173 PE=3 SV=1
MM1 pKa = 7.78AYY3 pKa = 9.81KK4 pKa = 10.62LAGRR8 pKa = 11.84AAGATLGFIYY18 pKa = 10.69NNTKK22 pKa = 10.2GALIGARR29 pKa = 11.84MAKK32 pKa = 9.46YY33 pKa = 10.16ARR35 pKa = 11.84TGIRR39 pKa = 11.84ARR41 pKa = 11.84VARR44 pKa = 11.84SNKK47 pKa = 9.58RR48 pKa = 11.84KK49 pKa = 7.66ATVYY53 pKa = 10.64AGTKK57 pKa = 9.68RR58 pKa = 11.84RR59 pKa = 11.84GSVLAAPPAKK69 pKa = 9.85RR70 pKa = 11.84RR71 pKa = 11.84VFKK74 pKa = 10.83SRR76 pKa = 11.84PRR78 pKa = 11.84KK79 pKa = 7.81QLRR82 pKa = 11.84GGKK85 pKa = 9.25RR86 pKa = 11.84RR87 pKa = 11.84GGGSKK92 pKa = 10.27RR93 pKa = 11.84SSFANAIAKK102 pKa = 8.73TGASNFSIGSGAKK115 pKa = 9.69LYY117 pKa = 11.03SGFKK121 pKa = 9.24RR122 pKa = 11.84TQLPTTFNCTVTQRR136 pKa = 11.84VSGTSSTQSVVLLPTYY152 pKa = 10.11GLRR155 pKa = 11.84ATGEE159 pKa = 4.24TGDD162 pKa = 4.27TASQSQFTIDD172 pKa = 4.83KK173 pKa = 10.26SLLDD177 pKa = 3.58TAEE180 pKa = 3.73RR181 pKa = 11.84WLANNTNSSAAQTYY195 pKa = 9.99SGPTGRR201 pKa = 11.84RR202 pKa = 11.84QGVRR206 pKa = 11.84NTMKK210 pKa = 10.32FVVNKK215 pKa = 9.72LHH217 pKa = 6.26YY218 pKa = 10.5DD219 pKa = 3.33QMIRR223 pKa = 11.84NMSNQDD229 pKa = 3.18ADD231 pKa = 3.33ITLYY235 pKa = 10.97DD236 pKa = 3.56CVMRR240 pKa = 11.84SGVDD244 pKa = 3.39PNQAPQLSPIPNQADD259 pKa = 3.6PLADD263 pKa = 3.01WRR265 pKa = 11.84NGLEE269 pKa = 4.06VEE271 pKa = 4.91RR272 pKa = 11.84IAAGAYY278 pKa = 10.2LGATTINSVGTTPFHH293 pKa = 7.44SSAFCKK299 pKa = 10.22LYY301 pKa = 10.52RR302 pKa = 11.84VAKK305 pKa = 7.6VTRR308 pKa = 11.84KK309 pKa = 7.32TLSSGEE315 pKa = 4.18VHH317 pKa = 6.01HH318 pKa = 6.53HH319 pKa = 6.04HH320 pKa = 5.78VTVRR324 pKa = 11.84PRR326 pKa = 11.84NMFDD330 pKa = 3.26TQVDD334 pKa = 4.12KK335 pKa = 11.09IGNIPDD341 pKa = 3.29ITQTNLNTRR350 pKa = 11.84GTYY353 pKa = 9.76IPGLTGFTILVASGSIGNNPDD374 pKa = 2.85VATNNQIGMTAVALDD389 pKa = 3.91VVTKK393 pKa = 9.43TSASFSQFTRR403 pKa = 11.84EE404 pKa = 3.8RR405 pKa = 11.84RR406 pKa = 11.84QHH408 pKa = 5.76VNFDD412 pKa = 3.48GMSTNNITAVVLDD425 pKa = 4.24DD426 pKa = 3.67TDD428 pKa = 6.13AITTVQAAGGGVV440 pKa = 3.02

Molecular weight: 47.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1025

230

440

341.7

37.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.902 ± 1.453

2.146 ± 0.808

6.146 ± 1.001

3.902 ± 1.368

3.61 ± 0.949

8.098 ± 1.561

3.122 ± 0.845

4.39 ± 0.667

4.39 ± 1.125

6.341 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.366 ± 0.375

5.171 ± 0.868

4.78 ± 0.702

5.073 ± 1.269

7.415 ± 0.774

7.415 ± 1.357

7.902 ± 1.622

6.341 ± 0.792

1.561 ± 0.737

2.927 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski