Ntepes virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Ntepes phlebovirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499S7X0|A0A499S7X0_9VIRU Replicase OS=Ntepes virus OX=2569589 GN=RdRp PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 8.03MTTRR6 pKa = 11.84FLYY9 pKa = 10.54DD10 pKa = 3.15RR11 pKa = 11.84LYY13 pKa = 11.2VMQLEE18 pKa = 4.55GQKK21 pKa = 10.35LQAVFLAHH29 pKa = 6.8NNFVPNDD36 pKa = 3.5VSSYY40 pKa = 10.66DD41 pKa = 3.27HH42 pKa = 6.59MEE44 pKa = 4.16VVLSKK49 pKa = 10.55YY50 pKa = 9.82RR51 pKa = 11.84QSFDD55 pKa = 3.34YY56 pKa = 10.87RR57 pKa = 11.84EE58 pKa = 4.21SLSDD62 pKa = 4.12FYY64 pKa = 11.8SQGEE68 pKa = 4.12LPFRR72 pKa = 11.84WGSVMWCSRR81 pKa = 11.84VTGEE85 pKa = 4.53EE86 pKa = 4.15YY87 pKa = 10.83LSFMALMMDD96 pKa = 4.26LVKK99 pKa = 10.17IDD101 pKa = 3.55SSEE104 pKa = 4.15LKK106 pKa = 10.93GNEE109 pKa = 3.91HH110 pKa = 6.34PNIRR114 pKa = 11.84EE115 pKa = 3.96ALSWPTGSPTMGFIKK130 pKa = 10.32LNCLGSTWSLNYY142 pKa = 9.83QKK144 pKa = 11.06SRR146 pKa = 11.84VATLLLRR153 pKa = 11.84AGGSKK158 pKa = 10.55DD159 pKa = 3.31GLEE162 pKa = 3.83EE163 pKa = 5.6AIVKK167 pKa = 6.08THH169 pKa = 6.14KK170 pKa = 10.26KK171 pKa = 9.8ILMEE175 pKa = 4.02SSIRR179 pKa = 11.84GFDD182 pKa = 3.48PKK184 pKa = 10.61HH185 pKa = 6.05FPGLDD190 pKa = 4.1LIRR193 pKa = 11.84EE194 pKa = 4.38VACLQCVRR202 pKa = 11.84LMNASCFDD210 pKa = 4.14SVHH213 pKa = 5.56TAYY216 pKa = 9.9PSRR219 pKa = 11.84LLDD222 pKa = 3.5VLSMHH227 pKa = 7.09RR228 pKa = 11.84STYY231 pKa = 10.04TMISSKK237 pKa = 10.93LLGNRR242 pKa = 11.84KK243 pKa = 5.5WTPVQDD249 pKa = 3.55THH251 pKa = 8.57FDD253 pKa = 3.88EE254 pKa = 6.07PEE256 pKa = 3.54ATYY259 pKa = 11.04DD260 pKa = 3.65FSSDD264 pKa = 3.39SEE266 pKa = 4.28

Molecular weight: 30.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499S1A8|A0A499S1A8_9VIRU Glycoprotein OS=Ntepes virus OX=2569589 GN=GPC PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 3.29YY4 pKa = 11.22AQIAIEE10 pKa = 4.47FGSSGIDD17 pKa = 3.09RR18 pKa = 11.84DD19 pKa = 5.08LIEE22 pKa = 3.85AWVRR26 pKa = 11.84EE27 pKa = 3.95FEE29 pKa = 4.4YY30 pKa = 10.73QGFDD34 pKa = 3.13PKK36 pKa = 11.2VVVKK40 pKa = 10.49IVTEE44 pKa = 4.23TGEE47 pKa = 4.03GWQNDD52 pKa = 3.92VKK54 pKa = 11.4KK55 pKa = 10.31MIILALTRR63 pKa = 11.84GNKK66 pKa = 7.57PEE68 pKa = 3.97KK69 pKa = 9.99MIAKK73 pKa = 8.73MSAKK77 pKa = 9.62GRR79 pKa = 11.84EE80 pKa = 3.96EE81 pKa = 3.68VTRR84 pKa = 11.84LVKK87 pKa = 10.21KK88 pKa = 10.84YY89 pKa = 10.07KK90 pKa = 10.37LKK92 pKa = 10.78SGNPGRR98 pKa = 11.84NDD100 pKa = 3.09LTLSRR105 pKa = 11.84VAAAFASWTCAAIYY119 pKa = 9.27YY120 pKa = 8.47VQDD123 pKa = 3.73YY124 pKa = 11.05LPVTGSHH131 pKa = 6.29MDD133 pKa = 4.16AISRR137 pKa = 11.84NYY139 pKa = 8.3PRR141 pKa = 11.84AMMHH145 pKa = 6.7PSFAGLIDD153 pKa = 3.67PGMRR157 pKa = 11.84DD158 pKa = 3.18RR159 pKa = 11.84EE160 pKa = 4.29ILVEE164 pKa = 3.96AHH166 pKa = 6.84SLFLIEE172 pKa = 5.02FAQKK176 pKa = 10.53INVNLRR182 pKa = 11.84GKK184 pKa = 10.14SKK186 pKa = 11.46AEE188 pKa = 3.55ILLSFDD194 pKa = 3.42QPLNAAINSNFLTSSQKK211 pKa = 10.78LKK213 pKa = 10.99ALAALGVIDD222 pKa = 4.61EE223 pKa = 4.42HH224 pKa = 6.63QKK226 pKa = 9.98PNRR229 pKa = 11.84DD230 pKa = 3.26VVSAADD236 pKa = 3.89AFRR239 pKa = 11.84KK240 pKa = 8.23MM241 pKa = 3.96

Molecular weight: 26.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4038

241

2102

1009.5

113.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.894 ± 0.722

2.526 ± 0.668

5.225 ± 0.349

7.231 ± 0.364

4.383 ± 0.714

6.142 ± 0.175

2.377 ± 0.19

6.662 ± 0.608

6.612 ± 0.502

8.692 ± 0.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.12 ± 0.546

3.962 ± 0.288

3.888 ± 0.222

2.774 ± 0.304

5.721 ± 0.213

9.039 ± 0.369

5.869 ± 0.674

6.142 ± 0.153

1.189 ± 0.161

2.551 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski