Noviherbaspirillum autotrophicum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Noviherbaspirillum

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2BMD9|A0A0C2BMD9_9BURK Phospholipid-binding protein OS=Noviherbaspirillum autotrophicum OX=709839 GN=TSA66_10745 PE=4 SV=1
MM1 pKa = 7.15NAVTEE6 pKa = 4.21MPEE9 pKa = 4.12PIIFTDD15 pKa = 3.62SAADD19 pKa = 3.56KK20 pKa = 10.16VAQLIEE26 pKa = 4.39EE27 pKa = 4.51EE28 pKa = 4.63GNPDD32 pKa = 2.85LKK34 pKa = 11.13LRR36 pKa = 11.84VFVQGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.69EE55 pKa = 4.93IVNEE59 pKa = 5.02DD60 pKa = 4.04DD61 pKa = 3.2TTMTKK66 pKa = 10.66NGVQLLIDD74 pKa = 3.77SMSYY78 pKa = 10.23QYY80 pKa = 11.55LVGAEE85 pKa = 4.01IDD87 pKa = 3.89YY88 pKa = 11.28KK89 pKa = 11.39DD90 pKa = 4.47DD91 pKa = 4.08LEE93 pKa = 4.56GAQFVIKK100 pKa = 10.48NPNASTTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1YRP6|A0A0C1YRP6_9BURK Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain protein OS=Noviherbaspirillum autotrophicum OX=709839 GN=TSA66_24955 PE=4 SV=1
MM1 pKa = 7.41LRR3 pKa = 11.84VLLALRR9 pKa = 11.84APPVPPTPPARR20 pKa = 11.84PTLLPVLPAPTRR32 pKa = 11.84AALVLPALIRR42 pKa = 11.84AAPARR47 pKa = 11.84RR48 pKa = 11.84ALLRR52 pKa = 11.84AAPVHH57 pKa = 5.44QALIRR62 pKa = 11.84AGPARR67 pKa = 11.84PHH69 pKa = 5.49RR70 pKa = 11.84AIRR73 pKa = 11.84ARR75 pKa = 11.84AHH77 pKa = 6.18RR78 pKa = 11.84AACRR82 pKa = 11.84VRR84 pKa = 11.84ADD86 pKa = 3.94RR87 pKa = 11.84RR88 pKa = 11.84PPAPAA93 pKa = 4.47

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4102

0

4102

1246646

37

3225

303.9

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.919 ± 0.053

1.01 ± 0.013

5.307 ± 0.025

5.543 ± 0.037

3.748 ± 0.027

7.732 ± 0.031

2.35 ± 0.019

5.181 ± 0.029

4.105 ± 0.033

10.38 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.02

3.066 ± 0.024

4.823 ± 0.026

3.986 ± 0.024

6.475 ± 0.036

5.527 ± 0.028

5.021 ± 0.028

7.308 ± 0.034

1.299 ± 0.016

2.53 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski