Nocardia amikacinitolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales;

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285LV95|A0A285LV95_9NOCA 2 4-dienoyl-CoA reductase OS=Nocardia amikacinitolerans OX=756689 GN=SAMN04244553_5365 PE=3 SV=1
MM1 pKa = 6.63NTDD4 pKa = 3.3YY5 pKa = 11.71KK6 pKa = 10.98LFQCVQCGFEE16 pKa = 4.07YY17 pKa = 10.91DD18 pKa = 3.92EE19 pKa = 4.49ALGWPEE25 pKa = 5.14DD26 pKa = 4.18GIEE29 pKa = 4.69PGTRR33 pKa = 11.84WDD35 pKa = 5.76DD36 pKa = 4.2IPDD39 pKa = 3.72DD40 pKa = 4.45WSCPDD45 pKa = 3.97CGAAKK50 pKa = 10.43ADD52 pKa = 3.73FEE54 pKa = 4.42MVEE57 pKa = 4.14VSRR60 pKa = 11.84AA61 pKa = 3.2

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285LR78|A0A285LR78_9NOCA Uncharacterized protein OS=Nocardia amikacinitolerans OX=756689 GN=SAMN04244553_4380 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.72GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6460

0

6460

2146072

24

12184

332.2

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.575 ± 0.048

0.74 ± 0.011

6.182 ± 0.026

5.712 ± 0.026

2.956 ± 0.02

8.838 ± 0.028

2.168 ± 0.013

4.013 ± 0.018

1.981 ± 0.023

10.132 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.866 ± 0.012

2.0 ± 0.017

5.951 ± 0.029

2.752 ± 0.021

7.885 ± 0.038

5.128 ± 0.02

6.036 ± 0.024

8.541 ± 0.031

1.461 ± 0.013

2.081 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski