Pyrobaculum filamentous virus 2

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Primavirales; Tristromaviridae; Alphatristromavirus; Alphatristromavirus PFV2

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3VXP5|A0A6M3VXP5_9VIRU Uncharacterized protein OS=Pyrobaculum filamentous virus 2 OX=2730621 GN=PFV2_gp14 PE=4 SV=1
MM1 pKa = 7.68IDD3 pKa = 3.89EE4 pKa = 4.74PLEE7 pKa = 4.38APSVSCEE14 pKa = 3.92EE15 pKa = 3.88LMEE18 pKa = 3.88MCMRR22 pKa = 11.84VGGMWCEE29 pKa = 5.18DD30 pKa = 3.17YY31 pKa = 11.17DD32 pKa = 4.14ALCGEE37 pKa = 4.95DD38 pKa = 3.39VV39 pKa = 3.39

Molecular weight: 4.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3VZ93|A0A6M3VZ93_9VIRU Uncharacterized protein OS=Pyrobaculum filamentous virus 2 OX=2730621 GN=PFV2_gp22 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.04LVKK5 pKa = 10.58GFVEE9 pKa = 4.62RR10 pKa = 11.84MRR12 pKa = 11.84SVAEE16 pKa = 3.96VLSDD20 pKa = 3.67RR21 pKa = 11.84RR22 pKa = 11.84WIEE25 pKa = 3.23FWDD28 pKa = 3.74NVNRR32 pKa = 11.84MLDD35 pKa = 3.39SGDD38 pKa = 2.74WRR40 pKa = 11.84FRR42 pKa = 11.84AVVKK46 pKa = 10.39KK47 pKa = 9.86VYY49 pKa = 10.33KK50 pKa = 10.39VIYY53 pKa = 9.86

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

5520

39

545

141.5

16.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.467 ± 0.51

1.938 ± 0.283

5.96 ± 0.368

6.159 ± 0.604

4.42 ± 0.356

7.591 ± 0.517

1.359 ± 0.192

6.667 ± 0.402

5.29 ± 0.425

7.138 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.152 ± 0.347

3.514 ± 0.374

3.351 ± 0.479

1.522 ± 0.186

6.141 ± 0.474

5.217 ± 0.366

4.928 ± 0.771

11.359 ± 0.601

2.138 ± 0.179

5.688 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski