Cellulophaga phage phi47:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1PXS0|M1PXS0_9CAUD Structural protein OS=Cellulophaga phage phi47:1 OX=756281 GN=CDPG_00063 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.03NYY4 pKa = 10.66LSILFLLSSFLVLSQNPTSFRR25 pKa = 11.84NGVIPGVKK33 pKa = 7.19TTAEE37 pKa = 3.75IQAIVSPKK45 pKa = 10.56LGTQIFNSTTEE56 pKa = 4.5TIWVRR61 pKa = 11.84QSSSWADD68 pKa = 3.22TGVGGTVTGDD78 pKa = 3.14VVTFDD83 pKa = 3.39SSKK86 pKa = 11.29ANAVDD91 pKa = 3.65VEE93 pKa = 4.53IVDD96 pKa = 4.24GDD98 pKa = 3.81PSVDD102 pKa = 3.35YY103 pKa = 11.1KK104 pKa = 11.56LLFDD108 pKa = 5.0INGTGWGAEE117 pKa = 4.44TPTSTWGQIQGLLSDD132 pKa = 3.85QTDD135 pKa = 3.32LATALNNKK143 pKa = 9.38ISFSGANLGTSTFILGGSGNNIIAFSRR170 pKa = 11.84LDD172 pKa = 3.52NNTTYY177 pKa = 11.3VMSKK181 pKa = 10.16LKK183 pKa = 10.89HH184 pKa = 5.66EE185 pKa = 4.69FSLGNDD191 pKa = 3.72DD192 pKa = 5.8DD193 pKa = 5.27SVTSVYY199 pKa = 10.69TMEE202 pKa = 5.1PDD204 pKa = 4.2KK205 pKa = 10.85FTTDD209 pKa = 3.02LTLAEE214 pKa = 4.48VKK216 pKa = 10.66AGGSGQFLTRR226 pKa = 11.84EE227 pKa = 4.34AGDD230 pKa = 4.27DD231 pKa = 3.75LYY233 pKa = 11.6APAGSTGGSEE243 pKa = 4.33EE244 pKa = 4.06NTDD247 pKa = 3.8NQTLSYY253 pKa = 10.6NDD255 pKa = 3.56YY256 pKa = 11.39GEE258 pKa = 4.11ISITGGNTITATRR271 pKa = 11.84YY272 pKa = 10.08RR273 pKa = 11.84NITTFDD279 pKa = 4.78LIDD282 pKa = 4.72DD283 pKa = 4.72GGGATYY289 pKa = 10.67SPTAFGVSEE298 pKa = 4.27TYY300 pKa = 10.32EE301 pKa = 3.85FSDD304 pKa = 3.54NVLGAVYY311 pKa = 10.19VNPIVTGTPTGDD323 pKa = 3.21LVFDD327 pKa = 4.75LGASYY332 pKa = 10.85EE333 pKa = 4.26VGSTNWSIFDD343 pKa = 3.61VFISNVNNTGDD354 pKa = 3.8DD355 pKa = 4.24YY356 pKa = 11.57YY357 pKa = 11.72LKK359 pKa = 10.45AIKK362 pKa = 8.75RR363 pKa = 11.84TKK365 pKa = 10.35VSSPDD370 pKa = 3.86DD371 pKa = 3.31IYY373 pKa = 11.52KK374 pKa = 10.37IMSVDD379 pKa = 3.25ASGKK383 pKa = 9.61LVPFPAPLSFDD394 pKa = 3.94TINLKK399 pKa = 10.1LQYY402 pKa = 10.8NFYY405 pKa = 10.43PYY407 pKa = 11.3

Molecular weight: 43.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1Q6Z4|M1Q6Z4_9CAUD Uncharacterized protein OS=Cellulophaga phage phi47:1 OX=756281 GN=CDPG_00024 PE=4 SV=1
MM1 pKa = 6.98YY2 pKa = 10.26KK3 pKa = 10.28SRR5 pKa = 11.84IKK7 pKa = 10.22IQRR10 pKa = 11.84KK11 pKa = 5.85TSYY14 pKa = 11.49SNLEE18 pKa = 3.89PKK20 pKa = 10.09NRR22 pKa = 11.84SIMEE26 pKa = 4.31HH27 pKa = 6.25EE28 pKa = 4.31NQATRR33 pKa = 11.84AMRR36 pKa = 11.84NFSDD40 pKa = 3.6QDD42 pKa = 3.52SMNNSKK48 pKa = 10.96AKK50 pKa = 9.28TISGITAAKK59 pKa = 8.22TASKK63 pKa = 9.76WLRR66 pKa = 11.84NHH68 pKa = 6.39KK69 pKa = 8.22QTNNGKK75 pKa = 9.09NFAKK79 pKa = 10.75NEE81 pKa = 3.95NN82 pKa = 3.69

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

17824

51

1094

225.6

25.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.559 ± 0.389

0.814 ± 0.096

6.003 ± 0.198

7.058 ± 0.365

4.623 ± 0.199

6.048 ± 0.323

1.565 ± 0.122

6.777 ± 0.22

9.403 ± 0.649

8.259 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.009 ± 0.137

6.413 ± 0.243

2.8 ± 0.147

3.282 ± 0.138

3.944 ± 0.363

7.787 ± 0.302

5.846 ± 0.253

6.351 ± 0.227

1.133 ± 0.126

3.327 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski