Novosphingobium tardaugens NBRC 16725

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Caenibius; Caenibius tardaugens

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4005 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2ZYM3|U2ZYM3_9SPHN Uncharacterized protein OS=Novosphingobium tardaugens NBRC 16725 OX=1219035 GN=NT2_01_03990 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 10.45YY3 pKa = 10.67LLLSAVAASAMLAASPAYY21 pKa = 10.06ANRR24 pKa = 11.84DD25 pKa = 3.38RR26 pKa = 11.84DD27 pKa = 3.74HH28 pKa = 7.2GSDD31 pKa = 3.01IDD33 pKa = 4.14VNYY36 pKa = 10.11EE37 pKa = 3.94NNIKK41 pKa = 9.73TSIVTDD47 pKa = 3.11VAYY50 pKa = 10.73YY51 pKa = 10.78KK52 pKa = 10.71DD53 pKa = 3.54VALEE57 pKa = 4.2GTVSLEE63 pKa = 3.76GTISVDD69 pKa = 3.12SSAVAVTDD77 pKa = 3.57VKK79 pKa = 10.96QLSGLNNVTYY89 pKa = 10.63RR90 pKa = 11.84EE91 pKa = 4.04EE92 pKa = 4.21NEE94 pKa = 4.06LNGEE98 pKa = 4.01NGYY101 pKa = 10.73VSDD104 pKa = 3.89IFGPGWSEE112 pKa = 4.74AGNDD116 pKa = 3.86PNDD119 pKa = 3.44NLTNGVLEE127 pKa = 4.02GAIRR131 pKa = 11.84VGYY134 pKa = 7.67FAPIINTVDD143 pKa = 3.12AFDD146 pKa = 3.53VSGAGNIGVNLSAGYY161 pKa = 11.38YY162 pKa = 8.64NMQMNSATLASSSVQDD178 pKa = 3.93PDD180 pKa = 4.89ASGGWSEE187 pKa = 5.4ASTTSLQMLVGTYY200 pKa = 9.87QYY202 pKa = 11.38GAEE205 pKa = 4.05TDD207 pKa = 3.88VLPEE211 pKa = 5.17DD212 pKa = 5.03DD213 pKa = 4.34PTDD216 pKa = 3.73GGGGNNFRR224 pKa = 11.84DD225 pKa = 4.07RR226 pKa = 11.84NTVMGGAVSGDD237 pKa = 3.33GNIGINAAAGSFNQQANLLTLAVATDD263 pKa = 3.45AALAEE268 pKa = 4.65ANSGLLQIAVFNGVEE283 pKa = 4.08QQDD286 pKa = 4.2SINTVGTLTIADD298 pKa = 3.45ASGNIGVNMAAGVGNQQLNSLTIATSLASGGGDD331 pKa = 3.32GNGGGGGDD339 pKa = 3.79GSS341 pKa = 3.85

Molecular weight: 34.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2Y5W4|U2Y5W4_9SPHN Putative multidrug resistance protein B OS=Novosphingobium tardaugens NBRC 16725 OX=1219035 GN=NT2_03_00140 PE=4 SV=1
MM1 pKa = 7.59LRR3 pKa = 11.84PSHH6 pKa = 6.45TILRR10 pKa = 11.84ATILRR15 pKa = 11.84PALCATLLAAGLASPAMARR34 pKa = 11.84DD35 pKa = 3.28SLGIYY40 pKa = 9.79DD41 pKa = 3.19SWGAFRR47 pKa = 11.84DD48 pKa = 3.8PGAQPRR54 pKa = 11.84CYY56 pKa = 10.3AIAKK60 pKa = 8.42ANPGTARR67 pKa = 11.84RR68 pKa = 11.84DD69 pKa = 3.86FEE71 pKa = 4.24PTASVGTWPKK81 pKa = 10.27RR82 pKa = 11.84QIRR85 pKa = 11.84NQIHH89 pKa = 5.78FRR91 pKa = 11.84LSRR94 pKa = 11.84KK95 pKa = 9.11ILPAGKK101 pKa = 8.26ITLFLGGKK109 pKa = 9.45RR110 pKa = 11.84FALTGGGADD119 pKa = 3.3AWAVDD124 pKa = 3.68AAMDD128 pKa = 4.08AAIVAAMRR136 pKa = 11.84SAGSMSVSALDD147 pKa = 3.47EE148 pKa = 4.52RR149 pKa = 11.84GNAFTNTYY157 pKa = 9.63GLAGAATALDD167 pKa = 3.94AALLGCAKK175 pKa = 10.3LRR177 pKa = 3.98

Molecular weight: 18.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4005

0

4005

1284881

41

4966

320.8

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.777 ± 0.056

0.851 ± 0.014

6.077 ± 0.029

5.391 ± 0.037

3.599 ± 0.027

8.947 ± 0.088

2.107 ± 0.02

5.157 ± 0.026

2.99 ± 0.028

9.75 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.022

2.788 ± 0.029

5.21 ± 0.032

3.345 ± 0.021

7.064 ± 0.047

5.269 ± 0.03

5.337 ± 0.036

7.058 ± 0.033

1.432 ± 0.016

2.36 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski