Thalassomonas actiniarum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Thalassomonas

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D8D3D5|A0A0D8D3D5_9GAMM Uncharacterized protein OS=Thalassomonas actiniarum OX=485447 GN=SG35_04430 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 9.96KK3 pKa = 10.28AAIALTLTTLLSASVQADD21 pKa = 3.71TLLGVYY27 pKa = 10.51FGGQVWDD34 pKa = 4.14NEE36 pKa = 4.31AEE38 pKa = 4.51GVFGEE43 pKa = 4.81STTQSDD49 pKa = 4.14FNLKK53 pKa = 10.43DD54 pKa = 3.5EE55 pKa = 4.39QQGSFFVAVEE65 pKa = 4.21HH66 pKa = 6.58PLPFLPNVKK75 pKa = 9.68LAHH78 pKa = 6.44TSLDD82 pKa = 3.41TDD84 pKa = 3.89GNTTLTTDD92 pKa = 4.97FEE94 pKa = 5.41FNDD97 pKa = 3.43QTFTANTSVDD107 pKa = 3.16AVFDD111 pKa = 3.58VSYY114 pKa = 11.56NDD116 pKa = 3.1YY117 pKa = 9.33TFYY120 pKa = 11.65YY121 pKa = 10.52EE122 pKa = 6.06LFDD125 pKa = 4.85NDD127 pKa = 5.06LLTFDD132 pKa = 5.03FGLTARR138 pKa = 11.84DD139 pKa = 3.03LDD141 pKa = 4.07GDD143 pKa = 4.15VKK145 pKa = 11.26VADD148 pKa = 3.84TGNTQSASRR157 pKa = 11.84SVSEE161 pKa = 4.33IVPMVYY167 pKa = 10.15SSAIIGLPFTDD178 pKa = 2.86WNLFAEE184 pKa = 4.85GNYY187 pKa = 8.95LTFDD191 pKa = 3.28DD192 pKa = 4.47HH193 pKa = 6.81TLYY196 pKa = 10.64DD197 pKa = 3.84YY198 pKa = 11.25QVGVSYY204 pKa = 11.46ALTEE208 pKa = 4.04NFALDD213 pKa = 3.59VNLTAGYY220 pKa = 9.64RR221 pKa = 11.84AVKK224 pKa = 10.55FEE226 pKa = 5.95LDD228 pKa = 4.12DD229 pKa = 5.79LDD231 pKa = 5.78DD232 pKa = 5.3FYY234 pKa = 12.12ADD236 pKa = 4.74LEE238 pKa = 4.58FKK240 pKa = 10.98GLFAGVIMHH249 pKa = 7.02FF250 pKa = 3.97

Molecular weight: 27.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D8D185|A0A0D8D185_9GAMM TetR family transcriptional regulator OS=Thalassomonas actiniarum OX=485447 GN=SG35_06110 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84RR3 pKa = 11.84QKK5 pKa = 10.54RR6 pKa = 11.84DD7 pKa = 2.99RR8 pKa = 11.84LGRR11 pKa = 11.84AFSNGYY17 pKa = 8.44QAGLLGRR24 pKa = 11.84NKK26 pKa = 9.98EE27 pKa = 3.92NCPYY31 pKa = 10.91QNTNAKK37 pKa = 10.06SEE39 pKa = 4.12WLGGWRR45 pKa = 11.84EE46 pKa = 3.85AMTDD50 pKa = 3.28RR51 pKa = 11.84NTGLFKK57 pKa = 10.98

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5460

0

5460

1788439

31

7334

327.6

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.751 ± 0.033

0.991 ± 0.013

5.655 ± 0.03

6.025 ± 0.032

4.232 ± 0.026

6.849 ± 0.041

2.168 ± 0.02

6.204 ± 0.028

5.428 ± 0.039

10.693 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.017

4.564 ± 0.035

3.81 ± 0.023

5.052 ± 0.036

4.276 ± 0.025

6.862 ± 0.036

5.281 ± 0.033

6.424 ± 0.03

1.176 ± 0.014

3.243 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski