Clostridium estertheticum subsp. estertheticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium estertheticum

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GJD0|A0A1J0GJD0_9CLOT Uncharacterized protein OS=Clostridium estertheticum subsp. estertheticum OX=1552 GN=A7L45_15985 PE=4 SV=1
MM1 pKa = 7.25NKK3 pKa = 9.81QYY5 pKa = 10.71IVNFLSKK12 pKa = 11.01NDD14 pKa = 3.81LSDD17 pKa = 3.25IAEE20 pKa = 3.96IQYY23 pKa = 11.14KK24 pKa = 10.22KK25 pKa = 10.22GTIALKK31 pKa = 10.28FKK33 pKa = 10.8YY34 pKa = 10.29YY35 pKa = 10.66YY36 pKa = 10.74DD37 pKa = 3.95DD38 pKa = 5.13AEE40 pKa = 4.81IEE42 pKa = 4.11AAKK45 pKa = 9.86TYY47 pKa = 11.21ADD49 pKa = 3.83DD50 pKa = 3.93EE51 pKa = 5.42GNFKK55 pKa = 10.48EE56 pKa = 5.76EE57 pKa = 3.72KK58 pKa = 10.14DD59 pKa = 3.21DD60 pKa = 3.62WYY62 pKa = 10.74EE63 pKa = 4.12EE64 pKa = 3.97FFLPYY69 pKa = 10.41LSDD72 pKa = 3.36ISADD76 pKa = 3.43NVEE79 pKa = 5.71DD80 pKa = 3.78IMEE83 pKa = 4.3EE84 pKa = 4.12CAEE87 pKa = 3.97KK88 pKa = 10.5MEE90 pKa = 4.75IEE92 pKa = 4.35YY93 pKa = 10.85EE94 pKa = 4.42FIGCDD99 pKa = 3.06ISEE102 pKa = 4.57DD103 pKa = 3.29AYY105 pKa = 10.6EE106 pKa = 4.18FNEE109 pKa = 4.25FAVVFYY115 pKa = 10.76GKK117 pKa = 10.43EE118 pKa = 3.91SDD120 pKa = 4.31IDD122 pKa = 3.55TDD124 pKa = 5.49DD125 pKa = 4.05ILLEE129 pKa = 4.25LDD131 pKa = 4.0FF132 pKa = 6.71

Molecular weight: 15.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GLV0|A0A1J0GLV0_9CLOT 50S ribosomal protein L17 OS=Clostridium estertheticum subsp. estertheticum OX=1552 GN=rplQ PE=3 SV=1
MM1 pKa = 7.0EE2 pKa = 5.4AKK4 pKa = 10.46AIAKK8 pKa = 7.65YY9 pKa = 10.77VRR11 pKa = 11.84TSSMKK16 pKa = 9.11MGIVLGLIRR25 pKa = 11.84GKK27 pKa = 10.09NVNEE31 pKa = 4.02AFAILQYY38 pKa = 9.53TPKK41 pKa = 10.55NAAEE45 pKa = 4.25VVNKK49 pKa = 9.64VLKK52 pKa = 10.63SAVANAEE59 pKa = 3.99NNLNLDD65 pKa = 3.37INRR68 pKa = 11.84LYY70 pKa = 10.65VAEE73 pKa = 5.44AYY75 pKa = 9.97ACQGPTLKK83 pKa = 10.44RR84 pKa = 11.84FRR86 pKa = 11.84PRR88 pKa = 11.84AQGRR92 pKa = 11.84AYY94 pKa = 10.37RR95 pKa = 11.84IRR97 pKa = 11.84KK98 pKa = 9.0RR99 pKa = 11.84SSHH102 pKa = 3.99VTIIVTEE109 pKa = 4.46RR110 pKa = 11.84SS111 pKa = 3.31

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4203

0

4203

1303679

37

3075

310.2

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.736 ± 0.041

1.235 ± 0.015

5.406 ± 0.026

6.62 ± 0.043

4.376 ± 0.031

6.505 ± 0.039

1.387 ± 0.012

10.18 ± 0.049

9.013 ± 0.039

9.031 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.019

6.208 ± 0.037

2.785 ± 0.022

2.443 ± 0.017

3.162 ± 0.024

6.526 ± 0.035

5.314 ± 0.031

6.602 ± 0.035

0.706 ± 0.011

4.02 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski