Amino acid dipepetide frequency for Gambusia affinis (Western mosquitofish) (Heterandria affinis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.929AlaAla: 6.929 ± 0.043	1.302AlaCys: 1.302 ± 0.012	3.242AlaAsp: 3.242 ± 0.019	4.781AlaGlu: 4.781 ± 0.027	2.359AlaPhe: 2.359 ± 0.015	4.517AlaGly: 4.517 ± 0.028	1.516AlaHis: 1.516 ± 0.012	2.485AlaIle: 2.485 ± 0.016	3.212AlaLys: 3.212 ± 0.024	6.457AlaLeu: 6.457 ± 0.034	1.531AlaMet: 1.531 ± 0.012	2.129AlaAsn: 2.129 ± 0.012	3.615AlaPro: 3.615 ± 0.027	2.877AlaGln: 2.877 ± 0.02	3.125AlaArg: 3.125 ± 0.017	5.641AlaSer: 5.641 ± 0.028	3.41AlaThr: 3.41 ± 0.019	5.069AlaVal: 5.069 ± 0.025	0.674AlaTrp: 0.674 ± 0.008	1.373AlaTyr: 1.373 ± 0.013	0.001AlaXaa: 0.001 ± 0.0
Cys	1.188CysAla: 1.188 ± 0.013	0.754CysCys: 0.754 ± 0.011	1.143CysAsp: 1.143 ± 0.015	1.253CysGlu: 1.253 ± 0.016	0.952CysPhe: 0.952 ± 0.01	1.66CysGly: 1.66 ± 0.022	0.655CysHis: 0.655 ± 0.008	0.936CysIle: 0.936 ± 0.012	1.128CysLys: 1.128 ± 0.013	2.274CysLeu: 2.274 ± 0.019	0.447CysMet: 0.447 ± 0.007	0.818CysAsn: 0.818 ± 0.012	1.336CysPro: 1.336 ± 0.016	1.044CysGln: 1.044 ± 0.013	1.427CysArg: 1.427 ± 0.013	2.426CysSer: 2.426 ± 0.019	1.121CysThr: 1.121 ± 0.013	1.544CysVal: 1.544 ± 0.016	0.329CysTrp: 0.329 ± 0.006	0.589CysTyr: 0.589 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.961AspAla: 2.961 ± 0.019	1.145AspCys: 1.145 ± 0.014	3.033AspAsp: 3.033 ± 0.025	3.724AspGlu: 3.724 ± 0.026	2.057AspPhe: 2.057 ± 0.014	3.757AspGly: 3.757 ± 0.027	1.153AspHis: 1.153 ± 0.011	2.45AspIle: 2.45 ± 0.018	2.605AspLys: 2.605 ± 0.019	4.909AspLeu: 4.909 ± 0.024	1.203AspMet: 1.203 ± 0.012	1.794AspAsn: 1.794 ± 0.017	2.881AspPro: 2.881 ± 0.016	2.005AspGln: 2.005 ± 0.014	2.84AspArg: 2.84 ± 0.02	4.503AspSer: 4.503 ± 0.023	2.418AspThr: 2.418 ± 0.018	3.336AspVal: 3.336 ± 0.021	0.679AspTrp: 0.679 ± 0.009	1.395AspTyr: 1.395 ± 0.01	0.001AspXaa: 0.001 ± 0.0
Glu	4.752GluAla: 4.752 ± 0.027	1.163GluCys: 1.163 ± 0.015	4.341GluAsp: 4.341 ± 0.027	7.84GluGlu: 7.84 ± 0.069	1.894GluPhe: 1.894 ± 0.014	3.974GluGly: 3.974 ± 0.025	1.42GluHis: 1.42 ± 0.013	2.769GluIle: 2.769 ± 0.019	4.697GluLys: 4.697 ± 0.038	6.029GluLeu: 6.029 ± 0.04	1.686GluMet: 1.686 ± 0.014	2.836GluAsn: 2.836 ± 0.021	2.999GluPro: 2.999 ± 0.024	3.07GluGln: 3.07 ± 0.025	4.334GluArg: 4.334 ± 0.033	4.56GluSer: 4.56 ± 0.027	3.591GluThr: 3.591 ± 0.021	4.202GluVal: 4.202 ± 0.022	0.668GluTrp: 0.668 ± 0.008	1.41GluTyr: 1.41 ± 0.014	0.001GluXaa: 0.001 ± 0.0
Phe	1.884PheAla: 1.884 ± 0.015	1.034PheCys: 1.034 ± 0.012	1.676PheAsp: 1.676 ± 0.013	1.775PheGlu: 1.775 ± 0.014	1.575PhePhe: 1.575 ± 0.016	2.168PheGly: 2.168 ± 0.017	1.056PheHis: 1.056 ± 0.011	1.869PheIle: 1.869 ± 0.015	1.742PheLys: 1.742 ± 0.016	3.975PheLeu: 3.975 ± 0.023	0.79PheMet: 0.79 ± 0.009	1.419PheAsn: 1.419 ± 0.011	1.983PhePro: 1.983 ± 0.015	1.637PheGln: 1.637 ± 0.013	1.949PheArg: 1.949 ± 0.016	3.655PheSer: 3.655 ± 0.022	2.261PheThr: 2.261 ± 0.016	2.205PheVal: 2.205 ± 0.016	0.498PheTrp: 0.498 ± 0.007	1.167PheTyr: 1.167 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	4.117GlyAla: 4.117 ± 0.025	1.331GlyCys: 1.331 ± 0.012	3.157GlyAsp: 3.157 ± 0.019	4.08GlyGlu: 4.08 ± 0.026	2.464GlyPhe: 2.464 ± 0.02	5.371GlyGly: 5.371 ± 0.042	1.638GlyHis: 1.638 ± 0.012	2.47GlyIle: 2.47 ± 0.018	3.448GlyLys: 3.448 ± 0.024	5.474GlyLeu: 5.474 ± 0.03	1.351GlyMet: 1.351 ± 0.014	2.422GlyAsn: 2.422 ± 0.018	3.232GlyPro: 3.232 ± 0.03	2.753GlyGln: 2.753 ± 0.019	3.888GlyArg: 3.888 ± 0.022	6.072GlySer: 6.072 ± 0.03	3.313GlyThr: 3.313 ± 0.022	4.006GlyVal: 4.006 ± 0.025	0.785GlyTrp: 0.785 ± 0.01	1.673GlyTyr: 1.673 ± 0.017	0.002GlyXaa: 0.002 ± 0.0
His	1.345HisAla: 1.345 ± 0.011	0.756HisCys: 0.756 ± 0.009	0.959HisAsp: 0.959 ± 0.01	1.161HisGlu: 1.161 ± 0.012	1.067HisPhe: 1.067 ± 0.009	1.573HisGly: 1.573 ± 0.014	1.111HisHis: 1.111 ± 0.014	1.246HisIle: 1.246 ± 0.012	1.285HisLys: 1.285 ± 0.012	2.835HisLeu: 2.835 ± 0.019	0.683HisMet: 0.683 ± 0.013	0.989HisAsn: 0.989 ± 0.011	1.633HisPro: 1.633 ± 0.015	1.394HisGln: 1.394 ± 0.012	1.779HisArg: 1.779 ± 0.013	2.522HisSer: 2.522 ± 0.016	1.564HisThr: 1.564 ± 0.018	1.491HisVal: 1.491 ± 0.011	0.349HisTrp: 0.349 ± 0.006	0.784HisTyr: 0.784 ± 0.01	0.0HisXaa: 0.0 ± 0.0
Ile	2.296IleAla: 2.296 ± 0.017	1.084IleCys: 1.084 ± 0.013	1.92IleAsp: 1.92 ± 0.016	2.189IleGlu: 2.189 ± 0.017	1.752IlePhe: 1.752 ± 0.014	2.217IleGly: 2.217 ± 0.016	1.262IleHis: 1.262 ± 0.012	2.292IleIle: 2.292 ± 0.019	2.461IleLys: 2.461 ± 0.02	4.164IleLeu: 4.164 ± 0.021	0.996IleMet: 0.996 ± 0.011	1.838IleAsn: 1.838 ± 0.015	2.418IlePro: 2.418 ± 0.017	2.134IleGln: 2.134 ± 0.015	2.456IleArg: 2.456 ± 0.014	3.787IleSer: 3.787 ± 0.02	2.617IleThr: 2.617 ± 0.02	2.415IleVal: 2.415 ± 0.02	0.483IleTrp: 0.483 ± 0.007	1.273IleTyr: 1.273 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	3.68LysAla: 3.68 ± 0.022	1.003LysCys: 1.003 ± 0.012	3.117LysAsp: 3.117 ± 0.023	4.64LysGlu: 4.64 ± 0.04	1.487LysPhe: 1.487 ± 0.012	3.055LysGly: 3.055 ± 0.029	1.433LysHis: 1.433 ± 0.011	2.388LysIle: 2.388 ± 0.02	4.346LysLys: 4.346 ± 0.035	4.893LysLeu: 4.893 ± 0.025	1.445LysMet: 1.445 ± 0.013	2.254LysAsn: 2.254 ± 0.019	3.015LysPro: 3.015 ± 0.025	2.584LysGln: 2.584 ± 0.023	3.509LysArg: 3.509 ± 0.022	3.905LysSer: 3.905 ± 0.022	3.298LysThr: 3.298 ± 0.02	3.416LysVal: 3.416 ± 0.019	0.569LysTrp: 0.569 ± 0.008	1.359LysTyr: 1.359 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	5.892LeuAla: 5.892 ± 0.031	2.338LeuCys: 2.338 ± 0.018	4.793LeuAsp: 4.793 ± 0.024	6.243LeuGlu: 6.243 ± 0.036	3.448LeuPhe: 3.448 ± 0.021	5.157LeuGly: 5.157 ± 0.025	2.847LeuHis: 2.847 ± 0.021	3.791LeuIle: 3.791 ± 0.02	5.549LeuLys: 5.549 ± 0.029	10.48LeuLeu: 10.48 ± 0.065	2.091LeuMet: 2.091 ± 0.016	3.585LeuAsn: 3.585 ± 0.022	5.532LeuPro: 5.532 ± 0.033	5.654LeuGln: 5.654 ± 0.04	5.889LeuArg: 5.889 ± 0.029	8.697LeuSer: 8.697 ± 0.034	5.423LeuThr: 5.423 ± 0.025	5.54LeuVal: 5.54 ± 0.027	1.129LeuTrp: 1.129 ± 0.011	2.442LeuTyr: 2.442 ± 0.019	0.002LeuXaa: 0.002 ± 0.0
Met	1.817MetAla: 1.817 ± 0.014	0.464MetCys: 0.464 ± 0.007	1.341MetAsp: 1.341 ± 0.012	1.942MetGlu: 1.942 ± 0.016	0.868MetPhe: 0.868 ± 0.009	1.312MetGly: 1.312 ± 0.014	0.466MetHis: 0.466 ± 0.007	0.836MetIle: 0.836 ± 0.01	1.465MetLys: 1.465 ± 0.01	2.019MetLeu: 2.019 ± 0.015	0.7MetMet: 0.7 ± 0.011	0.875MetAsn: 0.875 ± 0.009	1.026MetPro: 1.026 ± 0.011	0.952MetGln: 0.952 ± 0.01	1.212MetArg: 1.212 ± 0.012	1.894MetSer: 1.894 ± 0.013	1.259MetThr: 1.259 ± 0.01	1.451MetVal: 1.451 ± 0.013	0.266MetTrp: 0.266 ± 0.005	0.563MetTyr: 0.563 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.089AsnAla: 2.089 ± 0.014	0.854AsnCys: 0.854 ± 0.01	1.613AsnAsp: 1.613 ± 0.015	2.051AsnGlu: 2.051 ± 0.016	1.403AsnPhe: 1.403 ± 0.012	2.599AsnGly: 2.599 ± 0.021	1.014AsnHis: 1.014 ± 0.009	2.066AsnIle: 2.066 ± 0.015	2.199AsnLys: 2.199 ± 0.016	3.643AsnLeu: 3.643 ± 0.022	1.03AsnMet: 1.03 ± 0.01	1.74AsnAsn: 1.74 ± 0.016	2.267AsnPro: 2.267 ± 0.017	1.866AsnGln: 1.866 ± 0.015	2.121AsnArg: 2.121 ± 0.014	3.25AsnSer: 3.25 ± 0.019	2.182AsnThr: 2.182 ± 0.017	2.283AsnVal: 2.283 ± 0.016	0.455AsnTrp: 0.455 ± 0.006	1.037AsnTyr: 1.037 ± 0.011	0.0AsnXaa: 0.0 ± 0.0
Pro	4.395ProAla: 4.395 ± 0.032	1.125ProCys: 1.125 ± 0.013	2.974ProAsp: 2.974 ± 0.019	3.84ProGlu: 3.84 ± 0.023	1.873ProPhe: 1.873 ± 0.016	4.038ProGly: 4.038 ± 0.036	1.528ProHis: 1.528 ± 0.015	1.843ProIle: 1.843 ± 0.014	2.528ProLys: 2.528 ± 0.023	5.135ProLeu: 5.135 ± 0.024	0.966ProMet: 0.966 ± 0.011	1.945ProAsn: 1.945 ± 0.016	5.43ProPro: 5.43 ± 0.053	2.682ProGln: 2.682 ± 0.022	2.841ProArg: 2.841 ± 0.02	5.8ProSer: 5.8 ± 0.037	3.143ProThr: 3.143 ± 0.021	3.88ProVal: 3.88 ± 0.023	0.568ProTrp: 0.568 ± 0.008	1.32ProTyr: 1.32 ± 0.013	0.003ProXaa: 0.003 ± 0.0
Gln	3.11GlnAla: 3.11 ± 0.018	0.923GlnCys: 0.923 ± 0.011	2.305GlnAsp: 2.305 ± 0.015	3.532GlnGlu: 3.532 ± 0.029	1.327GlnPhe: 1.327 ± 0.011	2.591GlnGly: 2.591 ± 0.019	1.411GlnHis: 1.411 ± 0.014	1.943GlnIle: 1.943 ± 0.013	2.693GlnLys: 2.693 ± 0.023	4.605GlnLeu: 4.605 ± 0.03	1.136GlnMet: 1.136 ± 0.011	1.97GlnAsn: 1.97 ± 0.014	2.588GlnPro: 2.588 ± 0.022	3.414GlnGln: 3.414 ± 0.044	3.267GlnArg: 3.267 ± 0.021	3.657GlnSer: 3.657 ± 0.024	2.758GlnThr: 2.758 ± 0.019	2.816GlnVal: 2.816 ± 0.017	0.532GlnTrp: 0.532 ± 0.007	1.087GlnTyr: 1.087 ± 0.011	0.001GlnXaa: 0.001 ± 0.0
Arg	3.566ArgAla: 3.566 ± 0.021	1.399ArgCys: 1.399 ± 0.014	2.912ArgAsp: 2.912 ± 0.02	3.965ArgGlu: 3.965 ± 0.032	2.107ArgPhe: 2.107 ± 0.014	3.652ArgGly: 3.652 ± 0.023	1.671ArgHis: 1.671 ± 0.012	2.377ArgIle: 2.377 ± 0.016	3.652ArgLys: 3.652 ± 0.022	5.603ArgLeu: 5.603 ± 0.027	1.318ArgMet: 1.318 ± 0.012	2.189ArgAsn: 2.189 ± 0.014	3.159ArgPro: 3.159 ± 0.024	2.717ArgGln: 2.717 ± 0.02	4.901ArgArg: 4.901 ± 0.032	5.05ArgSer: 5.05 ± 0.032	3.169ArgThr: 3.169 ± 0.017	3.398ArgVal: 3.398 ± 0.023	0.746ArgTrp: 0.746 ± 0.009	1.465ArgTyr: 1.465 ± 0.012	0.001ArgXaa: 0.001 ± 0.0
Ser	5.999SerAla: 5.999 ± 0.026	2.275SerCys: 2.275 ± 0.021	4.548SerAsp: 4.548 ± 0.022	5.192SerGlu: 5.192 ± 0.03	3.308SerPhe: 3.308 ± 0.02	5.857SerGly: 5.857 ± 0.029	2.33SerHis: 2.33 ± 0.017	3.304SerIle: 3.304 ± 0.019	4.09SerLys: 4.09 ± 0.026	8.688SerLeu: 8.688 ± 0.034	1.75SerMet: 1.75 ± 0.014	3.078SerAsn: 3.078 ± 0.017	6.156SerPro: 6.156 ± 0.04	4.015SerGln: 4.015 ± 0.024	4.975SerArg: 4.975 ± 0.03	11.257SerSer: 11.257 ± 0.066	4.826SerThr: 4.826 ± 0.024	5.729SerVal: 5.729 ± 0.026	1.085SerTrp: 1.085 ± 0.012	2.066SerTyr: 2.066 ± 0.015	0.001SerXaa: 0.001 ± 0.0
Thr	4.092ThrAla: 4.092 ± 0.022	1.379ThrCys: 1.379 ± 0.018	2.836ThrAsp: 2.836 ± 0.015	3.814ThrGlu: 3.814 ± 0.022	2.1ThrPhe: 2.1 ± 0.015	3.755ThrGly: 3.755 ± 0.023	1.427ThrHis: 1.427 ± 0.013	2.274ThrIle: 2.274 ± 0.018	2.697ThrLys: 2.697 ± 0.02	5.274ThrLeu: 5.274 ± 0.022	1.193ThrMet: 1.193 ± 0.011	1.95ThrAsn: 1.95 ± 0.016	3.576ThrPro: 3.576 ± 0.023	2.394ThrGln: 2.394 ± 0.016	2.671ThrArg: 2.671 ± 0.017	4.993ThrSer: 4.993 ± 0.023	3.217ThrThr: 3.217 ± 0.026	4.06ThrVal: 4.06 ± 0.024	0.68ThrTrp: 0.68 ± 0.008	1.314ThrTyr: 1.314 ± 0.013	0.001ThrXaa: 0.001 ± 0.0
Val	4.159ValAla: 4.159 ± 0.023	1.766ValCys: 1.766 ± 0.021	3.075ValAsp: 3.075 ± 0.017	4.032ValGlu: 4.032 ± 0.022	2.666ValPhe: 2.666 ± 0.016	3.624ValGly: 3.624 ± 0.022	1.581ValHis: 1.581 ± 0.013	2.852ValIle: 2.852 ± 0.022	3.507ValLys: 3.507 ± 0.022	6.396ValLeu: 6.396 ± 0.036	1.456ValMet: 1.456 ± 0.012	2.346ValAsn: 2.346 ± 0.019	3.341ValPro: 3.341 ± 0.021	2.839ValGln: 2.839 ± 0.016	3.408ValArg: 3.408 ± 0.021	5.616ValSer: 5.616 ± 0.027	3.908ValThr: 3.908 ± 0.023	4.382ValVal: 4.382 ± 0.027	0.8ValTrp: 0.8 ± 0.009	1.666ValTyr: 1.666 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.674TrpAla: 0.674 ± 0.008	0.256TrpCys: 0.256 ± 0.005	0.633TrpAsp: 0.633 ± 0.008	0.75TrpGlu: 0.75 ± 0.009	0.499TrpPhe: 0.499 ± 0.007	0.639TrpGly: 0.639 ± 0.009	0.265TrpHis: 0.265 ± 0.005	0.57TrpIle: 0.57 ± 0.008	0.74TrpLys: 0.74 ± 0.009	1.194TrpLeu: 1.194 ± 0.013	0.349TrpMet: 0.349 ± 0.006	0.488TrpAsn: 0.488 ± 0.007	0.453TrpPro: 0.453 ± 0.005	0.488TrpGln: 0.488 ± 0.007	0.834TrpArg: 0.834 ± 0.009	1.016TrpSer: 1.016 ± 0.011	0.757TrpThr: 0.757 ± 0.009	0.726TrpVal: 0.726 ± 0.008	0.195TrpTrp: 0.195 ± 0.004	0.331TrpTyr: 0.331 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.298TyrAla: 1.298 ± 0.012	0.674TyrCys: 0.674 ± 0.007	1.23TyrAsp: 1.23 ± 0.012	1.429TyrGlu: 1.429 ± 0.011	1.111TyrPhe: 1.111 ± 0.012	1.531TyrGly: 1.531 ± 0.013	0.742TyrHis: 0.742 ± 0.008	1.294TyrIle: 1.294 ± 0.013	1.354TyrLys: 1.354 ± 0.013	2.474TyrLeu: 2.474 ± 0.016	0.607TyrMet: 0.607 ± 0.006	1.098TyrAsn: 1.098 ± 0.012	1.214TyrPro: 1.214 ± 0.013	1.147TyrGln: 1.147 ± 0.01	1.588TyrArg: 1.588 ± 0.013	2.227TyrSer: 2.227 ± 0.016	1.459TyrThr: 1.459 ± 0.014	1.475TyrVal: 1.475 ± 0.013	0.363TyrTrp: 0.363 ± 0.006	0.865TyrTyr: 0.865 ± 0.011	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.052XaaXaa: 0.052 ± 0.004

Statistics based on 19443 proteins (11554075 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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