Amino acid dipepetide frequency for Mycobacterium phage Typha

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.253AlaAla: 15.253 ± 1.046
1.105AlaCys: 1.105 ± 0.24
8.413AlaAsp: 8.413 ± 0.566
6.756AlaGlu: 6.756 ± 0.678
1.912AlaPhe: 1.912 ± 0.233
9.135AlaGly: 9.135 ± 0.936
2.124AlaHis: 2.124 ± 0.342
5.269AlaIle: 5.269 ± 0.436
5.821AlaLys: 5.821 ± 0.652
7.988AlaLeu: 7.988 ± 0.636
3.654AlaMet: 3.654 ± 0.357
3.484AlaAsn: 3.484 ± 0.379
4.546AlaPro: 4.546 ± 0.555
4.546AlaGln: 4.546 ± 0.484
7.011AlaArg: 7.011 ± 0.741
4.036AlaSer: 4.036 ± 0.463
6.671AlaThr: 6.671 ± 0.532
7.775AlaVal: 7.775 ± 0.624
2.167AlaTrp: 2.167 ± 0.335
2.379AlaTyr: 2.379 ± 0.323
0.0AlaXaa: 0.0 ± 0.0
Cys
0.892CysAla: 0.892 ± 0.203
0.127CysCys: 0.127 ± 0.065
1.105CysAsp: 1.105 ± 0.261
0.382CysGlu: 0.382 ± 0.127
0.17CysPhe: 0.17 ± 0.081
1.742CysGly: 1.742 ± 0.363
0.34CysHis: 0.34 ± 0.138
0.382CysIle: 0.382 ± 0.153
0.212CysLys: 0.212 ± 0.097
0.85CysLeu: 0.85 ± 0.204
0.17CysMet: 0.17 ± 0.109
0.382CysAsn: 0.382 ± 0.125
1.487CysPro: 1.487 ± 0.329
0.467CysGln: 0.467 ± 0.145
0.977CysArg: 0.977 ± 0.232
0.255CysSer: 0.255 ± 0.109
0.552CysThr: 0.552 ± 0.155
0.595CysVal: 0.595 ± 0.163
0.467CysTrp: 0.467 ± 0.146
0.212CysTyr: 0.212 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
6.756AspAla: 6.756 ± 0.557
0.595AspCys: 0.595 ± 0.16
5.821AspAsp: 5.821 ± 0.709
5.354AspGlu: 5.354 ± 0.602
0.977AspPhe: 0.977 ± 0.23
6.713AspGly: 6.713 ± 0.511
1.445AspHis: 1.445 ± 0.332
2.167AspIle: 2.167 ± 0.306
1.997AspLys: 1.997 ± 0.232
5.778AspLeu: 5.778 ± 0.539
1.36AspMet: 1.36 ± 0.239
2.549AspAsn: 2.549 ± 0.37
5.396AspPro: 5.396 ± 0.535
3.654AspGln: 3.654 ± 0.434
4.844AspArg: 4.844 ± 0.518
2.039AspSer: 2.039 ± 0.382
4.461AspThr: 4.461 ± 0.411
3.909AspVal: 3.909 ± 0.427
1.445AspTrp: 1.445 ± 0.295
1.869AspTyr: 1.869 ± 0.34
0.0AspXaa: 0.0 ± 0.0
Glu
5.736GluAla: 5.736 ± 0.685
0.68GluCys: 0.68 ± 0.189
4.121GluAsp: 4.121 ± 0.504
3.314GluGlu: 3.314 ± 0.358
1.615GluPhe: 1.615 ± 0.236
4.376GluGly: 4.376 ± 0.435
1.19GluHis: 1.19 ± 0.181
1.572GluIle: 1.572 ± 0.252
1.402GluLys: 1.402 ± 0.246
5.991GluLeu: 5.991 ± 0.513
1.869GluMet: 1.869 ± 0.313
1.275GluAsn: 1.275 ± 0.267
2.974GluPro: 2.974 ± 0.502
2.464GluGln: 2.464 ± 0.316
4.079GluArg: 4.079 ± 0.425
2.932GluSer: 2.932 ± 0.341
3.357GluThr: 3.357 ± 0.435
6.076GluVal: 6.076 ± 0.908
1.402GluTrp: 1.402 ± 0.254
1.402GluTyr: 1.402 ± 0.242
0.0GluXaa: 0.0 ± 0.0
Phe
2.124PheAla: 2.124 ± 0.342
0.127PheCys: 0.127 ± 0.08
2.167PheAsp: 2.167 ± 0.326
1.275PheGlu: 1.275 ± 0.215
0.807PhePhe: 0.807 ± 0.221
2.549PheGly: 2.549 ± 0.433
0.467PheHis: 0.467 ± 0.164
0.722PheIle: 0.722 ± 0.168
0.892PheLys: 0.892 ± 0.179
1.657PheLeu: 1.657 ± 0.244
0.382PheMet: 0.382 ± 0.13
0.892PheAsn: 0.892 ± 0.205
1.402PhePro: 1.402 ± 0.263
1.147PheGln: 1.147 ± 0.263
1.785PheArg: 1.785 ± 0.272
1.02PheSer: 1.02 ± 0.192
1.827PheThr: 1.827 ± 0.252
1.785PheVal: 1.785 ± 0.209
0.425PheTrp: 0.425 ± 0.143
0.51PheTyr: 0.51 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
7.903GlyAla: 7.903 ± 0.955
1.275GlyCys: 1.275 ± 0.281
6.203GlyAsp: 6.203 ± 0.568
4.121GlyGlu: 4.121 ± 0.353
2.974GlyPhe: 2.974 ± 0.345
7.648GlyGly: 7.648 ± 1.34
2.337GlyHis: 2.337 ± 0.354
3.314GlyIle: 3.314 ± 0.356
2.209GlyLys: 2.209 ± 0.268
6.798GlyLeu: 6.798 ± 0.555
1.912GlyMet: 1.912 ± 0.28
3.484GlyAsn: 3.484 ± 0.455
3.909GlyPro: 3.909 ± 0.462
4.291GlyGln: 4.291 ± 0.384
5.523GlyArg: 5.523 ± 0.479
5.438GlySer: 5.438 ± 0.818
6.501GlyThr: 6.501 ± 0.678
6.458GlyVal: 6.458 ± 0.475
2.422GlyTrp: 2.422 ± 0.276
2.294GlyTyr: 2.294 ± 0.31
0.0GlyXaa: 0.0 ± 0.0
His
2.677HisAla: 2.677 ± 0.382
0.34HisCys: 0.34 ± 0.135
1.572HisAsp: 1.572 ± 0.287
0.85HisGlu: 0.85 ± 0.211
0.467HisPhe: 0.467 ± 0.14
2.379HisGly: 2.379 ± 0.343
1.105HisHis: 1.105 ± 0.265
0.722HisIle: 0.722 ± 0.177
1.02HisLys: 1.02 ± 0.209
2.209HisLeu: 2.209 ± 0.345
0.595HisMet: 0.595 ± 0.148
0.765HisAsn: 0.765 ± 0.181
1.785HisPro: 1.785 ± 0.276
1.275HisGln: 1.275 ± 0.257
1.742HisArg: 1.742 ± 0.305
0.807HisSer: 0.807 ± 0.169
1.572HisThr: 1.572 ± 0.329
1.954HisVal: 1.954 ± 0.327
0.467HisTrp: 0.467 ± 0.131
1.147HisTyr: 1.147 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
5.736IleAla: 5.736 ± 0.619
0.467IleCys: 0.467 ± 0.138
2.634IleAsp: 2.634 ± 0.27
2.549IleGlu: 2.549 ± 0.29
0.722IlePhe: 0.722 ± 0.251
3.484IleGly: 3.484 ± 0.491
0.85IleHis: 0.85 ± 0.194
1.19IleIle: 1.19 ± 0.259
1.275IleLys: 1.275 ± 0.25
1.785IleLeu: 1.785 ± 0.253
0.892IleMet: 0.892 ± 0.172
1.445IleAsn: 1.445 ± 0.196
2.507IlePro: 2.507 ± 0.388
1.232IleGln: 1.232 ± 0.235
2.762IleArg: 2.762 ± 0.366
1.785IleSer: 1.785 ± 0.288
2.974IleThr: 2.974 ± 0.351
2.337IleVal: 2.337 ± 0.292
0.68IleTrp: 0.68 ± 0.158
0.807IleTyr: 0.807 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
4.376LysAla: 4.376 ± 0.534
0.425LysCys: 0.425 ± 0.128
2.039LysAsp: 2.039 ± 0.319
1.445LysGlu: 1.445 ± 0.209
0.637LysPhe: 0.637 ± 0.148
2.804LysGly: 2.804 ± 0.423
0.807LysHis: 0.807 ± 0.167
1.19LysIle: 1.19 ± 0.181
1.402LysLys: 1.402 ± 0.382
3.229LysLeu: 3.229 ± 0.428
0.807LysMet: 0.807 ± 0.205
0.425LysAsn: 0.425 ± 0.132
2.082LysPro: 2.082 ± 0.345
1.062LysGln: 1.062 ± 0.234
2.889LysArg: 2.889 ± 0.426
1.232LysSer: 1.232 ± 0.255
2.039LysThr: 2.039 ± 0.338
2.677LysVal: 2.677 ± 0.282
0.85LysTrp: 0.85 ± 0.194
0.595LysTyr: 0.595 ± 0.146
0.0LysXaa: 0.0 ± 0.0
Leu
8.498LeuAla: 8.498 ± 0.54
0.935LeuCys: 0.935 ± 0.209
4.801LeuAsp: 4.801 ± 0.476
4.249LeuGlu: 4.249 ± 0.452
2.039LeuPhe: 2.039 ± 0.305
7.435LeuGly: 7.435 ± 0.558
1.742LeuHis: 1.742 ± 0.321
3.399LeuIle: 3.399 ± 0.34
2.762LeuLys: 2.762 ± 0.362
5.566LeuLeu: 5.566 ± 0.616
1.742LeuMet: 1.742 ± 0.277
1.785LeuAsn: 1.785 ± 0.381
4.589LeuPro: 4.589 ± 0.468
2.804LeuGln: 2.804 ± 0.363
4.504LeuArg: 4.504 ± 0.507
3.654LeuSer: 3.654 ± 0.426
7.52LeuThr: 7.52 ± 0.624
6.671LeuVal: 6.671 ± 0.654
1.53LeuTrp: 1.53 ± 0.276
1.36LeuTyr: 1.36 ± 0.208
0.0LeuXaa: 0.0 ± 0.0
Met
3.442MetAla: 3.442 ± 0.374
0.17MetCys: 0.17 ± 0.082
0.892MetAsp: 0.892 ± 0.228
0.935MetGlu: 0.935 ± 0.256
0.552MetPhe: 0.552 ± 0.178
1.657MetGly: 1.657 ± 0.264
0.51MetHis: 0.51 ± 0.136
1.19MetIle: 1.19 ± 0.24
0.51MetLys: 0.51 ± 0.126
2.252MetLeu: 2.252 ± 0.303
0.68MetMet: 0.68 ± 0.168
0.765MetAsn: 0.765 ± 0.178
1.275MetPro: 1.275 ± 0.193
0.382MetGln: 0.382 ± 0.144
1.742MetArg: 1.742 ± 0.279
1.954MetSer: 1.954 ± 0.296
3.994MetThr: 3.994 ± 0.396
1.572MetVal: 1.572 ± 0.302
0.51MetTrp: 0.51 ± 0.148
0.425MetTyr: 0.425 ± 0.115
0.0MetXaa: 0.0 ± 0.0
Asn
3.739AsnAla: 3.739 ± 0.555
0.595AsnCys: 0.595 ± 0.16
1.53AsnAsp: 1.53 ± 0.26
1.275AsnGlu: 1.275 ± 0.222
0.51AsnPhe: 0.51 ± 0.151
3.569AsnGly: 3.569 ± 0.424
1.062AsnHis: 1.062 ± 0.236
0.765AsnIle: 0.765 ± 0.19
0.892AsnLys: 0.892 ± 0.231
2.082AsnLeu: 2.082 ± 0.296
0.467AsnMet: 0.467 ± 0.12
0.552AsnAsn: 0.552 ± 0.159
2.762AsnPro: 2.762 ± 0.317
0.892AsnGln: 0.892 ± 0.223
2.507AsnArg: 2.507 ± 0.334
1.062AsnSer: 1.062 ± 0.213
1.912AsnThr: 1.912 ± 0.28
2.337AsnVal: 2.337 ± 0.329
0.34AsnTrp: 0.34 ± 0.134
0.807AsnTyr: 0.807 ± 0.175
0.0AsnXaa: 0.0 ± 0.0
Pro
6.543ProAla: 6.543 ± 0.591
0.552ProCys: 0.552 ± 0.155
5.184ProAsp: 5.184 ± 0.446
3.909ProGlu: 3.909 ± 0.503
1.232ProPhe: 1.232 ± 0.249
5.056ProGly: 5.056 ± 0.436
1.785ProHis: 1.785 ± 0.315
2.252ProIle: 2.252 ± 0.354
1.827ProLys: 1.827 ± 0.295
3.781ProLeu: 3.781 ± 0.381
1.317ProMet: 1.317 ± 0.258
1.827ProAsn: 1.827 ± 0.321
3.951ProPro: 3.951 ± 0.548
2.082ProGln: 2.082 ± 0.383
3.187ProArg: 3.187 ± 0.378
3.229ProSer: 3.229 ± 0.392
4.079ProThr: 4.079 ± 0.441
5.523ProVal: 5.523 ± 0.438
1.105ProTrp: 1.105 ± 0.242
1.19ProTyr: 1.19 ± 0.231
0.0ProXaa: 0.0 ± 0.0
Gln
3.781GlnAla: 3.781 ± 0.399
0.467GlnCys: 0.467 ± 0.178
1.997GlnAsp: 1.997 ± 0.291
1.827GlnGlu: 1.827 ± 0.254
0.935GlnPhe: 0.935 ± 0.237
2.847GlnGly: 2.847 ± 0.421
1.317GlnHis: 1.317 ± 0.263
2.124GlnIle: 2.124 ± 0.244
1.36GlnLys: 1.36 ± 0.305
3.951GlnLeu: 3.951 ± 0.412
1.572GlnMet: 1.572 ± 0.257
0.807GlnAsn: 0.807 ± 0.21
1.53GlnPro: 1.53 ± 0.248
1.53GlnGln: 1.53 ± 0.267
3.611GlnArg: 3.611 ± 0.365
1.827GlnSer: 1.827 ± 0.279
1.827GlnThr: 1.827 ± 0.324
3.314GlnVal: 3.314 ± 0.406
1.062GlnTrp: 1.062 ± 0.248
0.935GlnTyr: 0.935 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
7.011ArgAla: 7.011 ± 0.512
1.105ArgCys: 1.105 ± 0.284
4.291ArgAsp: 4.291 ± 0.457
4.036ArgGlu: 4.036 ± 0.409
1.954ArgPhe: 1.954 ± 0.298
4.759ArgGly: 4.759 ± 0.474
1.997ArgHis: 1.997 ± 0.292
2.464ArgIle: 2.464 ± 0.362
2.634ArgLys: 2.634 ± 0.431
4.844ArgLeu: 4.844 ± 0.485
2.167ArgMet: 2.167 ± 0.328
1.954ArgAsn: 1.954 ± 0.327
3.781ArgPro: 3.781 ± 0.513
2.507ArgGln: 2.507 ± 0.334
5.991ArgArg: 5.991 ± 0.61
3.866ArgSer: 3.866 ± 0.45
3.017ArgThr: 3.017 ± 0.359
5.948ArgVal: 5.948 ± 0.543
1.742ArgTrp: 1.742 ± 0.331
2.847ArgTyr: 2.847 ± 0.401
0.0ArgXaa: 0.0 ± 0.0
Ser
5.141SerAla: 5.141 ± 0.483
0.382SerCys: 0.382 ± 0.158
3.187SerAsp: 3.187 ± 0.303
2.252SerGlu: 2.252 ± 0.281
1.36SerPhe: 1.36 ± 0.222
5.141SerGly: 5.141 ± 0.723
1.232SerHis: 1.232 ± 0.285
1.7SerIle: 1.7 ± 0.276
1.317SerLys: 1.317 ± 0.318
3.866SerLeu: 3.866 ± 0.399
1.232SerMet: 1.232 ± 0.254
1.615SerAsn: 1.615 ± 0.231
2.252SerPro: 2.252 ± 0.267
1.827SerGln: 1.827 ± 0.292
2.889SerArg: 2.889 ± 0.318
1.445SerSer: 1.445 ± 0.297
2.337SerThr: 2.337 ± 0.282
4.036SerVal: 4.036 ± 0.408
1.275SerTrp: 1.275 ± 0.254
1.445SerTyr: 1.445 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
7.265ThrAla: 7.265 ± 0.621
0.765ThrCys: 0.765 ± 0.211
5.523ThrAsp: 5.523 ± 0.424
4.844ThrGlu: 4.844 ± 0.493
1.572ThrPhe: 1.572 ± 0.212
5.948ThrGly: 5.948 ± 0.597
1.487ThrHis: 1.487 ± 0.256
2.592ThrIle: 2.592 ± 0.482
2.039ThrLys: 2.039 ± 0.31
5.226ThrLeu: 5.226 ± 0.531
1.615ThrMet: 1.615 ± 0.265
1.954ThrAsn: 1.954 ± 0.233
5.481ThrPro: 5.481 ± 0.564
2.379ThrGln: 2.379 ± 0.329
3.951ThrArg: 3.951 ± 0.414
2.847ThrSer: 2.847 ± 0.406
5.906ThrThr: 5.906 ± 0.696
6.118ThrVal: 6.118 ± 0.553
1.445ThrTrp: 1.445 ± 0.288
1.572ThrTyr: 1.572 ± 0.272
0.0ThrXaa: 0.0 ± 0.0
Val
9.007ValAla: 9.007 ± 0.865
0.977ValCys: 0.977 ± 0.216
4.504ValAsp: 4.504 ± 0.484
6.331ValGlu: 6.331 ± 0.83
2.209ValPhe: 2.209 ± 0.381
5.141ValGly: 5.141 ± 0.562
2.039ValHis: 2.039 ± 0.267
3.527ValIle: 3.527 ± 0.405
2.549ValLys: 2.549 ± 0.367
5.523ValLeu: 5.523 ± 0.421
1.869ValMet: 1.869 ± 0.323
2.592ValAsn: 2.592 ± 0.36
5.396ValPro: 5.396 ± 0.535
2.124ValGln: 2.124 ± 0.309
4.376ValArg: 4.376 ± 0.474
4.121ValSer: 4.121 ± 0.452
6.586ValThr: 6.586 ± 0.665
7.011ValVal: 7.011 ± 0.634
1.869ValTrp: 1.869 ± 0.243
2.507ValTyr: 2.507 ± 0.349
0.0ValXaa: 0.0 ± 0.0
Trp
1.572TrpAla: 1.572 ± 0.275
0.297TrpCys: 0.297 ± 0.106
1.615TrpAsp: 1.615 ± 0.241
1.062TrpGlu: 1.062 ± 0.18
1.062TrpPhe: 1.062 ± 0.217
1.827TrpGly: 1.827 ± 0.241
1.062TrpHis: 1.062 ± 0.202
0.722TrpIle: 0.722 ± 0.163
0.467TrpLys: 0.467 ± 0.134
2.252TrpLeu: 2.252 ± 0.318
0.637TrpMet: 0.637 ± 0.17
0.637TrpAsn: 0.637 ± 0.19
1.062TrpPro: 1.062 ± 0.205
0.765TrpGln: 0.765 ± 0.242
1.997TrpArg: 1.997 ± 0.297
0.892TrpSer: 0.892 ± 0.192
1.36TrpThr: 1.36 ± 0.248
2.379TrpVal: 2.379 ± 0.345
0.382TrpTrp: 0.382 ± 0.118
0.212TrpTyr: 0.212 ± 0.098
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.634TyrAla: 2.634 ± 0.28
0.382TyrCys: 0.382 ± 0.13
1.53TyrAsp: 1.53 ± 0.25
1.062TyrGlu: 1.062 ± 0.221
0.255TyrPhe: 0.255 ± 0.135
2.804TyrGly: 2.804 ± 0.331
0.552TyrHis: 0.552 ± 0.159
0.85TyrIle: 0.85 ± 0.245
0.425TyrLys: 0.425 ± 0.134
1.954TyrLeu: 1.954 ± 0.291
0.425TyrMet: 0.425 ± 0.139
0.467TyrAsn: 0.467 ± 0.123
1.445TyrPro: 1.445 ± 0.272
1.19TyrGln: 1.19 ± 0.198
2.549TyrArg: 2.549 ± 0.362
1.487TyrSer: 1.487 ± 0.229
2.039TyrThr: 2.039 ± 0.255
1.785TyrVal: 1.785 ± 0.282
0.68TyrTrp: 0.68 ± 0.178
0.382TyrTyr: 0.382 ± 0.115
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 127 proteins (23537 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski