Sewage-associated circular DNA virus-28

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI50|A0A0B4UI50_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-28 OX=1592095 PE=3 SV=1
MM1 pKa = 7.51EE2 pKa = 5.94APPPAYY8 pKa = 10.11SPPPPSVTPPIEE20 pKa = 3.79SSRR23 pKa = 11.84PFRR26 pKa = 11.84LAGKK30 pKa = 10.05AIFLTWPQNDD40 pKa = 3.12ITKK43 pKa = 9.98EE44 pKa = 3.72DD45 pKa = 3.74LMAKK49 pKa = 10.15LVSLWEE55 pKa = 4.59AKK57 pKa = 10.55LSWAVVAEE65 pKa = 4.39EE66 pKa = 4.18SHH68 pKa = 7.01KK69 pKa = 11.02SGEE72 pKa = 4.21PHH74 pKa = 5.03VHH76 pKa = 7.26AIAQFTEE83 pKa = 5.05RR84 pKa = 11.84IDD86 pKa = 3.89LKK88 pKa = 10.96NANPVLDD95 pKa = 4.42ALTGKK100 pKa = 9.52HH101 pKa = 6.22GNYY104 pKa = 9.84QSVKK108 pKa = 9.21SAKK111 pKa = 9.23KK112 pKa = 9.2VLRR115 pKa = 11.84YY116 pKa = 9.18VCKK119 pKa = 9.43DD120 pKa = 3.09GQYY123 pKa = 8.29ITHH126 pKa = 6.75GEE128 pKa = 4.15VPDD131 pKa = 4.09FAEE134 pKa = 4.7KK135 pKa = 10.82AKK137 pKa = 10.76LQDD140 pKa = 3.17WAAKK144 pKa = 10.44LIMEE148 pKa = 4.62EE149 pKa = 3.84QATYY153 pKa = 10.76RR154 pKa = 11.84DD155 pKa = 4.06LVRR158 pKa = 11.84QQPGYY163 pKa = 11.6CMLQKK168 pKa = 10.53PKK170 pKa = 10.8LEE172 pKa = 4.13EE173 pKa = 4.15FIGWSKK179 pKa = 10.46RR180 pKa = 11.84QRR182 pKa = 11.84LADD185 pKa = 3.77SLLPWRR191 pKa = 11.84VLTVKK196 pKa = 10.46PNAPYY201 pKa = 10.37VHH203 pKa = 6.02QALWTFLRR211 pKa = 11.84EE212 pKa = 3.99NVLVPRR218 pKa = 11.84TPRR221 pKa = 11.84QAQLWLSGLPGVGKK235 pKa = 8.43TRR237 pKa = 11.84FLSYY241 pKa = 10.42LRR243 pKa = 11.84ARR245 pKa = 11.84LRR247 pKa = 11.84VYY249 pKa = 11.14DD250 pKa = 3.82MPRR253 pKa = 11.84DD254 pKa = 3.33EE255 pKa = 6.07DD256 pKa = 4.6FYY258 pKa = 11.64DD259 pKa = 4.55DD260 pKa = 6.03FEE262 pKa = 6.19DD263 pKa = 4.19GCFDD267 pKa = 5.14LVVLDD272 pKa = 4.06EE273 pKa = 5.2FKK275 pKa = 10.97AHH277 pKa = 7.15KK278 pKa = 10.11KK279 pKa = 9.6IQFLNAWADD288 pKa = 3.87GQPLPLRR295 pKa = 11.84KK296 pKa = 9.62KK297 pKa = 10.06GSQSVKK303 pKa = 9.84TDD305 pKa = 3.59NLPLIIVSNYY315 pKa = 9.43TIEE318 pKa = 4.05EE319 pKa = 4.69CYY321 pKa = 10.41KK322 pKa = 10.85SGVGRR327 pKa = 11.84DD328 pKa = 3.46ALVDD332 pKa = 3.44RR333 pKa = 11.84FTQVFVDD340 pKa = 3.93SLFSIDD346 pKa = 4.32DD347 pKa = 3.93PLYY350 pKa = 10.48TEE352 pKa = 4.51

Molecular weight: 40.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI50|A0A0B4UI50_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-28 OX=1592095 PE=3 SV=1
MM1 pKa = 7.68SYY3 pKa = 10.45LGKK6 pKa = 10.43RR7 pKa = 11.84KK8 pKa = 7.22MTSYY12 pKa = 8.5PTARR16 pKa = 11.84KK17 pKa = 9.54VSRR20 pKa = 11.84QRR22 pKa = 11.84SPLTLQYY29 pKa = 10.2GVPVTIPAGARR40 pKa = 11.84GYY42 pKa = 10.29VRR44 pKa = 11.84NQGRR48 pKa = 11.84FGRR51 pKa = 11.84TARR54 pKa = 11.84MQRR57 pKa = 11.84GDD59 pKa = 3.56SMPEE63 pKa = 3.51MKK65 pKa = 10.79YY66 pKa = 10.92LDD68 pKa = 3.78TSLTWSCDD76 pKa = 3.08TTGEE80 pKa = 4.11VSAPYY85 pKa = 10.73LNIIPQGVGEE95 pKa = 4.1NQRR98 pKa = 11.84VGRR101 pKa = 11.84KK102 pKa = 7.99VVVRR106 pKa = 11.84SIGIKK111 pKa = 10.45GLVVPLNTSVDD122 pKa = 3.17ASLIKK127 pKa = 10.36IQVVLDD133 pKa = 3.67TQANGTAPTFGGSADD148 pKa = 4.62SIWEE152 pKa = 4.31SNSIHH157 pKa = 6.33SWRR160 pKa = 11.84NPDD163 pKa = 2.73NAARR167 pKa = 11.84FKK169 pKa = 10.81VIKK172 pKa = 10.39EE173 pKa = 3.9FDD175 pKa = 3.7LKK177 pKa = 10.33PTVTAAYY184 pKa = 9.64YY185 pKa = 10.88NSTTTTEE192 pKa = 3.87KK193 pKa = 10.78RR194 pKa = 11.84LTNILRR200 pKa = 11.84VEE202 pKa = 4.0WFKK205 pKa = 11.55KK206 pKa = 9.79CFIPIEE212 pKa = 4.17YY213 pKa = 10.33DD214 pKa = 3.44SVLTTGALTTVRR226 pKa = 11.84SNNLLITCINSANDD240 pKa = 3.27DD241 pKa = 3.78QYY243 pKa = 11.78NFTGMQRR250 pKa = 11.84IRR252 pKa = 11.84YY253 pKa = 6.81TDD255 pKa = 2.88AA256 pKa = 5.83

Molecular weight: 28.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

608

256

352

304.0

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.072 ± 0.474

1.151 ± 0.012

5.921 ± 0.712

4.77 ± 0.949

3.618 ± 0.51

5.592 ± 0.83

1.316 ± 0.534

4.77 ± 0.854

6.414 ± 0.771

9.046 ± 1.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.809 ± 0.308

3.947 ± 1.103

6.414 ± 0.996

4.441 ± 0.308

6.579 ± 0.712

6.579 ± 0.712

6.908 ± 2.099

7.566 ± 0.142

2.138 ± 0.332

3.947 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski