Alkaliphilus metalliredigens (strain QYMF)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Alkaliphilus; Alkaliphilus metalliredigens

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4467 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6TSE0|A6TSE0_ALKMQ [Citrate [pro-3S]-lyase] ligase OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826 GN=Amet_2958 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.12KK3 pKa = 10.71GLIITLLITSMLFMVGFAPTAGQSYY28 pKa = 7.83WEE30 pKa = 4.21EE31 pKa = 3.83MKK33 pKa = 10.55KK34 pKa = 10.48VYY36 pKa = 9.8EE37 pKa = 3.96WDD39 pKa = 3.36AMEE42 pKa = 5.58GDD44 pKa = 3.68TDD46 pKa = 4.5LEE48 pKa = 4.57VIISVPGQLEE58 pKa = 3.77ATYY61 pKa = 10.43NITMYY66 pKa = 10.81SQTDD70 pKa = 3.62LEE72 pKa = 4.69AFVSYY77 pKa = 10.91IEE79 pKa = 4.18MEE81 pKa = 4.3VEE83 pKa = 3.57IEE85 pKa = 4.03EE86 pKa = 4.48ADD88 pKa = 3.82EE89 pKa = 4.28TTHH92 pKa = 7.03QIPTVKK98 pKa = 9.83MYY100 pKa = 10.55TKK102 pKa = 10.69GSNIYY107 pKa = 10.2INTDD111 pKa = 3.52LVVHH115 pKa = 6.55LLSLAEE121 pKa = 4.11SEE123 pKa = 5.31LPFEE127 pKa = 4.59TEE129 pKa = 3.47AEE131 pKa = 4.29YY132 pKa = 10.95IMLQSGEE139 pKa = 4.5NNMNMDD145 pKa = 4.61LTSLTDD151 pKa = 3.76IIGFLEE157 pKa = 4.25NMDD160 pKa = 4.29LGIDD164 pKa = 3.69LGMTQEE170 pKa = 4.12GDD172 pKa = 3.59TYY174 pKa = 10.75SLRR177 pKa = 11.84IEE179 pKa = 4.11SDD181 pKa = 3.02KK182 pKa = 11.15MIDD185 pKa = 4.15LLDD188 pKa = 3.68VYY190 pKa = 10.45IKK192 pKa = 10.77YY193 pKa = 10.8VITNMDD199 pKa = 3.24QLTNLFMQGDD209 pKa = 4.13VPEE212 pKa = 4.37EE213 pKa = 3.79MVLTEE218 pKa = 3.96EE219 pKa = 4.38QQAEE223 pKa = 4.31MLEE226 pKa = 4.17MYY228 pKa = 10.38EE229 pKa = 4.94MYY231 pKa = 9.1ITPQKK236 pKa = 11.36DD237 pKa = 3.12MAKK240 pKa = 10.32GFILGSYY247 pKa = 8.53YY248 pKa = 10.88DD249 pKa = 3.58QVTTFHH255 pKa = 6.61EE256 pKa = 4.04NSYY259 pKa = 9.84TEE261 pKa = 4.13DD262 pKa = 3.54AEE264 pKa = 5.33LFFTTPMGKK273 pKa = 7.94MHH275 pKa = 7.44IDD277 pKa = 3.97MISSGQKK284 pKa = 10.24LDD286 pKa = 3.32TTTIEE291 pKa = 4.33LPTSVMVITEE301 pKa = 4.34DD302 pKa = 3.39EE303 pKa = 4.48LTNLMISGFGATQDD317 pKa = 3.64MPEE320 pKa = 4.35GSQLQAIVDD329 pKa = 4.03LQGDD333 pKa = 3.92YY334 pKa = 10.92MKK336 pKa = 9.62ITEE339 pKa = 4.2GEE341 pKa = 4.2VLEE344 pKa = 4.81GNINLKK350 pKa = 10.21VEE352 pKa = 4.29EE353 pKa = 4.42GRR355 pKa = 11.84SYY357 pKa = 11.04IVVEE361 pKa = 5.13DD362 pKa = 3.63IQEE365 pKa = 4.13LLDD368 pKa = 3.75IEE370 pKa = 4.71FEE372 pKa = 4.13GLEE375 pKa = 4.29GFMMIQDD382 pKa = 4.43LEE384 pKa = 4.33QYY386 pKa = 10.55GYY388 pKa = 10.44QIQWNPDD395 pKa = 2.61SRR397 pKa = 11.84TIEE400 pKa = 3.91IYY402 pKa = 10.7NN403 pKa = 3.43

Molecular weight: 46.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6TJG9|A6TJG9_ALKMQ Nicotinate phosphoribosyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826 GN=Amet_0094 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.47EE15 pKa = 3.72HH16 pKa = 6.09GFVKK20 pKa = 10.46RR21 pKa = 11.84MRR23 pKa = 11.84TTSGRR28 pKa = 11.84RR29 pKa = 11.84ILKK32 pKa = 9.77RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.28GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4467

0

4467

1321948

34

2843

295.9

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.331 ± 0.035

0.924 ± 0.014

5.189 ± 0.027

7.81 ± 0.044

4.125 ± 0.032

6.997 ± 0.04

1.893 ± 0.016

9.05 ± 0.034

7.314 ± 0.04

9.439 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.019 ± 0.018

4.82 ± 0.027

3.211 ± 0.022

3.491 ± 0.026

4.021 ± 0.023

5.803 ± 0.029

5.214 ± 0.029

6.893 ± 0.029

0.782 ± 0.012

3.676 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski