Proteome-pI 2.0 - Proteome Isoelectric Point Database

Sida yellow leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|B3GNA4|B3GNA4_9GEMI Replication-associated protein OS=Sida yellow leaf curl virus OX=536083 GN=Rep PE=3 SV=1

MM1 pKa = 7.5LNSSSSTPPCIKK13 pKa = 9.2PQHH16 pKa = 6.04RR17 pKa = 11.84AAKK20 pKa = 9.98KK21 pKa = 8.51RR22 pKa = 11.84GTRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84IDD30 pKa = 3.78LEE32 pKa = 4.49CGCTIYY38 pKa = 11.32VHH40 pKa = 6.78ISCSNHH46 pKa = 5.2GFTHH50 pKa = 7.52RR51 pKa = 11.84GTHH54 pKa = 5.68HH55 pKa = 6.57CASSRR60 pKa = 11.84EE61 pKa = 3.83WRR63 pKa = 11.84LYY65 pKa = 10.99LGDD68 pKa = 3.57NKK70 pKa = 10.95SPVFQDD76 pKa = 3.07IPRR79 pKa = 11.84RR80 pKa = 11.84GLPVHH85 pKa = 7.43DD86 pKa = 5.09DD87 pKa = 3.33QSVPHH92 pKa = 7.41PDD94 pKa = 3.47PVQSQPQEE102 pKa = 4.61SIGSPQGISQLPSLDD117 pKa = 4.41DD118 pKa = 4.31FPDD121 pKa = 3.86SFWNDD126 pKa = 2.77LFDD129 pKa = 3.99

Molecular weight: 14.59 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B3GNA5|B3GNA5_9GEMI AC4 OS=Sida yellow leaf curl virus OX=536083 GN=AC4 PE=4 SV=1

MM1 pKa = 7.86KK2 pKa = 10.43LFRR5 pKa = 11.84CFNPFHH11 pKa = 6.05GQSLNPHH18 pKa = 5.94TYY20 pKa = 9.94EE21 pKa = 3.97SPEE24 pKa = 3.97RR25 pKa = 11.84STPTASLIFTVLSNSPASPTSRR47 pKa = 11.84MLDD50 pKa = 3.79FSTSLTPDD58 pKa = 3.97GLPDD62 pKa = 3.58FTPIFRR68 pKa = 11.84QPKK71 pKa = 8.11TPTPSRR77 pKa = 11.84ITSPKK82 pKa = 9.71RR83 pKa = 11.84VIIVNPDD90 pKa = 2.92STRR93 pKa = 11.84CLGEE97 pKa = 3.4QRR99 pKa = 11.84GIKK102 pKa = 6.53TTYY105 pKa = 9.88TITPSMQLLRR115 pKa = 11.84EE116 pKa = 4.02RR117 pKa = 11.84LLTLSKK123 pKa = 10.91PEE125 pKa = 4.03TPRR128 pKa = 11.84LSSS131 pKa = 3.21

Molecular weight: 14.68 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
5	0	5	999	129	356	199.8	22.86

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
4.505 ± 0.768	1.702 ± 0.37	5.205 ± 0.656	4.204 ± 0.54	4.404 ± 0.245	5.706 ± 0.669	3.504 ± 0.462	5.005 ± 0.486	6.106 ± 1.045	7.508 ± 1.004

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.902 ± 0.403	5.706 ± 0.658	7.107 ± 1.182	3.804 ± 0.402	6.807 ± 1.066	7.808 ± 1.125	6.607 ± 1.428	6.406 ± 0.916	1.602 ± 0.245	4.404 ± 0.742

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski