Tortoise microvirus 36

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W9M6|A0A4P8W9M6_9VIRU Uncharacterized protein OS=Tortoise microvirus 36 OX=2583139 PE=4 SV=1
MM1 pKa = 6.89FTFHH5 pKa = 7.01SFLHH9 pKa = 6.01FLCFYY14 pKa = 10.59IGVCMFDD21 pKa = 3.41VDD23 pKa = 3.97TSDD26 pKa = 5.14GSILPSGRR34 pKa = 11.84SRR36 pKa = 11.84AVDD39 pKa = 3.35VTILFPEE46 pKa = 4.27SFIDD50 pKa = 5.75AIDD53 pKa = 3.75NLLLGDD59 pKa = 4.09TMSLPDD65 pKa = 5.63FIRR68 pKa = 11.84LACVEE73 pKa = 4.19YY74 pKa = 10.15FISTLMCC81 pKa = 4.61

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W620|A0A4P8W620_9VIRU DNA pilot protein OS=Tortoise microvirus 36 OX=2583139 PE=4 SV=1
MM1 pKa = 7.56SCRR4 pKa = 11.84ITYY7 pKa = 9.91QSYY10 pKa = 11.24LLFCCKK16 pKa = 10.02RR17 pKa = 11.84EE18 pKa = 4.95LFDD21 pKa = 4.33CYY23 pKa = 10.61QRR25 pKa = 11.84GLGASFLTFTYY36 pKa = 11.05NDD38 pKa = 3.93DD39 pKa = 3.89CLPLYY44 pKa = 10.82GSLDD48 pKa = 3.2KK49 pKa = 11.17RR50 pKa = 11.84SFQNLLKK57 pKa = 10.46RR58 pKa = 11.84ARR60 pKa = 11.84RR61 pKa = 11.84NKK63 pKa = 9.7GLPAFKK69 pKa = 10.61YY70 pKa = 8.99LACGEE75 pKa = 4.37YY76 pKa = 10.63GDD78 pKa = 4.28KK79 pKa = 10.29FDD81 pKa = 4.09RR82 pKa = 11.84AHH84 pKa = 5.26YY85 pKa = 9.48HH86 pKa = 6.17AVFFGLTDD94 pKa = 3.3VLAKK98 pKa = 10.51QFVASHH104 pKa = 6.58WLNGYY109 pKa = 9.47VKK111 pKa = 10.6VEE113 pKa = 4.1ALRR116 pKa = 11.84SSAGLRR122 pKa = 11.84YY123 pKa = 10.1VLDD126 pKa = 3.97YY127 pKa = 10.64CCKK130 pKa = 10.57SINGDD135 pKa = 3.91LAVEE139 pKa = 4.82LYY141 pKa = 10.52DD142 pKa = 3.31NQMRR146 pKa = 11.84EE147 pKa = 4.13RR148 pKa = 11.84PFLSHH153 pKa = 5.04STRR156 pKa = 11.84MGFDD160 pKa = 2.77WLVRR164 pKa = 11.84NMDD167 pKa = 4.17EE168 pKa = 3.87IVANGFYY175 pKa = 10.43YY176 pKa = 10.51RR177 pKa = 11.84EE178 pKa = 4.22AGKK181 pKa = 8.7MLPLPKK187 pKa = 9.96YY188 pKa = 10.41FRR190 pKa = 11.84DD191 pKa = 3.33IYY193 pKa = 10.45DD194 pKa = 3.37TYY196 pKa = 10.86KK197 pKa = 10.23TFDD200 pKa = 3.15VSRR203 pKa = 11.84VLRR206 pKa = 11.84SLDD209 pKa = 3.36SHH211 pKa = 6.91AKK213 pKa = 10.22RR214 pKa = 11.84MGFPDD219 pKa = 3.33IYY221 pKa = 10.71SYY223 pKa = 11.71SDD225 pKa = 3.31YY226 pKa = 10.84ATPLKK231 pKa = 10.41EE232 pKa = 4.36KK233 pKa = 10.63EE234 pKa = 4.44FISKK238 pKa = 10.14SRR240 pKa = 11.84LAGRR244 pKa = 11.84PVDD247 pKa = 3.85DD248 pKa = 4.7SNFVSSFYY256 pKa = 10.99VPRR259 pKa = 11.84LGTNNLAKK267 pKa = 10.55SLLEE271 pKa = 3.98RR272 pKa = 4.81

Molecular weight: 31.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1635

81

591

233.6

26.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.073 ± 1.343

1.774 ± 0.572

6.667 ± 1.091

4.587 ± 0.88

5.443 ± 0.911

5.26 ± 0.391

1.529 ± 0.237

5.199 ± 0.739

4.037 ± 1.029

9.235 ± 0.819

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.242

4.771 ± 0.35

4.404 ± 0.766

3.914 ± 1.694

5.627 ± 0.607

10.398 ± 1.03

5.749 ± 0.697

4.709 ± 0.401

0.856 ± 0.172

5.138 ± 0.687
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski