Amino acid dipepetide frequency for Streptomyces sp. NRRL F-4428

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.985AlaAla: 23.985 ± 0.201	1.094AlaCys: 1.094 ± 0.028	8.652AlaAsp: 8.652 ± 0.082	9.278AlaGlu: 9.278 ± 0.114	3.618AlaPhe: 3.618 ± 0.051	14.262AlaGly: 14.262 ± 0.105	2.947AlaHis: 2.947 ± 0.055	3.277AlaIle: 3.277 ± 0.049	3.068AlaLys: 3.068 ± 0.057	14.722AlaLeu: 14.722 ± 0.114	2.655AlaMet: 2.655 ± 0.042	1.956AlaAsn: 1.956 ± 0.033	7.746AlaPro: 7.746 ± 0.099	3.902AlaGln: 3.902 ± 0.049	10.497AlaArg: 10.497 ± 0.108	5.802AlaSer: 5.802 ± 0.052	6.815AlaThr: 6.815 ± 0.071	12.856AlaVal: 12.856 ± 0.106	1.912AlaTrp: 1.912 ± 0.039	2.831AlaTyr: 2.831 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.138CysAla: 1.138 ± 0.025	0.095CysCys: 0.095 ± 0.008	0.461CysAsp: 0.461 ± 0.016	0.407CysGlu: 0.407 ± 0.015	0.215CysPhe: 0.215 ± 0.01	0.999CysGly: 0.999 ± 0.021	0.188CysHis: 0.188 ± 0.01	0.175CysIle: 0.175 ± 0.01	0.122CysLys: 0.122 ± 0.008	0.757CysLeu: 0.757 ± 0.02	0.139CysMet: 0.139 ± 0.009	0.141CysAsn: 0.141 ± 0.009	0.476CysPro: 0.476 ± 0.015	0.156CysGln: 0.156 ± 0.011	0.618CysArg: 0.618 ± 0.022	0.404CysSer: 0.404 ± 0.016	0.534CysThr: 0.534 ± 0.017	0.673CysVal: 0.673 ± 0.018	0.122CysTrp: 0.122 ± 0.009	0.141CysTyr: 0.141 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.684AspAla: 7.684 ± 0.073	0.47AspCys: 0.47 ± 0.015	3.152AspAsp: 3.152 ± 0.049	3.774AspGlu: 3.774 ± 0.046	1.669AspPhe: 1.669 ± 0.029	6.326AspGly: 6.326 ± 0.072	1.385AspHis: 1.385 ± 0.029	1.817AspIle: 1.817 ± 0.032	1.233AspLys: 1.233 ± 0.03	6.177AspLeu: 6.177 ± 0.069	0.854AspMet: 0.854 ± 0.026	0.922AspAsn: 0.922 ± 0.024	4.598AspPro: 4.598 ± 0.055	1.458AspGln: 1.458 ± 0.031	4.873AspArg: 4.873 ± 0.059	2.395AspSer: 2.395 ± 0.036	3.123AspThr: 3.123 ± 0.044	4.53AspVal: 4.53 ± 0.057	1.002AspTrp: 1.002 ± 0.024	1.076AspTyr: 1.076 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	8.084GluAla: 8.084 ± 0.106	0.387GluCys: 0.387 ± 0.015	2.883GluAsp: 2.883 ± 0.042	3.668GluGlu: 3.668 ± 0.06	1.512GluPhe: 1.512 ± 0.03	4.528GluGly: 4.528 ± 0.05	1.525GluHis: 1.525 ± 0.029	2.315GluIle: 2.315 ± 0.038	1.472GluLys: 1.472 ± 0.031	6.889GluLeu: 6.889 ± 0.072	0.924GluMet: 0.924 ± 0.026	1.046GluAsn: 1.046 ± 0.025	3.374GluPro: 3.374 ± 0.048	2.231GluGln: 2.231 ± 0.037	5.312GluArg: 5.312 ± 0.069	2.476GluSer: 2.476 ± 0.041	2.694GluThr: 2.694 ± 0.036	4.587GluVal: 4.587 ± 0.053	0.743GluTrp: 0.743 ± 0.022	1.184GluTyr: 1.184 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.769PheAla: 3.769 ± 0.05	0.28PheCys: 0.28 ± 0.013	1.921PheAsp: 1.921 ± 0.034	1.494PheGlu: 1.494 ± 0.031	0.875PhePhe: 0.875 ± 0.027	3.074PheGly: 3.074 ± 0.047	0.59PheHis: 0.59 ± 0.017	0.692PheIle: 0.692 ± 0.02	0.575PheLys: 0.575 ± 0.018	2.522PheLeu: 2.522 ± 0.041	0.426PheMet: 0.426 ± 0.016	0.581PheAsn: 0.581 ± 0.021	1.407PhePro: 1.407 ± 0.028	0.63PheGln: 0.63 ± 0.019	1.859PheArg: 1.859 ± 0.029	1.445PheSer: 1.445 ± 0.032	2.036PheThr: 2.036 ± 0.034	2.166PheVal: 2.166 ± 0.038	0.416PheTrp: 0.416 ± 0.016	0.573PheTyr: 0.573 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	11.779GlyAla: 11.779 ± 0.109	0.849GlyCys: 0.849 ± 0.024	5.088GlyAsp: 5.088 ± 0.055	5.237GlyGlu: 5.237 ± 0.06	2.962GlyPhe: 2.962 ± 0.048	9.432GlyGly: 9.432 ± 0.098	2.304GlyHis: 2.304 ± 0.039	3.547GlyIle: 3.547 ± 0.049	2.467GlyLys: 2.467 ± 0.043	9.67GlyLeu: 9.67 ± 0.08	1.997GlyMet: 1.997 ± 0.03	1.775GlyAsn: 1.775 ± 0.032	5.58GlyPro: 5.58 ± 0.061	2.614GlyGln: 2.614 ± 0.045	8.079GlyArg: 8.079 ± 0.072	5.404GlySer: 5.404 ± 0.059	6.161GlyThr: 6.161 ± 0.065	7.457GlyVal: 7.457 ± 0.067	1.697GlyTrp: 1.697 ± 0.034	2.298GlyTyr: 2.298 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.721HisAla: 2.721 ± 0.045	0.207HisCys: 0.207 ± 0.01	1.306HisAsp: 1.306 ± 0.029	1.211HisGlu: 1.211 ± 0.026	0.613HisPhe: 0.613 ± 0.018	2.421HisGly: 2.421 ± 0.037	0.677HisHis: 0.677 ± 0.02	0.612HisIle: 0.612 ± 0.02	0.375HisLys: 0.375 ± 0.014	2.388HisLeu: 2.388 ± 0.041	0.358HisMet: 0.358 ± 0.015	0.368HisAsn: 0.368 ± 0.015	1.795HisPro: 1.795 ± 0.034	0.607HisGln: 0.607 ± 0.017	2.02HisArg: 2.02 ± 0.039	0.995HisSer: 0.995 ± 0.026	1.356HisThr: 1.356 ± 0.032	1.673HisVal: 1.673 ± 0.032	0.345HisTrp: 0.345 ± 0.015	0.466HisTyr: 0.466 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	4.684IleAla: 4.684 ± 0.053	0.282IleCys: 0.282 ± 0.011	2.107IleAsp: 2.107 ± 0.038	1.962IleGlu: 1.962 ± 0.037	0.685IlePhe: 0.685 ± 0.022	3.511IleGly: 3.511 ± 0.058	0.564IleHis: 0.564 ± 0.019	0.83IleIle: 0.83 ± 0.026	0.709IleLys: 0.709 ± 0.024	2.375IleLeu: 2.375 ± 0.042	0.454IleMet: 0.454 ± 0.016	0.68IleAsn: 0.68 ± 0.021	1.756IlePro: 1.756 ± 0.033	0.733IleGln: 0.733 ± 0.02	2.266IleArg: 2.266 ± 0.036	1.612IleSer: 1.612 ± 0.035	2.128IleThr: 2.128 ± 0.038	2.579IleVal: 2.579 ± 0.041	0.356IleTrp: 0.356 ± 0.016	0.49IleTyr: 0.49 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.175LysAla: 3.175 ± 0.051	0.12LysCys: 0.12 ± 0.009	1.368LysAsp: 1.368 ± 0.032	1.197LysGlu: 1.197 ± 0.029	0.474LysPhe: 0.474 ± 0.017	1.899LysGly: 1.899 ± 0.045	0.431LysHis: 0.431 ± 0.015	0.88LysIle: 0.88 ± 0.022	0.86LysLys: 0.86 ± 0.028	2.102LysLeu: 2.102 ± 0.034	0.408LysMet: 0.408 ± 0.015	0.535LysAsn: 0.535 ± 0.018	1.387LysPro: 1.387 ± 0.032	0.717LysGln: 0.717 ± 0.023	1.434LysArg: 1.434 ± 0.03	1.118LysSer: 1.118 ± 0.025	1.285LysThr: 1.285 ± 0.031	1.959LysVal: 1.959 ± 0.038	0.256LysTrp: 0.256 ± 0.013	0.462LysTyr: 0.462 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.619LeuAla: 15.619 ± 0.129	0.855LeuCys: 0.855 ± 0.022	6.633LeuAsp: 6.633 ± 0.081	4.841LeuGlu: 4.841 ± 0.059	2.575LeuPhe: 2.575 ± 0.044	9.399LeuGly: 9.399 ± 0.082	2.257LeuHis: 2.257 ± 0.035	3.04LeuIle: 3.04 ± 0.046	2.05LeuLys: 2.05 ± 0.041	11.368LeuLeu: 11.368 ± 0.114	1.614LeuMet: 1.614 ± 0.033	1.627LeuAsn: 1.627 ± 0.031	6.606LeuPro: 6.606 ± 0.064	2.22LeuGln: 2.22 ± 0.035	8.612LeuArg: 8.612 ± 0.081	5.107LeuSer: 5.107 ± 0.058	6.571LeuThr: 6.571 ± 0.068	8.907LeuVal: 8.907 ± 0.079	1.27LeuTrp: 1.27 ± 0.027	1.858LeuTyr: 1.858 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.395MetAla: 2.395 ± 0.039	0.143MetCys: 0.143 ± 0.009	0.953MetAsp: 0.953 ± 0.025	0.799MetGlu: 0.799 ± 0.023	0.454MetPhe: 0.454 ± 0.017	1.359MetGly: 1.359 ± 0.032	0.351MetHis: 0.351 ± 0.014	0.631MetIle: 0.631 ± 0.02	0.455MetLys: 0.455 ± 0.019	1.752MetLeu: 1.752 ± 0.032	0.295MetMet: 0.295 ± 0.013	0.435MetAsn: 0.435 ± 0.017	1.17MetPro: 1.17 ± 0.027	0.456MetGln: 0.456 ± 0.017	1.474MetArg: 1.474 ± 0.028	1.325MetSer: 1.325 ± 0.029	1.481MetThr: 1.481 ± 0.034	1.277MetVal: 1.277 ± 0.028	0.232MetTrp: 0.232 ± 0.011	0.324MetTyr: 0.324 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.157AsnAla: 2.157 ± 0.034	0.174AsnCys: 0.174 ± 0.009	0.901AsnAsp: 0.901 ± 0.022	0.838AsnGlu: 0.838 ± 0.022	0.5AsnPhe: 0.5 ± 0.019	1.783AsnGly: 1.783 ± 0.034	0.393AsnHis: 0.393 ± 0.015	0.645AsnIle: 0.645 ± 0.021	0.423AsnLys: 0.423 ± 0.017	1.701AsnLeu: 1.701 ± 0.03	0.321AsnMet: 0.321 ± 0.013	0.437AsnAsn: 0.437 ± 0.019	1.366AsnPro: 1.366 ± 0.033	0.493AsnGln: 0.493 ± 0.02	1.25AsnArg: 1.25 ± 0.025	0.863AsnSer: 0.863 ± 0.024	1.137AsnThr: 1.137 ± 0.026	1.353AsnVal: 1.353 ± 0.032	0.27AsnTrp: 0.27 ± 0.014	0.363AsnTyr: 0.363 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.781ProAla: 9.781 ± 0.119	0.333ProCys: 0.333 ± 0.012	4.429ProAsp: 4.429 ± 0.059	4.313ProGlu: 4.313 ± 0.055	1.532ProPhe: 1.532 ± 0.03	6.999ProGly: 6.999 ± 0.074	1.352ProHis: 1.352 ± 0.028	1.18ProIle: 1.18 ± 0.023	1.184ProLys: 1.184 ± 0.03	5.41ProLeu: 5.41 ± 0.06	1.021ProMet: 1.021 ± 0.025	0.893ProAsn: 0.893 ± 0.022	3.634ProPro: 3.634 ± 0.074	1.937ProGln: 1.937 ± 0.042	3.963ProArg: 3.963 ± 0.051	3.053ProSer: 3.053 ± 0.055	2.979ProThr: 2.979 ± 0.045	5.59ProVal: 5.59 ± 0.065	0.932ProTrp: 0.932 ± 0.026	1.466ProTyr: 1.466 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.849GlnAla: 3.849 ± 0.053	0.171GlnCys: 0.171 ± 0.01	1.477GlnAsp: 1.477 ± 0.031	1.497GlnGlu: 1.497 ± 0.032	0.643GlnPhe: 0.643 ± 0.017	2.32GlnGly: 2.32 ± 0.039	0.588GlnHis: 0.588 ± 0.02	1.07GlnIle: 1.07 ± 0.028	0.625GlnLys: 0.625 ± 0.019	2.916GlnLeu: 2.916 ± 0.036	0.525GlnMet: 0.525 ± 0.018	0.496GlnAsn: 0.496 ± 0.019	1.708GlnPro: 1.708 ± 0.034	1.209GlnGln: 1.209 ± 0.037	2.223GlnArg: 2.223 ± 0.034	1.233GlnSer: 1.233 ± 0.027	1.332GlnThr: 1.332 ± 0.029	2.39GlnVal: 2.39 ± 0.033	0.452GlnTrp: 0.452 ± 0.016	0.612GlnTyr: 0.612 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.968ArgAla: 9.968 ± 0.099	0.584ArgCys: 0.584 ± 0.019	4.122ArgAsp: 4.122 ± 0.062	4.85ArgGlu: 4.85 ± 0.056	2.37ArgPhe: 2.37 ± 0.04	5.963ArgGly: 5.963 ± 0.059	2.039ArgHis: 2.039 ± 0.04	3.354ArgIle: 3.354 ± 0.05	1.641ArgLys: 1.641 ± 0.037	8.701ArgLeu: 8.701 ± 0.1	1.802ArgMet: 1.802 ± 0.034	1.31ArgAsn: 1.31 ± 0.024	5.025ArgPro: 5.025 ± 0.063	2.188ArgGln: 2.188 ± 0.035	7.621ArgArg: 7.621 ± 0.095	3.956ArgSer: 3.956 ± 0.055	5.417ArgThr: 5.417 ± 0.058	5.718ArgVal: 5.718 ± 0.064	1.361ArgTrp: 1.361 ± 0.029	1.766ArgTyr: 1.766 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.805SerAla: 6.805 ± 0.063	0.418SerCys: 0.418 ± 0.016	2.509SerAsp: 2.509 ± 0.041	2.352SerGlu: 2.352 ± 0.037	1.51SerPhe: 1.51 ± 0.027	5.737SerGly: 5.737 ± 0.058	0.988SerHis: 0.988 ± 0.025	1.336SerIle: 1.336 ± 0.034	1.012SerLys: 1.012 ± 0.031	4.731SerLeu: 4.731 ± 0.056	1.051SerMet: 1.051 ± 0.025	0.829SerAsn: 0.829 ± 0.024	3.118SerPro: 3.118 ± 0.042	1.222SerGln: 1.222 ± 0.027	3.478SerArg: 3.478 ± 0.048	2.454SerSer: 2.454 ± 0.039	2.803SerThr: 2.803 ± 0.043	4.153SerVal: 4.153 ± 0.05	0.868SerTrp: 0.868 ± 0.025	1.215SerTyr: 1.215 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.087ThrAla: 9.087 ± 0.087	0.41ThrCys: 0.41 ± 0.016	3.391ThrAsp: 3.391 ± 0.046	3.12ThrGlu: 3.12 ± 0.04	1.504ThrPhe: 1.504 ± 0.031	6.656ThrGly: 6.656 ± 0.07	1.124ThrHis: 1.124 ± 0.025	1.555ThrIle: 1.555 ± 0.032	1.137ThrLys: 1.137 ± 0.028	5.531ThrLeu: 5.531 ± 0.054	0.905ThrMet: 0.905 ± 0.026	0.899ThrAsn: 0.899 ± 0.024	4.113ThrPro: 4.113 ± 0.058	1.329ThrGln: 1.329 ± 0.028	3.825ThrArg: 3.825 ± 0.053	2.826ThrSer: 2.826 ± 0.04	3.527ThrThr: 3.527 ± 0.057	5.851ThrVal: 5.851 ± 0.06	0.872ThrTrp: 0.872 ± 0.025	1.224ThrTyr: 1.224 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.638ValAla: 10.638 ± 0.084	0.754ValCys: 0.754 ± 0.023	4.926ValAsp: 4.926 ± 0.052	4.815ValGlu: 4.815 ± 0.056	2.473ValPhe: 2.473 ± 0.042	6.415ValGly: 6.415 ± 0.068	2.057ValHis: 2.057 ± 0.033	2.798ValIle: 2.798 ± 0.05	1.836ValLys: 1.836 ± 0.039	9.51ValLeu: 9.51 ± 0.095	1.445ValMet: 1.445 ± 0.031	1.682ValAsn: 1.682 ± 0.034	5.285ValPro: 5.285 ± 0.059	2.039ValGln: 2.039 ± 0.034	7.246ValArg: 7.246 ± 0.076	4.294ValSer: 4.294 ± 0.056	5.357ValThr: 5.357 ± 0.064	8.018ValVal: 8.018 ± 0.087	1.165ValTrp: 1.165 ± 0.026	1.601ValTyr: 1.601 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.702TrpAla: 1.702 ± 0.033	0.164TrpCys: 0.164 ± 0.01	0.802TrpAsp: 0.802 ± 0.02	0.751TrpGlu: 0.751 ± 0.025	0.5TrpPhe: 0.5 ± 0.018	1.034TrpGly: 1.034 ± 0.022	0.375TrpHis: 0.375 ± 0.015	0.564TrpIle: 0.564 ± 0.02	0.383TrpLys: 0.383 ± 0.015	1.757TrpLeu: 1.757 ± 0.038	0.299TrpMet: 0.299 ± 0.013	0.39TrpAsn: 0.39 ± 0.016	0.785TrpPro: 0.785 ± 0.021	0.617TrpGln: 0.617 ± 0.018	1.277TrpArg: 1.277 ± 0.03	0.881TrpSer: 0.881 ± 0.022	1.018TrpThr: 1.018 ± 0.026	0.969TrpVal: 0.969 ± 0.024	0.339TrpTrp: 0.339 ± 0.015	0.352TrpTyr: 0.352 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.853TyrAla: 2.853 ± 0.04	0.173TyrCys: 0.173 ± 0.01	1.437TyrAsp: 1.437 ± 0.027	1.432TyrGlu: 1.432 ± 0.03	0.648TyrPhe: 0.648 ± 0.02	2.281TyrGly: 2.281 ± 0.03	0.379TyrHis: 0.379 ± 0.015	0.448TyrIle: 0.448 ± 0.015	0.439TyrLys: 0.439 ± 0.016	2.051TyrLeu: 2.051 ± 0.033	0.267TyrMet: 0.267 ± 0.012	0.365TyrAsn: 0.365 ± 0.014	1.052TyrPro: 1.052 ± 0.027	0.576TyrGln: 0.576 ± 0.018	1.826TyrArg: 1.826 ± 0.036	0.945TyrSer: 0.945 ± 0.025	1.131TyrThr: 1.131 ± 0.029	1.649TyrVal: 1.649 ± 0.028	0.35TyrTrp: 0.35 ± 0.015	0.413TyrTyr: 0.413 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5663 proteins (1785054 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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