Beet western yellows virus associated RNA

Taxonomy: Viruses; Riboviria; Virus-associated RNAs

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0P708|A0A0A0P708_9VIRU RNA-directed RNA polymerase OS=Beet western yellows virus associated RNA OX=1425367 PE=4 SV=1
MM1 pKa = 7.67FKK3 pKa = 10.66RR4 pKa = 11.84FTVSDD9 pKa = 3.72TPVCFKK15 pKa = 10.74GLNYY19 pKa = 10.02LRR21 pKa = 11.84RR22 pKa = 11.84GNFMKK27 pKa = 10.53EE28 pKa = 3.27KK29 pKa = 9.53WEE31 pKa = 4.29SFNDD35 pKa = 3.73PVAICLDD42 pKa = 3.45ASRR45 pKa = 11.84FDD47 pKa = 3.57LHH49 pKa = 8.19VSEE52 pKa = 6.09DD53 pKa = 4.03ALQHH57 pKa = 4.62THH59 pKa = 6.62LLYY62 pKa = 8.43MTAIGNDD69 pKa = 3.42PQLAYY74 pKa = 9.79ILEE77 pKa = 4.35NRR79 pKa = 11.84LVTDD83 pKa = 4.44GVGWSSDD90 pKa = 3.07GAFRR94 pKa = 11.84YY95 pKa = 8.82HH96 pKa = 7.1KK97 pKa = 10.64KK98 pKa = 10.54GGRR101 pKa = 11.84CSGDD105 pKa = 2.94NDD107 pKa = 3.61TSLGNVIIMLSITYY121 pKa = 8.74AFCEE125 pKa = 4.27EE126 pKa = 4.06SDD128 pKa = 3.54IPHH131 pKa = 6.82IEE133 pKa = 4.0VTNDD137 pKa = 2.82GDD139 pKa = 3.79DD140 pKa = 3.42QVIMVEE146 pKa = 4.21RR147 pKa = 11.84EE148 pKa = 3.84HH149 pKa = 6.13MHH151 pKa = 5.68TVLQIEE157 pKa = 4.22DD158 pKa = 3.8TFRR161 pKa = 11.84KK162 pKa = 9.75FGFLLKK168 pKa = 10.74VEE170 pKa = 4.4DD171 pKa = 3.93PVYY174 pKa = 9.98EE175 pKa = 3.96LEE177 pKa = 6.09RR178 pKa = 11.84IDD180 pKa = 4.18FCQTRR185 pKa = 11.84PVQLTPDD192 pKa = 3.24VCTMVRR198 pKa = 11.84HH199 pKa = 6.23PRR201 pKa = 11.84LAMTKK206 pKa = 10.27DD207 pKa = 3.25LTTFIPIEE215 pKa = 4.15RR216 pKa = 11.84GKK218 pKa = 10.76LKK220 pKa = 10.85YY221 pKa = 10.34HH222 pKa = 6.45MLTAIGKK229 pKa = 9.25CGSACYY235 pKa = 10.46DD236 pKa = 4.55DD237 pKa = 5.5IPVLRR242 pKa = 11.84AMYY245 pKa = 10.26RR246 pKa = 11.84KK247 pKa = 9.06MEE249 pKa = 4.84IIGTRR254 pKa = 11.84NKK256 pKa = 10.54GNEE259 pKa = 3.97DD260 pKa = 3.38SWWNSTHH267 pKa = 7.24ADD269 pKa = 3.41PAFRR273 pKa = 11.84TLSSKK278 pKa = 10.81AATNVGLTTQCRR290 pKa = 11.84ISFWKK295 pKa = 10.69AFGILPDD302 pKa = 3.57QQEE305 pKa = 4.02ALEE308 pKa = 4.25NEE310 pKa = 3.79WRR312 pKa = 11.84QWEE315 pKa = 4.06PSLDD319 pKa = 3.11RR320 pKa = 11.84VEE322 pKa = 5.0FVHH325 pKa = 6.78SFLGDD330 pKa = 3.57YY331 pKa = 7.89PVCEE335 pKa = 4.23GDD337 pKa = 3.51VV338 pKa = 3.25

Molecular weight: 38.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0P5P8|A0A0A0P5P8_9VIRU ORF4 OS=Beet western yellows virus associated RNA OX=1425367 PE=4 SV=1
MM1 pKa = 7.58SPDD4 pKa = 3.46EE5 pKa = 5.1FLACYY10 pKa = 9.97AGRR13 pKa = 11.84KK14 pKa = 5.92KK15 pKa = 9.25TIYY18 pKa = 10.62AKK20 pKa = 10.65AILSLAATPLNIRR33 pKa = 11.84DD34 pKa = 3.5FRR36 pKa = 11.84VRR38 pKa = 11.84AFIKK42 pKa = 10.47KK43 pKa = 9.88EE44 pKa = 3.64KK45 pKa = 10.62DD46 pKa = 2.99KK47 pKa = 11.24LLSSNSDD54 pKa = 3.05PRR56 pKa = 11.84IIQPRR61 pKa = 11.84HH62 pKa = 4.93PRR64 pKa = 11.84FLVSLGRR71 pKa = 11.84YY72 pKa = 8.38IRR74 pKa = 11.84PLEE77 pKa = 3.92QIVYY81 pKa = 10.3KK82 pKa = 9.49SLRR85 pKa = 11.84DD86 pKa = 3.73CSSDD90 pKa = 3.34SQSQIPPCASKK101 pKa = 11.11ASITSGGGILL111 pKa = 4.47

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

477

28

338

159.0

18.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.66 ± 1.008

3.145 ± 0.662

7.338 ± 0.847

5.241 ± 1.628

4.612 ± 1.024

5.66 ± 0.702

2.935 ± 0.957

6.289 ± 1.612

5.241 ± 1.294

8.386 ± 0.893

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.757

3.354 ± 0.661

5.031 ± 1.11

3.354 ± 0.7

7.338 ± 1.587

6.918 ± 2.35

6.709 ± 2.615

5.451 ± 1.326

1.468 ± 0.753

3.145 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski