Tuber aestivum virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Totivirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5KIN7|E5KIN7_9VIRU RNA-directed RNA polymerase OS=Tuber aestivum virus 1 OX=927810 PE=3 SV=1
MM1 pKa = 7.3YY2 pKa = 8.49KK3 pKa = 9.5TFNSLSEE10 pKa = 4.04NAPVSTFEE18 pKa = 4.51TIQSRR23 pKa = 11.84GTTSLMTRR31 pKa = 11.84LRR33 pKa = 11.84LNLAYY38 pKa = 10.56DD39 pKa = 3.55NLDD42 pKa = 3.41FNRR45 pKa = 11.84VYY47 pKa = 10.77SSKK50 pKa = 10.62QDD52 pKa = 3.58YY53 pKa = 8.42TWLGKK58 pKa = 9.73AAAFIVEE65 pKa = 4.79AEE67 pKa = 4.26LSLDD71 pKa = 4.64GIAKK75 pKa = 10.04QYY77 pKa = 8.86LTLDD81 pKa = 3.36GAINFDD87 pKa = 3.49NVLAEE92 pKa = 4.14LRR94 pKa = 11.84TGAGLQTTNVSNHH107 pKa = 5.19SVSVSSWRR115 pKa = 11.84WYY117 pKa = 10.13DD118 pKa = 3.23NHH120 pKa = 5.66VTLLFNLLRR129 pKa = 11.84LYY131 pKa = 11.41VMADD135 pKa = 3.61LQLKK139 pKa = 10.29GPITTGSYY147 pKa = 9.95PKK149 pKa = 10.47YY150 pKa = 10.29DD151 pKa = 4.33DD152 pKa = 3.4GHH154 pKa = 7.98VEE156 pKa = 3.63IDD158 pKa = 3.99LNAGLPLPDD167 pKa = 3.93EE168 pKa = 4.79TIVWSWPGSKK178 pKa = 10.43NSLNYY183 pKa = 9.69PEE185 pKa = 4.64WGNFTEE191 pKa = 4.88YY192 pKa = 10.38MPLNDD197 pKa = 3.72EE198 pKa = 4.75AYY200 pKa = 9.54IDD202 pKa = 3.84VGGLLPEE209 pKa = 4.08EE210 pKa = 4.29VKK212 pKa = 10.18MVLMATGAWEE222 pKa = 3.8RR223 pKa = 11.84QTNFKK228 pKa = 10.9LDD230 pKa = 3.57FPTPKK235 pKa = 9.88LAEE238 pKa = 4.55RR239 pKa = 11.84IYY241 pKa = 10.78YY242 pKa = 9.83RR243 pKa = 11.84YY244 pKa = 8.63PSKK247 pKa = 10.6VTMIDD252 pKa = 3.04DD253 pKa = 3.81WLDD256 pKa = 3.56GEE258 pKa = 4.77IPDD261 pKa = 5.38INFGLPNPKK270 pKa = 9.78VVWNAIRR277 pKa = 11.84KK278 pKa = 8.49YY279 pKa = 9.01VTTNNLYY286 pKa = 9.75NQFYY290 pKa = 10.21SAAAIINQVMLTPLPDD306 pKa = 3.55SAEE309 pKa = 3.91GMSWLIHH316 pKa = 5.33EE317 pKa = 4.83AQVVLPKK324 pKa = 10.42FGSVRR329 pKa = 11.84GRR331 pKa = 11.84YY332 pKa = 8.49PFLLRR337 pKa = 11.84GEE339 pKa = 4.45GALIQAKK346 pKa = 10.16ALEE349 pKa = 4.83DD350 pKa = 3.14WTMLQTNPNLLFSTSMVTASLFNIGLAVRR379 pKa = 11.84DD380 pKa = 3.67SMLRR384 pKa = 11.84GIANDD389 pKa = 3.58SLSGVDD395 pKa = 3.37TSLFQQPEE403 pKa = 4.44LAWAAAVSLACGIDD417 pKa = 3.47VPLNGMSNCYY427 pKa = 10.04VYY429 pKa = 11.15YY430 pKa = 8.46PTLADD435 pKa = 3.68SNVRR439 pKa = 11.84LALPALIKK447 pKa = 9.86TPRR450 pKa = 11.84GYY452 pKa = 9.89WIEE455 pKa = 4.11GDD457 pKa = 3.96SIVIDD462 pKa = 3.55GVPFVGSPYY471 pKa = 10.62VLYY474 pKa = 10.02PLAVYY479 pKa = 10.45DD480 pKa = 4.11FANPYY485 pKa = 10.02SGAFALPKK493 pKa = 9.79PIRR496 pKa = 11.84RR497 pKa = 11.84TRR499 pKa = 11.84KK500 pKa = 7.97GAVFTVIGAWMYY512 pKa = 10.84SWVARR517 pKa = 11.84LAGYY521 pKa = 9.44DD522 pKa = 3.15VHH524 pKa = 9.03ISVNGSNVDD533 pKa = 3.42YY534 pKa = 11.1YY535 pKa = 11.46KK536 pKa = 11.17YY537 pKa = 10.31FASNEE542 pKa = 4.06NSWVHH547 pKa = 5.6PLWNGISDD555 pKa = 4.03EE556 pKa = 4.24IEE558 pKa = 4.11GVFVHH563 pKa = 7.25AIVRR567 pKa = 11.84RR568 pKa = 11.84NKK570 pKa = 10.2HH571 pKa = 5.78FLDD574 pKa = 3.85IPDD577 pKa = 3.43YY578 pKa = 9.86TIPGNLTDD586 pKa = 3.43VSVSVKK592 pKa = 10.33ILDD595 pKa = 3.95RR596 pKa = 11.84YY597 pKa = 10.69VSDD600 pKa = 4.16SSLTTKK606 pKa = 10.21FRR608 pKa = 11.84ANYY611 pKa = 9.2NVGSVTVPKK620 pKa = 10.61SGIQVVTSGDD630 pKa = 3.31LRR632 pKa = 11.84KK633 pKa = 9.37FWGYY637 pKa = 7.85VRR639 pKa = 11.84RR640 pKa = 11.84SQPGLTMVGMAQPAIMPQPKK660 pKa = 9.05HH661 pKa = 5.55QLVEE665 pKa = 3.95IDD667 pKa = 3.88QEE669 pKa = 4.42PEE671 pKa = 3.84VTSEE675 pKa = 3.92MVNMIEE681 pKa = 4.33

Molecular weight: 76.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5KIN7|E5KIN7_9VIRU RNA-directed RNA polymerase OS=Tuber aestivum virus 1 OX=927810 PE=3 SV=1
MM1 pKa = 7.37CAVSRR6 pKa = 11.84HH7 pKa = 5.66SSTHH11 pKa = 4.91MLVDD15 pKa = 5.21VIPCSQLGLGAVYY28 pKa = 10.25YY29 pKa = 8.48HH30 pKa = 6.44TEE32 pKa = 3.81VAVRR36 pKa = 11.84VYY38 pKa = 11.17GEE40 pKa = 3.75ARR42 pKa = 11.84YY43 pKa = 9.81CRR45 pKa = 11.84LFRR48 pKa = 11.84LDD50 pKa = 5.23DD51 pKa = 3.77VYY53 pKa = 11.53GCYY56 pKa = 9.58MRR58 pKa = 11.84TDD60 pKa = 3.53AYY62 pKa = 9.19TPCLGPVTKK71 pKa = 10.26RR72 pKa = 11.84LMSQVYY78 pKa = 9.6SLVNSVCFNSCDD90 pKa = 3.25ISDD93 pKa = 4.13YY94 pKa = 11.52LSLTLGSTLQMPKK107 pKa = 9.65IEE109 pKa = 4.31WPVKK113 pKa = 8.65HH114 pKa = 6.62GIYY117 pKa = 10.04EE118 pKa = 4.09RR119 pKa = 11.84SKK121 pKa = 9.47ITATHH126 pKa = 5.88HH127 pKa = 4.55THH129 pKa = 7.23LRR131 pKa = 11.84PQEE134 pKa = 4.03LEE136 pKa = 3.96AVAEE140 pKa = 4.39GMGGRR145 pKa = 11.84TKK147 pKa = 10.45FVYY150 pKa = 9.95AVCLQRR156 pKa = 11.84LLSIGVVTEE165 pKa = 4.08AVYY168 pKa = 11.06SSFLAYY174 pKa = 9.81IIGVEE179 pKa = 4.17YY180 pKa = 11.08NFALLLLRR188 pKa = 11.84STTVWSGTRR197 pKa = 11.84GEE199 pKa = 4.15EE200 pKa = 4.04QLLSKK205 pKa = 10.73LKK207 pKa = 10.82SFASAIKK214 pKa = 9.87ASHH217 pKa = 5.18SAKK220 pKa = 10.21YY221 pKa = 10.01QPLTQLFEE229 pKa = 4.31LTVLLNRR236 pKa = 11.84GIGSVDD242 pKa = 2.76WAGEE246 pKa = 3.82KK247 pKa = 10.29EE248 pKa = 4.16NRR250 pKa = 11.84VNPNTVDD257 pKa = 4.32LDD259 pKa = 3.75PADD262 pKa = 3.97VYY264 pKa = 10.8KK265 pKa = 11.04SSMTIFRR272 pKa = 11.84KK273 pKa = 9.64ARR275 pKa = 11.84KK276 pKa = 9.21SGYY279 pKa = 9.44KK280 pKa = 8.41YY281 pKa = 10.94PKK283 pKa = 7.81MHH285 pKa = 6.63YY286 pKa = 10.35DD287 pKa = 3.15KK288 pKa = 10.75FIEE291 pKa = 5.14SRR293 pKa = 11.84WEE295 pKa = 3.96WIPGGSVHH303 pKa = 6.1SQYY306 pKa = 11.16VDD308 pKa = 3.41DD309 pKa = 6.16DD310 pKa = 4.35EE311 pKa = 5.43YY312 pKa = 10.73IYY314 pKa = 10.64PGLYY318 pKa = 8.89TRR320 pKa = 11.84NKK322 pKa = 9.6FITVNKK328 pKa = 8.8MPSHH332 pKa = 6.73HH333 pKa = 6.68LQLFLSSTPQIKK345 pKa = 9.9AWTSTKK351 pKa = 10.5YY352 pKa = 10.25EE353 pKa = 3.85WGKK356 pKa = 9.95QRR358 pKa = 11.84AIYY361 pKa = 8.59GTDD364 pKa = 3.24LRR366 pKa = 11.84STIITNFAMYY376 pKa = 9.99RR377 pKa = 11.84CEE379 pKa = 4.29EE380 pKa = 4.21VLLHH384 pKa = 6.45LFPVGDD390 pKa = 3.53QADD393 pKa = 3.74VEE395 pKa = 4.61KK396 pKa = 10.39VHH398 pKa = 6.81RR399 pKa = 11.84RR400 pKa = 11.84ISMMLDD406 pKa = 3.65GYY408 pKa = 11.41DD409 pKa = 4.04SFCFDD414 pKa = 3.66YY415 pKa = 11.4DD416 pKa = 4.03DD417 pKa = 5.69FNSQHH422 pKa = 6.44SLSSMQAVLLAYY434 pKa = 9.34MDD436 pKa = 4.26AFSDD440 pKa = 3.95VMSPEE445 pKa = 3.89QLQAMEE451 pKa = 4.12WVILSVADD459 pKa = 3.84MQALSPTDD467 pKa = 3.63NEE469 pKa = 4.48WYY471 pKa = 10.41RR472 pKa = 11.84LTGTLLSGWRR482 pKa = 11.84LTTFMNTVLNWVYY495 pKa = 10.39MDD497 pKa = 3.33VAGVFNIDD505 pKa = 3.41GVHH508 pKa = 6.93DD509 pKa = 4.24SVHH512 pKa = 6.52NGDD515 pKa = 4.81DD516 pKa = 3.66VMISISSIKK525 pKa = 10.15AANLVMNKK533 pKa = 6.24MHH535 pKa = 6.9KK536 pKa = 10.34VNARR540 pKa = 11.84AQPTKK545 pKa = 10.81CNVLSISEE553 pKa = 4.07FLRR556 pKa = 11.84IEE558 pKa = 4.29HH559 pKa = 6.23GMSGYY564 pKa = 10.74DD565 pKa = 3.52GLGAQYY571 pKa = 10.96LSRR574 pKa = 11.84SCATLVHH581 pKa = 6.22SRR583 pKa = 11.84VEE585 pKa = 4.22SKK587 pKa = 11.1EE588 pKa = 4.08PITATRR594 pKa = 11.84LVEE597 pKa = 4.44ADD599 pKa = 3.77LSRR602 pKa = 11.84LKK604 pKa = 10.54DD605 pKa = 3.61YY606 pKa = 10.94RR607 pKa = 11.84SRR609 pKa = 11.84CANVDD614 pKa = 3.47AVDD617 pKa = 6.02LIQQQLFCRR626 pKa = 11.84TAKK629 pKa = 10.44LFNIEE634 pKa = 4.52LGNIYY639 pKa = 10.03KK640 pKa = 10.06ISEE643 pKa = 3.97LHH645 pKa = 6.06RR646 pKa = 11.84VVGGCSDD653 pKa = 3.89RR654 pKa = 11.84RR655 pKa = 11.84WAPIEE660 pKa = 3.93QQVTTTSGKK669 pKa = 10.03YY670 pKa = 9.13EE671 pKa = 4.15IPHH674 pKa = 6.65EE675 pKa = 4.4IDD677 pKa = 5.73DD678 pKa = 4.07PSFWPGVNDD687 pKa = 3.38YY688 pKa = 11.57SAMLYY693 pKa = 10.31RR694 pKa = 11.84KK695 pKa = 9.64IGEE698 pKa = 4.14QVKK701 pKa = 9.84MSVIVDD707 pKa = 3.81AVSQGSRR714 pKa = 11.84LTIAKK719 pKa = 9.7SRR721 pKa = 11.84SVTLAVEE728 pKa = 4.5RR729 pKa = 11.84TANIKK734 pKa = 9.95AKK736 pKa = 8.27EE737 pKa = 4.15WEE739 pKa = 3.92RR740 pKa = 11.84ALYY743 pKa = 8.33RR744 pKa = 11.84TYY746 pKa = 10.79KK747 pKa = 10.48GSTVHH752 pKa = 6.48YY753 pKa = 8.73YY754 pKa = 11.51ANLAKK759 pKa = 10.15FLNVPPLFGIEE770 pKa = 4.13TRR772 pKa = 11.84GGASAISAALASSDD786 pKa = 3.15TMRR789 pKa = 11.84ALKK792 pKa = 10.69VLLL795 pKa = 4.21

Molecular weight: 89.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1476

681

795

738.0

83.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.182 ± 0.008

1.152 ± 0.52

5.488 ± 0.145

4.607 ± 0.211

3.388 ± 0.172

6.098 ± 0.309

2.168 ± 0.513

5.285 ± 0.09

4.336 ± 0.403

9.824 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.157

4.675 ± 0.904

4.404 ± 0.891

3.117 ± 0.198

5.081 ± 0.499

8.401 ± 0.553

5.962 ± 0.054

8.333 ± 0.111

2.168 ± 0.288

5.285 ± 0.001
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski