Amino acid dipepetide frequency for Thermanaeromonas toyohensis ToBE

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.882AlaAla: 9.882 ± 0.138	1.284AlaCys: 1.284 ± 0.039	3.312AlaAsp: 3.312 ± 0.052	6.352AlaGlu: 6.352 ± 0.087	3.224AlaPhe: 3.224 ± 0.062	8.208AlaGly: 8.208 ± 0.103	1.513AlaHis: 1.513 ± 0.04	5.586AlaIle: 5.586 ± 0.084	4.116AlaLys: 4.116 ± 0.068	11.729AlaLeu: 11.729 ± 0.123	1.944AlaMet: 1.944 ± 0.047	2.014AlaAsn: 2.014 ± 0.049	3.136AlaPro: 3.136 ± 0.055	3.37AlaGln: 3.37 ± 0.065	6.838AlaArg: 6.838 ± 0.093	4.623AlaSer: 4.623 ± 0.075	4.117AlaThr: 4.117 ± 0.065	7.448AlaVal: 7.448 ± 0.099	1.216AlaTrp: 1.216 ± 0.041	2.634AlaTyr: 2.634 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.863CysAla: 0.863 ± 0.03	0.238CysCys: 0.238 ± 0.02	0.392CysAsp: 0.392 ± 0.022	0.547CysGlu: 0.547 ± 0.024	0.376CysPhe: 0.376 ± 0.019	1.429CysGly: 1.429 ± 0.047	0.299CysHis: 0.299 ± 0.017	0.552CysIle: 0.552 ± 0.024	0.367CysLys: 0.367 ± 0.019	1.324CysLeu: 1.324 ± 0.037	0.22CysMet: 0.22 ± 0.016	0.337CysAsn: 0.337 ± 0.019	0.936CysPro: 0.936 ± 0.032	0.48CysGln: 0.48 ± 0.025	0.934CysArg: 0.934 ± 0.034	0.661CysSer: 0.661 ± 0.033	0.586CysThr: 0.586 ± 0.031	0.663CysVal: 0.663 ± 0.029	0.182CysTrp: 0.182 ± 0.012	0.409CysTyr: 0.409 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	2.917AspAla: 2.917 ± 0.055	0.467AspCys: 0.467 ± 0.02	1.276AspAsp: 1.276 ± 0.042	2.618AspGlu: 2.618 ± 0.054	1.796AspPhe: 1.796 ± 0.043	2.961AspGly: 2.961 ± 0.061	0.631AspHis: 0.631 ± 0.026	3.216AspIle: 3.216 ± 0.056	2.056AspLys: 2.056 ± 0.046	5.06AspLeu: 5.06 ± 0.084	0.922AspMet: 0.922 ± 0.033	1.136AspAsn: 1.136 ± 0.036	2.731AspPro: 2.731 ± 0.059	1.11AspGln: 1.11 ± 0.037	2.501AspArg: 2.501 ± 0.054	1.571AspSer: 1.571 ± 0.036	2.037AspThr: 2.037 ± 0.05	2.994AspVal: 2.994 ± 0.063	0.54AspTrp: 0.54 ± 0.024	1.478AspTyr: 1.478 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	8.106GluAla: 8.106 ± 0.1	0.565GluCys: 0.565 ± 0.028	3.05GluAsp: 3.05 ± 0.063	8.23GluGlu: 8.23 ± 0.119	2.228GluPhe: 2.228 ± 0.043	6.035GluGly: 6.035 ± 0.098	1.15GluHis: 1.15 ± 0.037	5.611GluIle: 5.611 ± 0.083	5.107GluLys: 5.107 ± 0.075	8.258GluLeu: 8.258 ± 0.109	1.728GluMet: 1.728 ± 0.044	2.142GluAsn: 2.142 ± 0.046	2.493GluPro: 2.493 ± 0.055	2.376GluGln: 2.376 ± 0.061	5.26GluArg: 5.26 ± 0.085	2.176GluSer: 2.176 ± 0.045	2.918GluThr: 2.918 ± 0.064	6.879GluVal: 6.879 ± 0.097	0.799GluTrp: 0.799 ± 0.027	2.006GluTyr: 2.006 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	2.697PheAla: 2.697 ± 0.063	0.524PheCys: 0.524 ± 0.023	1.452PheAsp: 1.452 ± 0.042	1.702PheGlu: 1.702 ± 0.042	1.647PhePhe: 1.647 ± 0.047	2.792PheGly: 2.792 ± 0.06	0.697PheHis: 0.697 ± 0.028	2.401PheIle: 2.401 ± 0.054	1.685PheLys: 1.685 ± 0.045	4.931PheLeu: 4.931 ± 0.084	0.777PheMet: 0.777 ± 0.031	1.215PheAsn: 1.215 ± 0.036	1.975PhePro: 1.975 ± 0.051	1.087PheGln: 1.087 ± 0.034	2.254PheArg: 2.254 ± 0.049	2.18PheSer: 2.18 ± 0.06	2.086PheThr: 2.086 ± 0.046	2.211PheVal: 2.211 ± 0.053	0.577PheTrp: 0.577 ± 0.026	1.403PheTyr: 1.403 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	6.857GlyAla: 6.857 ± 0.1	1.212GlyCys: 1.212 ± 0.041	3.163GlyAsp: 3.163 ± 0.054	5.945GlyGlu: 5.945 ± 0.084	3.203GlyPhe: 3.203 ± 0.061	6.401GlyGly: 6.401 ± 0.112	1.491GlyHis: 1.491 ± 0.041	5.74GlyIle: 5.74 ± 0.075	4.695GlyLys: 4.695 ± 0.066	9.503GlyLeu: 9.503 ± 0.125	1.928GlyMet: 1.928 ± 0.045	2.316GlyAsn: 2.316 ± 0.057	3.208GlyPro: 3.208 ± 0.055	2.979GlyGln: 2.979 ± 0.057	5.614GlyArg: 5.614 ± 0.091	4.078GlySer: 4.078 ± 0.062	4.036GlyThr: 4.036 ± 0.063	6.409GlyVal: 6.409 ± 0.088	1.229GlyTrp: 1.229 ± 0.038	2.904GlyTyr: 2.904 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.172HisAla: 1.172 ± 0.035	0.262HisCys: 0.262 ± 0.019	0.619HisAsp: 0.619 ± 0.029	0.911HisGlu: 0.911 ± 0.032	0.765HisPhe: 0.765 ± 0.029	1.36HisGly: 1.36 ± 0.041	0.463HisHis: 0.463 ± 0.022	1.047HisIle: 1.047 ± 0.034	0.722HisLys: 0.722 ± 0.027	2.264HisLeu: 2.264 ± 0.05	0.316HisMet: 0.316 ± 0.02	0.536HisAsn: 0.536 ± 0.025	1.28HisPro: 1.28 ± 0.041	0.624HisGln: 0.624 ± 0.028	1.206HisArg: 1.206 ± 0.04	0.796HisSer: 0.796 ± 0.026	0.811HisThr: 0.811 ± 0.03	1.07HisVal: 1.07 ± 0.034	0.224HisTrp: 0.224 ± 0.016	0.601HisTyr: 0.601 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.722IleAla: 5.722 ± 0.082	0.704IleCys: 0.704 ± 0.029	2.692IleAsp: 2.692 ± 0.049	3.894IleGlu: 3.894 ± 0.069	2.378IlePhe: 2.378 ± 0.054	4.577IleGly: 4.577 ± 0.06	1.033IleHis: 1.033 ± 0.036	4.183IleIle: 4.183 ± 0.072	3.4IleLys: 3.4 ± 0.069	7.277IleLeu: 7.277 ± 0.104	1.224IleMet: 1.224 ± 0.04	2.051IleAsn: 2.051 ± 0.048	3.41IlePro: 3.41 ± 0.052	1.75IleGln: 1.75 ± 0.043	3.554IleArg: 3.554 ± 0.054	3.391IleSer: 3.391 ± 0.062	3.453IleThr: 3.453 ± 0.058	4.254IleVal: 4.254 ± 0.073	0.666IleTrp: 0.666 ± 0.03	1.842IleTyr: 1.842 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.988LysAla: 4.988 ± 0.078	0.427LysCys: 0.427 ± 0.023	2.294LysAsp: 2.294 ± 0.046	5.591LysGlu: 5.591 ± 0.087	1.471LysPhe: 1.471 ± 0.038	4.245LysGly: 4.245 ± 0.068	0.728LysHis: 0.728 ± 0.026	3.446LysIle: 3.446 ± 0.064	3.154LysLys: 3.154 ± 0.061	4.956LysLeu: 4.956 ± 0.065	1.197LysMet: 1.197 ± 0.031	1.438LysAsn: 1.438 ± 0.035	2.269LysPro: 2.269 ± 0.044	1.464LysGln: 1.464 ± 0.04	3.057LysArg: 3.057 ± 0.053	1.924LysSer: 1.924 ± 0.052	2.176LysThr: 2.176 ± 0.052	4.898LysVal: 4.898 ± 0.092	0.512LysTrp: 0.512 ± 0.024	1.489LysTyr: 1.489 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	12.663LeuAla: 12.663 ± 0.142	1.114LeuCys: 1.114 ± 0.037	4.816LeuAsp: 4.816 ± 0.075	9.416LeuGlu: 9.416 ± 0.118	3.788LeuPhe: 3.788 ± 0.084	9.586LeuGly: 9.586 ± 0.117	1.805LeuHis: 1.805 ± 0.05	6.032LeuIle: 6.032 ± 0.101	7.674LeuLys: 7.674 ± 0.092	12.358LeuLeu: 12.358 ± 0.174	2.135LeuMet: 2.135 ± 0.048	3.262LeuAsn: 3.262 ± 0.057	6.142LeuPro: 6.142 ± 0.085	3.363LeuGln: 3.363 ± 0.063	7.001LeuArg: 7.001 ± 0.11	6.177LeuSer: 6.177 ± 0.102	5.541LeuThr: 5.541 ± 0.067	8.691LeuVal: 8.691 ± 0.117	1.336LeuTrp: 1.336 ± 0.041	3.167LeuTyr: 3.167 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.384MetAla: 2.384 ± 0.055	0.2MetCys: 0.2 ± 0.014	0.898MetAsp: 0.898 ± 0.033	1.633MetGlu: 1.633 ± 0.037	0.607MetPhe: 0.607 ± 0.029	1.849MetGly: 1.849 ± 0.05	0.314MetHis: 0.314 ± 0.019	0.985MetIle: 0.985 ± 0.031	1.003MetLys: 1.003 ± 0.03	2.147MetLeu: 2.147 ± 0.042	0.394MetMet: 0.394 ± 0.022	0.551MetAsn: 0.551 ± 0.023	0.949MetPro: 0.949 ± 0.033	0.602MetGln: 0.602 ± 0.024	1.299MetArg: 1.299 ± 0.037	1.042MetSer: 1.042 ± 0.032	0.901MetThr: 0.901 ± 0.03	1.906MetVal: 1.906 ± 0.044	0.17MetTrp: 0.17 ± 0.014	0.453MetTyr: 0.453 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.024AsnAla: 2.024 ± 0.046	0.396AsnCys: 0.396 ± 0.021	0.886AsnAsp: 0.886 ± 0.035	1.425AsnGlu: 1.425 ± 0.044	1.216AsnPhe: 1.216 ± 0.038	1.919AsnGly: 1.919 ± 0.048	0.474AsnHis: 0.474 ± 0.022	2.111AsnIle: 2.111 ± 0.052	1.277AsnLys: 1.277 ± 0.036	3.866AsnLeu: 3.866 ± 0.068	0.612AsnMet: 0.612 ± 0.021	0.87AsnAsn: 0.87 ± 0.037	2.059AsnPro: 2.059 ± 0.05	0.798AsnGln: 0.798 ± 0.033	1.718AsnArg: 1.718 ± 0.046	1.298AsnSer: 1.298 ± 0.041	1.475AsnThr: 1.475 ± 0.041	1.907AsnVal: 1.907 ± 0.051	0.412AsnTrp: 0.412 ± 0.02	0.93AsnTyr: 0.93 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	4.043ProAla: 4.043 ± 0.072	0.574ProCys: 0.574 ± 0.025	2.319ProAsp: 2.319 ± 0.059	4.697ProGlu: 4.697 ± 0.068	1.853ProPhe: 1.853 ± 0.05	4.963ProGly: 4.963 ± 0.078	0.971ProHis: 0.971 ± 0.032	2.082ProIle: 2.082 ± 0.056	1.781ProLys: 1.781 ± 0.04	5.438ProLeu: 5.438 ± 0.093	0.726ProMet: 0.726 ± 0.026	1.139ProAsn: 1.139 ± 0.033	2.656ProPro: 2.656 ± 0.061	1.928ProGln: 1.928 ± 0.054	2.906ProArg: 2.906 ± 0.056	2.494ProSer: 2.494 ± 0.055	2.077ProThr: 2.077 ± 0.048	4.314ProVal: 4.314 ± 0.061	0.806ProTrp: 0.806 ± 0.031	1.759ProTyr: 1.759 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	3.822GlnAla: 3.822 ± 0.067	0.299GlnCys: 0.299 ± 0.018	1.44GlnAsp: 1.44 ± 0.039	3.615GlnGlu: 3.615 ± 0.083	0.828GlnPhe: 0.828 ± 0.035	3.204GlnGly: 3.204 ± 0.062	0.561GlnHis: 0.561 ± 0.025	1.776GlnIle: 1.776 ± 0.051	1.92GlnLys: 1.92 ± 0.044	2.812GlnLeu: 2.812 ± 0.063	0.639GlnMet: 0.639 ± 0.029	0.846GlnAsn: 0.846 ± 0.03	1.481GlnPro: 1.481 ± 0.047	1.205GlnGln: 1.205 ± 0.042	2.102GlnArg: 2.102 ± 0.054	1.18GlnSer: 1.18 ± 0.035	1.064GlnThr: 1.064 ± 0.037	3.354GlnVal: 3.354 ± 0.059	0.36GlnTrp: 0.36 ± 0.021	0.823GlnTyr: 0.823 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	5.337ArgAla: 5.337 ± 0.092	0.832ArgCys: 0.832 ± 0.032	2.615ArgAsp: 2.615 ± 0.057	6.206ArgGlu: 6.206 ± 0.086	2.217ArgPhe: 2.217 ± 0.049	4.709ArgGly: 4.709 ± 0.067	1.07ArgHis: 1.07 ± 0.033	3.8ArgIle: 3.8 ± 0.071	3.209ArgLys: 3.209 ± 0.063	7.841ArgLeu: 7.841 ± 0.103	1.381ArgMet: 1.381 ± 0.038	1.627ArgAsn: 1.627 ± 0.043	3.053ArgPro: 3.053 ± 0.053	2.835ArgGln: 2.835 ± 0.053	4.955ArgArg: 4.955 ± 0.09	2.677ArgSer: 2.677 ± 0.054	2.531ArgThr: 2.531 ± 0.049	5.353ArgVal: 5.353 ± 0.077	0.912ArgTrp: 0.912 ± 0.033	2.271ArgTyr: 2.271 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.431SerAla: 3.431 ± 0.066	0.662SerCys: 0.662 ± 0.025	1.596SerAsp: 1.596 ± 0.044	2.591SerGlu: 2.591 ± 0.047	2.3SerPhe: 2.3 ± 0.055	4.247SerGly: 4.247 ± 0.066	0.901SerHis: 0.901 ± 0.031	2.693SerIle: 2.693 ± 0.053	1.884SerLys: 1.884 ± 0.045	6.655SerLeu: 6.655 ± 0.096	0.974SerMet: 0.974 ± 0.032	1.277SerAsn: 1.277 ± 0.042	2.842SerPro: 2.842 ± 0.052	1.815SerGln: 1.815 ± 0.046	3.378SerArg: 3.378 ± 0.064	2.92SerSer: 2.92 ± 0.067	2.309SerThr: 2.309 ± 0.049	2.872SerVal: 2.872 ± 0.054	0.791SerTrp: 0.791 ± 0.039	1.528SerTyr: 1.528 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.173ThrAla: 4.173 ± 0.073	0.574ThrCys: 0.574 ± 0.025	1.675ThrAsp: 1.675 ± 0.039	2.56ThrGlu: 2.56 ± 0.049	1.884ThrPhe: 1.884 ± 0.044	4.779ThrGly: 4.779 ± 0.078	0.819ThrHis: 0.819 ± 0.025	2.764ThrIle: 2.764 ± 0.054	1.784ThrLys: 1.784 ± 0.037	5.673ThrLeu: 5.673 ± 0.082	0.851ThrMet: 0.851 ± 0.03	1.269ThrAsn: 1.269 ± 0.039	2.817ThrPro: 2.817 ± 0.056	1.365ThrGln: 1.365 ± 0.038	2.832ThrArg: 2.832 ± 0.057	2.633ThrSer: 2.633 ± 0.055	2.366ThrThr: 2.366 ± 0.052	3.763ThrVal: 3.763 ± 0.06	0.632ThrTrp: 0.632 ± 0.026	1.399ThrTyr: 1.399 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	8.036ValAla: 8.036 ± 0.095	0.888ValCys: 0.888 ± 0.032	3.717ValAsp: 3.717 ± 0.066	6.124ValGlu: 6.124 ± 0.087	2.719ValPhe: 2.719 ± 0.058	5.835ValGly: 5.835 ± 0.082	1.234ValHis: 1.234 ± 0.033	5.022ValIle: 5.022 ± 0.081	4.195ValLys: 4.195 ± 0.07	8.613ValLeu: 8.613 ± 0.11	1.541ValMet: 1.541 ± 0.039	2.308ValAsn: 2.308 ± 0.047	3.772ValPro: 3.772 ± 0.07	2.217ValGln: 2.217 ± 0.048	4.662ValArg: 4.662 ± 0.073	3.801ValSer: 3.801 ± 0.067	4.084ValThr: 4.084 ± 0.075	6.786ValVal: 6.786 ± 0.1	0.853ValTrp: 0.853 ± 0.033	2.275ValTyr: 2.275 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	1.091TrpAla: 1.091 ± 0.041	0.142TrpCys: 0.142 ± 0.012	0.551TrpAsp: 0.551 ± 0.024	1.268TrpGlu: 1.268 ± 0.037	0.358TrpPhe: 0.358 ± 0.017	1.157TrpGly: 1.157 ± 0.035	0.222TrpHis: 0.222 ± 0.015	0.583TrpIle: 0.583 ± 0.028	0.581TrpLys: 0.581 ± 0.026	1.433TrpLeu: 1.433 ± 0.044	0.281TrpMet: 0.281 ± 0.018	0.338TrpAsn: 0.338 ± 0.021	0.636TrpPro: 0.636 ± 0.028	0.66TrpGln: 0.66 ± 0.026	0.965TrpArg: 0.965 ± 0.036	0.573TrpSer: 0.573 ± 0.028	0.457TrpThr: 0.457 ± 0.025	0.974TrpVal: 0.974 ± 0.036	0.244TrpTrp: 0.244 ± 0.016	0.37TrpTyr: 0.37 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.339TyrAla: 2.339 ± 0.047	0.433TyrCys: 0.433 ± 0.022	1.255TyrAsp: 1.255 ± 0.039	1.791TyrGlu: 1.791 ± 0.04	1.433TyrPhe: 1.433 ± 0.038	2.656TyrGly: 2.656 ± 0.053	0.669TyrHis: 0.669 ± 0.029	1.825TyrIle: 1.825 ± 0.049	1.114TyrLys: 1.114 ± 0.035	3.929TyrLeu: 3.929 ± 0.067	0.497TyrMet: 0.497 ± 0.022	1.0TyrAsn: 1.0 ± 0.031	1.682TyrPro: 1.682 ± 0.044	1.302TyrGln: 1.302 ± 0.038	2.393TyrArg: 2.393 ± 0.052	1.475TyrSer: 1.475 ± 0.038	1.589TyrThr: 1.589 ± 0.045	1.937TyrVal: 1.937 ± 0.051	0.423TyrTrp: 0.423 ± 0.021	1.193TyrTyr: 1.193 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3229 proteins (972887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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