Pedobacter rhizosphaerae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9M7V8|A0A1H9M7V8_9SPHI UPF0502 protein SAMN04488023_105153 OS=Pedobacter rhizosphaerae OX=390241 GN=SAMN04488023_105153 PE=3 SV=1
MM1 pKa = 7.22VCDD4 pKa = 3.58TQTIFEE10 pKa = 5.34FIVCDD15 pKa = 3.63PDD17 pKa = 3.51ATNKK21 pKa = 9.98TIFEE25 pKa = 4.96FIVCDD30 pKa = 3.63PDD32 pKa = 3.51ATNKK36 pKa = 9.98TIFEE40 pKa = 4.96FIVCDD45 pKa = 3.63PDD47 pKa = 3.51ATNKK51 pKa = 9.98TIFEE55 pKa = 4.96FIVCDD60 pKa = 3.63PDD62 pKa = 3.51ATNKK66 pKa = 10.05TIFEE70 pKa = 4.49FKK72 pKa = 10.75ACDD75 pKa = 3.52PDD77 pKa = 3.49ATNN80 pKa = 4.04

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9T1M0|A0A1H9T1M0_9SPHI Uncharacterized protein OS=Pedobacter rhizosphaerae OX=390241 GN=SAMN04488023_12047 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84RR3 pKa = 11.84GWNRR7 pKa = 11.84FSGKK11 pKa = 10.48GNLRR15 pKa = 11.84EE16 pKa = 4.24SPLKK20 pKa = 10.71DD21 pKa = 3.16EE22 pKa = 5.33QINKK26 pKa = 9.83NYY28 pKa = 10.67NEE30 pKa = 3.92LSFRR34 pKa = 11.84LARR37 pKa = 11.84VILRR41 pKa = 11.84RR42 pKa = 11.84QSEE45 pKa = 3.98LAGYY49 pKa = 9.45FNAKK53 pKa = 9.37ASKK56 pKa = 10.24ISATRR61 pKa = 11.84MRR63 pKa = 11.84IILICVVLIVAAYY76 pKa = 9.54CLYY79 pKa = 10.57LIWGSLVEE87 pKa = 4.71LIKK90 pKa = 11.05

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4844

0

4844

1663111

25

5748

343.3

38.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.592 ± 0.046

0.729 ± 0.01

5.284 ± 0.019

5.604 ± 0.038

4.984 ± 0.033

6.806 ± 0.036

1.747 ± 0.019

7.288 ± 0.039

7.341 ± 0.042

9.508 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.018

5.925 ± 0.038

3.712 ± 0.022

3.76 ± 0.023

3.744 ± 0.025

6.458 ± 0.028

5.839 ± 0.077

6.192 ± 0.035

1.185 ± 0.014

4.16 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski