Puccinia striiformis f. sp. tritici PST-78

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia; Puccinia striiformis; Puccinia striiformis f. sp. tritici

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0VLM8|A0A0L0VLM8_9BASI Uncharacterized protein OS=Puccinia striiformis f. sp. tritici PST-78 OX=1165861 GN=PSTG_06757 PE=4 SV=1
MM1 pKa = 7.56LKK3 pKa = 9.65MKK5 pKa = 10.53VSLITITISLILLGTSVFASPSHH28 pKa = 6.48LGTRR32 pKa = 11.84TTSGRR37 pKa = 11.84RR38 pKa = 11.84HH39 pKa = 4.0THH41 pKa = 5.54IIARR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 7.17TGKK50 pKa = 10.64GMTRR54 pKa = 11.84RR55 pKa = 11.84DD56 pKa = 3.34GGDD59 pKa = 3.22YY60 pKa = 11.32GGDD63 pKa = 3.48NQPATDD69 pKa = 3.95SYY71 pKa = 11.44QPPEE75 pKa = 3.99TSDD78 pKa = 3.58YY79 pKa = 11.02QPVGTTQDD87 pKa = 3.54YY88 pKa = 11.13SSYY91 pKa = 11.48APDD94 pKa = 3.55SQDD97 pKa = 3.42YY98 pKa = 10.82KK99 pKa = 11.22SADD102 pKa = 3.4PSDD105 pKa = 4.61TYY107 pKa = 11.71DD108 pKa = 4.73KK109 pKa = 11.42DD110 pKa = 3.4PDD112 pKa = 3.76YY113 pKa = 11.44QPTGTANVTSTPPSDD128 pKa = 3.95GEE130 pKa = 5.11DD131 pKa = 3.71YY132 pKa = 11.36ASDD135 pKa = 4.01DD136 pKa = 3.45SDD138 pKa = 4.04YY139 pKa = 11.71AADD142 pKa = 4.3DD143 pKa = 3.89ADD145 pKa = 4.07YY146 pKa = 11.41APDD149 pKa = 4.45DD150 pKa = 3.96SDD152 pKa = 4.15YY153 pKa = 11.67APEE156 pKa = 4.99DD157 pKa = 3.3SDD159 pKa = 4.87YY160 pKa = 11.71APEE163 pKa = 4.98DD164 pKa = 3.63PDD166 pKa = 3.87YY167 pKa = 11.47APEE170 pKa = 4.88DD171 pKa = 3.47SDD173 pKa = 4.06YY174 pKa = 11.34QPTGTDD180 pKa = 3.99PDD182 pKa = 4.47PSTPAPDD189 pKa = 4.23GDD191 pKa = 4.99DD192 pKa = 3.6YY193 pKa = 12.14ASS195 pKa = 3.18

Molecular weight: 21.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0UJQ0|A0A0L0UJQ0_9BASI Uncharacterized protein (Fragment) OS=Puccinia striiformis f. sp. tritici PST-78 OX=1165861 GN=PSTG_19378 PE=4 SV=1
MM1 pKa = 7.49HH2 pKa = 7.26LHH4 pKa = 6.81ASWAAAGATGSRR16 pKa = 11.84TGGAGRR22 pKa = 11.84ATVRR26 pKa = 11.84HH27 pKa = 5.36RR28 pKa = 11.84QSPASMHH35 pKa = 6.46LHH37 pKa = 6.56ASWAAAGATGSRR49 pKa = 11.84TGGAGRR55 pKa = 11.84ATVRR59 pKa = 11.84HH60 pKa = 5.36RR61 pKa = 11.84QSPASMHH68 pKa = 6.46LHH70 pKa = 6.56ASWAAAGATGSRR82 pKa = 11.84TGGAGRR88 pKa = 11.84ATVRR92 pKa = 11.84HH93 pKa = 5.36RR94 pKa = 11.84QSPASMHH101 pKa = 6.46LHH103 pKa = 6.56ASWAAAGATGSRR115 pKa = 11.84TGGAGRR121 pKa = 11.84ATGLPTGSHH130 pKa = 6.09

Molecular weight: 12.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19368

564

19932

7397404

18

4479

371.1

41.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.793 ± 0.017

1.343 ± 0.008

5.551 ± 0.013

5.864 ± 0.02

3.597 ± 0.012

5.688 ± 0.019

2.872 ± 0.01

5.501 ± 0.015

5.698 ± 0.021

9.353 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.008

4.699 ± 0.012

6.33 ± 0.022

4.667 ± 0.014

5.52 ± 0.013

9.57 ± 0.027

6.205 ± 0.013

5.149 ± 0.012

1.231 ± 0.007

2.404 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski