Hubei permutotetra-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHW9|A0A1L3KHW9_9VIRU RdRp OS=Hubei permutotetra-like virus 7 OX=1923081 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 5.69ASNPIIPNTLTTMSNILDD20 pKa = 3.53RR21 pKa = 11.84QARR24 pKa = 11.84MTRR27 pKa = 11.84QDD29 pKa = 3.35FSLLRR34 pKa = 11.84DD35 pKa = 3.58RR36 pKa = 11.84LSTQARR42 pKa = 11.84HH43 pKa = 5.57LLEE46 pKa = 4.75VDD48 pKa = 3.83VPRR51 pKa = 11.84LPPEE55 pKa = 3.83QVQEE59 pKa = 3.73ILKK62 pKa = 9.44EE63 pKa = 4.09YY64 pKa = 10.63KK65 pKa = 7.67EE66 pKa = 4.37TRR68 pKa = 11.84VAVPPDD74 pKa = 3.37SKK76 pKa = 11.59APLEE80 pKa = 4.36FPTLDD85 pKa = 4.09LYY87 pKa = 11.47AEE89 pKa = 5.29EE90 pKa = 4.61IPISRR95 pKa = 11.84KK96 pKa = 9.75LDD98 pKa = 3.38FDD100 pKa = 3.6GRR102 pKa = 11.84PIHH105 pKa = 6.66PAGTNSKK112 pKa = 9.75QGRR115 pKa = 11.84VHH117 pKa = 6.85LSKK120 pKa = 11.08SFGSQPRR127 pKa = 11.84PMEE130 pKa = 4.3GVSYY134 pKa = 10.25LAEE137 pKa = 3.77ILGRR141 pKa = 11.84DD142 pKa = 3.7VCNTTHH148 pKa = 7.44IYY150 pKa = 10.29TSGTRR155 pKa = 11.84AGFQKK160 pKa = 10.25RR161 pKa = 11.84VAKK164 pKa = 10.1QLGRR168 pKa = 11.84KK169 pKa = 8.76AVSLAKK175 pKa = 9.69MFRR178 pKa = 11.84EE179 pKa = 4.07VRR181 pKa = 11.84GLGKK185 pKa = 10.23NPTAKK190 pKa = 10.33YY191 pKa = 9.93VNAYY195 pKa = 10.33LDD197 pKa = 3.43TLMPRR202 pKa = 11.84KK203 pKa = 9.67PNTNWPGVHH212 pKa = 6.75TDD214 pKa = 4.63LIDD217 pKa = 5.14GIMDD221 pKa = 5.3GIKK224 pKa = 10.29INFKK228 pKa = 10.55SSAGAPYY235 pKa = 9.91HH236 pKa = 7.12RR237 pKa = 11.84PLHH240 pKa = 6.03LCADD244 pKa = 4.16MVLSTGLPAAIEE256 pKa = 4.24IIKK259 pKa = 10.23NSKK262 pKa = 9.14PIHH265 pKa = 6.73DD266 pKa = 4.1AMEE269 pKa = 5.36DD270 pKa = 3.48DD271 pKa = 4.1VEE273 pKa = 4.46FFISEE278 pKa = 4.58LKK280 pKa = 10.99NKK282 pKa = 7.79TDD284 pKa = 3.35RR285 pKa = 11.84YY286 pKa = 10.56DD287 pKa = 3.42VNEE290 pKa = 4.51LMDD293 pKa = 3.4KK294 pKa = 9.52TRR296 pKa = 11.84PYY298 pKa = 10.81CCLPAHH304 pKa = 6.3WRR306 pKa = 11.84LLFSLVSQGFSATHH320 pKa = 5.26PTFDD324 pKa = 4.51EE325 pKa = 4.25IKK327 pKa = 9.43SANAYY332 pKa = 8.77GHH334 pKa = 6.41SNAHH338 pKa = 5.14GGMRR342 pKa = 11.84RR343 pKa = 11.84RR344 pKa = 11.84YY345 pKa = 8.92DD346 pKa = 2.87WAMTANTRR354 pKa = 11.84GQVIAYY360 pKa = 9.67GDD362 pKa = 3.78DD363 pKa = 3.2ALISIKK369 pKa = 10.35RR370 pKa = 11.84GSDD373 pKa = 2.9IFLVAPDD380 pKa = 4.13FRR382 pKa = 11.84QMDD385 pKa = 4.11GSIHH389 pKa = 6.85RR390 pKa = 11.84EE391 pKa = 3.7DD392 pKa = 2.89IKK394 pKa = 11.54YY395 pKa = 8.86IFRR398 pKa = 11.84WILSHH403 pKa = 7.52LIRR406 pKa = 11.84DD407 pKa = 3.89APGADD412 pKa = 3.33VNFWGPLFSLWEE424 pKa = 4.05FFAIDD429 pKa = 4.92PILMCEE435 pKa = 4.01GSKK438 pKa = 10.72AYY440 pKa = 8.69TKK442 pKa = 10.54KK443 pKa = 10.8NPDD446 pKa = 3.17AGMLSGVPGTTLINTVKK463 pKa = 10.7CATAWDD469 pKa = 4.92LYY471 pKa = 11.19LEE473 pKa = 4.69HH474 pKa = 7.19CANTGSDD481 pKa = 3.74ALDD484 pKa = 3.98PDD486 pKa = 3.52MARR489 pKa = 11.84RR490 pKa = 11.84FMAKK494 pKa = 9.75HH495 pKa = 5.24GLEE498 pKa = 4.2LKK500 pKa = 10.34PGTFSPQRR508 pKa = 11.84LPTPDD513 pKa = 3.66EE514 pKa = 4.33FQHH517 pKa = 6.6GLLLTTTKK525 pKa = 10.58FLGLQTIVFEE535 pKa = 4.46RR536 pKa = 11.84NEE538 pKa = 4.44GEE540 pKa = 5.32FILCPHH546 pKa = 7.5LPRR549 pKa = 11.84DD550 pKa = 3.74EE551 pKa = 5.01CVSQLMTPRR560 pKa = 11.84EE561 pKa = 4.33DD562 pKa = 3.3ATGPRR567 pKa = 11.84PSLTASNRR575 pKa = 11.84TLYY578 pKa = 10.85DD579 pKa = 3.14RR580 pKa = 11.84ARR582 pKa = 11.84GLMVTHH588 pKa = 7.42CFTQPDD594 pKa = 3.62VRR596 pKa = 11.84AALYY600 pKa = 10.63NLANQIDD607 pKa = 4.12PTAIALIPQVEE618 pKa = 4.49NGLPPDD624 pKa = 3.89HH625 pKa = 7.08VSLPEE630 pKa = 3.74FSFPDD635 pKa = 3.36SSGFPDD641 pKa = 5.11LSFCLSLYY649 pKa = 9.88STDD652 pKa = 3.48EE653 pKa = 4.12KK654 pKa = 10.6QPPMHH659 pKa = 6.29QFYY662 pKa = 10.65PNISNVHH669 pKa = 6.96AEE671 pKa = 4.02MEE673 pKa = 4.34RR674 pKa = 11.84LRR676 pKa = 11.84EE677 pKa = 3.68AAKK680 pKa = 10.53FKK682 pKa = 10.63FEE684 pKa = 3.87RR685 pKa = 11.84VAVEE689 pKa = 3.86RR690 pKa = 11.84GAIVRR695 pKa = 11.84APSPAPRR702 pKa = 11.84PIDD705 pKa = 4.17PIMEE709 pKa = 4.21VAIGTTGKK717 pKa = 8.42STAVNVPKK725 pKa = 10.74NPPKK729 pKa = 10.15GSKK732 pKa = 8.79TVVVGSDD739 pKa = 3.04ATPPKK744 pKa = 10.33RR745 pKa = 11.84LPTLFMLIRR754 pKa = 11.84KK755 pKa = 8.84IVTRR759 pKa = 11.84RR760 pKa = 11.84GGACTVADD768 pKa = 3.77AAAYY772 pKa = 9.97ASAEE776 pKa = 3.84PRR778 pKa = 11.84RR779 pKa = 11.84VYY781 pKa = 11.0DD782 pKa = 3.38EE783 pKa = 4.41ALNGGLYY790 pKa = 9.28LTGTDD795 pKa = 4.08DD796 pKa = 5.03GDD798 pKa = 4.1IISLNPIATPFPTAATKK815 pKa = 10.15QRR817 pKa = 11.84SDD819 pKa = 3.13EE820 pKa = 4.06SRR822 pKa = 11.84IRR824 pKa = 11.84SLPPRR829 pKa = 11.84ASMAAFNRR837 pKa = 11.84KK838 pKa = 8.5RR839 pKa = 11.84RR840 pKa = 11.84GGPPITFKK848 pKa = 10.8PQLVTINLRR857 pKa = 11.84EE858 pKa = 4.14LYY860 pKa = 10.24GVPLYY865 pKa = 10.49KK866 pKa = 10.92YY867 pKa = 10.63KK868 pKa = 10.68DD869 pKa = 3.85DD870 pKa = 5.94NDD872 pKa = 3.78MLDD875 pKa = 3.15QFTAAIRR882 pKa = 11.84KK883 pKa = 8.45HH884 pKa = 5.6FSHH887 pKa = 6.72LRR889 pKa = 11.84WRR891 pKa = 11.84PILNDD896 pKa = 3.34AKK898 pKa = 10.73HH899 pKa = 5.18LAEE902 pKa = 4.36RR903 pKa = 11.84LEE905 pKa = 3.95VSKK908 pKa = 11.31DD909 pKa = 3.13GLEE912 pKa = 4.37WIDD915 pKa = 4.74AAICEE920 pKa = 4.53CPTVKK925 pKa = 10.61LARR928 pKa = 11.84QSIAWAVYY936 pKa = 8.87LANGYY941 pKa = 9.65EE942 pKa = 4.35IEE944 pKa = 4.48TGPDD948 pKa = 3.13LANKK952 pKa = 7.46TNPDD956 pKa = 3.83PKK958 pKa = 9.92PYY960 pKa = 9.44PALWVPNPNPNPDD973 pKa = 3.99PNGPWEE979 pKa = 4.56DD980 pKa = 3.56WDD982 pKa = 4.15TGPDD986 pKa = 3.67LDD988 pKa = 4.33TQAGYY993 pKa = 10.27AAAEE997 pKa = 4.11PLAAPEE1003 pKa = 4.25IAPLGQPDD1011 pKa = 3.75IPLTSLPRR1019 pKa = 11.84DD1020 pKa = 3.42LQEE1023 pKa = 4.95LSEE1026 pKa = 5.04HH1027 pKa = 6.18YY1028 pKa = 10.69NILQIKK1034 pKa = 9.13LAYY1037 pKa = 10.01LALCNLGVVDD1047 pKa = 4.43PATVILEE1054 pKa = 4.14AVGSSLHH1061 pKa = 5.54PEE1063 pKa = 3.8RR1064 pKa = 11.84RR1065 pKa = 11.84RR1066 pKa = 11.84TARR1069 pKa = 11.84EE1070 pKa = 3.93RR1071 pKa = 11.84IPDD1074 pKa = 3.83TNSAGPAVPKK1084 pKa = 10.35RR1085 pKa = 11.84PPHH1088 pKa = 6.06QKK1090 pKa = 9.4PQSAKK1095 pKa = 9.73PPRR1098 pKa = 11.84KK1099 pKa = 9.02EE1100 pKa = 3.55KK1101 pKa = 10.66TNIEE1105 pKa = 3.99RR1106 pKa = 11.84PLTKK1110 pKa = 9.94NQKK1113 pKa = 8.71IRR1115 pKa = 11.84EE1116 pKa = 4.1RR1117 pKa = 11.84KK1118 pKa = 8.68KK1119 pKa = 10.35RR1120 pKa = 11.84RR1121 pKa = 11.84LAEE1124 pKa = 3.85LRR1126 pKa = 11.84QLEE1129 pKa = 4.32NGAPTKK1135 pKa = 10.83NKK1137 pKa = 10.43SEE1139 pKa = 4.24TSNSSGYY1146 pKa = 11.13ASGDD1150 pKa = 2.94GDD1152 pKa = 4.17GPGKK1156 pKa = 10.56EE1157 pKa = 3.68NVGRR1161 pKa = 11.84HH1162 pKa = 5.28RR1163 pKa = 11.84YY1164 pKa = 4.66WHH1166 pKa = 6.83KK1167 pKa = 9.97EE1168 pKa = 3.87APPAPPPVRR1177 pKa = 11.84NTLRR1181 pKa = 11.84RR1182 pKa = 11.84IWWDD1186 pKa = 3.07SALGDD1191 pKa = 3.84GGLDD1195 pKa = 3.18EE1196 pKa = 4.76TQASRR1201 pKa = 11.84DD1202 pKa = 3.48ADD1204 pKa = 3.6LSHH1207 pKa = 6.71YY1208 pKa = 10.04LQEE1211 pKa = 4.22

Molecular weight: 134.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHW9|A0A1L3KHW9_9VIRU RdRp OS=Hubei permutotetra-like virus 7 OX=1923081 PE=4 SV=1
MM1 pKa = 6.7VRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84TRR8 pKa = 11.84AKK10 pKa = 9.62PATPAAMQAATVMALAKK27 pKa = 10.31KK28 pKa = 8.96MSAVTVTGTKK38 pKa = 8.98RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84PRR46 pKa = 11.84SGIPSGVSGGIQRR59 pKa = 11.84SAMEE63 pKa = 3.97GSTRR67 pKa = 11.84LKK69 pKa = 9.22RR70 pKa = 11.84HH71 pKa = 5.58EE72 pKa = 4.54MLTSLTISKK81 pKa = 10.67NSTHH85 pKa = 6.02LVKK88 pKa = 10.16TIAIQPSSFSLLKK101 pKa = 10.92GIAKK105 pKa = 10.12SFEE108 pKa = 3.86RR109 pKa = 11.84TVWHH113 pKa = 6.64SLTIFYY119 pKa = 10.23KK120 pKa = 10.39PAVATTFGGLIAYY133 pKa = 8.97GIDD136 pKa = 3.03WSSKK140 pKa = 9.32VGTDD144 pKa = 3.09ADD146 pKa = 4.18RR147 pKa = 11.84TKK149 pKa = 10.74VCSLTPVLTHH159 pKa = 6.65AAWMDD164 pKa = 3.7SEE166 pKa = 4.35RR167 pKa = 11.84APLRR171 pKa = 11.84VPKK174 pKa = 10.44KK175 pKa = 9.6LLQSRR180 pKa = 11.84RR181 pKa = 11.84FYY183 pKa = 11.05INGADD188 pKa = 4.47ADD190 pKa = 4.18DD191 pKa = 4.98AGPGNIVVSIDD202 pKa = 3.14ITASSADD209 pKa = 3.63VVVGEE214 pKa = 4.85LWCQYY219 pKa = 9.98DD220 pKa = 3.6VEE222 pKa = 4.6MTGTVVPP229 pKa = 4.81

Molecular weight: 24.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1440

229

1211

720.0

79.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.167 ± 0.644

1.181 ± 0.151

6.25 ± 0.923

4.931 ± 1.133

3.056 ± 0.428

6.25 ± 0.361

2.431 ± 0.335

5.347 ± 0.162

5.417 ± 0.342

8.819 ± 1.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.589

3.819 ± 1.23

8.472 ± 1.798

2.778 ± 0.291

7.569 ± 0.785

6.389 ± 1.577

6.667 ± 1.441

5.139 ± 1.762

1.389 ± 0.175

2.639 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski