Cucumber necrosis virus (CNV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus

Average proteome isoelectric point is 8.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P15185|MVP_CNV Movement protein OS=Cucumber necrosis virus OX=12143 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.06RR3 pKa = 11.84AIQRR7 pKa = 11.84SDD9 pKa = 3.0ARR11 pKa = 11.84EE12 pKa = 3.73QANSEE17 pKa = 4.07RR18 pKa = 11.84WDD20 pKa = 3.7GRR22 pKa = 11.84CGGTITPFKK31 pKa = 10.79LPDD34 pKa = 3.67EE35 pKa = 4.76SPSLLEE41 pKa = 3.9WRR43 pKa = 11.84LHH45 pKa = 5.33NSEE48 pKa = 4.05EE49 pKa = 5.04SEE51 pKa = 5.1DD52 pKa = 3.73KK53 pKa = 11.04DD54 pKa = 3.5HH55 pKa = 7.42PLGFKK60 pKa = 10.23EE61 pKa = 4.04SWSFGKK67 pKa = 10.55VVFKK71 pKa = 10.76RR72 pKa = 11.84YY73 pKa = 9.63LRR75 pKa = 11.84YY76 pKa = 10.52DD77 pKa = 3.29GTEE80 pKa = 3.89TSLHH84 pKa = 5.47RR85 pKa = 11.84TLGSWEE91 pKa = 4.02RR92 pKa = 11.84NSVNDD97 pKa = 3.41AASRR101 pKa = 11.84FLGVSQIGCTYY112 pKa = 10.29SIRR115 pKa = 11.84FRR117 pKa = 11.84GSCLTLSGGSRR128 pKa = 11.84TLQRR132 pKa = 11.84LIEE135 pKa = 3.89MAIRR139 pKa = 11.84TKK141 pKa = 10.3RR142 pKa = 11.84TMLQLTPCEE151 pKa = 4.17VEE153 pKa = 4.31GNVSRR158 pKa = 11.84RR159 pKa = 11.84RR160 pKa = 11.84PQGSEE165 pKa = 3.35AFEE168 pKa = 4.32NKK170 pKa = 9.68EE171 pKa = 4.03SEE173 pKa = 4.2

Molecular weight: 19.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q66164|Q66164_CNV RNA-directed RNA polymerase OS=Cucumber necrosis virus OX=12143 PE=4 SV=1
MM1 pKa = 7.5DD2 pKa = 5.19TIKK5 pKa = 10.95RR6 pKa = 11.84MLWPKK11 pKa = 10.64KK12 pKa = 9.66EE13 pKa = 3.93IFVGTFATGVEE24 pKa = 4.43RR25 pKa = 11.84DD26 pKa = 3.42TSVDD30 pKa = 2.92IFQLVCRR37 pKa = 11.84VVLRR41 pKa = 11.84YY42 pKa = 8.72MRR44 pKa = 11.84TGKK47 pKa = 9.8IEE49 pKa = 4.19NNTDD53 pKa = 3.04SLGNFIVEE61 pKa = 4.63LLKK64 pKa = 10.18TDD66 pKa = 5.21CAAKK70 pKa = 10.03WEE72 pKa = 4.24WFMKK76 pKa = 9.58RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84VGDD82 pKa = 3.54YY83 pKa = 10.84AKK85 pKa = 10.53SLAIASIPVIPLLSYY100 pKa = 10.2ATMKK104 pKa = 9.63KK105 pKa = 8.2TVALRR110 pKa = 11.84AFGNEE115 pKa = 3.24LSFNIRR121 pKa = 11.84VPRR124 pKa = 11.84PSVPKK129 pKa = 10.39KK130 pKa = 10.83GLLLRR135 pKa = 11.84LAAGLALAPICALAMYY151 pKa = 7.64ATLPRR156 pKa = 11.84EE157 pKa = 3.87KK158 pKa = 10.77LSVFKK163 pKa = 11.04LRR165 pKa = 11.84TEE167 pKa = 4.26ARR169 pKa = 11.84AHH171 pKa = 5.9MEE173 pKa = 4.18DD174 pKa = 3.5EE175 pKa = 5.34RR176 pKa = 11.84EE177 pKa = 4.2ATDD180 pKa = 3.71CLVVEE185 pKa = 5.21PARR188 pKa = 11.84EE189 pKa = 3.99LKK191 pKa = 10.99GKK193 pKa = 10.26DD194 pKa = 3.6GEE196 pKa = 4.5DD197 pKa = 3.55LLTGSRR203 pKa = 11.84MTKK206 pKa = 10.3VIASTGRR213 pKa = 11.84PRR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84PYY219 pKa = 9.67AAKK222 pKa = 9.43IAQVARR228 pKa = 11.84AKK230 pKa = 10.52VGYY233 pKa = 10.07LKK235 pKa = 9.25NTPEE239 pKa = 3.78NRR241 pKa = 11.84LIYY244 pKa = 9.39QRR246 pKa = 11.84VMIEE250 pKa = 5.34IMDD253 pKa = 4.32KK254 pKa = 11.03DD255 pKa = 3.68CVRR258 pKa = 11.84YY259 pKa = 9.97VDD261 pKa = 4.33RR262 pKa = 11.84DD263 pKa = 3.8VILPLAIGCCFVYY276 pKa = 10.38PDD278 pKa = 4.17GVEE281 pKa = 3.91EE282 pKa = 5.03SAALWGSDD290 pKa = 2.76EE291 pKa = 4.24SLGVKK296 pKa = 9.67

Molecular weight: 33.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1857

173

818

371.4

41.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.916 ± 0.836

1.723 ± 0.453

4.793 ± 0.302

6.031 ± 0.744

3.877 ± 0.315

7.324 ± 0.481

1.454 ± 0.409

4.685 ± 0.331

6.031 ± 0.637

9.639 ± 0.509

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.418

4.093 ± 0.873

4.847 ± 0.253

2.423 ± 0.33

7.647 ± 0.933

6.462 ± 1.121

5.924 ± 0.485

8.024 ± 0.575

1.562 ± 0.133

3.231 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski