Natronincola peptidivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Natronincola

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0E5P9|A0A1I0E5P9_9CLOT Uncharacterized protein OS=Natronincola peptidivorans OX=426128 GN=SAMN05660297_02286 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.27KK3 pKa = 10.01IISLIILVLLALTIFTGCSYY23 pKa = 9.12EE24 pKa = 4.21TEE26 pKa = 4.2KK27 pKa = 11.15SEE29 pKa = 4.25EE30 pKa = 3.95ALQEE34 pKa = 4.7GITEE38 pKa = 4.48EE39 pKa = 4.11IEE41 pKa = 4.03EE42 pKa = 4.97EE43 pKa = 4.04IPQEE47 pKa = 4.02QEE49 pKa = 3.24VDD51 pKa = 3.1IADD54 pKa = 3.93EE55 pKa = 4.32NQEE58 pKa = 4.23STSDD62 pKa = 3.47FATDD66 pKa = 3.51EE67 pKa = 3.91EE68 pKa = 4.84RR69 pKa = 11.84TFYY72 pKa = 11.02LSNLRR77 pKa = 11.84NGDD80 pKa = 3.61EE81 pKa = 4.18VGEE84 pKa = 4.06GLIIRR89 pKa = 11.84DD90 pKa = 3.38IYY92 pKa = 10.96IEE94 pKa = 4.33DD95 pKa = 4.44EE96 pKa = 4.19YY97 pKa = 11.3TSFILAGDD105 pKa = 3.62IALTGEE111 pKa = 4.47LSYY114 pKa = 11.44DD115 pKa = 3.36HH116 pKa = 6.87QYY118 pKa = 10.17YY119 pKa = 9.56EE120 pKa = 4.97AMMFTFPDD128 pKa = 4.72DD129 pKa = 3.39IFPTIVIEE137 pKa = 4.38GEE139 pKa = 4.56GYY141 pKa = 9.03NQEE144 pKa = 4.75HH145 pKa = 6.89IEE147 pKa = 4.09FTYY150 pKa = 10.49KK151 pKa = 10.19PSHH154 pKa = 5.16FVFRR158 pKa = 11.84NEE160 pKa = 3.88DD161 pKa = 3.47ALFEE165 pKa = 4.6ALPHH169 pKa = 6.41SDD171 pKa = 3.72LIEE174 pKa = 4.37VIEE177 pKa = 4.54EE178 pKa = 4.23GKK180 pKa = 9.0TMNVGIGVKK189 pKa = 9.99DD190 pKa = 3.89LNFIGYY196 pKa = 7.04WQSEE200 pKa = 4.33YY201 pKa = 10.6GASVEE206 pKa = 4.07FVEE209 pKa = 6.27IIDD212 pKa = 4.08IMM214 pKa = 4.29

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0GAZ5|A0A1I0GAZ5_9CLOT AhpC/TSA family protein OS=Natronincola peptidivorans OX=426128 GN=SAMN05660297_03124 PE=4 SV=1
MM1 pKa = 7.74ALHH4 pKa = 6.6KK5 pKa = 10.75NKK7 pKa = 9.71FMEE10 pKa = 5.32FIEE13 pKa = 4.81GRR15 pKa = 11.84LLLLIGISTTMIGLFSPWVRR35 pKa = 11.84IEE37 pKa = 3.95WKK39 pKa = 9.84HH40 pKa = 5.51SRR42 pKa = 11.84GILDD46 pKa = 3.53STGMIIFCILIYY58 pKa = 10.02LTMIKK63 pKa = 9.98RR64 pKa = 11.84IAGYY68 pKa = 10.02RR69 pKa = 11.84IIASSIAIAILMAVQFMNFPRR90 pKa = 11.84IMNITWEE97 pKa = 4.09RR98 pKa = 11.84SFGFSMTYY106 pKa = 9.41VQTGFFITLFGVLLMMLAGFIKK128 pKa = 9.96QRR130 pKa = 11.84QGIRR134 pKa = 3.19

Molecular weight: 15.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3528

0

3528

1074561

11

2835

304.6

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.577 ± 0.044

0.92 ± 0.014

5.154 ± 0.03

7.917 ± 0.044

4.086 ± 0.035

6.862 ± 0.047

1.816 ± 0.015

9.532 ± 0.049

7.774 ± 0.049

9.341 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.018

4.957 ± 0.032

3.245 ± 0.025

3.17 ± 0.028

3.832 ± 0.027

5.508 ± 0.028

5.258 ± 0.034

6.622 ± 0.039

0.761 ± 0.014

3.812 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski