Ramlibacter rhizophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Ramlibacter

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0BJX2|A0A4Z0BJX2_9BURK DNA polymerase III subunit epsilon OS=Ramlibacter rhizophilus OX=1781167 GN=dnaQ PE=4 SV=1
MM1 pKa = 7.22SAVAEE6 pKa = 4.42TTQPDD11 pKa = 3.57TGMPAPLLFTDD22 pKa = 4.0SAAAKK27 pKa = 10.02VADD30 pKa = 5.45LIAEE34 pKa = 4.31EE35 pKa = 4.82GNPDD39 pKa = 2.99LKK41 pKa = 11.13LRR43 pKa = 11.84VFVQGGGCSGFQYY56 pKa = 10.94GFTFDD61 pKa = 5.26EE62 pKa = 4.59IQNDD66 pKa = 4.06DD67 pKa = 3.99DD68 pKa = 4.19TVMSKK73 pKa = 10.86NGVSLLIDD81 pKa = 3.54AMSYY85 pKa = 10.57QYY87 pKa = 11.72LLGAEE92 pKa = 3.98IDD94 pKa = 4.02YY95 pKa = 11.34KK96 pKa = 11.22EE97 pKa = 4.67DD98 pKa = 3.23LQGAQFVIKK107 pKa = 10.4NPNATTTCGCGSSFSVV123 pKa = 3.54

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0BG16|A0A4Z0BG16_9BURK Uncharacterized protein OS=Ramlibacter rhizophilus OX=1781167 GN=EZ242_17585 PE=4 SV=1
MM1 pKa = 7.15VGAGGGVGAGAGAGGGGAGMGAGAGAGAGAGTATTGGGAGGASGGGALAQPATSAIRR58 pKa = 11.84GASTQATAVAGRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84GAQRR76 pKa = 11.84VGRR79 pKa = 11.84ARR81 pKa = 11.84GPTVSIRR88 pKa = 11.84MGG90 pKa = 3.17

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4252

0

4252

1393763

26

4473

327.8

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.397 ± 0.062

0.95 ± 0.013

5.048 ± 0.026

5.642 ± 0.028

3.383 ± 0.025

8.592 ± 0.042

2.306 ± 0.018

3.794 ± 0.026

2.576 ± 0.031

11.013 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.018

2.154 ± 0.019

5.734 ± 0.033

3.989 ± 0.026

7.989 ± 0.043

5.067 ± 0.029

4.73 ± 0.024

7.79 ± 0.033

1.536 ± 0.018

1.984 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski