Pararhodospirillum oryzae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Pararhodospirillum

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512H4N5|A0A512H4N5_9PROT Uncharacterized protein OS=Pararhodospirillum oryzae OX=478448 GN=ROR02_04710 PE=3 SV=1
MM1 pKa = 6.61TTITSSSYY9 pKa = 7.72VTGSSGTTYY18 pKa = 11.16LSGLSDD24 pKa = 4.87LDD26 pKa = 3.55TSALIEE32 pKa = 4.19AAVEE36 pKa = 4.26AKK38 pKa = 8.25MQPAYY43 pKa = 10.74RR44 pKa = 11.84LDD46 pKa = 3.92DD47 pKa = 4.8KK48 pKa = 11.32ISALEE53 pKa = 4.15TEE55 pKa = 4.62EE56 pKa = 4.6SAFSEE61 pKa = 4.43MTSLLSAVSEE71 pKa = 4.05ALEE74 pKa = 4.19PLASASVLSSSSDD87 pKa = 3.33TTSAFAGRR95 pKa = 11.84SAYY98 pKa = 8.46LTSSLDD104 pKa = 3.33TPANYY109 pKa = 9.97VGLTVTDD116 pKa = 3.84EE117 pKa = 4.44ADD119 pKa = 3.24LGNYY123 pKa = 7.14TLSVEE128 pKa = 4.71QLATTQKK135 pKa = 10.29VASQGLSAGTALGVDD150 pKa = 4.33GSFTLQADD158 pKa = 3.61DD159 pKa = 4.0GTAVDD164 pKa = 3.72ITVDD168 pKa = 3.36ASMTASDD175 pKa = 3.09IAEE178 pKa = 4.96AINEE182 pKa = 3.91QSATSGVVATVVRR195 pKa = 11.84TGSNEE200 pKa = 3.62QTLVLSSVNTGQAFTLTEE218 pKa = 3.97TSGTAGQALGLVDD231 pKa = 4.23SSGDD235 pKa = 3.43FVKK238 pKa = 10.43PVQEE242 pKa = 4.03AQQAILTLDD251 pKa = 3.67GVTVTSDD258 pKa = 3.26SNDD261 pKa = 3.2IKK263 pKa = 11.26DD264 pKa = 4.19LLPGVSVNLYY274 pKa = 9.88AATGGEE280 pKa = 4.38TIAVEE285 pKa = 4.22VGQDD289 pKa = 3.23LGAALDD295 pKa = 4.54AIEE298 pKa = 5.08AFVEE302 pKa = 4.51AYY304 pKa = 9.44NAYY307 pKa = 10.32RR308 pKa = 11.84EE309 pKa = 4.21FALTQQATDD318 pKa = 3.39EE319 pKa = 4.55SGAADD324 pKa = 3.76DD325 pKa = 5.34AVLFGDD331 pKa = 3.98STLKK335 pKa = 10.69GVNAALYY342 pKa = 9.68SALNTSVTVDD352 pKa = 3.0GTTYY356 pKa = 10.09TLSDD360 pKa = 3.41MGLSFNDD367 pKa = 4.18DD368 pKa = 3.05NTLALDD374 pKa = 3.81GDD376 pKa = 4.65VIEE379 pKa = 5.91EE380 pKa = 4.14MLLQNPDD387 pKa = 2.92VVEE390 pKa = 4.84AFFCSSVSCDD400 pKa = 3.26SSSLGVVSLPATMPSGTYY418 pKa = 9.62TLDD421 pKa = 3.19IDD423 pKa = 3.82VDD425 pKa = 3.81EE426 pKa = 4.81ATGKK430 pKa = 9.91IASATLNGVALTVSGTTLSGPDD452 pKa = 3.3GSIYY456 pKa = 10.26EE457 pKa = 4.6DD458 pKa = 2.99LWMGYY463 pKa = 9.97SGTGDD468 pKa = 3.3ATITLAVSTGLADD481 pKa = 4.42ALTTAIDD488 pKa = 4.45DD489 pKa = 3.84YY490 pKa = 11.58TNAGDD495 pKa = 4.05GRR497 pKa = 11.84ITTRR501 pKa = 11.84LSSIADD507 pKa = 4.56DD508 pKa = 3.37ITGKK512 pKa = 8.03QEE514 pKa = 3.54KK515 pKa = 9.29RR516 pKa = 11.84DD517 pKa = 4.15RR518 pKa = 11.84IAEE521 pKa = 4.0RR522 pKa = 11.84AGDD525 pKa = 3.69YY526 pKa = 9.63EE527 pKa = 4.27EE528 pKa = 6.29RR529 pKa = 11.84MIAYY533 pKa = 7.77YY534 pKa = 10.82AKK536 pKa = 10.64LEE538 pKa = 4.21EE539 pKa = 4.57QISMADD545 pKa = 2.92IRR547 pKa = 11.84LQQIEE552 pKa = 4.24ALFYY556 pKa = 11.29GDD558 pKa = 5.55DD559 pKa = 3.79DD560 pKa = 4.03

Molecular weight: 57.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512H3N1|A0A512H3N1_9PROT Peptide chain release factor 1 OS=Pararhodospirillum oryzae OX=478448 GN=prfA PE=3 SV=1
MM1 pKa = 7.5SALSLISRR9 pKa = 11.84HH10 pKa = 6.12PVATWRR16 pKa = 11.84LWHH19 pKa = 6.74AGLVGSGLVAYY30 pKa = 7.49VTADD34 pKa = 3.21EE35 pKa = 4.4DD36 pKa = 4.47TYY38 pKa = 11.93ALHH41 pKa = 6.24QFSGYY46 pKa = 10.15LFAVLAVGRR55 pKa = 11.84LIVAAAPGIRR65 pKa = 11.84GPWRR69 pKa = 11.84LPQPGGKK76 pKa = 9.36AAAPARR82 pKa = 11.84LTRR85 pKa = 11.84GAMFQRR91 pKa = 11.84VRR93 pKa = 11.84PLMTAVLIGVLLAAGLSGVGADD115 pKa = 3.42WIHH118 pKa = 6.59RR119 pKa = 11.84LEE121 pKa = 4.42KK122 pKa = 10.28LHH124 pKa = 6.24EE125 pKa = 4.55AVGEE129 pKa = 3.99ATPWVVLAHH138 pKa = 5.41VAVVVALLARR148 pKa = 11.84LPQGLRR154 pKa = 11.84RR155 pKa = 11.84MATPQRR161 pKa = 11.84ALSALAAAGLLATLMGAAPPARR183 pKa = 11.84AADD186 pKa = 3.83PQQALLGVFTEE197 pKa = 4.48RR198 pKa = 11.84AKK200 pKa = 10.94AATPGFAGFDD210 pKa = 3.81PKK212 pKa = 10.63RR213 pKa = 11.84GEE215 pKa = 4.02ALFLSRR221 pKa = 11.84NTANPEE227 pKa = 4.02LPSCASCHH235 pKa = 5.34TQDD238 pKa = 3.27PRR240 pKa = 11.84ASGRR244 pKa = 11.84HH245 pKa = 5.11AKK247 pKa = 9.04TGRR250 pKa = 11.84AIDD253 pKa = 3.73PMAPSVNRR261 pKa = 11.84EE262 pKa = 3.76RR263 pKa = 11.84FTDD266 pKa = 4.18LAQVDD271 pKa = 3.74KK272 pKa = 11.14RR273 pKa = 11.84LGRR276 pKa = 11.84DD277 pKa = 3.27CEE279 pKa = 4.5TVLGRR284 pKa = 11.84ACTPLEE290 pKa = 3.97QGDD293 pKa = 4.3FVAFMLSRR301 pKa = 3.88

Molecular weight: 31.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3199

0

3199

1075380

44

2547

336.2

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.258 ± 0.068

0.905 ± 0.015

5.729 ± 0.032

5.634 ± 0.037

3.202 ± 0.026

8.848 ± 0.045

2.134 ± 0.019

4.03 ± 0.036

2.276 ± 0.026

11.241 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.019

2.083 ± 0.025

6.158 ± 0.046

2.952 ± 0.024

7.973 ± 0.04

4.853 ± 0.028

5.455 ± 0.031

7.924 ± 0.033

1.324 ± 0.016

1.813 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski