Amino acid dipepetide frequency for Shigella phage Buco

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.355AlaAla: 15.355 ± 1.488
0.684AlaCys: 0.684 ± 0.324
5.549AlaAsp: 5.549 ± 0.745
4.941AlaGlu: 4.941 ± 0.774
2.889AlaPhe: 2.889 ± 0.462
8.438AlaGly: 8.438 ± 1.171
2.204AlaHis: 2.204 ± 0.515
4.181AlaIle: 4.181 ± 0.594
5.017AlaLys: 5.017 ± 0.743
9.426AlaLeu: 9.426 ± 0.884
3.117AlaMet: 3.117 ± 0.436
4.713AlaAsn: 4.713 ± 0.675
5.017AlaPro: 5.017 ± 1.072
6.842AlaGln: 6.842 ± 0.755
5.017AlaArg: 5.017 ± 0.771
6.233AlaSer: 6.233 ± 1.14
6.233AlaThr: 6.233 ± 0.595
6.005AlaVal: 6.005 ± 0.71
1.064AlaTrp: 1.064 ± 0.34
4.485AlaTyr: 4.485 ± 0.466
0.0AlaXaa: 0.0 ± 0.0
Cys
0.456CysAla: 0.456 ± 0.224
0.38CysCys: 0.38 ± 0.205
0.608CysAsp: 0.608 ± 0.231
0.532CysGlu: 0.532 ± 0.23
0.836CysPhe: 0.836 ± 0.269
0.608CysGly: 0.608 ± 0.174
0.456CysHis: 0.456 ± 0.197
0.836CysIle: 0.836 ± 0.322
0.38CysLys: 0.38 ± 0.238
0.608CysLeu: 0.608 ± 0.189
0.532CysMet: 0.532 ± 0.166
0.456CysAsn: 0.456 ± 0.201
0.532CysPro: 0.532 ± 0.228
0.304CysGln: 0.304 ± 0.16
0.304CysArg: 0.304 ± 0.183
0.608CysSer: 0.608 ± 0.215
0.76CysThr: 0.76 ± 0.233
0.684CysVal: 0.684 ± 0.22
0.076CysTrp: 0.076 ± 0.077
0.304CysTyr: 0.304 ± 0.148
0.0CysXaa: 0.0 ± 0.0
Asp
6.842AspAla: 6.842 ± 0.915
1.14AspCys: 1.14 ± 0.448
3.801AspAsp: 3.801 ± 0.544
3.573AspGlu: 3.573 ± 0.663
1.976AspPhe: 1.976 ± 0.346
4.789AspGly: 4.789 ± 0.667
0.608AspHis: 0.608 ± 0.192
3.269AspIle: 3.269 ± 0.451
2.661AspLys: 2.661 ± 0.532
5.473AspLeu: 5.473 ± 0.718
1.9AspMet: 1.9 ± 0.352
2.965AspAsn: 2.965 ± 0.435
2.661AspPro: 2.661 ± 0.335
1.824AspGln: 1.824 ± 0.372
2.433AspArg: 2.433 ± 0.436
5.093AspSer: 5.093 ± 0.753
3.269AspThr: 3.269 ± 0.533
3.421AspVal: 3.421 ± 0.516
0.988AspTrp: 0.988 ± 0.294
3.269AspTyr: 3.269 ± 0.548
0.0AspXaa: 0.0 ± 0.0
Glu
6.233GluAla: 6.233 ± 0.798
0.608GluCys: 0.608 ± 0.256
3.269GluAsp: 3.269 ± 0.46
3.725GluGlu: 3.725 ± 0.851
2.128GluPhe: 2.128 ± 0.418
3.269GluGly: 3.269 ± 0.362
1.824GluHis: 1.824 ± 0.379
2.509GluIle: 2.509 ± 0.541
2.357GluLys: 2.357 ± 0.621
5.625GluLeu: 5.625 ± 0.726
1.672GluMet: 1.672 ± 0.392
2.128GluAsn: 2.128 ± 0.456
1.368GluPro: 1.368 ± 0.41
3.573GluGln: 3.573 ± 0.514
3.801GluArg: 3.801 ± 0.628
2.889GluSer: 2.889 ± 0.532
2.813GluThr: 2.813 ± 0.363
4.257GluVal: 4.257 ± 0.819
0.912GluTrp: 0.912 ± 0.229
3.269GluTyr: 3.269 ± 0.419
0.0GluXaa: 0.0 ± 0.0
Phe
2.965PheAla: 2.965 ± 0.518
0.228PheCys: 0.228 ± 0.147
1.9PheAsp: 1.9 ± 0.337
1.52PheGlu: 1.52 ± 0.245
0.988PhePhe: 0.988 ± 0.274
1.976PheGly: 1.976 ± 0.283
0.532PheHis: 0.532 ± 0.228
1.444PheIle: 1.444 ± 0.371
1.824PheLys: 1.824 ± 0.366
2.204PheLeu: 2.204 ± 0.378
0.836PheMet: 0.836 ± 0.194
1.672PheAsn: 1.672 ± 0.35
0.912PhePro: 0.912 ± 0.296
1.52PheGln: 1.52 ± 0.328
2.433PheArg: 2.433 ± 0.472
2.052PheSer: 2.052 ± 0.501
1.596PheThr: 1.596 ± 0.302
1.444PheVal: 1.444 ± 0.339
0.38PheTrp: 0.38 ± 0.18
0.836PheTyr: 0.836 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
7.374GlyAla: 7.374 ± 0.686
1.292GlyCys: 1.292 ± 0.34
5.245GlyAsp: 5.245 ± 0.647
3.725GlyGlu: 3.725 ± 0.388
2.585GlyPhe: 2.585 ± 0.456
5.093GlyGly: 5.093 ± 0.843
1.292GlyHis: 1.292 ± 0.319
4.637GlyIle: 4.637 ± 0.561
4.333GlyLys: 4.333 ± 0.72
6.461GlyLeu: 6.461 ± 0.614
2.357GlyMet: 2.357 ± 0.368
3.497GlyAsn: 3.497 ± 0.678
1.368GlyPro: 1.368 ± 0.32
3.421GlyGln: 3.421 ± 0.453
4.561GlyArg: 4.561 ± 0.6
4.029GlySer: 4.029 ± 0.536
5.169GlyThr: 5.169 ± 0.65
5.473GlyVal: 5.473 ± 0.608
1.368GlyTrp: 1.368 ± 0.43
2.965GlyTyr: 2.965 ± 0.483
0.0GlyXaa: 0.0 ± 0.0
His
1.444HisAla: 1.444 ± 0.43
0.228HisCys: 0.228 ± 0.127
1.292HisAsp: 1.292 ± 0.308
1.14HisGlu: 1.14 ± 0.363
0.608HisPhe: 0.608 ± 0.225
2.128HisGly: 2.128 ± 0.655
0.228HisHis: 0.228 ± 0.128
0.684HisIle: 0.684 ± 0.18
0.836HisLys: 0.836 ± 0.261
3.193HisLeu: 3.193 ± 0.587
0.456HisMet: 0.456 ± 0.165
0.152HisAsn: 0.152 ± 0.117
0.456HisPro: 0.456 ± 0.251
0.836HisGln: 0.836 ± 0.335
1.748HisArg: 1.748 ± 0.357
1.216HisSer: 1.216 ± 0.236
0.988HisThr: 0.988 ± 0.324
0.608HisVal: 0.608 ± 0.234
0.608HisTrp: 0.608 ± 0.219
0.608HisTyr: 0.608 ± 0.294
0.0HisXaa: 0.0 ± 0.0
Ile
4.561IleAla: 4.561 ± 0.58
0.304IleCys: 0.304 ± 0.172
3.497IleAsp: 3.497 ± 0.591
3.345IleGlu: 3.345 ± 0.696
0.988IlePhe: 0.988 ± 0.284
2.204IleGly: 2.204 ± 0.404
0.608IleHis: 0.608 ± 0.199
2.433IleIle: 2.433 ± 0.44
2.433IleLys: 2.433 ± 0.385
4.333IleLeu: 4.333 ± 0.686
1.292IleMet: 1.292 ± 0.255
2.281IleAsn: 2.281 ± 0.409
2.813IlePro: 2.813 ± 0.5
2.052IleGln: 2.052 ± 0.387
2.965IleArg: 2.965 ± 0.429
2.357IleSer: 2.357 ± 0.318
2.965IleThr: 2.965 ± 0.535
2.509IleVal: 2.509 ± 0.456
0.532IleTrp: 0.532 ± 0.189
1.292IleTyr: 1.292 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
6.081LysAla: 6.081 ± 0.82
0.228LysCys: 0.228 ± 0.126
2.889LysAsp: 2.889 ± 0.384
4.713LysGlu: 4.713 ± 0.575
1.14LysPhe: 1.14 ± 0.286
3.573LysGly: 3.573 ± 0.557
0.532LysHis: 0.532 ± 0.22
1.292LysIle: 1.292 ± 0.347
1.976LysLys: 1.976 ± 0.397
5.245LysLeu: 5.245 ± 0.688
1.216LysMet: 1.216 ± 0.282
1.444LysAsn: 1.444 ± 0.311
2.204LysPro: 2.204 ± 0.369
2.281LysGln: 2.281 ± 0.367
3.041LysArg: 3.041 ± 0.513
2.509LysSer: 2.509 ± 0.439
1.976LysThr: 1.976 ± 0.265
3.953LysVal: 3.953 ± 0.506
0.988LysTrp: 0.988 ± 0.232
1.14LysTyr: 1.14 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
9.35LeuAla: 9.35 ± 0.8
1.368LeuCys: 1.368 ± 0.325
7.07LeuAsp: 7.07 ± 0.784
4.865LeuGlu: 4.865 ± 0.643
2.357LeuPhe: 2.357 ± 0.44
6.689LeuGly: 6.689 ± 0.654
1.9LeuHis: 1.9 ± 0.331
4.333LeuIle: 4.333 ± 0.701
3.877LeuLys: 3.877 ± 0.484
6.537LeuLeu: 6.537 ± 0.565
2.737LeuMet: 2.737 ± 0.452
4.029LeuAsn: 4.029 ± 0.493
4.181LeuPro: 4.181 ± 0.582
4.105LeuGln: 4.105 ± 0.475
5.169LeuArg: 5.169 ± 0.592
4.561LeuSer: 4.561 ± 0.591
4.941LeuThr: 4.941 ± 0.722
6.613LeuVal: 6.613 ± 0.705
1.064LeuTrp: 1.064 ± 0.287
3.421LeuTyr: 3.421 ± 0.523
0.0LeuXaa: 0.0 ± 0.0
Met
3.497MetAla: 3.497 ± 0.62
0.228MetCys: 0.228 ± 0.128
2.204MetAsp: 2.204 ± 0.364
1.064MetGlu: 1.064 ± 0.224
1.064MetPhe: 1.064 ± 0.281
1.824MetGly: 1.824 ± 0.366
0.608MetHis: 0.608 ± 0.219
0.684MetIle: 0.684 ± 0.208
0.912MetLys: 0.912 ± 0.355
3.953MetLeu: 3.953 ± 0.574
0.532MetMet: 0.532 ± 0.212
1.368MetAsn: 1.368 ± 0.295
1.14MetPro: 1.14 ± 0.275
1.976MetGln: 1.976 ± 0.281
2.128MetArg: 2.128 ± 0.41
1.976MetSer: 1.976 ± 0.46
1.216MetThr: 1.216 ± 0.42
1.368MetVal: 1.368 ± 0.332
0.38MetTrp: 0.38 ± 0.147
1.064MetTyr: 1.064 ± 0.38
0.0MetXaa: 0.0 ± 0.0
Asn
4.561AsnAla: 4.561 ± 0.536
0.076AsnCys: 0.076 ± 0.072
1.748AsnAsp: 1.748 ± 0.465
2.052AsnGlu: 2.052 ± 0.389
0.836AsnPhe: 0.836 ± 0.292
4.029AsnGly: 4.029 ± 0.669
0.684AsnHis: 0.684 ± 0.265
2.509AsnIle: 2.509 ± 0.522
2.281AsnLys: 2.281 ± 0.4
3.345AsnLeu: 3.345 ± 0.391
1.064AsnMet: 1.064 ± 0.318
2.128AsnAsn: 2.128 ± 0.679
3.497AsnPro: 3.497 ± 0.457
1.52AsnGln: 1.52 ± 0.302
2.128AsnArg: 2.128 ± 0.401
3.193AsnSer: 3.193 ± 0.546
2.965AsnThr: 2.965 ± 0.538
2.661AsnVal: 2.661 ± 0.555
0.38AsnTrp: 0.38 ± 0.161
1.064AsnTyr: 1.064 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
4.789ProAla: 4.789 ± 0.752
0.152ProCys: 0.152 ± 0.113
3.497ProAsp: 3.497 ± 0.429
3.649ProGlu: 3.649 ± 0.574
0.912ProPhe: 0.912 ± 0.228
3.117ProGly: 3.117 ± 0.447
0.304ProHis: 0.304 ± 0.141
1.368ProIle: 1.368 ± 0.321
1.444ProLys: 1.444 ± 0.353
2.813ProLeu: 2.813 ± 0.507
1.672ProMet: 1.672 ± 0.391
1.292ProAsn: 1.292 ± 0.264
1.064ProPro: 1.064 ± 0.275
1.52ProGln: 1.52 ± 0.386
1.748ProArg: 1.748 ± 0.372
1.976ProSer: 1.976 ± 0.337
2.357ProThr: 2.357 ± 0.448
3.725ProVal: 3.725 ± 0.575
0.608ProTrp: 0.608 ± 0.202
1.064ProTyr: 1.064 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
5.397GlnAla: 5.397 ± 0.729
0.304GlnCys: 0.304 ± 0.195
1.596GlnAsp: 1.596 ± 0.338
2.813GlnGlu: 2.813 ± 0.489
1.824GlnPhe: 1.824 ± 0.371
3.801GlnGly: 3.801 ± 0.375
1.14GlnHis: 1.14 ± 0.375
2.052GlnIle: 2.052 ± 0.425
2.357GlnLys: 2.357 ± 0.466
3.877GlnLeu: 3.877 ± 0.472
1.52GlnMet: 1.52 ± 0.353
2.509GlnAsn: 2.509 ± 0.542
1.748GlnPro: 1.748 ± 0.342
2.813GlnGln: 2.813 ± 0.798
3.345GlnArg: 3.345 ± 0.425
2.813GlnSer: 2.813 ± 0.464
2.281GlnThr: 2.281 ± 0.517
3.421GlnVal: 3.421 ± 0.474
0.836GlnTrp: 0.836 ± 0.294
1.672GlnTyr: 1.672 ± 0.298
0.0GlnXaa: 0.0 ± 0.0
Arg
5.701ArgAla: 5.701 ± 0.61
0.532ArgCys: 0.532 ± 0.25
3.193ArgAsp: 3.193 ± 0.441
3.649ArgGlu: 3.649 ± 0.513
2.052ArgPhe: 2.052 ± 0.322
4.029ArgGly: 4.029 ± 0.65
1.14ArgHis: 1.14 ± 0.242
2.889ArgIle: 2.889 ± 0.598
3.269ArgLys: 3.269 ± 0.443
5.017ArgLeu: 5.017 ± 0.51
1.672ArgMet: 1.672 ± 0.354
2.128ArgAsn: 2.128 ± 0.414
1.292ArgPro: 1.292 ± 0.294
2.661ArgGln: 2.661 ± 0.515
4.029ArgArg: 4.029 ± 0.799
3.041ArgSer: 3.041 ± 0.358
3.421ArgThr: 3.421 ± 0.444
4.333ArgVal: 4.333 ± 0.604
0.76ArgTrp: 0.76 ± 0.224
2.204ArgTyr: 2.204 ± 0.443
0.0ArgXaa: 0.0 ± 0.0
Ser
6.005SerAla: 6.005 ± 0.491
0.608SerCys: 0.608 ± 0.19
3.345SerAsp: 3.345 ± 0.541
3.573SerGlu: 3.573 ± 0.548
1.596SerPhe: 1.596 ± 0.369
5.853SerGly: 5.853 ± 0.804
0.988SerHis: 0.988 ± 0.248
3.573SerIle: 3.573 ± 0.688
3.269SerLys: 3.269 ± 0.549
5.245SerLeu: 5.245 ± 0.677
2.585SerMet: 2.585 ± 0.297
2.737SerAsn: 2.737 ± 0.434
2.509SerPro: 2.509 ± 0.426
2.128SerGln: 2.128 ± 0.414
2.509SerArg: 2.509 ± 0.439
3.953SerSer: 3.953 ± 0.706
3.801SerThr: 3.801 ± 0.65
4.105SerVal: 4.105 ± 0.528
1.064SerTrp: 1.064 ± 0.329
1.976SerTyr: 1.976 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
5.929ThrAla: 5.929 ± 0.752
0.608ThrCys: 0.608 ± 0.214
3.649ThrAsp: 3.649 ± 0.513
3.193ThrGlu: 3.193 ± 0.536
1.444ThrPhe: 1.444 ± 0.267
5.473ThrGly: 5.473 ± 0.832
1.292ThrHis: 1.292 ± 0.298
2.128ThrIle: 2.128 ± 0.405
2.737ThrLys: 2.737 ± 0.483
4.181ThrLeu: 4.181 ± 0.747
1.14ThrMet: 1.14 ± 0.329
2.433ThrAsn: 2.433 ± 0.579
2.281ThrPro: 2.281 ± 0.363
1.596ThrGln: 1.596 ± 0.285
2.737ThrArg: 2.737 ± 0.488
4.029ThrSer: 4.029 ± 0.579
2.813ThrThr: 2.813 ± 0.647
4.637ThrVal: 4.637 ± 0.642
0.608ThrTrp: 0.608 ± 0.199
2.585ThrTyr: 2.585 ± 0.555
0.0ThrXaa: 0.0 ± 0.0
Val
6.613ValAla: 6.613 ± 0.882
0.684ValCys: 0.684 ± 0.212
4.409ValAsp: 4.409 ± 0.704
2.813ValGlu: 2.813 ± 0.655
1.748ValPhe: 1.748 ± 0.37
5.549ValGly: 5.549 ± 0.678
2.052ValHis: 2.052 ± 0.421
2.204ValIle: 2.204 ± 0.424
3.649ValLys: 3.649 ± 0.614
6.689ValLeu: 6.689 ± 1.028
1.748ValMet: 1.748 ± 0.333
2.204ValAsn: 2.204 ± 0.447
2.585ValPro: 2.585 ± 0.536
4.333ValGln: 4.333 ± 0.464
4.029ValArg: 4.029 ± 0.442
4.941ValSer: 4.941 ± 0.661
3.421ValThr: 3.421 ± 0.699
5.473ValVal: 5.473 ± 0.688
0.76ValTrp: 0.76 ± 0.276
2.585ValTyr: 2.585 ± 0.572
0.0ValXaa: 0.0 ± 0.0
Trp
0.988TrpAla: 0.988 ± 0.211
0.228TrpCys: 0.228 ± 0.116
0.988TrpAsp: 0.988 ± 0.251
0.988TrpGlu: 0.988 ± 0.222
0.532TrpPhe: 0.532 ± 0.211
1.064TrpGly: 1.064 ± 0.331
0.304TrpHis: 0.304 ± 0.135
0.456TrpIle: 0.456 ± 0.167
0.836TrpLys: 0.836 ± 0.257
1.216TrpLeu: 1.216 ± 0.286
0.228TrpMet: 0.228 ± 0.123
0.684TrpAsn: 0.684 ± 0.2
0.304TrpPro: 0.304 ± 0.173
0.38TrpGln: 0.38 ± 0.158
0.684TrpArg: 0.684 ± 0.27
0.836TrpSer: 0.836 ± 0.276
0.836TrpThr: 0.836 ± 0.277
1.444TrpVal: 1.444 ± 0.326
0.228TrpTrp: 0.228 ± 0.129
0.912TrpTyr: 0.912 ± 0.317
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.269TyrAla: 3.269 ± 0.457
0.38TyrCys: 0.38 ± 0.175
2.204TyrAsp: 2.204 ± 0.419
2.357TyrGlu: 2.357 ± 0.381
0.76TyrPhe: 0.76 ± 0.268
2.813TyrGly: 2.813 ± 0.638
0.76TyrHis: 0.76 ± 0.264
2.281TyrIle: 2.281 ± 0.538
1.976TyrLys: 1.976 ± 0.355
3.725TyrLeu: 3.725 ± 0.503
0.836TyrMet: 0.836 ± 0.277
1.9TyrAsn: 1.9 ± 0.224
1.14TyrPro: 1.14 ± 0.257
2.433TyrGln: 2.433 ± 0.492
2.204TyrArg: 2.204 ± 0.543
3.117TyrSer: 3.117 ± 0.465
1.672TyrThr: 1.672 ± 0.391
2.357TyrVal: 2.357 ± 0.354
0.532TyrTrp: 0.532 ± 0.177
1.444TyrTyr: 1.444 ± 0.437
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (13156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski