Pothos latent virus (isolate Pigeonpea/India) (PoLV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Aureusvirus; Pothos latent virus

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9QBU1|P14_POLVP RNA silencing suppressor p14 OS=Pothos latent virus (isolate Pigeonpea/India) OX=652109 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.57ANWCMSVLMFSFVTVYY17 pKa = 10.34TIVTSLGQGIRR28 pKa = 11.84RR29 pKa = 11.84GFRR32 pKa = 11.84AIVDD36 pKa = 3.86EE37 pKa = 4.35VEE39 pKa = 4.32NIYY42 pKa = 11.39GDD44 pKa = 3.35MCAGFKK50 pKa = 10.41VALSKK55 pKa = 11.2LSVSWIVVVGMIFVLNSLVVGLVPTLTMVAIIAACVGAKK94 pKa = 10.1YY95 pKa = 10.28GGRR98 pKa = 11.84VPRR101 pKa = 11.84YY102 pKa = 8.99IRR104 pKa = 11.84AHH106 pKa = 5.44VDD108 pKa = 4.04RR109 pKa = 11.84IRR111 pKa = 11.84KK112 pKa = 8.57SWEE115 pKa = 3.68KK116 pKa = 10.95GVDD119 pKa = 4.39DD120 pKa = 5.8DD121 pKa = 5.63DD122 pKa = 4.72CCAPLPEE129 pKa = 4.5TSLEE133 pKa = 4.05RR134 pKa = 11.84VPTKK138 pKa = 10.41LRR140 pKa = 11.84PRR142 pKa = 11.84LACKK146 pKa = 9.74IAVRR150 pKa = 11.84AIAKK154 pKa = 9.99VGLLKK159 pKa = 10.63RR160 pKa = 11.84SEE162 pKa = 4.34ANSMVYY168 pKa = 9.97QRR170 pKa = 11.84VCLDD174 pKa = 3.14VMEE177 pKa = 4.42SMKK180 pKa = 10.07MRR182 pKa = 11.84WHH184 pKa = 6.99DD185 pKa = 3.63RR186 pKa = 11.84LVILPQAVLACLEE199 pKa = 4.01RR200 pKa = 11.84PEE202 pKa = 4.2EE203 pKa = 4.11VEE205 pKa = 4.1EE206 pKa = 4.07VMKK209 pKa = 10.92AIEE212 pKa = 4.32ASCKK216 pKa = 10.41GRR218 pKa = 11.84FDD220 pKa = 3.98VYY222 pKa = 10.07XGCLLQHH229 pKa = 6.83RR230 pKa = 11.84GYY232 pKa = 8.77NTAIISAIPDD242 pKa = 3.55GVLLNHH248 pKa = 6.85EE249 pKa = 4.32GMVVRR254 pKa = 11.84KK255 pKa = 8.52GAPITTEE262 pKa = 3.85RR263 pKa = 11.84KK264 pKa = 7.59WYY266 pKa = 10.57SFAGYY271 pKa = 10.4ASTYY275 pKa = 10.04EE276 pKa = 4.24YY277 pKa = 10.18IVHH280 pKa = 6.48NSSLVNVCRR289 pKa = 11.84GLVEE293 pKa = 4.85RR294 pKa = 11.84VFCVVRR300 pKa = 11.84DD301 pKa = 4.08GKK303 pKa = 9.68LQRR306 pKa = 11.84PLRR309 pKa = 11.84PKK311 pKa = 10.72NNVFEE316 pKa = 4.67RR317 pKa = 11.84KK318 pKa = 9.26LGNVGRR324 pKa = 11.84RR325 pKa = 11.84ISSIVGYY332 pKa = 9.29CPAMTRR338 pKa = 11.84SEE340 pKa = 4.15FCEE343 pKa = 4.4SYY345 pKa = 10.65SGTRR349 pKa = 11.84RR350 pKa = 11.84ATYY353 pKa = 9.75EE354 pKa = 3.85RR355 pKa = 11.84ARR357 pKa = 11.84LSLDD361 pKa = 3.18VLPCTRR367 pKa = 11.84KK368 pKa = 9.84DD369 pKa = 3.24AYY371 pKa = 11.22LKK373 pKa = 10.07TFVKK377 pKa = 10.54AEE379 pKa = 4.43KK380 pKa = 10.4INITLKK386 pKa = 10.25PDD388 pKa = 3.82PAPRR392 pKa = 11.84VIQPRR397 pKa = 11.84DD398 pKa = 3.05PRR400 pKa = 11.84YY401 pKa = 8.24NVEE404 pKa = 3.6VGRR407 pKa = 11.84YY408 pKa = 7.77LKK410 pKa = 10.36PLEE413 pKa = 4.31PRR415 pKa = 11.84LMKK418 pKa = 10.74AIDD421 pKa = 3.85KK422 pKa = 10.71LWGEE426 pKa = 3.96KK427 pKa = 8.64TAIKK431 pKa = 10.27GYY433 pKa = 6.52TVEE436 pKa = 4.31KK437 pKa = 10.33VGEE440 pKa = 4.02ILHH443 pKa = 5.5QKK445 pKa = 8.49SLRR448 pKa = 11.84FKK450 pKa = 9.78NPCFVGLDD458 pKa = 3.29ASRR461 pKa = 11.84FDD463 pKa = 3.64QHH465 pKa = 7.34CSRR468 pKa = 11.84QALEE472 pKa = 4.51WEE474 pKa = 4.17HH475 pKa = 7.25SVYY478 pKa = 10.83NAIFRR483 pKa = 11.84DD484 pKa = 4.23PYY486 pKa = 10.98LSEE489 pKa = 4.3LLTWQIDD496 pKa = 4.22NVGTAYY502 pKa = 10.93LKK504 pKa = 10.95DD505 pKa = 3.2GFVRR509 pKa = 11.84YY510 pKa = 9.28RR511 pKa = 11.84VDD513 pKa = 3.25GCRR516 pKa = 11.84MSGDD520 pKa = 3.85MNTSMGNYY528 pKa = 10.0LIMSCLVYY536 pKa = 9.28QFCKK540 pKa = 10.63DD541 pKa = 3.15IGIDD545 pKa = 4.0ASLANCGDD553 pKa = 3.62DD554 pKa = 3.56CVLYY558 pKa = 10.76LEE560 pKa = 5.81KK561 pKa = 10.79EE562 pKa = 4.2DD563 pKa = 4.5LPKK566 pKa = 10.98LKK568 pKa = 10.46ALPDD572 pKa = 3.37WFGKK576 pKa = 9.1MGYY579 pKa = 8.44SMKK582 pKa = 10.2VEE584 pKa = 3.95KK585 pKa = 10.24PVFTVEE591 pKa = 4.44EE592 pKa = 4.2IEE594 pKa = 4.63FCQQHH599 pKa = 6.18PVQLSRR605 pKa = 11.84GYY607 pKa = 11.23VMVRR611 pKa = 11.84RR612 pKa = 11.84PDD614 pKa = 3.14VCLTKK619 pKa = 10.46DD620 pKa = 3.3LCVVRR625 pKa = 11.84GGMTTEE631 pKa = 4.28RR632 pKa = 11.84LQRR635 pKa = 11.84WLYY638 pKa = 8.84AQHH641 pKa = 7.37DD642 pKa = 4.28GGLALTGDD650 pKa = 4.49CPVLGAFYY658 pKa = 10.71RR659 pKa = 11.84RR660 pKa = 11.84FPSGEE665 pKa = 3.88SHH667 pKa = 6.95GEE669 pKa = 3.5LSEE672 pKa = 4.15YY673 pKa = 11.13SDD675 pKa = 3.14AHH677 pKa = 6.14KK678 pKa = 10.65FKK680 pKa = 10.88AGKK683 pKa = 9.85QYY685 pKa = 11.72GEE687 pKa = 4.15INSLARR693 pKa = 11.84YY694 pKa = 8.5SFWLAFGITPDD705 pKa = 3.32EE706 pKa = 3.92QLAIEE711 pKa = 4.68RR712 pKa = 11.84DD713 pKa = 3.68LMNWTPNVVPGSCDD727 pKa = 3.37PRR729 pKa = 11.84PTLLDD734 pKa = 3.46YY735 pKa = 11.46CSDD738 pKa = 3.48NN739 pKa = 3.65

Molecular weight: 83.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9QBU3|CAPSD_POLVP Capsid protein OS=Pothos latent virus (isolate Pigeonpea/India) OX=652109 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.59ALVKK5 pKa = 10.53RR6 pKa = 11.84NNNMALIATEE16 pKa = 4.13AGKK19 pKa = 9.96LAAVKK24 pKa = 10.09AGQVMLSPAGRR35 pKa = 11.84EE36 pKa = 4.14LIWNGVNWVRR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 10.2LGRR51 pKa = 11.84SKK53 pKa = 10.83KK54 pKa = 10.15SDD56 pKa = 3.67VILHH60 pKa = 6.32PGVLPGAIAAPVANTRR76 pKa = 11.84IIRR79 pKa = 11.84ASKK82 pKa = 10.4PKK84 pKa = 8.67FTRR87 pKa = 11.84SKK89 pKa = 10.13GSVTIAHH96 pKa = 6.98RR97 pKa = 11.84EE98 pKa = 3.74LLGQFNNSSGLVVNGGVSGNLYY120 pKa = 10.34RR121 pKa = 11.84INPSNPVVFPWLQGIAASFDD141 pKa = 3.23QYY143 pKa = 11.72KK144 pKa = 9.98FDD146 pKa = 4.33RR147 pKa = 11.84VQLQYY152 pKa = 11.56VPMCATTEE160 pKa = 4.0TGRR163 pKa = 11.84VAIYY167 pKa = 10.17FDD169 pKa = 4.79KK170 pKa = 10.84DD171 pKa = 3.53QQDD174 pKa = 3.61VEE176 pKa = 4.15PADD179 pKa = 3.91RR180 pKa = 11.84VEE182 pKa = 4.15LANMGHH188 pKa = 5.31WTEE191 pKa = 4.04SAPWCEE197 pKa = 4.01STLNVPVDD205 pKa = 3.46NVKK208 pKa = 10.82RR209 pKa = 11.84FMNDD213 pKa = 2.15NTTTDD218 pKa = 3.26RR219 pKa = 11.84KK220 pKa = 10.46LVDD223 pKa = 4.24LGQIGLATYY232 pKa = 10.59GGGSTNPVGDD242 pKa = 4.1LFIHH246 pKa = 4.8YY247 pKa = 7.38TITLFEE253 pKa = 4.42PQPLASLVEE262 pKa = 4.45TEE264 pKa = 4.05QSGAGAAPFGTNLVTVSSNATTTIITFRR292 pKa = 11.84GPGVYY297 pKa = 10.49LLALSQRR304 pKa = 11.84AASFTTLVTAGGAVVNSHH322 pKa = 5.28TTLFSGPAYY331 pKa = 10.33QSIANITATIPGGSITYY348 pKa = 10.42NGTLFGNYY356 pKa = 7.23TLQVTRR362 pKa = 11.84AKK364 pKa = 10.24ISNNATLII372 pKa = 3.97

Molecular weight: 39.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1483

130

739

370.8

41.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.676 ± 1.122

2.495 ± 0.948

4.316 ± 0.657

4.99 ± 0.67

3.574 ± 0.166

8.159 ± 0.677

1.821 ± 0.335

4.922 ± 0.265

5.057 ± 0.675

8.699 ± 0.49

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.412

3.978 ± 0.934

4.99 ± 0.306

3.709 ± 0.922

6.541 ± 0.885

7.822 ± 1.782

5.934 ± 1.315

8.833 ± 0.502

1.416 ± 0.142

3.506 ± 0.582
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski