Amino acid dipepetide frequency for Absiella sp. AM09-45

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.007AlaAla: 4.007 ± 0.078
1.158AlaCys: 1.158 ± 0.033
3.289AlaAsp: 3.289 ± 0.049
3.035AlaGlu: 3.035 ± 0.053
3.184AlaPhe: 3.184 ± 0.045
4.088AlaGly: 4.088 ± 0.069
1.288AlaHis: 1.288 ± 0.031
5.778AlaIle: 5.778 ± 0.073
4.689AlaLys: 4.689 ± 0.075
6.765AlaLeu: 6.765 ± 0.079
2.457AlaMet: 2.457 ± 0.05
2.884AlaAsn: 2.884 ± 0.063
1.488AlaPro: 1.488 ± 0.039
2.309AlaGln: 2.309 ± 0.047
2.002AlaArg: 2.002 ± 0.042
3.789AlaSer: 3.789 ± 0.069
2.939AlaThr: 2.939 ± 0.065
4.031AlaVal: 4.031 ± 0.066
0.571AlaTrp: 0.571 ± 0.019
3.238AlaTyr: 3.238 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.031CysAla: 1.031 ± 0.031
0.281CysCys: 0.281 ± 0.018
1.022CysAsp: 1.022 ± 0.028
0.988CysGlu: 0.988 ± 0.032
0.841CysPhe: 0.841 ± 0.025
1.227CysGly: 1.227 ± 0.035
0.338CysHis: 0.338 ± 0.016
1.491CysIle: 1.491 ± 0.036
0.957CysLys: 0.957 ± 0.035
1.207CysLeu: 1.207 ± 0.03
0.567CysMet: 0.567 ± 0.023
0.619CysAsn: 0.619 ± 0.022
0.51CysPro: 0.51 ± 0.021
0.375CysGln: 0.375 ± 0.016
0.505CysArg: 0.505 ± 0.02
0.848CysSer: 0.848 ± 0.028
0.638CysThr: 0.638 ± 0.023
1.012CysVal: 1.012 ± 0.028
0.103CysTrp: 0.103 ± 0.009
0.638CysTyr: 0.638 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.854AspAla: 3.854 ± 0.063
0.818AspCys: 0.818 ± 0.027
3.745AspAsp: 3.745 ± 0.065
5.21AspGlu: 5.21 ± 0.062
3.017AspPhe: 3.017 ± 0.048
3.862AspGly: 3.862 ± 0.075
1.443AspHis: 1.443 ± 0.034
5.979AspIle: 5.979 ± 0.07
4.23AspLys: 4.23 ± 0.06
5.001AspLeu: 5.001 ± 0.063
2.199AspMet: 2.199 ± 0.037
2.329AspAsn: 2.329 ± 0.048
1.637AspPro: 1.637 ± 0.038
2.059AspGln: 2.059 ± 0.044
1.764AspArg: 1.764 ± 0.038
2.73AspSer: 2.73 ± 0.048
3.318AspThr: 3.318 ± 0.055
4.115AspVal: 4.115 ± 0.062
0.485AspTrp: 0.485 ± 0.021
2.965AspTyr: 2.965 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
4.603GluAla: 4.603 ± 0.075
0.82GluCys: 0.82 ± 0.028
4.317GluAsp: 4.317 ± 0.057
6.373GluGlu: 6.373 ± 0.08
2.395GluPhe: 2.395 ± 0.039
3.491GluGly: 3.491 ± 0.062
1.699GluHis: 1.699 ± 0.036
5.826GluIle: 5.826 ± 0.077
6.794GluLys: 6.794 ± 0.078
6.049GluLeu: 6.049 ± 0.08
2.383GluMet: 2.383 ± 0.045
4.433GluAsn: 4.433 ± 0.065
1.629GluPro: 1.629 ± 0.035
2.549GluGln: 2.549 ± 0.048
2.584GluArg: 2.584 ± 0.051
3.073GluSer: 3.073 ± 0.056
3.518GluThr: 3.518 ± 0.056
4.197GluVal: 4.197 ± 0.06
0.573GluTrp: 0.573 ± 0.021
2.814GluTyr: 2.814 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
2.806PheAla: 2.806 ± 0.056
0.657PheCys: 0.657 ± 0.024
3.073PheAsp: 3.073 ± 0.056
2.765PheGlu: 2.765 ± 0.04
2.12PhePhe: 2.12 ± 0.05
2.711PheGly: 2.711 ± 0.048
1.309PheHis: 1.309 ± 0.035
3.961PheIle: 3.961 ± 0.065
2.535PheLys: 2.535 ± 0.047
4.327PheLeu: 4.327 ± 0.069
1.565PheMet: 1.565 ± 0.038
1.788PheAsn: 1.788 ± 0.041
1.355PhePro: 1.355 ± 0.038
1.66PheGln: 1.66 ± 0.038
1.186PheArg: 1.186 ± 0.031
2.902PheSer: 2.902 ± 0.055
2.621PheThr: 2.621 ± 0.05
3.008PheVal: 3.008 ± 0.052
0.327PheTrp: 0.327 ± 0.015
2.125PheTyr: 2.125 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
3.714GlyAla: 3.714 ± 0.074
1.141GlyCys: 1.141 ± 0.04
2.938GlyAsp: 2.938 ± 0.054
3.366GlyGlu: 3.366 ± 0.059
3.047GlyPhe: 3.047 ± 0.058
3.731GlyGly: 3.731 ± 0.078
1.1GlyHis: 1.1 ± 0.031
5.849GlyIle: 5.849 ± 0.071
4.996GlyLys: 4.996 ± 0.07
4.913GlyLeu: 4.913 ± 0.065
2.26GlyMet: 2.26 ± 0.046
3.082GlyAsn: 3.082 ± 0.068
1.039GlyPro: 1.039 ± 0.036
1.411GlyGln: 1.411 ± 0.034
1.891GlyArg: 1.891 ± 0.047
3.235GlySer: 3.235 ± 0.063
3.039GlyThr: 3.039 ± 0.052
4.127GlyVal: 4.127 ± 0.069
0.634GlyTrp: 0.634 ± 0.028
3.257GlyTyr: 3.257 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.498HisAla: 1.498 ± 0.037
0.368HisCys: 0.368 ± 0.019
1.455HisAsp: 1.455 ± 0.033
1.543HisGlu: 1.543 ± 0.038
1.042HisPhe: 1.042 ± 0.032
1.434HisGly: 1.434 ± 0.039
0.84HisHis: 0.84 ± 0.028
2.092HisIle: 2.092 ± 0.039
1.429HisLys: 1.429 ± 0.035
1.981HisLeu: 1.981 ± 0.043
0.798HisMet: 0.798 ± 0.026
0.875HisAsn: 0.875 ± 0.027
0.997HisPro: 0.997 ± 0.033
1.001HisGln: 1.001 ± 0.029
0.81HisArg: 0.81 ± 0.029
1.065HisSer: 1.065 ± 0.031
1.227HisThr: 1.227 ± 0.033
1.555HisVal: 1.555 ± 0.036
0.15HisTrp: 0.15 ± 0.01
1.045HisTyr: 1.045 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.069IleAla: 6.069 ± 0.071
1.469IleCys: 1.469 ± 0.036
5.227IleAsp: 5.227 ± 0.071
5.772IleGlu: 5.772 ± 0.063
3.649IlePhe: 3.649 ± 0.076
5.014IleGly: 5.014 ± 0.073
2.085IleHis: 2.085 ± 0.042
6.787IleIle: 6.787 ± 0.089
6.04IleLys: 6.04 ± 0.083
8.188IleLeu: 8.188 ± 0.105
2.568IleMet: 2.568 ± 0.044
3.883IleAsn: 3.883 ± 0.07
3.1IlePro: 3.1 ± 0.055
3.272IleGln: 3.272 ± 0.057
2.996IleArg: 2.996 ± 0.054
5.653IleSer: 5.653 ± 0.066
4.731IleThr: 4.731 ± 0.067
5.485IleVal: 5.485 ± 0.076
0.588IleTrp: 0.588 ± 0.024
3.61IleTyr: 3.61 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
5.195LysAla: 5.195 ± 0.085
0.738LysCys: 0.738 ± 0.025
5.945LysAsp: 5.945 ± 0.064
8.094LysGlu: 8.094 ± 0.086
2.086LysPhe: 2.086 ± 0.042
4.167LysGly: 4.167 ± 0.059
1.615LysHis: 1.615 ± 0.037
5.571LysIle: 5.571 ± 0.073
7.31LysLys: 7.31 ± 0.094
5.887LysLeu: 5.887 ± 0.071
2.551LysMet: 2.551 ± 0.045
4.358LysAsn: 4.358 ± 0.065
2.063LysPro: 2.063 ± 0.043
3.721LysGln: 3.721 ± 0.07
3.246LysArg: 3.246 ± 0.048
3.691LysSer: 3.691 ± 0.055
4.159LysThr: 4.159 ± 0.061
4.421LysVal: 4.421 ± 0.061
0.556LysTrp: 0.556 ± 0.021
3.046LysTyr: 3.046 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
5.711LeuAla: 5.711 ± 0.07
1.789LeuCys: 1.789 ± 0.042
5.545LeuAsp: 5.545 ± 0.069
5.591LeuGlu: 5.591 ± 0.068
4.313LeuPhe: 4.313 ± 0.071
5.129LeuGly: 5.129 ± 0.074
2.232LeuHis: 2.232 ± 0.042
7.15LeuIle: 7.15 ± 0.089
7.406LeuLys: 7.406 ± 0.08
8.953LeuLeu: 8.953 ± 0.102
3.06LeuMet: 3.06 ± 0.056
4.298LeuAsn: 4.298 ± 0.069
3.284LeuPro: 3.284 ± 0.056
3.426LeuGln: 3.426 ± 0.058
3.087LeuArg: 3.087 ± 0.062
6.682LeuSer: 6.682 ± 0.089
4.456LeuThr: 4.456 ± 0.061
4.871LeuVal: 4.871 ± 0.065
0.775LeuTrp: 0.775 ± 0.023
4.038LeuTyr: 4.038 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.026MetAla: 2.026 ± 0.047
0.384MetCys: 0.384 ± 0.017
2.116MetAsp: 2.116 ± 0.041
2.658MetGlu: 2.658 ± 0.054
1.281MetPhe: 1.281 ± 0.032
1.855MetGly: 1.855 ± 0.042
0.707MetHis: 0.707 ± 0.029
3.231MetIle: 3.231 ± 0.059
3.617MetLys: 3.617 ± 0.056
3.107MetLeu: 3.107 ± 0.047
1.371MetMet: 1.371 ± 0.038
2.02MetAsn: 2.02 ± 0.038
1.121MetPro: 1.121 ± 0.026
1.272MetGln: 1.272 ± 0.034
1.199MetArg: 1.199 ± 0.031
1.918MetSer: 1.918 ± 0.043
1.35MetThr: 1.35 ± 0.032
1.947MetVal: 1.947 ± 0.036
0.199MetTrp: 0.199 ± 0.012
1.104MetTyr: 1.104 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.214AsnAla: 3.214 ± 0.061
0.616AsnCys: 0.616 ± 0.022
3.195AsnAsp: 3.195 ± 0.059
3.772AsnGlu: 3.772 ± 0.064
1.866AsnPhe: 1.866 ± 0.039
3.32AsnGly: 3.32 ± 0.07
1.194AsnHis: 1.194 ± 0.031
4.171AsnIle: 4.171 ± 0.064
3.576AsnLys: 3.576 ± 0.068
3.918AsnLeu: 3.918 ± 0.06
1.609AsnMet: 1.609 ± 0.037
2.266AsnAsn: 2.266 ± 0.058
1.73AsnPro: 1.73 ± 0.041
1.953AsnGln: 1.953 ± 0.047
1.841AsnArg: 1.841 ± 0.037
2.252AsnSer: 2.252 ± 0.058
2.445AsnThr: 2.445 ± 0.057
3.113AsnVal: 3.113 ± 0.062
0.454AsnTrp: 0.454 ± 0.019
2.182AsnTyr: 2.182 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
1.719ProAla: 1.719 ± 0.04
0.479ProCys: 0.479 ± 0.022
1.85ProAsp: 1.85 ± 0.039
2.068ProGlu: 2.068 ± 0.047
1.588ProPhe: 1.588 ± 0.044
1.416ProGly: 1.416 ± 0.032
0.633ProHis: 0.633 ± 0.025
2.508ProIle: 2.508 ± 0.045
2.042ProLys: 2.042 ± 0.042
2.758ProLeu: 2.758 ± 0.056
0.974ProMet: 0.974 ± 0.028
1.471ProAsn: 1.471 ± 0.034
0.501ProPro: 0.501 ± 0.02
1.052ProGln: 1.052 ± 0.033
0.764ProArg: 0.764 ± 0.029
1.652ProSer: 1.652 ± 0.039
1.502ProThr: 1.502 ± 0.038
2.347ProVal: 2.347 ± 0.05
0.314ProTrp: 0.314 ± 0.016
1.621ProTyr: 1.621 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.34GlnAla: 2.34 ± 0.046
0.439GlnCys: 0.439 ± 0.018
2.093GlnAsp: 2.093 ± 0.043
2.641GlnGlu: 2.641 ± 0.054
1.366GlnPhe: 1.366 ± 0.034
1.965GlnGly: 1.965 ± 0.043
0.889GlnHis: 0.889 ± 0.029
3.084GlnIle: 3.084 ± 0.054
3.303GlnLys: 3.303 ± 0.056
3.577GlnLeu: 3.577 ± 0.057
1.355GlnMet: 1.355 ± 0.036
1.873GlnAsn: 1.873 ± 0.04
0.98GlnPro: 0.98 ± 0.028
1.591GlnGln: 1.591 ± 0.038
1.506GlnArg: 1.506 ± 0.039
2.006GlnSer: 2.006 ± 0.043
1.673GlnThr: 1.673 ± 0.038
2.117GlnVal: 2.117 ± 0.044
0.276GlnTrp: 0.276 ± 0.014
1.733GlnTyr: 1.733 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
1.683ArgAla: 1.683 ± 0.036
0.514ArgCys: 0.514 ± 0.02
1.698ArgAsp: 1.698 ± 0.042
2.333ArgGlu: 2.333 ± 0.049
1.76ArgPhe: 1.76 ± 0.038
1.637ArgGly: 1.637 ± 0.037
0.69ArgHis: 0.69 ± 0.028
3.253ArgIle: 3.253 ± 0.052
3.263ArgLys: 3.263 ± 0.055
3.199ArgLeu: 3.199 ± 0.055
1.323ArgMet: 1.323 ± 0.033
1.99ArgAsn: 1.99 ± 0.043
0.83ArgPro: 0.83 ± 0.03
1.226ArgGln: 1.226 ± 0.035
1.313ArgArg: 1.313 ± 0.039
1.765ArgSer: 1.765 ± 0.043
1.512ArgThr: 1.512 ± 0.04
1.971ArgVal: 1.971 ± 0.04
0.286ArgTrp: 0.286 ± 0.015
1.934ArgTyr: 1.934 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
3.36SerAla: 3.36 ± 0.059
0.834SerCys: 0.834 ± 0.026
3.426SerAsp: 3.426 ± 0.057
3.539SerGlu: 3.539 ± 0.044
3.186SerPhe: 3.186 ± 0.049
3.738SerGly: 3.738 ± 0.063
1.162SerHis: 1.162 ± 0.031
5.073SerIle: 5.073 ± 0.059
4.203SerLys: 4.203 ± 0.058
5.309SerLeu: 5.309 ± 0.064
2.034SerMet: 2.034 ± 0.044
2.737SerAsn: 2.737 ± 0.056
1.313SerPro: 1.313 ± 0.032
1.85SerGln: 1.85 ± 0.047
1.76SerArg: 1.76 ± 0.04
3.49SerSer: 3.49 ± 0.06
2.903SerThr: 2.903 ± 0.054
3.674SerVal: 3.674 ± 0.052
0.499SerTrp: 0.499 ± 0.024
2.746SerTyr: 2.746 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
3.035ThrAla: 3.035 ± 0.058
0.748ThrCys: 0.748 ± 0.026
2.418ThrAsp: 2.418 ± 0.052
2.287ThrGlu: 2.287 ± 0.042
2.44ThrPhe: 2.44 ± 0.046
3.291ThrGly: 3.291 ± 0.091
1.141ThrHis: 1.141 ± 0.032
4.798ThrIle: 4.798 ± 0.067
3.749ThrLys: 3.749 ± 0.055
5.282ThrLeu: 5.282 ± 0.075
1.707ThrMet: 1.707 ± 0.036
2.39ThrAsn: 2.39 ± 0.045
2.012ThrPro: 2.012 ± 0.044
1.777ThrGln: 1.777 ± 0.04
1.703ThrArg: 1.703 ± 0.039
3.242ThrSer: 3.242 ± 0.059
2.867ThrThr: 2.867 ± 0.063
2.955ThrVal: 2.955 ± 0.064
0.469ThrTrp: 0.469 ± 0.023
2.629ThrTyr: 2.629 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
3.751ValAla: 3.751 ± 0.056
1.116ValCys: 1.116 ± 0.03
3.67ValAsp: 3.67 ± 0.055
4.254ValGlu: 4.254 ± 0.066
3.03ValPhe: 3.03 ± 0.056
3.324ValGly: 3.324 ± 0.058
1.163ValHis: 1.163 ± 0.034
5.351ValIle: 5.351 ± 0.072
4.928ValLys: 4.928 ± 0.065
6.178ValLeu: 6.178 ± 0.077
2.012ValMet: 2.012 ± 0.039
3.09ValAsn: 3.09 ± 0.056
1.84ValPro: 1.84 ± 0.044
1.711ValGln: 1.711 ± 0.036
1.927ValArg: 1.927 ± 0.043
4.248ValSer: 4.248 ± 0.061
3.162ValThr: 3.162 ± 0.061
4.04ValVal: 4.04 ± 0.06
0.539ValTrp: 0.539 ± 0.021
2.755ValTyr: 2.755 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.408TrpAla: 0.408 ± 0.02
0.158TrpCys: 0.158 ± 0.011
0.469TrpAsp: 0.469 ± 0.021
0.51TrpGlu: 0.51 ± 0.02
0.416TrpPhe: 0.416 ± 0.017
0.545TrpGly: 0.545 ± 0.024
0.191TrpHis: 0.191 ± 0.012
0.771TrpIle: 0.771 ± 0.026
0.73TrpLys: 0.73 ± 0.026
0.822TrpLeu: 0.822 ± 0.025
0.321TrpMet: 0.321 ± 0.017
0.524TrpAsn: 0.524 ± 0.023
0.166TrpPro: 0.166 ± 0.012
0.324TrpGln: 0.324 ± 0.017
0.245TrpArg: 0.245 ± 0.013
0.382TrpSer: 0.382 ± 0.019
0.38TrpThr: 0.38 ± 0.02
0.428TrpVal: 0.428 ± 0.02
0.089TrpTrp: 0.089 ± 0.008
0.408TrpTyr: 0.408 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.963TyrAla: 2.963 ± 0.05
0.674TyrCys: 0.674 ± 0.023
3.268TyrAsp: 3.268 ± 0.069
3.323TyrGlu: 3.323 ± 0.057
2.176TyrPhe: 2.176 ± 0.043
2.826TyrGly: 2.826 ± 0.051
1.487TyrHis: 1.487 ± 0.037
3.46TyrIle: 3.46 ± 0.059
2.627TyrLys: 2.627 ± 0.048
4.375TyrLeu: 4.375 ± 0.068
1.368TyrMet: 1.368 ± 0.037
1.773TyrAsn: 1.773 ± 0.037
1.664TyrPro: 1.664 ± 0.04
2.268TyrGln: 2.268 ± 0.056
1.85TyrArg: 1.85 ± 0.046
2.213TyrSer: 2.213 ± 0.042
2.498TyrThr: 2.498 ± 0.047
2.672TyrVal: 2.672 ± 0.051
0.402TyrTrp: 0.402 ± 0.019
2.305TyrTyr: 2.305 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4075 proteins (1244012 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski