Gordonia phage BrutonGaster

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Oneupvirus; Gordonia virus BrutonGaster

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JME8|A0A482JME8_9CAUD Uncharacterized protein OS=Gordonia phage BrutonGaster OX=2530116 GN=8 PE=4 SV=1
MM1 pKa = 7.55SEE3 pKa = 4.36YY4 pKa = 10.2IEE6 pKa = 4.37PTTGRR11 pKa = 11.84EE12 pKa = 3.55LDD14 pKa = 3.67SYY16 pKa = 11.26DD17 pKa = 3.46LHH19 pKa = 8.54EE20 pKa = 4.9MFDD23 pKa = 3.66EE24 pKa = 5.25FVDD27 pKa = 4.93EE28 pKa = 4.22IQPAVEE34 pKa = 4.0ILGIHH39 pKa = 5.87YY40 pKa = 8.91NASLVLKK47 pKa = 9.35EE48 pKa = 4.44VSTIDD53 pKa = 3.84YY54 pKa = 9.88QIQYY58 pKa = 10.61NDD60 pKa = 3.36WLDD63 pKa = 3.56ARR65 pKa = 11.84ISDD68 pKa = 4.32GNIVPANEE76 pKa = 3.95YY77 pKa = 10.65EE78 pKa = 4.21RR79 pKa = 11.84DD80 pKa = 3.63EE81 pKa = 4.41VDD83 pKa = 2.93AA84 pKa = 5.81

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JN52|A0A482JN52_9CAUD Uncharacterized protein OS=Gordonia phage BrutonGaster OX=2530116 GN=57 PE=4 SV=1
MM1 pKa = 7.08YY2 pKa = 10.37VVNPCKK8 pKa = 10.3QCGADD13 pKa = 3.71RR14 pKa = 11.84NSLEE18 pKa = 4.22STADD22 pKa = 3.44AAKK25 pKa = 9.29TCQLCRR31 pKa = 11.84RR32 pKa = 11.84AYY34 pKa = 8.67QYY36 pKa = 10.93RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84SQRR42 pKa = 11.84KK43 pKa = 8.81CSVEE47 pKa = 3.71GCARR51 pKa = 11.84LCNNVRR57 pKa = 11.84FCYY60 pKa = 9.91VHH62 pKa = 6.82ARR64 pKa = 11.84VQTEE68 pKa = 3.79EE69 pKa = 4.19AQTCSVNDD77 pKa = 3.55CLKK80 pKa = 10.37RR81 pKa = 11.84VQAKK85 pKa = 8.72GLCSSHH91 pKa = 5.52YY92 pKa = 8.25QASRR96 pKa = 11.84RR97 pKa = 11.84EE98 pKa = 4.15AVAKK102 pKa = 10.02RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84QIGCRR110 pKa = 11.84VSGCPKK116 pKa = 9.93PLLAKK121 pKa = 9.56GLCKK125 pKa = 10.1SHH127 pKa = 6.65YY128 pKa = 9.53DD129 pKa = 3.14ATRR132 pKa = 11.84RR133 pKa = 11.84EE134 pKa = 4.03EE135 pKa = 3.86LLEE138 pKa = 3.84VEE140 pKa = 4.61RR141 pKa = 11.84RR142 pKa = 11.84WRR144 pKa = 11.84QKK146 pKa = 10.58NRR148 pKa = 11.84DD149 pKa = 3.34KK150 pKa = 11.2VRR152 pKa = 11.84EE153 pKa = 4.04KK154 pKa = 10.21IRR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84QARR161 pKa = 11.84KK162 pKa = 9.58RR163 pKa = 11.84EE164 pKa = 3.83LDD166 pKa = 3.3VRR168 pKa = 11.84AVADD172 pKa = 4.12RR173 pKa = 11.84DD174 pKa = 3.7LLRR177 pKa = 11.84LRR179 pKa = 11.84NRR181 pKa = 11.84QLGRR185 pKa = 11.84CFYY188 pKa = 10.99CDD190 pKa = 3.44DD191 pKa = 4.07RR192 pKa = 11.84LAAGQEE198 pKa = 4.47HH199 pKa = 6.9VDD201 pKa = 3.42HH202 pKa = 6.2VVPMSRR208 pKa = 11.84GGRR211 pKa = 11.84HH212 pKa = 5.95SIGNLVIACAGCNCSKK228 pKa = 10.97SDD230 pKa = 2.93WFLYY234 pKa = 8.78EE235 pKa = 3.63WKK237 pKa = 10.66LNQTTGRR244 pKa = 11.84PLIRR248 pKa = 11.84RR249 pKa = 11.84SRR251 pKa = 11.84APVV254 pKa = 2.9

Molecular weight: 29.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

28710

35

2839

173.0

19.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.321 ± 0.373

1.087 ± 0.159

6.984 ± 0.189

7.057 ± 0.235

2.999 ± 0.115

7.84 ± 0.299

2.25 ± 0.157

4.988 ± 0.14

4.587 ± 0.248

7.736 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.108

3.852 ± 0.146

4.709 ± 0.181

3.647 ± 0.178

6.433 ± 0.193

5.566 ± 0.163

6.099 ± 0.196

7.029 ± 0.263

2.118 ± 0.101

3.197 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski