Amino acid dipepetide frequency for bacterium HR30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.489AlaAla: 13.489 ± 0.128	1.398AlaCys: 1.398 ± 0.046	4.711AlaAsp: 4.711 ± 0.08	7.219AlaGlu: 7.219 ± 0.103	3.969AlaPhe: 3.969 ± 0.064	8.586AlaGly: 8.586 ± 0.101	2.279AlaHis: 2.279 ± 0.048	4.517AlaIle: 4.517 ± 0.074	3.02AlaLys: 3.02 ± 0.066	12.461AlaLeu: 12.461 ± 0.165	2.178AlaMet: 2.178 ± 0.052	2.58AlaAsn: 2.58 ± 0.062	5.061AlaPro: 5.061 ± 0.074	4.027AlaGln: 4.027 ± 0.07	9.334AlaArg: 9.334 ± 0.101	5.758AlaSer: 5.758 ± 0.081	6.057AlaThr: 6.057 ± 0.116	9.394AlaVal: 9.394 ± 0.114	1.839AlaTrp: 1.839 ± 0.047	2.444AlaTyr: 2.444 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.273CysAla: 1.273 ± 0.038	0.23CysCys: 0.23 ± 0.017	0.687CysAsp: 0.687 ± 0.029	0.651CysGlu: 0.651 ± 0.026	0.485CysPhe: 0.485 ± 0.023	1.354CysGly: 1.354 ± 0.039	0.407CysHis: 0.407 ± 0.03	0.433CysIle: 0.433 ± 0.022	0.278CysLys: 0.278 ± 0.017	1.114CysLeu: 1.114 ± 0.03	0.2CysMet: 0.2 ± 0.014	0.332CysAsn: 0.332 ± 0.021	0.876CysPro: 0.876 ± 0.041	0.423CysGln: 0.423 ± 0.023	1.067CysArg: 1.067 ± 0.034	0.814CysSer: 0.814 ± 0.034	0.783CysThr: 0.783 ± 0.036	1.065CysVal: 1.065 ± 0.038	0.198CysTrp: 0.198 ± 0.016	0.236CysTyr: 0.236 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.711AspAla: 4.711 ± 0.077	0.653AspCys: 0.653 ± 0.033	2.063AspAsp: 2.063 ± 0.054	2.994AspGlu: 2.994 ± 0.061	2.011AspPhe: 2.011 ± 0.041	4.291AspGly: 4.291 ± 0.082	1.049AspHis: 1.049 ± 0.028	1.881AspIle: 1.881 ± 0.043	0.917AspLys: 0.917 ± 0.031	5.181AspLeu: 5.181 ± 0.081	0.688AspMet: 0.688 ± 0.026	0.952AspAsn: 0.952 ± 0.03	3.787AspPro: 3.787 ± 0.073	1.402AspGln: 1.402 ± 0.037	3.944AspArg: 3.944 ± 0.065	1.908AspSer: 1.908 ± 0.046	2.152AspThr: 2.152 ± 0.048	3.922AspVal: 3.922 ± 0.073	0.827AspTrp: 0.827 ± 0.026	1.173AspTyr: 1.173 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.141GluAla: 7.141 ± 0.097	0.627GluCys: 0.627 ± 0.027	2.549GluAsp: 2.549 ± 0.047	4.816GluGlu: 4.816 ± 0.096	2.241GluPhe: 2.241 ± 0.042	4.345GluGly: 4.345 ± 0.07	1.513GluHis: 1.513 ± 0.041	3.215GluIle: 3.215 ± 0.062	2.1GluLys: 2.1 ± 0.052	7.348GluLeu: 7.348 ± 0.098	1.2GluMet: 1.2 ± 0.04	1.467GluAsn: 1.467 ± 0.039	3.354GluPro: 3.354 ± 0.062	2.904GluGln: 2.904 ± 0.055	6.577GluArg: 6.577 ± 0.107	2.737GluSer: 2.737 ± 0.053	2.678GluThr: 2.678 ± 0.049	5.415GluVal: 5.415 ± 0.084	0.876GluTrp: 0.876 ± 0.03	1.318GluTyr: 1.318 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.338PheAla: 4.338 ± 0.075	0.615PheCys: 0.615 ± 0.025	2.255PheAsp: 2.255 ± 0.054	2.215PheGlu: 2.215 ± 0.044	1.75PhePhe: 1.75 ± 0.051	3.562PheGly: 3.562 ± 0.068	0.938PheHis: 0.938 ± 0.03	1.142PheIle: 1.142 ± 0.034	0.632PheLys: 0.632 ± 0.026	4.207PheLeu: 4.207 ± 0.081	0.451PheMet: 0.451 ± 0.02	0.825PheAsn: 0.825 ± 0.034	2.1PhePro: 2.1 ± 0.038	1.145PheGln: 1.145 ± 0.034	2.996PheArg: 2.996 ± 0.051	2.165PheSer: 2.165 ± 0.054	2.059PheThr: 2.059 ± 0.055	3.446PheVal: 3.446 ± 0.058	0.624PheTrp: 0.624 ± 0.027	1.02PheTyr: 1.02 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	8.025GlyAla: 8.025 ± 0.096	1.261GlyCys: 1.261 ± 0.038	3.913GlyAsp: 3.913 ± 0.08	5.003GlyGlu: 5.003 ± 0.073	3.328GlyPhe: 3.328 ± 0.061	6.918GlyGly: 6.918 ± 0.115	1.7GlyHis: 1.7 ± 0.039	3.858GlyIle: 3.858 ± 0.067	2.814GlyLys: 2.814 ± 0.059	7.971GlyLeu: 7.971 ± 0.097	1.691GlyMet: 1.691 ± 0.046	2.327GlyAsn: 2.327 ± 0.058	3.699GlyPro: 3.699 ± 0.064	2.942GlyGln: 2.942 ± 0.056	6.346GlyArg: 6.346 ± 0.087	4.928GlySer: 4.928 ± 0.097	4.702GlyThr: 4.702 ± 0.084	7.195GlyVal: 7.195 ± 0.116	1.517GlyTrp: 1.517 ± 0.043	2.09GlyTyr: 2.09 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.268HisAla: 2.268 ± 0.055	0.371HisCys: 0.371 ± 0.018	1.01HisAsp: 1.01 ± 0.027	1.287HisGlu: 1.287 ± 0.034	0.94HisPhe: 0.94 ± 0.028	2.012HisGly: 2.012 ± 0.045	0.601HisHis: 0.601 ± 0.025	0.808HisIle: 0.808 ± 0.03	0.424HisLys: 0.424 ± 0.019	2.346HisLeu: 2.346 ± 0.052	0.369HisMet: 0.369 ± 0.018	0.479HisAsn: 0.479 ± 0.021	1.618HisPro: 1.618 ± 0.039	0.632HisGln: 0.632 ± 0.026	1.907HisArg: 1.907 ± 0.051	1.015HisSer: 1.015 ± 0.029	1.049HisThr: 1.049 ± 0.033	1.781HisVal: 1.781 ± 0.038	0.444HisTrp: 0.444 ± 0.021	0.606HisTyr: 0.606 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	4.867IleAla: 4.867 ± 0.069	0.476IleCys: 0.476 ± 0.021	2.55IleAsp: 2.55 ± 0.056	3.09IleGlu: 3.09 ± 0.054	1.441IlePhe: 1.441 ± 0.033	3.425IleGly: 3.425 ± 0.068	0.896IleHis: 0.896 ± 0.032	1.337IleIle: 1.337 ± 0.04	0.844IleLys: 0.844 ± 0.031	3.947IleLeu: 3.947 ± 0.07	0.482IleMet: 0.482 ± 0.021	0.861IleAsn: 0.861 ± 0.032	2.379IlePro: 2.379 ± 0.052	1.288IleGln: 1.288 ± 0.031	3.315IleArg: 3.315 ± 0.06	1.968IleSer: 1.968 ± 0.048	1.946IleThr: 1.946 ± 0.041	4.051IleVal: 4.051 ± 0.067	0.474IleTrp: 0.474 ± 0.022	0.878IleTyr: 0.878 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	2.685LysAla: 2.685 ± 0.061	0.21LysCys: 0.21 ± 0.013	1.069LysAsp: 1.069 ± 0.036	1.779LysGlu: 1.779 ± 0.048	0.785LysPhe: 0.785 ± 0.029	1.949LysGly: 1.949 ± 0.046	0.566LysHis: 0.566 ± 0.024	1.32LysIle: 1.32 ± 0.039	0.925LysLys: 0.925 ± 0.037	2.828LysLeu: 2.828 ± 0.066	0.521LysMet: 0.521 ± 0.024	0.686LysAsn: 0.686 ± 0.024	1.655LysPro: 1.655 ± 0.045	1.019LysGln: 1.019 ± 0.035	2.23LysArg: 2.23 ± 0.049	1.161LysSer: 1.161 ± 0.033	1.269LysThr: 1.269 ± 0.035	2.182LysVal: 2.182 ± 0.05	0.319LysTrp: 0.319 ± 0.016	0.575LysTyr: 0.575 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.139LeuAla: 13.139 ± 0.142	1.312LeuCys: 1.312 ± 0.038	5.105LeuAsp: 5.105 ± 0.074	7.064LeuGlu: 7.064 ± 0.097	3.957LeuPhe: 3.957 ± 0.068	9.036LeuGly: 9.036 ± 0.114	2.255LeuHis: 2.255 ± 0.044	3.464LeuIle: 3.464 ± 0.068	2.423LeuLys: 2.423 ± 0.048	11.339LeuLeu: 11.339 ± 0.164	1.488LeuMet: 1.488 ± 0.041	2.074LeuAsn: 2.074 ± 0.041	6.574LeuPro: 6.574 ± 0.078	3.771LeuGln: 3.771 ± 0.067	9.69LeuArg: 9.69 ± 0.121	5.596LeuSer: 5.596 ± 0.082	4.948LeuThr: 4.948 ± 0.073	8.921LeuVal: 8.921 ± 0.096	1.689LeuTrp: 1.689 ± 0.053	2.386LeuTyr: 2.386 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.008MetAla: 2.008 ± 0.047	0.157MetCys: 0.157 ± 0.011	0.652MetAsp: 0.652 ± 0.026	1.047MetGlu: 1.047 ± 0.034	0.555MetPhe: 0.555 ± 0.023	1.309MetGly: 1.309 ± 0.035	0.355MetHis: 0.355 ± 0.021	0.751MetIle: 0.751 ± 0.028	0.63MetLys: 0.63 ± 0.026	1.688MetLeu: 1.688 ± 0.05	0.356MetMet: 0.356 ± 0.018	0.518MetAsn: 0.518 ± 0.021	1.058MetPro: 1.058 ± 0.035	0.603MetGln: 0.603 ± 0.023	1.558MetArg: 1.558 ± 0.041	0.967MetSer: 0.967 ± 0.032	1.022MetThr: 1.022 ± 0.032	1.409MetVal: 1.409 ± 0.038	0.209MetTrp: 0.209 ± 0.015	0.296MetTyr: 0.296 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.511AsnAla: 2.511 ± 0.043	0.348AsnCys: 0.348 ± 0.022	1.183AsnAsp: 1.183 ± 0.039	1.304AsnGlu: 1.304 ± 0.032	0.983AsnPhe: 0.983 ± 0.031	2.204AsnGly: 2.204 ± 0.072	0.477AsnHis: 0.477 ± 0.023	1.015AsnIle: 1.015 ± 0.034	0.505AsnLys: 0.505 ± 0.024	2.578AsnLeu: 2.578 ± 0.044	0.363AsnMet: 0.363 ± 0.02	0.668AsnAsn: 0.668 ± 0.031	1.958AsnPro: 1.958 ± 0.052	0.81AsnGln: 0.81 ± 0.032	1.875AsnArg: 1.875 ± 0.041	1.152AsnSer: 1.152 ± 0.056	1.174AsnThr: 1.174 ± 0.04	2.075AsnVal: 2.075 ± 0.05	0.38AsnTrp: 0.38 ± 0.023	0.581AsnTyr: 0.581 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	5.7ProAla: 5.7 ± 0.069	0.653ProCys: 0.653 ± 0.025	2.953ProAsp: 2.953 ± 0.056	4.386ProGlu: 4.386 ± 0.079	2.219ProPhe: 2.219 ± 0.05	4.829ProGly: 4.829 ± 0.081	1.307ProHis: 1.307 ± 0.039	2.136ProIle: 2.136 ± 0.039	1.519ProLys: 1.519 ± 0.048	5.855ProLeu: 5.855 ± 0.081	1.115ProMet: 1.115 ± 0.031	1.596ProAsn: 1.596 ± 0.046	3.957ProPro: 3.957 ± 0.084	2.086ProGln: 2.086 ± 0.045	4.416ProArg: 4.416 ± 0.073	3.535ProSer: 3.535 ± 0.085	4.006ProThr: 4.006 ± 0.16	4.672ProVal: 4.672 ± 0.068	1.014ProTrp: 1.014 ± 0.032	1.3ProTyr: 1.3 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	4.147GlnAla: 4.147 ± 0.069	0.482GlnCys: 0.482 ± 0.022	1.254GlnAsp: 1.254 ± 0.035	2.086GlnGlu: 2.086 ± 0.045	1.166GlnPhe: 1.166 ± 0.032	2.64GlnGly: 2.64 ± 0.047	0.823GlnHis: 0.823 ± 0.028	1.542GlnIle: 1.542 ± 0.037	1.031GlnLys: 1.031 ± 0.033	3.798GlnLeu: 3.798 ± 0.066	0.654GlnMet: 0.654 ± 0.023	0.841GlnAsn: 0.841 ± 0.028	2.026GlnPro: 2.026 ± 0.045	1.624GlnGln: 1.624 ± 0.043	3.886GlnArg: 3.886 ± 0.069	1.684GlnSer: 1.684 ± 0.04	1.579GlnThr: 1.579 ± 0.038	2.932GlnVal: 2.932 ± 0.052	0.679GlnTrp: 0.679 ± 0.029	0.762GlnTyr: 0.762 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	8.984ArgAla: 8.984 ± 0.106	1.117ArgCys: 1.117 ± 0.04	3.819ArgAsp: 3.819 ± 0.061	5.978ArgGlu: 5.978 ± 0.088	3.463ArgPhe: 3.463 ± 0.061	6.081ArgGly: 6.081 ± 0.078	1.917ArgHis: 1.917 ± 0.042	3.817ArgIle: 3.817 ± 0.058	2.426ArgLys: 2.426 ± 0.052	9.07ArgLeu: 9.07 ± 0.111	1.698ArgMet: 1.698 ± 0.043	2.161ArgAsn: 2.161 ± 0.039	4.354ArgPro: 4.354 ± 0.071	3.436ArgGln: 3.436 ± 0.061	7.558ArgArg: 7.558 ± 0.108	4.438ArgSer: 4.438 ± 0.071	4.075ArgThr: 4.075 ± 0.071	7.084ArgVal: 7.084 ± 0.087	1.725ArgTrp: 1.725 ± 0.043	2.322ArgTyr: 2.322 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	5.452SerAla: 5.452 ± 0.084	0.72SerCys: 0.72 ± 0.034	2.399SerAsp: 2.399 ± 0.054	2.915SerGlu: 2.915 ± 0.053	2.295SerPhe: 2.295 ± 0.045	4.966SerGly: 4.966 ± 0.088	1.026SerHis: 1.026 ± 0.031	1.982SerIle: 1.982 ± 0.042	1.131SerLys: 1.131 ± 0.035	5.518SerLeu: 5.518 ± 0.086	0.965SerMet: 0.965 ± 0.031	1.267SerAsn: 1.267 ± 0.038	3.654SerPro: 3.654 ± 0.071	1.667SerGln: 1.667 ± 0.038	3.856SerArg: 3.856 ± 0.062	3.069SerSer: 3.069 ± 0.073	2.787SerThr: 2.787 ± 0.064	4.172SerVal: 4.172 ± 0.074	0.911SerTrp: 0.911 ± 0.031	1.163SerTyr: 1.163 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	5.562ThrAla: 5.562 ± 0.11	0.622ThrCys: 0.622 ± 0.029	2.2ThrAsp: 2.2 ± 0.053	2.679ThrGlu: 2.679 ± 0.054	1.959ThrPhe: 1.959 ± 0.053	4.508ThrGly: 4.508 ± 0.069	1.089ThrHis: 1.089 ± 0.032	2.438ThrIle: 2.438 ± 0.06	1.264ThrLys: 1.264 ± 0.037	5.486ThrLeu: 5.486 ± 0.09	0.911ThrMet: 0.911 ± 0.028	1.465ThrAsn: 1.465 ± 0.056	4.056ThrPro: 4.056 ± 0.165	1.545ThrGln: 1.545 ± 0.039	3.556ThrArg: 3.556 ± 0.061	2.596ThrSer: 2.596 ± 0.059	2.828ThrThr: 2.828 ± 0.064	4.647ThrVal: 4.647 ± 0.093	0.769ThrTrp: 0.769 ± 0.03	1.209ThrTyr: 1.209 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	10.25ValAla: 10.25 ± 0.114	1.107ValCys: 1.107 ± 0.035	4.233ValAsp: 4.233 ± 0.071	5.559ValGlu: 5.559 ± 0.078	3.158ValPhe: 3.158 ± 0.06	7.011ValGly: 7.011 ± 0.133	1.801ValHis: 1.801 ± 0.039	3.389ValIle: 3.389 ± 0.069	1.863ValLys: 1.863 ± 0.046	8.787ValLeu: 8.787 ± 0.105	1.179ValMet: 1.179 ± 0.037	1.985ValAsn: 1.985 ± 0.055	4.908ValPro: 4.908 ± 0.063	2.726ValGln: 2.726 ± 0.05	7.59ValArg: 7.59 ± 0.089	4.451ValSer: 4.451 ± 0.085	4.593ValThr: 4.593 ± 0.098	8.261ValVal: 8.261 ± 0.116	1.3ValTrp: 1.3 ± 0.04	1.743ValTyr: 1.743 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.449TrpAla: 1.449 ± 0.04	0.231TrpCys: 0.231 ± 0.016	0.663TrpAsp: 0.663 ± 0.026	0.932TrpGlu: 0.932 ± 0.029	0.7TrpPhe: 0.7 ± 0.028	1.231TrpGly: 1.231 ± 0.042	0.388TrpHis: 0.388 ± 0.021	0.746TrpIle: 0.746 ± 0.032	0.5TrpLys: 0.5 ± 0.02	2.102TrpLeu: 2.102 ± 0.058	0.346TrpMet: 0.346 ± 0.02	0.515TrpAsn: 0.515 ± 0.025	0.821TrpPro: 0.821 ± 0.03	0.778TrpGln: 0.778 ± 0.027	1.539TrpArg: 1.539 ± 0.044	0.927TrpSer: 0.927 ± 0.027	0.678TrpThr: 0.678 ± 0.025	1.326TrpVal: 1.326 ± 0.041	0.421TrpTrp: 0.421 ± 0.024	0.355TrpTyr: 0.355 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.323TyrAla: 2.323 ± 0.053	0.316TyrCys: 0.316 ± 0.018	1.238TyrAsp: 1.238 ± 0.041	1.418TyrGlu: 1.418 ± 0.042	1.081TyrPhe: 1.081 ± 0.038	1.972TyrGly: 1.972 ± 0.046	0.581TyrHis: 0.581 ± 0.021	0.725TyrIle: 0.725 ± 0.029	0.487TyrLys: 0.487 ± 0.022	2.61TyrLeu: 2.61 ± 0.057	0.291TyrMet: 0.291 ± 0.018	0.544TyrAsn: 0.544 ± 0.024	1.354TyrPro: 1.354 ± 0.042	0.806TyrGln: 0.806 ± 0.029	2.281TyrArg: 2.281 ± 0.054	1.047TyrSer: 1.047 ± 0.034	1.003TyrThr: 1.003 ± 0.037	1.944TyrVal: 1.944 ± 0.045	0.436TyrTrp: 0.436 ± 0.022	0.664TyrTyr: 0.664 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3301 proteins (1077378 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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